Click here to switch to the map view.

The map label for this gene is ntrC [H]

Identifier: 16125985

GI number: 16125985

Start: 1913850

End: 1915271

Strand: Direct

Name: ntrC [H]

Synonym: CC_1741

Alternate gene names: 16125985

Gene position: 1913850-1915271 (Clockwise)

Preceding gene: 16125984

Following gene: 16125986

Centisome position: 47.64

GC content: 66.6

Gene sequence:

>1422_bases
ATGAACGCCGCGAGCAAGAAAATCCTGATCGCCGACGACGACAGCTCTATCCGTCTCGTCCTCAGTCAAGCCTTCACCCG
CCTGGGCTACCAGGTCCGCGCCACGGGTAACGCGACGACATTGTTGAAGTGGGTGACCGACGGCGAGGGCGATCTCGTCG
TGACCGATGTGATGATGCCTGACGAGAACGTCTTCGACGTTCTGCCCAGGATCCGGAAGGAGCGGCCCAAGCTTCCGATC
ATCGTCATGAGTGCGCAGAACACCCTTCTGACGGCCGTCAACGCCGCCGACGCGGGCGCGTTCGAATACGTCTCCAAGCC
GTTCGACCTTGATGACGTTACGGCTGCGGCGCGGCGCGCCTTGTCGCGGCCGGCGGACACTGAGGCCTCCAAGGCCCAGG
CGCGGGCCATGCGCGACGAGCGCCTCCCACTCATCGGGCGTTCGGCGCCGATGCAGGAGGTCTACCGCACCATCGCGCGC
CTGGTCGGTGCTGATCTGACGGTTCTGATCCTCGGCGAAAGCGGCACCGGCAAGGAACTGGTGGCGCGCGCCCTGCACGA
GCTTGGCCGTCGTCGCGATGGCAAATTCGTTGTCATCAACCTGGCGGCCGTGCCGCGTGAGCGGGTCGAGGCCGAGCTGT
TCGGACGCGGGGAAGGGGACAACGGCCGGCTGGTCGAGGCCGACGGCGGCACGCTGTTCCTCGACGAGATTGGCGACATG
CCCCTGGATGCCCAGACGCGTCTGCTTCGCGTGATCGATGGCACCGAGCCGGTGATCAACCCGAAGACCGGCCGTCGGCC
GAACGTGCGCATCATCGCCGCGACCAATCGCGACCTGCGCGGCCTGATCCAGCAGGGCCTGTTCCGCGAGGATCTCTTCT
TCCGCCTGAACGTCGCGCCCGTCCGCTTGCCGCCGCTACGCGATCGCGCCGAAGACATTCCCGATCTGGCGCGCACCTTC
CTGCTTCGAGCCGCGCGGGAGGGCCTGCCGGCCAAGACCATCGACCAAGGCGCCCTGGACCGTCTGAAGAGTCATCCCTG
GCCGGGGAACGTGCGCGAACTCGAAAACCTGATGCGTCGGATGTGCGCGCTCTACGCCGAAGAGCTGATCACCTCCCGCA
TCGTGGACCGGGAGCTGCAGGATCACGCGCCTGCGGTACGGGCCGAAGAAGGGCCTGTGACGCTTTCGACCCTGGTGGAG
CGTCATCTGGCGTCGCACTTCGCCGATCAGCCTGACGGCGTCCCGGCGCCGGGGCTCTATGATCGGATTCTGGAGGAGGT
GGAGAGGCCGCTGATCCAGCTGACGCTGTCGGCGACGCGCGGCAACCAGGTGCGCGCCGCGGAGATCTTGGGCCTCAACC
GCAACACGCTGCGCAAGAAGATCCAGGACCTTGGCGTGGAGATGACCCGCGGTCGTCGCTGA

Upstream 100 bases:

>100_bases
CCCACGGCGGTCTGATCGATTTTGAATCGGAGCCCGGCCGAACGGTCTTCCGCGTCTTGTTGCCTGTTGCCCCTGAAGCC
GCTTCCGGAGACGTCCGAGC

Downstream 100 bases:

>100_bases
CGGCTGCAGCTTTCGCGGCACATAACTGTTGAATTTGAGCCACACGCTCCTAAACTCGTGAGCGATGTCTTCAGTGGCTT
ACGCGACGGGATCCGATAGC

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVTDGEGDLVVTDVMMPDENVFDVLPRIRKERPKLPI
IVMSAQNTLLTAVNAADAGAFEYVSKPFDLDDVTAAARRALSRPADTEASKAQARAMRDERLPLIGRSAPMQEVYRTIAR
LVGADLTVLILGESGTGKELVARALHELGRRRDGKFVVINLAAVPRERVEAELFGRGEGDNGRLVEADGGTLFLDEIGDM
PLDAQTRLLRVIDGTEPVINPKTGRRPNVRIIAATNRDLRGLIQQGLFREDLFFRLNVAPVRLPPLRDRAEDIPDLARTF
LLRAAREGLPAKTIDQGALDRLKSHPWPGNVRELENLMRRMCALYAEELITSRIVDRELQDHAPAVRAEEGPVTLSTLVE
RHLASHFADQPDGVPAPGLYDRILEEVERPLIQLTLSATRGNQVRAAEILGLNRNTLRKKIQDLGVEMTRGRR

Sequences:

>Translated_473_residues
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVTDGEGDLVVTDVMMPDENVFDVLPRIRKERPKLPI
IVMSAQNTLLTAVNAADAGAFEYVSKPFDLDDVTAAARRALSRPADTEASKAQARAMRDERLPLIGRSAPMQEVYRTIAR
LVGADLTVLILGESGTGKELVARALHELGRRRDGKFVVINLAAVPRERVEAELFGRGEGDNGRLVEADGGTLFLDEIGDM
PLDAQTRLLRVIDGTEPVINPKTGRRPNVRIIAATNRDLRGLIQQGLFREDLFFRLNVAPVRLPPLRDRAEDIPDLARTF
LLRAAREGLPAKTIDQGALDRLKSHPWPGNVRELENLMRRMCALYAEELITSRIVDRELQDHAPAVRAEEGPVTLSTLVE
RHLASHFADQPDGVPAPGLYDRILEEVERPLIQLTLSATRGNQVRAAEILGLNRNTLRKKIQDLGVEMTRGRR
>Mature_473_residues
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVTDGEGDLVVTDVMMPDENVFDVLPRIRKERPKLPI
IVMSAQNTLLTAVNAADAGAFEYVSKPFDLDDVTAAARRALSRPADTEASKAQARAMRDERLPLIGRSAPMQEVYRTIAR
LVGADLTVLILGESGTGKELVARALHELGRRRDGKFVVINLAAVPRERVEAELFGRGEGDNGRLVEADGGTLFLDEIGDM
PLDAQTRLLRVIDGTEPVINPKTGRRPNVRIIAATNRDLRGLIQQGLFREDLFFRLNVAPVRLPPLRDRAEDIPDLARTF
LLRAAREGLPAKTIDQGALDRLKSHPWPGNVRELENLMRRMCALYAEELITSRIVDRELQDHAPAVRAEEGPVTLSTLVE
RHLASHFADQPDGVPAPGLYDRILEEVERPLIQLTLSATRGNQVRAAEILGLNRNTLRKKIQDLGVEMTRGRR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=474, Percent_Identity=40.7172995780591, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788550, Length=479, Percent_Identity=36.1169102296451, Blast_Score=275, Evalue=7e-75,
Organism=Escherichia coli, GI1790437, Length=471, Percent_Identity=37.1549893842887, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1788905, Length=471, Percent_Identity=33.5456475583864, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789233, Length=331, Percent_Identity=38.9728096676737, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87082117, Length=233, Percent_Identity=49.7854077253219, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789087, Length=242, Percent_Identity=44.6280991735537, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=43.2900432900433, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87081872, Length=334, Percent_Identity=34.7305389221557, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786524, Length=256, Percent_Identity=42.1875, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787583, Length=220, Percent_Identity=39.5454545454545, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=30.6962025316456, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=35.3448275862069, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=31.9672131147541, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790552, Length=125, Percent_Identity=32.8, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52411; Mature: 52411

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVTDGEGDLVVTDVMMP
CCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCCEEEEEEECC
DENVFDVLPRIRKERPKLPIIVMSAQNTLLTAVNAADAGAFEYVSKPFDLDDVTAAARRA
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHH
LSRPADTEASKAQARAMRDERLPLIGRSAPMQEVYRTIARLVGADLTVLILGESGTGKEL
HCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHH
VARALHELGRRRDGKFVVINLAAVPRERVEAELFGRGEGDNGRLVEADGGTLFLDEIGDM
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCEEEHHHHCCC
PLDAQTRLLRVIDGTEPVINPKTGRRPNVRIIAATNRDLRGLIQQGLFREDLFFRLNVAP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHCCEEEEECCC
VRLPPLRDRAEDIPDLARTFLLRAAREGLPAKTIDQGALDRLKSHPWPGNVRELENLMRR
EECCCCHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MCALYAEELITSRIVDRELQDHAPAVRAEEGPVTLSTLVERHLASHFADQPDGVPAPGLY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
DRILEEVERPLIQLTLSATRGNQVRAAEILGLNRNTLRKKIQDLGVEMTRGRR
HHHHHHHCCCCEEEEEECCCCCCEEEHHHCCCCHHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVTDGEGDLVVTDVMMP
CCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCCEEEEEEECC
DENVFDVLPRIRKERPKLPIIVMSAQNTLLTAVNAADAGAFEYVSKPFDLDDVTAAARRA
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHH
LSRPADTEASKAQARAMRDERLPLIGRSAPMQEVYRTIARLVGADLTVLILGESGTGKEL
HCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHH
VARALHELGRRRDGKFVVINLAAVPRERVEAELFGRGEGDNGRLVEADGGTLFLDEIGDM
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCEEEHHHHCCC
PLDAQTRLLRVIDGTEPVINPKTGRRPNVRIIAATNRDLRGLIQQGLFREDLFFRLNVAP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHCCEEEEECCC
VRLPPLRDRAEDIPDLARTFLLRAAREGLPAKTIDQGALDRLKSHPWPGNVRELENLMRR
EECCCCHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MCALYAEELITSRIVDRELQDHAPAVRAEEGPVTLSTLVERHLASHFADQPDGVPAPGLY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
DRILEEVERPLIQLTLSATRGNQVRAAEILGLNRNTLRKKIQDLGVEMTRGRR
HHHHHHHCCCCEEEEEECCCCCCEEEHHHCCCCHHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]