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The map label for this gene is tgt

Identifier: 16125835

GI number: 16125835

Start: 1754017

End: 1755132

Strand: Direct

Name: tgt

Synonym: CC_1588

Alternate gene names: 16125835

Gene position: 1754017-1755132 (Clockwise)

Preceding gene: 16125834

Following gene: 16125837

Centisome position: 43.67

GC content: 66.67

Gene sequence:

>1116_bases
ATGGCAGCGTTCCCCTTCGAGATCGAAGCCACGGACGGCAAGGCGCGCACCGGCGTGCTCAAGACCCCGCGCGGCGACAT
CCGCACGCCGGCCTTCATGCCGGTCGGCACCGCCGCGACGGTCAAGGCCATGACGGTCGATCAGGTCAAGGACACCGGCG
CCGACATCATCCTGGGCAACACCTATCACCTGATGCTGCGGCCCTCGGCTGAGCGGGTGAAGCGCCTGGGCGGGCTTCAT
AAGTTCATGCGCTGGGACAAGCCGATCCTGACCGACAGCGGCGGCTTCCAGGTGATGAGCCTGTCAGGGATCAGCAAGCT
GACCGAGGAGGCGGTGACCTTCTCCAGCCACGTCGACGGCTCCAAGCATGTGCTGACGCCTGAGCGTTCGATCGAAATCC
AGGCCGACCTTCTGGGCAGCGACATCGTCATGCAACTGGACGAGTGCGTCGCCTGGCCGGCGGAAGAGGCGAGGGCGCGC
AAGGGCATGGAGCTTTCGGCGCGCTGGGCCAAGCGCTCGAAGGACGCCTTCGGAACCCGCGACACCCAGGTCCTGTTCGG
CATCCAGCAGGGCTCGACCTTCGAAAATCTGCGTCGCGAGTCGTCCGAGCGCCTGCGCGAGATCGGCTTCGACGGCTACG
CCATCGGCGGCCTGGCGGTCGGCGAGGGTCACCAGGCGATGTGCGAGGTGTTGGACTATGCGCCGGGTTTCCTGCCTGAA
GACCGCCCGCGCTATCTGATGGGCGTGGGCAAGCCGATCGATCTGGTCGAAGCGGTGGCCCGAGGCGTCGACATGTTCGA
CTGCGTGCTGCCCACGCGCTCCGGCCGCCATGGTCAGGCCTGGACCTGGGATGGTCCCATCAACCTGAAGAACGCCAAGT
ACGCCGAGGACGAGACGCCGCTGGATCCCGACAGCGACTGCCCCGCCAGCCGCGACTATTCGAAGGCCTATCTGCGCCAC
CTCTTCAAGGCCGAGGAAATCCTCGGCCAGGTGCTGCTGTCCTGGCACAATATCGCGTTCTTCCAGGCGCTGACCGCCGC
CATGCGCGCGGCGATCGCGGAGGGGCGCTTCGAGCAGTTCCGGCGCGATTTCGCCGCGCGACACCTCGGCGGTTAG

Upstream 100 bases:

>100_bases
CCGAGGCCATGCGCGCGGCCTATGAGCATGCGATCGCGACGGGTTACCGCTTCTATTCCTACGGCGACAGCAGTCTGCTG
TTCAAAGACGAGACCTGCTG

Downstream 100 bases:

>100_bases
AGCCCTTTAGCTTTAGATGGAATCATCTAAAGCGTTTAAAAGAGCTCTAAGTATTTGATTTTAGAGCCTGTTTATCTGGT
TTAGATGGTTCCGTCTAAAC

Product: queuine tRNA ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MAAFPFEIEATDGKARTGVLKTPRGDIRTPAFMPVGTAATVKAMTVDQVKDTGADIILGNTYHLMLRPSAERVKRLGGLH
KFMRWDKPILTDSGGFQVMSLSGISKLTEEAVTFSSHVDGSKHVLTPERSIEIQADLLGSDIVMQLDECVAWPAEEARAR
KGMELSARWAKRSKDAFGTRDTQVLFGIQQGSTFENLRRESSERLREIGFDGYAIGGLAVGEGHQAMCEVLDYAPGFLPE
DRPRYLMGVGKPIDLVEAVARGVDMFDCVLPTRSGRHGQAWTWDGPINLKNAKYAEDETPLDPDSDCPASRDYSKAYLRH
LFKAEEILGQVLLSWHNIAFFQALTAAMRAAIAEGRFEQFRRDFAARHLGG

Sequences:

>Translated_371_residues
MAAFPFEIEATDGKARTGVLKTPRGDIRTPAFMPVGTAATVKAMTVDQVKDTGADIILGNTYHLMLRPSAERVKRLGGLH
KFMRWDKPILTDSGGFQVMSLSGISKLTEEAVTFSSHVDGSKHVLTPERSIEIQADLLGSDIVMQLDECVAWPAEEARAR
KGMELSARWAKRSKDAFGTRDTQVLFGIQQGSTFENLRRESSERLREIGFDGYAIGGLAVGEGHQAMCEVLDYAPGFLPE
DRPRYLMGVGKPIDLVEAVARGVDMFDCVLPTRSGRHGQAWTWDGPINLKNAKYAEDETPLDPDSDCPASRDYSKAYLRH
LFKAEEILGQVLLSWHNIAFFQALTAAMRAAIAEGRFEQFRRDFAARHLGG
>Mature_370_residues
AAFPFEIEATDGKARTGVLKTPRGDIRTPAFMPVGTAATVKAMTVDQVKDTGADIILGNTYHLMLRPSAERVKRLGGLHK
FMRWDKPILTDSGGFQVMSLSGISKLTEEAVTFSSHVDGSKHVLTPERSIEIQADLLGSDIVMQLDECVAWPAEEARARK
GMELSARWAKRSKDAFGTRDTQVLFGIQQGSTFENLRRESSERLREIGFDGYAIGGLAVGEGHQAMCEVLDYAPGFLPED
RPRYLMGVGKPIDLVEAVARGVDMFDCVLPTRSGRHGQAWTWDGPINLKNAKYAEDETPLDPDSDCPASRDYSKAYLRHL
FKAEEILGQVLLSWHNIAFFQALTAAMRAAIAEGRFEQFRRDFAARHLGG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=362, Percent_Identity=43.3701657458564, Blast_Score=300, Evalue=1e-81,
Organism=Escherichia coli, GI1786607, Length=364, Percent_Identity=54.1208791208791, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17544680, Length=366, Percent_Identity=41.5300546448087, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=45.7063711911357, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=22.65625, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_CAUCR (Q9A7Y1)

Other databases:

- EMBL:   AE005673
- PIR:   C87446
- RefSeq:   NP_420399.1
- ProteinModelPortal:   Q9A7Y1
- SMR:   Q9A7Y1
- GeneID:   941943
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_1588
- NMPDR:   fig|190650.1.peg.1579
- TIGR:   CC_1588
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41009; Mature: 40878

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: ACT_SITE 92-92 BINDING 93-93

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFPFEIEATDGKARTGVLKTPRGDIRTPAFMPVGTAATVKAMTVDQVKDTGADIILGN
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECC
TYHLMLRPSAERVKRLGGLHKFMRWDKPILTDSGGFQVMSLSGISKLTEEAVTFSSHVDG
EEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECHHHHHHHHHHHHHHCCCC
SKHVLTPERSIEIQADLLGSDIVMQLDECVAWPAEEARARKGMELSARWAKRSKDAFGTR
CCEEECCCCCEEEEHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCC
DTQVLFGIQQGSTFENLRRESSERLREIGFDGYAIGGLAVGEGHQAMCEVLDYAPGFLPE
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHCCCCCCC
DRPRYLMGVGKPIDLVEAVARGVDMFDCVLPTRSGRHGQAWTWDGPINLKNAKYAEDETP
CCCCCEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
LDPDSDCPASRDYSKAYLRHLFKAEEILGQVLLSWHNIAFFQALTAAMRAAIAEGRFEQF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRDFAARHLGG
HHHHHHHHCCC
>Mature Secondary Structure 
AAFPFEIEATDGKARTGVLKTPRGDIRTPAFMPVGTAATVKAMTVDQVKDTGADIILGN
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECC
TYHLMLRPSAERVKRLGGLHKFMRWDKPILTDSGGFQVMSLSGISKLTEEAVTFSSHVDG
EEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECHHHHHHHHHHHHHHCCCC
SKHVLTPERSIEIQADLLGSDIVMQLDECVAWPAEEARARKGMELSARWAKRSKDAFGTR
CCEEECCCCCEEEEHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCC
DTQVLFGIQQGSTFENLRRESSERLREIGFDGYAIGGLAVGEGHQAMCEVLDYAPGFLPE
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHCCCCCCC
DRPRYLMGVGKPIDLVEAVARGVDMFDCVLPTRSGRHGQAWTWDGPINLKNAKYAEDETP
CCCCCEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
LDPDSDCPASRDYSKAYLRHLFKAEEILGQVLLSWHNIAFFQALTAAMRAAIAEGRFEQF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRDFAARHLGG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647