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The map label for this gene is queA

Identifier: 16125834

GI number: 16125834

Start: 1752917

End: 1754017

Strand: Direct

Name: queA

Synonym: CC_1587

Alternate gene names: 16125834

Gene position: 1752917-1754017 (Clockwise)

Preceding gene: 16125831

Following gene: 16125835

Centisome position: 43.64

GC content: 67.03

Gene sequence:

>1101_bases
ATGCGACTTTCCGATTTTGATTTCGACCTTCCTGAGGACCGCATCGCGTTGCGACCCGCAGAGCCCCGCGACTCAGCGCG
CTTTCTGGTCGTGCGCCCTGGTCAGGCTGTAGCTGATCACATCGTCAGCGACCTGCCGGACTTCCTGCGGCCGGGCGATG
CTCTGGTGTTCAACGACACGCGGGTGATCCCAGCGCGCCTTTCCGGTCTTCGCGAAGGGCGCACGACGGGCGGCGCCGAC
GGTACGCCCGTGGCCGTCGAGGCCACCTTGCACCGCCGCCTGGCGCCCGATCGTTGGAGCGCCTTCATGCGCCCCGGCAA
GCGTCTGAAGGTCGGCGATCGGGTCGCGTTCGGCGGACACGAAGGCCGCGTCGGCGATCTTGGCCGCCTGGACGCCGTGA
TCGCCGAGAAGCATGACGGCGGTGAAGTCGTCCTGGCGTTCGACCTTTCTGGTCCGGACCTGGATGTCGGCATCGCCCAG
CATGGCGACATGCCGCTGCCGCCCTATATCGCCGCCAAGCGGGGCGAGGACGAGCGCGATCGCGCCGACTACCAGACGGT
CTATGCCCGCGAGGACGGTTCTGTCGCCGCCCCGACCGCGGGGCTGCACTTCACGCCCCAGCTCCTGGAGCGCCTGAAGG
CCAAGGGCGTTTCGCTCCACTTCGTCACGCTGCATGTCGGGGCGGGCACCTTCCTGCCCGTCAAGACCGACGAGGTCTCC
GAACACAGGATGCACGCCGAATACGGTCAGGTGACGCAAGAGATCGCCGACGCCCTGAACGCCGCGCGCGCGGCCGGCGG
ACGCATCGTCTGCGTCGGCACCACCTCGTTGCGGCTGCTGGAGAGCGCGACGGGCGAAGACGGGATTGTGCGGCCGTTCG
CCGACGAGACTGCGATCTTCATCACGCCCGGCTACCGGTTCCGGACGGCTGATGTGCTGATGACGAACTTCCACCTGCCG
AAGTCGACCCTGTTCATGCTGGTCAGCGCCTTCGCCGGGACCGAGGCCATGCGCGCGGCCTATGAGCATGCGATCGCGAC
GGGTTACCGCTTCTATTCCTACGGCGACAGCAGTCTGCTGTTCAAAGACGAGACCTGCTGA

Upstream 100 bases:

>100_bases
TTCGCAGCGAGCATGCGATCTTGCGAGCGGCGTTGTCTACCGAGCCACGAAGACGGGCGCGTCTTGACCTTGGGCGGTCA
TCGTGTGCTTGAAGCCGCCC

Downstream 100 bases:

>100_bases
TGGCAGCGTTCCCCTTCGAGATCGAAGCCACGGACGGCAAGGCGCGCACCGGCGTGCTCAAGACCCCGCGCGGCGACATC
CGCACGCCGGCCTTCATGCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRLSDFDFDLPEDRIALRPAEPRDSARFLVVRPGQAVADHIVSDLPDFLRPGDALVFNDTRVIPARLSGLREGRTTGGAD
GTPVAVEATLHRRLAPDRWSAFMRPGKRLKVGDRVAFGGHEGRVGDLGRLDAVIAEKHDGGEVVLAFDLSGPDLDVGIAQ
HGDMPLPPYIAAKRGEDERDRADYQTVYAREDGSVAAPTAGLHFTPQLLERLKAKGVSLHFVTLHVGAGTFLPVKTDEVS
EHRMHAEYGQVTQEIADALNAARAAGGRIVCVGTTSLRLLESATGEDGIVRPFADETAIFITPGYRFRTADVLMTNFHLP
KSTLFMLVSAFAGTEAMRAAYEHAIATGYRFYSYGDSSLLFKDETC

Sequences:

>Translated_366_residues
MRLSDFDFDLPEDRIALRPAEPRDSARFLVVRPGQAVADHIVSDLPDFLRPGDALVFNDTRVIPARLSGLREGRTTGGAD
GTPVAVEATLHRRLAPDRWSAFMRPGKRLKVGDRVAFGGHEGRVGDLGRLDAVIAEKHDGGEVVLAFDLSGPDLDVGIAQ
HGDMPLPPYIAAKRGEDERDRADYQTVYAREDGSVAAPTAGLHFTPQLLERLKAKGVSLHFVTLHVGAGTFLPVKTDEVS
EHRMHAEYGQVTQEIADALNAARAAGGRIVCVGTTSLRLLESATGEDGIVRPFADETAIFITPGYRFRTADVLMTNFHLP
KSTLFMLVSAFAGTEAMRAAYEHAIATGYRFYSYGDSSLLFKDETC
>Mature_366_residues
MRLSDFDFDLPEDRIALRPAEPRDSARFLVVRPGQAVADHIVSDLPDFLRPGDALVFNDTRVIPARLSGLREGRTTGGAD
GTPVAVEATLHRRLAPDRWSAFMRPGKRLKVGDRVAFGGHEGRVGDLGRLDAVIAEKHDGGEVVLAFDLSGPDLDVGIAQ
HGDMPLPPYIAAKRGEDERDRADYQTVYAREDGSVAAPTAGLHFTPQLLERLKAKGVSLHFVTLHVGAGTFLPVKTDEVS
EHRMHAEYGQVTQEIADALNAARAAGGRIVCVGTTSLRLLESATGEDGIVRPFADETAIFITPGYRFRTADVLMTNFHLP
KSTLFMLVSAFAGTEAMRAAYEHAIATGYRFYSYGDSSLLFKDETC

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=363, Percent_Identity=46.0055096418733, Blast_Score=291, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_CAUCN (B8GVF3)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002517031.1
- ProteinModelPortal:   B8GVF3
- SMR:   B8GVF3
- GeneID:   7331717
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01658
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39817; Mature: 39817

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSDFDFDLPEDRIALRPAEPRDSARFLVVRPGQAVADHIVSDLPDFLRPGDALVFNDT
CCCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECC
RVIPARLSGLREGRTTGGADGTPVAVEATLHRRLAPDRWSAFMRPGKRLKVGDRVAFGGH
EEEHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCCCHHHHHHHCCCCEEECCCEEEECCC
EGRVGDLGRLDAVIAEKHDGGEVVLAFDLSGPDLDVGIAQHGDMPLPPYIAAKRGEDERD
CCCCCCCHHHHHHEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHH
RADYQTVYAREDGSVAAPTAGLHFTPQLLERLKAKGVSLHFVTLHVGAGTFLPVKTDEVS
HCCCEEEEEECCCCEECCCCCCEECHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCHH
EHRMHAEYGQVTQEIADALNAARAAGGRIVCVGTTSLRLLESATGEDGIVRPFADETAIF
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEEEE
ITPGYRFRTADVLMTNFHLPKSTLFMLVSAFAGTEAMRAAYEHAIATGYRFYSYGDSSLL
EECCCEEEEHEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCEEEEEECCCEEE
FKDETC
EECCCC
>Mature Secondary Structure
MRLSDFDFDLPEDRIALRPAEPRDSARFLVVRPGQAVADHIVSDLPDFLRPGDALVFNDT
CCCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECC
RVIPARLSGLREGRTTGGADGTPVAVEATLHRRLAPDRWSAFMRPGKRLKVGDRVAFGGH
EEEHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCCCHHHHHHHCCCCEEECCCEEEECCC
EGRVGDLGRLDAVIAEKHDGGEVVLAFDLSGPDLDVGIAQHGDMPLPPYIAAKRGEDERD
CCCCCCCHHHHHHEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHH
RADYQTVYAREDGSVAAPTAGLHFTPQLLERLKAKGVSLHFVTLHVGAGTFLPVKTDEVS
HCCCEEEEEECCCCEECCCCCCEECHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCHH
EHRMHAEYGQVTQEIADALNAARAAGGRIVCVGTTSLRLLESATGEDGIVRPFADETAIF
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEEEE
ITPGYRFRTADVLMTNFHLPKSTLFMLVSAFAGTEAMRAAYEHAIATGYRFYSYGDSSLL
EECCCEEEEHEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCEEEEEECCCEEE
FKDETC
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA