Click here to switch to the map view.

The map label for this gene is fixI [H]

Identifier: 16125656

GI number: 16125656

Start: 1554154

End: 1556328

Strand: Direct

Name: fixI [H]

Synonym: CC_1407

Alternate gene names: 16125656

Gene position: 1554154-1556328 (Clockwise)

Preceding gene: 16125655

Following gene: 16125657

Centisome position: 38.69

GC content: 70.8

Gene sequence:

>2175_bases
ATGAGCCACAGCCTCGCCCTGCATCGTGACCTGGAGGCCTTCGTTCGGCGCGACGACGCCGGGCGAGGGCGCTTCGAACT
GCTCGTGCGCGGCGCGCGCTGCGCCGGCTGCATCAGCAAGATCGAAAAGGCGGTGCGCGCCCTTCCGGGCGTGGAGGCGG
CGAGGCTGAACCTGACGACCGGCAAGCTGTCGGTCGAGCTGGCCGGCAAGATCTCTGATCCGGGGCGCGTGCTGGAAACC
GTCGAGGACCTCGGCTATCGCGCCTGCCTGTTCGACCCGGCCGAAGCCGAGGCGGCGCAGGACAAGGAGGGCAAGGAACT
GGCCCTCGCTCTCGGCGTCGCGGGTTTCGGCGCCGGCAACGTCATGATGTTCACCGTGCCGGCCTGGGCGGGCCTGTTCG
GACAGGAACTGACCCCTGCCACGCTGACCCTGATGTACTGGATGGCGGCGATCGTCGCCACGCCCTGCGCACTGTTCGCC
GGCCGCCCGTTCTTCCGCTCGGCCTGGGCCTCGCTGCGGCGCGGCAAGGCCAATATGGACGTGCCGATCTCGATCGGGGT
GATCCTGACCCTGATCGTCAGCTTCTCCGAGACCCTTCTGGGCGGGAAGCATGCCTATTTCGACGCGGCGGTGACGCTGC
TGTTCCTGCTGCTGATCGGCCGCTACCTGGACCACCGTCTGCGGGCCGGAGCGCGTTCGGCGGCGCGGGATCTGCTGGCG
CTGCAGGCGCCGGTCGCCAGGCGCCTGACCGATGGCGTCGAGCAAGGCGTTCCCGTCGCGGACATCCGCGTGGGCGACCT
GTTGGCGGTCGCGCCCGGCGAGCGCATCCCGGTCGACGGCTTGGTCGAGCAAGGCGCTTCCGAACTGGACAACGCCCTGA
TCACCGGCGAGACGGCGCTGGCGCCCGTCGCCGCCGGGGCCCGACTGCACGCCGGCGCGCTGAACCTATCGGGTCGACTG
GTTATGCGGGCCTCCGCGCGCAGCGAGGACTCCACGCTCGCCGCCATCGCCCGACTGATGGAAGCCGGCGCCCAGGCGCG
CTCGACCTATGTGCGGCTCGCCGACAAGGCCGCCGCGCTCTATGTCCCCGTAGTCCACACGGCGGCGGCGCTGACCTTCG
TGGGCGGTTGGGCGCTGGGGCTGGGGCCGCGCGAGGCGCTGCTCCGCGCCGCAGCGGTGCTGATCGTGACTTGCCCGTGC
GCCCTGGGCCTGGCGGTGCCGGCGGTCCAGATCGCGGCCAGCAGCCGGCTGTTTCGCAGAGGCGTTCTGGTCAAGTCCGG
GGCGGCTCTGGAGCGTCTGGCCGAGGCCGATCACGTTGTCTTCGACAAGACCGGGGTGCTGACGCAAGGGCGTCCGGCGC
TGATCGACGCGCCCGCCCATCTGGTGGCGCAGGCCGCGCCGCTGGCCCGCGCCTCGCGGCACCCGCTGGCGCGCGCCCTG
GCGGCCGAAGCCGGCGCGGGTCCTGTGGCGCAGGACTGCGTCGAGACGGCTGGGCAGGGCGTGGAGGGCGTGATCGACGG
TCGCCGCGCTCGCCTGGGGCGCGCCGCCTTTGTCGGCGTCCAGGCGGGCGATGTTCGGGAGACCGAGCTGTGGTTCGGCT
TCGAGAACAACGTGAAGATCCGCTTCGCTTTCGAGGACCGTCCAAGAGCCGACGCCCGGGATACGATCACCAAGCTTCGG
GCGCTGGGCCTGTCTGTTGAGATCCTGTCGGGCGACGTCGAGGGTCCGGTCCGCGACGTGGCGCGCGAGGTCGGGGTCAG
CGATTGGCGCGCGGGCCTCACGCCCGTCGAGAAGGCGGCGGCGATCGATGCGTTGAAGGCGCAGGGCCGAAAGGTGCTGA
TGGTGGGAGATGGCCTCAACGACGCCGCCGCCCTGTCCAAGGCGCACGCCTCGATGGCGCCAGGCGCGGCGGTGGATGCG
GCCCAGAACGCCGCCGATCTCGTCTTTACCGGTGACGAATTGGCGGCGGTCGTCGAGTCGATCGAGACCGCCCGGGAGGC
GCGCCGACGGGCGCTGGAGAACTTCGGTTTCTCGGCGCTCTATAATCTCGTCGCCACGCCGGCGGCGATGTTCGGACTGG
TCAATCCGTTCGTGGCGGCCCTGGCGATGTCGGGCTCGTCGATTGTGGTGTTGCTCAACGCGGCGCGTCCGCGTCTTCGG
AGTTTCAACCGATGA

Upstream 100 bases:

>100_bases
GCTATCGGGCCACGGCCCGTCTGGGCGGCGCCTGGGATCTGCGCGCGACGGCGCGCGACGCCCAGAACGCGTTCGAGCTG
GAAGCCCGCCTGGTGTCGCA

Downstream 100 bases:

>100_bases
ACATCGTCCTTTTCCTGGCGCCGGCCTCGATCGGTCTGGGCGCACTGGGCTTGCTGGCCTTTTTCTGGACGATGAAGGCG
GGTCAGTACGATGACCCCAA

Product: cation transporter E1-E2 family ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 724; Mature: 723

Protein sequence:

>724_residues
MSHSLALHRDLEAFVRRDDAGRGRFELLVRGARCAGCISKIEKAVRALPGVEAARLNLTTGKLSVELAGKISDPGRVLET
VEDLGYRACLFDPAEAEAAQDKEGKELALALGVAGFGAGNVMMFTVPAWAGLFGQELTPATLTLMYWMAAIVATPCALFA
GRPFFRSAWASLRRGKANMDVPISIGVILTLIVSFSETLLGGKHAYFDAAVTLLFLLLIGRYLDHRLRAGARSAARDLLA
LQAPVARRLTDGVEQGVPVADIRVGDLLAVAPGERIPVDGLVEQGASELDNALITGETALAPVAAGARLHAGALNLSGRL
VMRASARSEDSTLAAIARLMEAGAQARSTYVRLADKAAALYVPVVHTAAALTFVGGWALGLGPREALLRAAAVLIVTCPC
ALGLAVPAVQIAASSRLFRRGVLVKSGAALERLAEADHVVFDKTGVLTQGRPALIDAPAHLVAQAAPLARASRHPLARAL
AAEAGAGPVAQDCVETAGQGVEGVIDGRRARLGRAAFVGVQAGDVRETELWFGFENNVKIRFAFEDRPRADARDTITKLR
ALGLSVEILSGDVEGPVRDVAREVGVSDWRAGLTPVEKAAAIDALKAQGRKVLMVGDGLNDAAALSKAHASMAPGAAVDA
AQNAADLVFTGDELAAVVESIETAREARRRALENFGFSALYNLVATPAAMFGLVNPFVAALAMSGSSIVVLLNAARPRLR
SFNR

Sequences:

>Translated_724_residues
MSHSLALHRDLEAFVRRDDAGRGRFELLVRGARCAGCISKIEKAVRALPGVEAARLNLTTGKLSVELAGKISDPGRVLET
VEDLGYRACLFDPAEAEAAQDKEGKELALALGVAGFGAGNVMMFTVPAWAGLFGQELTPATLTLMYWMAAIVATPCALFA
GRPFFRSAWASLRRGKANMDVPISIGVILTLIVSFSETLLGGKHAYFDAAVTLLFLLLIGRYLDHRLRAGARSAARDLLA
LQAPVARRLTDGVEQGVPVADIRVGDLLAVAPGERIPVDGLVEQGASELDNALITGETALAPVAAGARLHAGALNLSGRL
VMRASARSEDSTLAAIARLMEAGAQARSTYVRLADKAAALYVPVVHTAAALTFVGGWALGLGPREALLRAAAVLIVTCPC
ALGLAVPAVQIAASSRLFRRGVLVKSGAALERLAEADHVVFDKTGVLTQGRPALIDAPAHLVAQAAPLARASRHPLARAL
AAEAGAGPVAQDCVETAGQGVEGVIDGRRARLGRAAFVGVQAGDVRETELWFGFENNVKIRFAFEDRPRADARDTITKLR
ALGLSVEILSGDVEGPVRDVAREVGVSDWRAGLTPVEKAAAIDALKAQGRKVLMVGDGLNDAAALSKAHASMAPGAAVDA
AQNAADLVFTGDELAAVVESIETAREARRRALENFGFSALYNLVATPAAMFGLVNPFVAALAMSGSSIVVLLNAARPRLR
SFNR
>Mature_723_residues
SHSLALHRDLEAFVRRDDAGRGRFELLVRGARCAGCISKIEKAVRALPGVEAARLNLTTGKLSVELAGKISDPGRVLETV
EDLGYRACLFDPAEAEAAQDKEGKELALALGVAGFGAGNVMMFTVPAWAGLFGQELTPATLTLMYWMAAIVATPCALFAG
RPFFRSAWASLRRGKANMDVPISIGVILTLIVSFSETLLGGKHAYFDAAVTLLFLLLIGRYLDHRLRAGARSAARDLLAL
QAPVARRLTDGVEQGVPVADIRVGDLLAVAPGERIPVDGLVEQGASELDNALITGETALAPVAAGARLHAGALNLSGRLV
MRASARSEDSTLAAIARLMEAGAQARSTYVRLADKAAALYVPVVHTAAALTFVGGWALGLGPREALLRAAAVLIVTCPCA
LGLAVPAVQIAASSRLFRRGVLVKSGAALERLAEADHVVFDKTGVLTQGRPALIDAPAHLVAQAAPLARASRHPLARALA
AEAGAGPVAQDCVETAGQGVEGVIDGRRARLGRAAFVGVQAGDVRETELWFGFENNVKIRFAFEDRPRADARDTITKLRA
LGLSVEILSGDVEGPVRDVAREVGVSDWRAGLTPVEKAAAIDALKAQGRKVLMVGDGLNDAAALSKAHASMAPGAAVDAA
QNAADLVFTGDELAAVVESIETAREARRRALENFGFSALYNLVATPAAMFGLVNPFVAALAMSGSSIVVLLNAARPRLRS
FNR

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=488, Percent_Identity=28.6885245901639, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI115529486, Length=529, Percent_Identity=27.4102079395085, Blast_Score=194, Evalue=3e-49,
Organism=Homo sapiens, GI55743073, Length=221, Percent_Identity=31.2217194570136, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1786691, Length=736, Percent_Identity=31.7934782608696, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI1789879, Length=726, Percent_Identity=30.0275482093664, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1786914, Length=547, Percent_Identity=27.4223034734918, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17556548, Length=545, Percent_Identity=28.256880733945, Blast_Score=182, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6319772, Length=616, Percent_Identity=30.1948051948052, Blast_Score=233, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6320475, Length=635, Percent_Identity=26.1417322834646, Blast_Score=200, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6325221, Length=154, Percent_Identity=31.1688311688312, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321430, Length=154, Percent_Identity=31.1688311688312, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI221329854, Length=521, Percent_Identity=29.1746641074856, Blast_Score=190, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 75800; Mature: 75669

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSLALHRDLEAFVRRDDAGRGRFELLVRGARCAGCISKIEKAVRALPGVEAARLNLTT
CCCCHHHHHHHHHHHHCCCCCCCCEEHEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GKLSVELAGKISDPGRVLETVEDLGYRACLFDPAEAEAAQDKEGKELALALGVAGFGAGN
CEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCEEEEEEECCCCCCCC
VMMFTVPAWAGLFGQELTPATLTLMYWMAAIVATPCALFAGRPFFRSAWASLRRGKANMD
EEEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
VPISIGVILTLIVSFSETLLGGKHAYFDAAVTLLFLLLIGRYLDHRLRAGARSAARDLLA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LQAPVARRLTDGVEQGVPVADIRVGDLLAVAPGERIPVDGLVEQGASELDNALITGETAL
HHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCCHHHHHHHCHHHHCCCEECCCHHH
APVAAGARLHAGALNLSGRLVMRASARSEDSTLAAIARLMEAGAQARSTYVRLADKAAAL
HHHHCCCEEECCEECCCCEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHE
YVPVVHTAAALTFVGGWALGLGPREALLRAAAVLIVTCPCALGLAVPAVQIAASSRLFRR
EHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVLVKSGAALERLAEADHVVFDKTGVLTQGRPALIDAPAHLVAQAAPLARASRHPLARAL
CCCEECCHHHHHHHHCCCEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCHHHHHH
AAEAGAGPVAQDCVETAGQGVEGVIDGRRARLGRAAFVGVQAGDVRETELWFGFENNVKI
HHHCCCCCHHHHHHHHHCCCCHHHHCCHHHHCCCCEEEECCCCCCCCCEEEEEECCCEEE
RFAFEDRPRADARDTITKLRALGLSVEILSGDVEGPVRDVAREVGVSDWRAGLTPVEKAA
EEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCHHHHCCCCHHHHHH
AIDALKAQGRKVLMVGDGLNDAAALSKAHASMAPGAAVDAAQNAADLVFTGDELAAVVES
HHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECHHHHHHHHH
IETAREARRRALENFGFSALYNLVATPAAMFGLVNPFVAALAMSGSSIVVLLNAARPRLR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
SFNR
CCCC
>Mature Secondary Structure 
SHSLALHRDLEAFVRRDDAGRGRFELLVRGARCAGCISKIEKAVRALPGVEAARLNLTT
CCCHHHHHHHHHHHHCCCCCCCCEEHEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GKLSVELAGKISDPGRVLETVEDLGYRACLFDPAEAEAAQDKEGKELALALGVAGFGAGN
CEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCEEEEEEECCCCCCCC
VMMFTVPAWAGLFGQELTPATLTLMYWMAAIVATPCALFAGRPFFRSAWASLRRGKANMD
EEEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
VPISIGVILTLIVSFSETLLGGKHAYFDAAVTLLFLLLIGRYLDHRLRAGARSAARDLLA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LQAPVARRLTDGVEQGVPVADIRVGDLLAVAPGERIPVDGLVEQGASELDNALITGETAL
HHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCCHHHHHHHCHHHHCCCEECCCHHH
APVAAGARLHAGALNLSGRLVMRASARSEDSTLAAIARLMEAGAQARSTYVRLADKAAAL
HHHHCCCEEECCEECCCCEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHE
YVPVVHTAAALTFVGGWALGLGPREALLRAAAVLIVTCPCALGLAVPAVQIAASSRLFRR
EHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVLVKSGAALERLAEADHVVFDKTGVLTQGRPALIDAPAHLVAQAAPLARASRHPLARAL
CCCEECCHHHHHHHHCCCEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCHHHHHH
AAEAGAGPVAQDCVETAGQGVEGVIDGRRARLGRAAFVGVQAGDVRETELWFGFENNVKI
HHHCCCCCHHHHHHHHHCCCCHHHHCCHHHHCCCCEEEECCCCCCCCCEEEEEECCCEEE
RFAFEDRPRADARDTITKLRALGLSVEILSGDVEGPVRDVAREVGVSDWRAGLTPVEKAA
EEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCHHHHCCCCHHHHHH
AIDALKAQGRKVLMVGDGLNDAAALSKAHASMAPGAAVDAAQNAADLVFTGDELAAVVES
HHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECHHHHHHHHH
IETAREARRRALENFGFSALYNLVATPAAMFGLVNPFVAALAMSGSSIVVLLNAARPRLR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
SFNR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661920; 12597275 [H]