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The map label for this gene is glyA

Identifier: 16125606

GI number: 16125606

Start: 1510744

End: 1512033

Strand: Direct

Name: glyA

Synonym: CC_1357

Alternate gene names: 16125606

Gene position: 1510744-1512033 (Clockwise)

Preceding gene: 16125604

Following gene: 16125607

Centisome position: 37.61

GC content: 65.89

Gene sequence:

>1290_bases
GTGATGACCCAAACCGACCTCAGCGCCTTTTTCGGCGCGGACCTCGCCACCGCGGACCGCGACATCTTCGATCGCATCGG
TCGCGAGCTGGGTCGCCAGCAGAACCAGATCGAGCTGATCGCCTCGGAGAACATCGTCTCCAAGGCCGTGCTTGAGGCCC
AGGGCTCGATCCTGACCAACAAGTACGCCGAAGGCTATCCGGGCAAGCGCTACTATGGCGGCTGCGAATATGTCGACGAG
ATCGAGACGATCGCGATCGAGCGCGCCAAGGCGCTGTTCGGCGCGGGCTTCGCCAATGTTCAGCCGCACTCGGGCTCGCA
GGCCAACCAAGCGGTGTTCATGGCGCTGCTGCAGCCGGGCGACACCTTCCTGGGCATGGACCTCGCAGCCGGCGGCCATT
TGACGCACGGCAGCCCCGCCAACCAGTCGGGCAAGTGGTTCAAGCCGATTTCCTACTCGGTCCGCCAGCAGGACCAGCTG
ATCGACTATGACGGCGTCGCCGAGGTCGCCCAACGCGAGAAGCCCAAGCTGATCATCGCCGGCGGCAGCGCCTACAGCCG
CGAGATCGACTTCGCCAAGTTCCGCGAGATCGCCGACTCGATCGGCGCCTATCTGATGGTGGACATGGCGCACTACGCCG
GCCTGATCGCCGGCGGCGCCTATGCCAACCCGATCCCGCACGCCCACATCGTCACCACGACCACGCACAAGACGCTGCGC
GGCCCGCGCGGCGGCTTGGTGCTGACCAACGACGAAGCGATCATCAAGAAGGTCAATTCGGCGGTGTTCCCCGGCCTGCA
GGGCGGCCCGCTGGAGCACGTCATCGCGGCCAAGGCCGTCGCTTTCGGTGAAGCGCTGCAGCCTTCGTTCAAGGACTATG
CGCGACAAGTTGTCGCGAACGCGCGGGCCCTGGCCGAGGCGCTGCTGAAGTCGGGCGTGAATATCGTCTCGGGCGGCACC
GACAGCCACCTGATGCTGGTCGACCTGCGCCCGAAGGGCGTGACCGGCCGCGACGCCGAGCACAGCCTCGAGCGCGCCTA
CATGACCTGCAACAAGAACGGCGTGCCGTTCGACACTGCGCCGTTCACGATCACTTCGGGCATTCGCCTGGGCACGCCGG
CCGGTACGACGCGCGGCTTCAAGGAAGCCGAGTTCACGCGCGTTGGCGAGCTGATCGGCGAGGTCGTCAACGGCCTGGCG
GTCAATGGTCCGGAAGGCAACGCCGCCGTCGAGGCCAAGGTTCGCGAAGAAGTGCTGGCCTTGACGGGTCGCTTCCCGAT
CTACAACTAA

Upstream 100 bases:

>100_bases
GTCGACAACGCGCAACATCTCGAGTGGTTCCGAGATTGATCGCCGCGCCTATCCCGTTAAAGAGGGCGCCTCTGCCGACT
CCCCGCAACGCCAGGAGAGC

Downstream 100 bases:

>100_bases
TAGGGTGATTGCGCCGCGGAGGGGGCTAGATCATGCGTTGCCCGTTCTGCGGTCATGCCGAAAGCCAGGTCAAGGATAGC
CGCCCGTCGGAAGACGGCGC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MMTQTDLSAFFGADLATADRDIFDRIGRELGRQQNQIELIASENIVSKAVLEAQGSILTNKYAEGYPGKRYYGGCEYVDE
IETIAIERAKALFGAGFANVQPHSGSQANQAVFMALLQPGDTFLGMDLAAGGHLTHGSPANQSGKWFKPISYSVRQQDQL
IDYDGVAEVAQREKPKLIIAGGSAYSREIDFAKFREIADSIGAYLMVDMAHYAGLIAGGAYANPIPHAHIVTTTTHKTLR
GPRGGLVLTNDEAIIKKVNSAVFPGLQGGPLEHVIAAKAVAFGEALQPSFKDYARQVVANARALAEALLKSGVNIVSGGT
DSHLMLVDLRPKGVTGRDAEHSLERAYMTCNKNGVPFDTAPFTITSGIRLGTPAGTTRGFKEAEFTRVGELIGEVVNGLA
VNGPEGNAAVEAKVREEVLALTGRFPIYN

Sequences:

>Translated_429_residues
MMTQTDLSAFFGADLATADRDIFDRIGRELGRQQNQIELIASENIVSKAVLEAQGSILTNKYAEGYPGKRYYGGCEYVDE
IETIAIERAKALFGAGFANVQPHSGSQANQAVFMALLQPGDTFLGMDLAAGGHLTHGSPANQSGKWFKPISYSVRQQDQL
IDYDGVAEVAQREKPKLIIAGGSAYSREIDFAKFREIADSIGAYLMVDMAHYAGLIAGGAYANPIPHAHIVTTTTHKTLR
GPRGGLVLTNDEAIIKKVNSAVFPGLQGGPLEHVIAAKAVAFGEALQPSFKDYARQVVANARALAEALLKSGVNIVSGGT
DSHLMLVDLRPKGVTGRDAEHSLERAYMTCNKNGVPFDTAPFTITSGIRLGTPAGTTRGFKEAEFTRVGELIGEVVNGLA
VNGPEGNAAVEAKVREEVLALTGRFPIYN
>Mature_429_residues
MMTQTDLSAFFGADLATADRDIFDRIGRELGRQQNQIELIASENIVSKAVLEAQGSILTNKYAEGYPGKRYYGGCEYVDE
IETIAIERAKALFGAGFANVQPHSGSQANQAVFMALLQPGDTFLGMDLAAGGHLTHGSPANQSGKWFKPISYSVRQQDQL
IDYDGVAEVAQREKPKLIIAGGSAYSREIDFAKFREIADSIGAYLMVDMAHYAGLIAGGAYANPIPHAHIVTTTTHKTLR
GPRGGLVLTNDEAIIKKVNSAVFPGLQGGPLEHVIAAKAVAFGEALQPSFKDYARQVVANARALAEALLKSGVNIVSGGT
DSHLMLVDLRPKGVTGRDAEHSLERAYMTCNKNGVPFDTAPFTITSGIRLGTPAGTTRGFKEAEFTRVGELIGEVVNGLA
VNGPEGNAAVEAKVREEVLALTGRFPIYN

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=412, Percent_Identity=49.7572815533981, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI261862352, Length=412, Percent_Identity=49.7572815533981, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI261862350, Length=412, Percent_Identity=49.7572815533981, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI261862348, Length=412, Percent_Identity=49.7572815533981, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI261862346, Length=412, Percent_Identity=48.7864077669903, Blast_Score=357, Evalue=9e-99,
Organism=Homo sapiens, GI22547186, Length=457, Percent_Identity=45.9518599562363, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI22547189, Length=440, Percent_Identity=43.4090909090909, Blast_Score=306, Evalue=3e-83,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=57.5539568345324, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=48.5, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=48.5, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319739, Length=406, Percent_Identity=44.0886699507389, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6323087, Length=408, Percent_Identity=43.3823529411765, Blast_Score=322, Evalue=8e-89,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=47.75, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=47.75, Blast_Score=361, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_CAUCR (Q9A8J6)

Other databases:

- EMBL:   AE005673
- PIR:   F87417
- RefSeq:   NP_420170.1
- ProteinModelPortal:   Q9A8J6
- SMR:   Q9A8J6
- GeneID:   941694
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_1357
- NMPDR:   fig|190650.1.peg.1350
- TIGR:   CC_1357
- HOGENOM:   HBG301263
- OMA:   NDEAIIK
- ProtClustDB:   PRK00011
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45827; Mature: 45827

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTQTDLSAFFGADLATADRDIFDRIGRELGRQQNQIELIASENIVSKAVLEAQGSILTN
CCCCHHHHHHHCCCHHHCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHH
KYAEGYPGKRYYGGCEYVDEIETIAIERAKALFGAGFANVQPHSGSQANQAVFMALLQPG
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEECCC
DTFLGMDLAAGGHLTHGSPANQSGKWFKPISYSVRQQDQLIDYDGVAEVAQREKPKLIIA
CCEEEEEECCCCCCCCCCCCCCCCCEECCCCHHHHHHCCCCCCCCHHHHHHCCCCEEEEE
GGSAYSREIDFAKFREIADSIGAYLMVDMAHYAGLIAGGAYANPIPHAHIVTTTTHKTLR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHC
GPRGGLVLTNDEAIIKKVNSAVFPGLQGGPLEHVIAAKAVAFGEALQPSFKDYARQVVAN
CCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ARALAEALLKSGVNIVSGGTDSHLMLVDLRPKGVTGRDAEHSLERAYMTCNKNGVPFDTA
HHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
PFTITSGIRLGTPAGTTRGFKEAEFTRVGELIGEVVNGLAVNGPEGNAAVEAKVREEVLA
CEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHH
LTGRFPIYN
HHCCCCCCC
>Mature Secondary Structure
MMTQTDLSAFFGADLATADRDIFDRIGRELGRQQNQIELIASENIVSKAVLEAQGSILTN
CCCCHHHHHHHCCCHHHCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHH
KYAEGYPGKRYYGGCEYVDEIETIAIERAKALFGAGFANVQPHSGSQANQAVFMALLQPG
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEECCC
DTFLGMDLAAGGHLTHGSPANQSGKWFKPISYSVRQQDQLIDYDGVAEVAQREKPKLIIA
CCEEEEEECCCCCCCCCCCCCCCCCEECCCCHHHHHHCCCCCCCCHHHHHHCCCCEEEEE
GGSAYSREIDFAKFREIADSIGAYLMVDMAHYAGLIAGGAYANPIPHAHIVTTTTHKTLR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHC
GPRGGLVLTNDEAIIKKVNSAVFPGLQGGPLEHVIAAKAVAFGEALQPSFKDYARQVVAN
CCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ARALAEALLKSGVNIVSGGTDSHLMLVDLRPKGVTGRDAEHSLERAYMTCNKNGVPFDTA
HHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
PFTITSGIRLGTPAGTTRGFKEAEFTRVGELIGEVVNGLAVNGPEGNAAVEAKVREEVLA
CEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHH
LTGRFPIYN
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647