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The map label for this gene is ispE

Identifier: 16125585

GI number: 16125585

Start: 1490969

End: 1491856

Strand: Direct

Name: ispE

Synonym: CC_1336

Alternate gene names: 16125585

Gene position: 1490969-1491856 (Clockwise)

Preceding gene: 16125584

Following gene: 16125586

Centisome position: 37.12

GC content: 70.5

Gene sequence:

>888_bases
TTGAGTCACGAGATCATGCGGCTGTCAGCGTTCGCCCCGGCCAAGGTCAATCTGTTCCTGCATGTCGGCGGGCCGGACGG
GGAGGGCTATCATCCGATCTCCAGCCTGATGGTGTTCGCCGATGTCGGCGACCGGGTGAACCTCCAGCCCGCCGACGCGC
CGGCGTTCGAGACGTCCGGGCCGTTCGGGGATCAGATTCCCGCCGGCGGCGACAATCTGGTGGTCCGCGCAGGCCAGGCT
TTCCACAGGCGGCTCGGCGGTCCGGTTCCGCCTTATCGGCTGATCCTGGAGAAGCACTTGCCGATCGCTGCGGGCCTGGG
CGGGGGCTCCAGCGACGCGGGTGCGGCGCTCAAGCTGTTGCGTGACGCCCTGGCCCCGGCGCTTTCCGATGACGATCTGG
AGGCGCTGGCTGCGAGCCTTGGCGCGGACGGCGCGGCCTGTCTGCGCGCGCGCGCCCTTATCGCAGAAGGGCGAGGCGAG
CGGCTGTCGCCGGCGCCGCGGTTGCCCGAGTTGAACGCAGTGCTGGTCAATCCCGGCGCGCCGTCGCCGACCGGCGCGGT
CTATCGCGCCTATGACGCGGGCGTGCACCCAGACGGCGCCGCCATGCCCCCCATGCCCGACCATCTGGAGAGCGCTGAGG
AGGCCGCCGCCTGGTTGGCCTTTGCGACACGCAACGACCTGGAGGCGCCGGCCGTGAGGCTTGAGCCGCACATCGGCGAG
GTGCTGGACGTGCTGCGGGGCGAGCCGGAAAGTCTGCTGGTCCGCATGTCGGGTTCGGGTGCGACCTGTTTCGCGCTCTG
CGCCAGCGATATCGAGGCCGAAGGTCTGGCCGAGCGCCTGGAGACGATGCGTCCGGACTGGTGGGTGCGGCGCTGCCGCC
TGAGCTGA

Upstream 100 bases:

>100_bases
ACTCCGAGGACAAGCTGAAGAACGGCCTGGGCCTCTCGACGCCGGCGACCAAGTCGACGATCGCCCACAACTGAGCTATC
GGCTTTCGCCTGTCCTAGCC

Downstream 100 bases:

>100_bases
GGAGACGATGATGAAGCGCCGTGACGTCCTGGCCGCCTTCGGCGTTTTCGCGGTCCCGCCGGGTCTGGCCTCAGCGCAGA
TTTTGAGCCTGCCCGCGACA

Product: GHMP family kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSHEIMRLSAFAPAKVNLFLHVGGPDGEGYHPISSLMVFADVGDRVNLQPADAPAFETSGPFGDQIPAGGDNLVVRAGQA
FHRRLGGPVPPYRLILEKHLPIAAGLGGGSSDAGAALKLLRDALAPALSDDDLEALAASLGADGAACLRARALIAEGRGE
RLSPAPRLPELNAVLVNPGAPSPTGAVYRAYDAGVHPDGAAMPPMPDHLESAEEAAAWLAFATRNDLEAPAVRLEPHIGE
VLDVLRGEPESLLVRMSGSGATCFALCASDIEAEGLAERLETMRPDWWVRRCRLS

Sequences:

>Translated_295_residues
MSHEIMRLSAFAPAKVNLFLHVGGPDGEGYHPISSLMVFADVGDRVNLQPADAPAFETSGPFGDQIPAGGDNLVVRAGQA
FHRRLGGPVPPYRLILEKHLPIAAGLGGGSSDAGAALKLLRDALAPALSDDDLEALAASLGADGAACLRARALIAEGRGE
RLSPAPRLPELNAVLVNPGAPSPTGAVYRAYDAGVHPDGAAMPPMPDHLESAEEAAAWLAFATRNDLEAPAVRLEPHIGE
VLDVLRGEPESLLVRMSGSGATCFALCASDIEAEGLAERLETMRPDWWVRRCRLS
>Mature_294_residues
SHEIMRLSAFAPAKVNLFLHVGGPDGEGYHPISSLMVFADVGDRVNLQPADAPAFETSGPFGDQIPAGGDNLVVRAGQAF
HRRLGGPVPPYRLILEKHLPIAAGLGGGSSDAGAALKLLRDALAPALSDDDLEALAASLGADGAACLRARALIAEGRGER
LSPAPRLPELNAVLVNPGAPSPTGAVYRAYDAGVHPDGAAMPPMPDHLESAEEAAAWLAFATRNDLEAPAVRLEPHIGEV
LDVLRGEPESLLVRMSGSGATCFALCASDIEAEGLAERLETMRPDWWVRRCRLS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=291, Percent_Identity=30.2405498281787, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CAUCR (Q9A8L7)

Other databases:

- EMBL:   AE005673
- PIR:   A87415
- RefSeq:   NP_420149.1
- ProteinModelPortal:   Q9A8L7
- SMR:   Q9A8L7
- GeneID:   942489
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_1336
- NMPDR:   fig|190650.1.peg.1329
- TIGR:   CC_1336
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- ProtClustDB:   PRK14608
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30905; Mature: 30774

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: ACT_SITE 15-15 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHEIMRLSAFAPAKVNLFLHVGGPDGEGYHPISSLMVFADVGDRVNLQPADAPAFETSG
CCCCEEEEECCCCEEEEEEEEECCCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCC
PFGDQIPAGGDNLVVRAGQAFHRRLGGPVPPYRLILEKHLPIAAGLGGGSSDAGAALKLL
CCCCCCCCCCCCEEEECCHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
RDALAPALSDDDLEALAASLGADGAACLRARALIAEGRGERLSPAPRLPELNAVLVNPGA
HHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCC
PSPTGAVYRAYDAGVHPDGAAMPPMPDHLESAEEAAAWLAFATRNDLEAPAVRLEPHIGE
CCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHH
VLDVLRGEPESLLVRMSGSGATCFALCASDIEAEGLAERLETMRPDWWVRRCRLS
HHHHHCCCCCCEEEEECCCCCEEEEHHHHCCCHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
SHEIMRLSAFAPAKVNLFLHVGGPDGEGYHPISSLMVFADVGDRVNLQPADAPAFETSG
CCCEEEEECCCCEEEEEEEEECCCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCC
PFGDQIPAGGDNLVVRAGQAFHRRLGGPVPPYRLILEKHLPIAAGLGGGSSDAGAALKLL
CCCCCCCCCCCCEEEECCHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
RDALAPALSDDDLEALAASLGADGAACLRARALIAEGRGERLSPAPRLPELNAVLVNPGA
HHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCC
PSPTGAVYRAYDAGVHPDGAAMPPMPDHLESAEEAAAWLAFATRNDLEAPAVRLEPHIGE
CCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHH
VLDVLRGEPESLLVRMSGSGATCFALCASDIEAEGLAERLETMRPDWWVRRCRLS
HHHHHCCCCCCEEEEECCCCCEEEEHHHHCCCHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647