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The map label for this gene is rotA [H]

Identifier: 16125586

GI number: 16125586

Start: 1491864

End: 1492502

Strand: Direct

Name: rotA [H]

Synonym: CC_1337

Alternate gene names: 16125586

Gene position: 1491864-1492502 (Clockwise)

Preceding gene: 16125585

Following gene: 16125590

Centisome position: 37.14

GC content: 66.35

Gene sequence:

>639_bases
ATGATGAAGCGCCGTGACGTCCTGGCCGCCTTCGGCGTTTTCGCGGTCCCGCCGGGTCTGGCCTCAGCGCAGATTTTGAG
CCTGCCCGCGACACCGATCGTGCCGGGCGCGGGCGATGTTCTGGTCCGCCTCGACACCGGTCTTGGTCCGATCATCATCG
CGCTGAAGGCAAGACAGGCGCCGCTGACCGTGGCGAACTTCCTCCGGTATGTGGACGAGAAGCGCTATGACGGTTCGAGC
TTCTGGCGCGCAGCCAAGGCGCCCAGCGCGGTGGATTACGGGTTGATCCAAGGTGGCCTGCAGGGGGATCCGAAGAGGCT
GCTGCCGCCCATCGCGCATGAGCCGACGACCCAGACGGGGCTCCGGCATGTCGACGGGACGTTGTCGCTGGCGCGCAAGG
CGCCGGGCACCGGCGATAGCGACTTCTTCATCTGCGTGGGTGACGCGCCCTATCTCGACGCCAATCCGGCTGGGGAGGGG
GACAATCTCGGCTTCGCGGCCTTTGGGCAGGTGGTGCAGGGCATGGAGATCGTCCGCAAGATCCTGAACCTGCCGACGCC
GGGCAAGGCGATCAATCCGGTCATGGAGGGTCAGATGCTCGATCCGGTCGTGCCGATCGTGACGGCGCGACGGATCTAG

Upstream 100 bases:

>100_bases
CTCTGCGCCAGCGATATCGAGGCCGAAGGTCTGGCCGAGCGCCTGGAGACGATGCGTCCGGACTGGTGGGTGCGGCGCTG
CCGCCTGAGCTGAGGAGACG

Downstream 100 bases:

>100_bases
GCAGTCGGCATGGCCAAAAAGAGAAAGGGCGCGGAGGTTTCCCTCCGCGCCCTTCGACTTTGAGCCTGGCCCCAGCCTTA
GCTGTGGTAGGCGCGTTCGC

Product: peptidyl-prolyl cis-trans isomerase A

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MMKRRDVLAAFGVFAVPPGLASAQILSLPATPIVPGAGDVLVRLDTGLGPIIIALKARQAPLTVANFLRYVDEKRYDGSS
FWRAAKAPSAVDYGLIQGGLQGDPKRLLPPIAHEPTTQTGLRHVDGTLSLARKAPGTGDSDFFICVGDAPYLDANPAGEG
DNLGFAAFGQVVQGMEIVRKILNLPTPGKAINPVMEGQMLDPVVPIVTARRI

Sequences:

>Translated_212_residues
MMKRRDVLAAFGVFAVPPGLASAQILSLPATPIVPGAGDVLVRLDTGLGPIIIALKARQAPLTVANFLRYVDEKRYDGSS
FWRAAKAPSAVDYGLIQGGLQGDPKRLLPPIAHEPTTQTGLRHVDGTLSLARKAPGTGDSDFFICVGDAPYLDANPAGEG
DNLGFAAFGQVVQGMEIVRKILNLPTPGKAINPVMEGQMLDPVVPIVTARRI
>Mature_212_residues
MMKRRDVLAAFGVFAVPPGLASAQILSLPATPIVPGAGDVLVRLDTGLGPIIIALKARQAPLTVANFLRYVDEKRYDGSS
FWRAAKAPSAVDYGLIQGGLQGDPKRLLPPIAHEPTTQTGLRHVDGTLSLARKAPGTGDSDFFICVGDAPYLDANPAGEG
DNLGFAAFGQVVQGMEIVRKILNLPTPGKAINPVMEGQMLDPVVPIVTARRI

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789763, Length=153, Percent_Identity=37.9084967320261, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786736, Length=151, Percent_Identity=35.0993377483444, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 22373; Mature: 22373

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRRDVLAAFGVFAVPPGLASAQILSLPATPIVPGAGDVLVRLDTGLGPIIIALKARQA
CCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEECCCC
PLTVANFLRYVDEKRYDGSSFWRAAKAPSAVDYGLIQGGLQGDPKRLLPPIAHEPTTQTG
CHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCHHH
LRHVDGTLSLARKAPGTGDSDFFICVGDAPYLDANPAGEGDNLGFAAFGQVVQGMEIVRK
HHHHCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ILNLPTPGKAINPVMEGQMLDPVVPIVTARRI
HHCCCCCCCCCCHHHCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MMKRRDVLAAFGVFAVPPGLASAQILSLPATPIVPGAGDVLVRLDTGLGPIIIALKARQA
CCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEECCCC
PLTVANFLRYVDEKRYDGSSFWRAAKAPSAVDYGLIQGGLQGDPKRLLPPIAHEPTTQTG
CHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCHHH
LRHVDGTLSLARKAPGTGDSDFFICVGDAPYLDANPAGEGDNLGFAAFGQVVQGMEIVRK
HHHHCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ILNLPTPGKAINPVMEGQMLDPVVPIVTARRI
HHCCCCCCCCCCHHHCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]