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The map label for this gene is rutB [H]

Identifier: 16125422

GI number: 16125422

Start: 1329069

End: 1329698

Strand: Direct

Name: rutB [H]

Synonym: CC_1170

Alternate gene names: 16125422

Gene position: 1329069-1329698 (Clockwise)

Preceding gene: 16125421

Following gene: 16125424

Centisome position: 33.09

GC content: 69.52

Gene sequence:

>630_bases
GTGAGCTTGCCGGCCTGGATCGCGCCGCAGCGCACGGCGTTGATGATTGTGGACATGCAGGTCGATTTCGCCGCGCCCGA
GGGGCTGTCCGCCCAGTGGGGCATGGATCTGTCGGCCGTGCCGGGCGCCCTTGCGGCGGCCCAGCGTCTGGCGGAGGCCG
CGCGCGCCGCCGGGACGCCGGTGGTGTTCGTGGGTCTGTTCACGACGCCCGAAACAGACTCTGACGTGTGGGGCGAGCGA
CTGCGGCGGCAAGGCCATACGGCTGAAGACAGCCCCGGGCTGTGTCGCGCCGGCGCGCCCGGCAGCGCCTTCGTCGGGCC
TCAGCCTCAAGCCGACGACCTCGTCTTCCGCAAGACGCGCTACAGCCCGTTCTGGGACACCGATATCGCCGCCCGGCTGC
GGTCCATGGGCGTCGACACGCTTGTGCTGGCGGGGCTGACCACGGAATGCTGCATCGACAGCACCGCGCGCGACGCCTTC
AATCACGACTTCCACGTGTTCGTGTCGATCGACGCCTGCGCCGCCTATGAGCCCGAACTGCATGCGGCGGCCTTGCGGAT
GCTGGAGCTCAACACGGCGATCCTGACCGACAGTGACAGCGTCGTCGTGGCTTGGCGCTCGGCCGTCTAG

Upstream 100 bases:

>100_bases
CGTGCGAACTGGACGGCCTGATGCTGATCTTTCCGGACTATGTCGAAGGGCTTTCCATGTTCGGAGCGGAGATCCTGCCG
GGCCTGCGGGCGGTGTTCTC

Downstream 100 bases:

>100_bases
CCGTCCTGTCGCGGCGCTGCGGCGCCTTGCATCGCCGCCCGCAGGTCTTCTTCCAGCATATAGAGCCGGTCCTGGCCCCA
GTGCGCTTGGTGACCCGTGA

Product: isochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSLPAWIAPQRTALMIVDMQVDFAAPEGLSAQWGMDLSAVPGALAAAQRLAEAARAAGTPVVFVGLFTTPETDSDVWGER
LRRQGHTAEDSPGLCRAGAPGSAFVGPQPQADDLVFRKTRYSPFWDTDIAARLRSMGVDTLVLAGLTTECCIDSTARDAF
NHDFHVFVSIDACAAYEPELHAAALRMLELNTAILTDSDSVVVAWRSAV

Sequences:

>Translated_209_residues
MSLPAWIAPQRTALMIVDMQVDFAAPEGLSAQWGMDLSAVPGALAAAQRLAEAARAAGTPVVFVGLFTTPETDSDVWGER
LRRQGHTAEDSPGLCRAGAPGSAFVGPQPQADDLVFRKTRYSPFWDTDIAARLRSMGVDTLVLAGLTTECCIDSTARDAF
NHDFHVFVSIDACAAYEPELHAAALRMLELNTAILTDSDSVVVAWRSAV
>Mature_208_residues
SLPAWIAPQRTALMIVDMQVDFAAPEGLSAQWGMDLSAVPGALAAAQRLAEAARAAGTPVVFVGLFTTPETDSDVWGERL
RRQGHTAEDSPGLCRAGAPGSAFVGPQPQADDLVFRKTRYSPFWDTDIAARLRSMGVDTLVLAGLTTECCIDSTARDAFN
HDFHVFVSIDACAAYEPELHAAALRMLELNTAILTDSDSVVVAWRSAV

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=213, Percent_Identity=29.5774647887324, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87081992, Length=190, Percent_Identity=28.9473684210526, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 22363; Mature: 22232

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPAWIAPQRTALMIVDMQVDFAAPEGLSAQWGMDLSAVPGALAAAQRLAEAARAAGTP
CCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHCCCCHHHCCHHHHHHHHHHHHHHHCCCC
VVFVGLFTTPETDSDVWGERLRRQGHTAEDSPGLCRAGAPGSAFVGPQPQADDLVFRKTR
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEECC
YSPFWDTDIAARLRSMGVDTLVLAGLTTECCIDSTARDAFNHDFHVFVSIDACAAYEPEL
CCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHCCHHHHHCCCCEEEEEEECCCCCCCCHH
HAAALRMLELNTAILTDSDSVVVAWRSAV
HHHHHHHHHHCEEEEECCCCEEEEEECCC
>Mature Secondary Structure 
SLPAWIAPQRTALMIVDMQVDFAAPEGLSAQWGMDLSAVPGALAAAQRLAEAARAAGTP
CCCCCCCCCCEEEEEEEEEEEECCCCCCCHHCCCCHHHCCHHHHHHHHHHHHHHHCCCC
VVFVGLFTTPETDSDVWGERLRRQGHTAEDSPGLCRAGAPGSAFVGPQPQADDLVFRKTR
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEECC
YSPFWDTDIAARLRSMGVDTLVLAGLTTECCIDSTARDAFNHDFHVFVSIDACAAYEPEL
CCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHCCHHHHHCCCCEEEEEEECCCCCCCCHH
HAAALRMLELNTAILTDSDSVVVAWRSAV
HHHHHHHHHHCEEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA