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The map label for this gene is rfbD [C]

Identifier: 16125392

GI number: 16125392

Start: 1295692

End: 1296594

Strand: Direct

Name: rfbD [C]

Synonym: CC_1140

Alternate gene names: 16125392

Gene position: 1295692-1296594 (Clockwise)

Preceding gene: 16125390

Following gene: 16125393

Centisome position: 32.26

GC content: 67.55

Gene sequence:

>903_bases
ATGACCGACAAGACGCATATTCTCGTCACCGGCGGCGCCGGACAGGTGGGGTTGGAGCTTTTGGGCGCCGCATGGCCGGA
CGGGATCGTGCTGCATGCGCCGTCCCGCGCCGCGTTGGACCTGACGAACGAGACGTCGATCCGGGCCGCCTTCGAGGCGA
GGCCCTTCGCCGCGGTGATCAATTCGGCCGCCTGGACGGCCGTGGACAAGGCCGAGAGCGAGGTCGCGGCCGCCTTCGCC
GCCAACGCCATCGGACCAGCCCTGCTGGCTGAGGTGACGCGGGCGAGGGGCGTTCCGCTGATTCAGGTCTCCACCGACTA
TGTGTTCGACGGCGACAAGCCGGGACCCTATACAGAGGATGACCCGGTCGGTCCGATCGGGATCTATGGCGCCTCGAAGC
TGGCCGGCGAGATGGCCGTGCGCCTGGGCAATCCGCGCTCGGTCGTGCTGCGGACCGCCTGGGTGCTCAGCGCCCATCGC
GCCAACTTTCTGAAGACCATGCTCCGCCTCGCCGCCGACCGCCCGGTCGTGCGCGTCGTCGAAGACCAGCTGGGCTGTCC
GACCTCGGCGCGCGACATCGCCGAGGCGCTGCAAACCATCACGCTGGGGATGATCGCCGACCCACAAGCGCCGAACGGCG
TCTATCATTTCGTCAATTCGGGCGAGGCCAGCTGGGCGGATCTGGCGCGGGAAATCTTTCGTCTCAGCCACGCCCACGGA
GGCGTTCATGCCGAGGTCGAGGGCATTCCGAGTAGTGAATATCCAACGCCGGCCCGTCGTCCGCGTAACTCACGCCTGTT
GACCAACCGGATCGCAGCGGACTACGCCATCCACCCCCGGCCCTGGACAGAGGCCGTGGCGGACATCATCGCGGACTTGC
GCGTACAGGGAGCCCCTGCATGA

Upstream 100 bases:

>100_bases
CGTGGGCAGGGGGTCTGAGCGCTGCGGGCGCGCCGCGCGAGAGGACGATTGTCACCGTTGGCCTTGCTGGTCTGGTCGCG
GACCGCGTAGGTAGGGGCGA

Downstream 100 bases:

>100_bases
AGGGCATCATTCTCGCCGGCGGCTCGGGCACCCGGCTGCATCCGATGACCCTGGTCACGTCCAAGCAGCTGATGCCCGTC
TACGACAAGCCGATGATCTA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTDKTHILVTGGAGQVGLELLGAAWPDGIVLHAPSRAALDLTNETSIRAAFEARPFAAVINSAAWTAVDKAESEVAAAFA
ANAIGPALLAEVTRARGVPLIQVSTDYVFDGDKPGPYTEDDPVGPIGIYGASKLAGEMAVRLGNPRSVVLRTAWVLSAHR
ANFLKTMLRLAADRPVVRVVEDQLGCPTSARDIAEALQTITLGMIADPQAPNGVYHFVNSGEASWADLAREIFRLSHAHG
GVHAEVEGIPSSEYPTPARRPRNSRLLTNRIAADYAIHPRPWTEAVADIIADLRVQGAPA

Sequences:

>Translated_300_residues
MTDKTHILVTGGAGQVGLELLGAAWPDGIVLHAPSRAALDLTNETSIRAAFEARPFAAVINSAAWTAVDKAESEVAAAFA
ANAIGPALLAEVTRARGVPLIQVSTDYVFDGDKPGPYTEDDPVGPIGIYGASKLAGEMAVRLGNPRSVVLRTAWVLSAHR
ANFLKTMLRLAADRPVVRVVEDQLGCPTSARDIAEALQTITLGMIADPQAPNGVYHFVNSGEASWADLAREIFRLSHAHG
GVHAEVEGIPSSEYPTPARRPRNSRLLTNRIAADYAIHPRPWTEAVADIIADLRVQGAPA
>Mature_299_residues
TDKTHILVTGGAGQVGLELLGAAWPDGIVLHAPSRAALDLTNETSIRAAFEARPFAAVINSAAWTAVDKAESEVAAAFAA
NAIGPALLAEVTRARGVPLIQVSTDYVFDGDKPGPYTEDDPVGPIGIYGASKLAGEMAVRLGNPRSVVLRTAWVLSAHRA
NFLKTMLRLAADRPVVRVVEDQLGCPTSARDIAEALQTITLGMIADPQAPNGVYHFVNSGEASWADLAREIFRLSHAHGG
VHAEVEGIPSSEYPTPARRPRNSRLLTNRIAADYAIHPRPWTEAVADIIADLRVQGAPA

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=281, Percent_Identity=25.9786476868327, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI11034825, Length=281, Percent_Identity=25.9786476868327, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788352, Length=294, Percent_Identity=35.7142857142857, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31811; Mature: 31680

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTHILVTGGAGQVGLELLGAAWPDGIVLHAPSRAALDLTNETSIRAAFEARPFAAVI
CCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCEEEHHHCCCHHHHH
NSAAWTAVDKAESEVAAAFAANAIGPALLAEVTRARGVPLIQVSTDYVFDGDKPGPYTED
HCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCEEECCCCCCCCCCC
DPVGPIGIYGASKLAGEMAVRLGNPRSVVLRTAWVLSAHRANFLKTMLRLAADRPVVRVV
CCCCCEEECCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EDQLGCPTSARDIAEALQTITLGMIADPQAPNGVYHFVNSGEASWADLAREIFRLSHAHG
HHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
GVHAEVEGIPSSEYPTPARRPRNSRLLTNRIAADYAIHPRPWTEAVADIIADLRVQGAPA
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDKTHILVTGGAGQVGLELLGAAWPDGIVLHAPSRAALDLTNETSIRAAFEARPFAAVI
CCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCEEEHHHCCCHHHHH
NSAAWTAVDKAESEVAAAFAANAIGPALLAEVTRARGVPLIQVSTDYVFDGDKPGPYTED
HCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCEEECCCCCCCCCCC
DPVGPIGIYGASKLAGEMAVRLGNPRSVVLRTAWVLSAHRANFLKTMLRLAADRPVVRVV
CCCCCEEECCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EDQLGCPTSARDIAEALQTITLGMIADPQAPNGVYHFVNSGEASWADLAREIFRLSHAHG
HHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
GVHAEVEGIPSSEYPTPARRPRNSRLLTNRIAADYAIHPRPWTEAVADIIADLRVQGAPA
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]