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The map label for this gene is rmlA [H]

Identifier: 16125393

GI number: 16125393

Start: 1296591

End: 1297460

Strand: Direct

Name: rmlA [H]

Synonym: CC_1141

Alternate gene names: 16125393

Gene position: 1296591-1297460 (Clockwise)

Preceding gene: 16125392

Following gene: 16125399

Centisome position: 32.28

GC content: 64.48

Gene sequence:

>870_bases
ATGAAGGGCATCATTCTCGCCGGCGGCTCGGGCACCCGGCTGCATCCGATGACCCTGGTCACGTCCAAGCAGCTGATGCC
CGTCTACGACAAGCCGATGATCTATTATCCGCTGTCGACGCTGATGCTGGCGGGGATTCGCGAGATCCTGATCATCTCGA
CGCCGCGCGATACGCCCAACTTCCAGGCGCTGCTGGGCGATGGCTCCCAATGGGGCCTCGATATCCAGTACGCCGTGCAG
CCCAGCCCGGATGGCCTGGCCCAGGCCTATGTGATTGGCGCGGATTTCGTCGGCCGTGATCCCTCGGCGCTGATCCTGGG
CGACAATATCTATTTCGGTCACGGCATCACCAATCTGTTCACGCGGGCGATGGCGCGGCCCAAGGGGGCGACGGTGTTCG
CCTATCACGTCAACGATCCCGAACGGTACGGCGTGGTCGAGTTCGACAAGCAGATGACCGCACTCTCGATCGAGGAGAAG
CCGAAGGTTCCCAAGTCGAACTGGGCGGTGACGGGTCTGTACTTCTACGACGGCGATGTGGTCGACATCGCGCGGGACCT
CAAGCCGTCGGCGCGCGGCGAGCTTGAGATCACCGACGTCAATCGCGCCTATCTGGAGCGCGGCGACCTGTCGGTGGAGC
TGATGGGCCGGGGCTACGCCTGGCTCGACACCGGCACGCCCGACAGCCTGCTGGAGGCCGCCGAGTTCGTTCGCGTGCTG
GAAAAGCGCCAGGGCTTCAAGATCGCCTGCCCCGAAGAGGTGGCCTACCAGAAGGGCTTCATCGACGCCGCCCAGGTGGA
GCGCCTGGCCGACGCCTTGGGCAAGAGCGCCTATGGCAGCTATCTGCGTACCGTGATCGGCGTCCGCTAG

Upstream 100 bases:

>100_bases
TGACCAACCGGATCGCAGCGGACTACGCCATCCACCCCCGGCCCTGGACAGAGGCCGTGGCGGACATCATCGCGGACTTG
CGCGTACAGGGAGCCCCTGC

Downstream 100 bases:

>100_bases
ACTCGGAGGCCGGCGCTCGGGTTCGAGCGCCGGCCTCAACGCCTACATCGGCCCAACGCCGCCCGGCGTGGTCTGGAAGT
CGAAGGCCGGCTCGCGGGCC

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKGIILAGGSGTRLHPMTLVTSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPRDTPNFQALLGDGSQWGLDIQYAVQ
PSPDGLAQAYVIGADFVGRDPSALILGDNIYFGHGITNLFTRAMARPKGATVFAYHVNDPERYGVVEFDKQMTALSIEEK
PKVPKSNWAVTGLYFYDGDVVDIARDLKPSARGELEITDVNRAYLERGDLSVELMGRGYAWLDTGTPDSLLEAAEFVRVL
EKRQGFKIACPEEVAYQKGFIDAAQVERLADALGKSAYGSYLRTVIGVR

Sequences:

>Translated_289_residues
MKGIILAGGSGTRLHPMTLVTSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPRDTPNFQALLGDGSQWGLDIQYAVQ
PSPDGLAQAYVIGADFVGRDPSALILGDNIYFGHGITNLFTRAMARPKGATVFAYHVNDPERYGVVEFDKQMTALSIEEK
PKVPKSNWAVTGLYFYDGDVVDIARDLKPSARGELEITDVNRAYLERGDLSVELMGRGYAWLDTGTPDSLLEAAEFVRVL
EKRQGFKIACPEEVAYQKGFIDAAQVERLADALGKSAYGSYLRTVIGVR
>Mature_289_residues
MKGIILAGGSGTRLHPMTLVTSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPRDTPNFQALLGDGSQWGLDIQYAVQ
PSPDGLAQAYVIGADFVGRDPSALILGDNIYFGHGITNLFTRAMARPKGATVFAYHVNDPERYGVVEFDKQMTALSIEEK
PKVPKSNWAVTGLYFYDGDVVDIARDLKPSARGELEITDVNRAYLERGDLSVELMGRGYAWLDTGTPDSLLEAAEFVRVL
EKRQGFKIACPEEVAYQKGFIDAAQVERLADALGKSAYGSYLRTVIGVR

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761619, Length=277, Percent_Identity=25.2707581227437, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI11761621, Length=271, Percent_Identity=25.4612546125461, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788351, Length=281, Percent_Identity=71.1743772241993, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1790224, Length=289, Percent_Identity=64.7058823529412, Blast_Score=396, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI133931050, Length=239, Percent_Identity=24.2677824267782, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=253, Percent_Identity=29.2490118577075, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=24.7011952191235, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=24.7011952191235, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 31793; Mature: 31793

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLHPMTLVTSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPRDTPN
CCCEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCCC
FQALLGDGSQWGLDIQYAVQPSPDGLAQAYVIGADFVGRDPSALILGDNIYFGHGITNLF
CEEEECCCCCCCEEEEEEECCCCCCCEEEEEEECHHCCCCCCEEEEECCEEECCCHHHHH
TRAMARPKGATVFAYHVNDPERYGVVEFDKQMTALSIEEKPKVPKSNWAVTGLYFYDGDV
HHHHCCCCCCEEEEEECCCCCCCCEEEECCCEEEEECCCCCCCCCCCEEEEEEEEECCCH
VDIARDLKPSARGELEITDVNRAYLERGDLSVELMGRGYAWLDTGTPDSLLEAAEFVRVL
HHHHHHCCCCCCCCEEEEECCHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHH
EKRQGFKIACPEEVAYQKGFIDAAQVERLADALGKSAYGSYLRTVIGVR
HHCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGIILAGGSGTRLHPMTLVTSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPRDTPN
CCCEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCCC
FQALLGDGSQWGLDIQYAVQPSPDGLAQAYVIGADFVGRDPSALILGDNIYFGHGITNLF
CEEEECCCCCCCEEEEEEECCCCCCCEEEEEEECHHCCCCCCEEEEECCEEECCCHHHHH
TRAMARPKGATVFAYHVNDPERYGVVEFDKQMTALSIEEKPKVPKSNWAVTGLYFYDGDV
HHHHCCCCCCEEEEEECCCCCCCCEEEECCCEEEEECCCCCCCCCCCEEEEEEEEECCCH
VDIARDLKPSARGELEITDVNRAYLERGDLSVELMGRGYAWLDTGTPDSLLEAAEFVRVL
HHHHHHCCCCCCCCEEEEECCHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHH
EKRQGFKIACPEEVAYQKGFIDAAQVERLADALGKSAYGSYLRTVIGVR
HHCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]