Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 16124601

GI number: 16124601

Start: 366112

End: 368271

Strand: Direct

Name: priA [H]

Synonym: CC_0346

Alternate gene names: 16124601

Gene position: 366112-368271 (Clockwise)

Preceding gene: 16124597

Following gene: 16124603

Centisome position: 9.11

GC content: 71.25

Gene sequence:

>2160_bases
ATGCCGCGCATTGCTTCCGTCCTGCTGCCGATGCCGTTGCCGGAGGCCTTCGACTACGCCGAGCCGGAGGGCCTTGCGCT
GGCCGTCGGCGACCATGTGACCGTGCCGTTGGGACCGCGCGTCATCCGGGGCGTGGTGACGGCCCTGCGCGACGGGACCG
GCGGCAATCGGCCGCTGAAGCCGGTGCTGGAGCGGGTCGATGATCCGCCGCTGCCGCCGGGGGCTTTGGCGTTCGTCGAG
TGGGCGGCGCGCTACTCGGTCGATGTCCCTGGCTGGCCCTTGGCCATGGCCCTGCGCGGCCTGCGCCATCCGCCGCCCAA
GCCCGACAAGGTCCTGGTGCTGACGGGCGTTCAGCCCGCTCGCGTGACCCCGGCGCGGCTCAAGGTGATGGCCGCCGCAG
AGGGGACAAAGCTCTCGGGCGCGGCCCTGGCCTCGGCGGCGGGCGTCTCGGCGGGGGTGGTCAAGGGGCTGGTCGACGAG
GGCGTTCTGGCCGTCGATTTCGTGGAGCCCGAACGCGGCCTGCCGCAGCCCGACCTGTCGCTGCCCGCCCGGGCGCTCAA
CCCCGGCCAGGCCGCCTGTGTCGACGTGCTCAAGGACATGCTGGAGAGCGGCGGCTTCCAGGCCGCGCTGCTCGACGGTG
TGACCGGTTCGGGCAAGACCGAGGTCTATCTGGAAGCCGTCGCCGAGGCGCTGAAGGACCCCGAGACCCAGGTGCTGGTC
CTGTTGCCCGAGATCGCTCTGACCCAGGCGGTGATGGCCAGGTTCGAGCAACGATTCGGCGCGCTTCCCGCCGAGTGGCA
CTCGGGCGTCTCGCCGCCGCGTCGCCGCCAGGTCTGGGAGGCGGTCGCGGGCGGGAACGCGCGCATTGTCGTGGGCGCGC
GCTCGGCCCTGTTCCTGCCCTTCCGCAAACTGCGCCTGATCGTCGTGGACGAAGAGCACGACAGCTCGTTCAAGCAGGAA
GAAGGCTTCATCTATCAGGCCCGTGACCTCGCCGTGGCGCGCGCCAAGATCGAGGGCGCCAGTGTGCTTCTGGCCTCGGC
GACGCCGTCGCTGGAGAGCCTCTACAACGCCCAGACCGGGCGCTATCGCTGGCTGAGGCTGTCCGCTCGCCACGGCGCGG
CGCAACTGCCGGACATCGGTCTGGTCGATATGCGCCAGACCCCGCCGGAGCCCGGTCGCTGGCTGTCGCCGCCGCTGATC
AAGGCCATGGCCGTGACGCTGCAGCGCGGCGAGCAGGCCATGCTGTTCCTGAACCGACGCGGCTACGCCCCGCTCGTCCT
GTGCAAGGCCTGCGGCGAGAAGATGAAGTCGCCCGACACCGACAGCTGGCTGGTCGAGCACCGCTACACCGGGCGTCTGG
TCTGCCACCTGACGGGCTTTTCGATGAAGAAGCCCGAGGCCTGCCCGCACTGCGGGGCCAAGGACTCGCTGGTCTCGATC
GGCCCTGGCGTCGAGCGCGTCGAGGAAGAGGCGCGGCACATCTTCCCCGACGCCCGCGTGGCGGTGTTCTCTTCCGATAC
GGTGATGGACGCCGAGGGCGCCAAGGCGCTGGTGTCCAGCATGGCGGCCGGCGAGATCGACATCCTGGTGGCGACCCAGG
CCGCCGCCAAGGGCCACAACTTCCCGAACCTGACTCTGGTGGGCGTGGTCGACGCTGATCTGTCGCTACGGGGCGGTGAT
CTGCGGGCGGGCGAGCGGACGTTCCAGCTTCTGGCCCAGGCGGCGGGACGCGCGGGGCGGCACGAGAAGCCGGGGCGGGC
GCTGTTGCAGACCTACGCCCCCGACCATGCGGTGATGCGGGCCCTGGCGGCGCAGGATCGCGACGCCTTCGTCGAGGCCG
AGATGGCCATGCGCGAGGATGCTGGCCTGCCGCCGTTTGGACGTCTGGCGGCGGTGATCGCCTCGGGGCCGGACGGCGCG
GCGCTGGACGCCTATGTCGAGGCGCTGGCGGCGGTCATCCCCAACGCCGAGGGGGTCGAGGTGTTCGGCCCCGCCGATGC
GCCCCTGGCGCTGGTGCGCGGCCGACGGCGCAAGCGGTTCCTGGTGCGCGCCGAGCGCAATGTCGACCTGCAGGGCTTCA
TGGCCGCCTGGCGCGCCCGGGCCAAGGTTCCGAACTCTGTGCGGGTCGTGATCGACGTGGATCCCTACAGTTTCCTCTGA

Upstream 100 bases:

>100_bases
AGGCGACCAGATGAAGCGCCAAGACGGTTAACACCGGCTTGATGACGCCAAGACGCGGCGCTCTCGACCTGGGGACGGTT
TCGCGGCAGAAGCTCACGCC

Downstream 100 bases:

>100_bases
GGCGGTCGCGGGCGTTCAGACAACAAAAAAATCCCCGGAGCGAGCCGCCCCGGGGATTTCTGATCAAATCGCCTAAGCTG
AGAAACCTAGGTCTGCTGTT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MPRIASVLLPMPLPEAFDYAEPEGLALAVGDHVTVPLGPRVIRGVVTALRDGTGGNRPLKPVLERVDDPPLPPGALAFVE
WAARYSVDVPGWPLAMALRGLRHPPPKPDKVLVLTGVQPARVTPARLKVMAAAEGTKLSGAALASAAGVSAGVVKGLVDE
GVLAVDFVEPERGLPQPDLSLPARALNPGQAACVDVLKDMLESGGFQAALLDGVTGSGKTEVYLEAVAEALKDPETQVLV
LLPEIALTQAVMARFEQRFGALPAEWHSGVSPPRRRQVWEAVAGGNARIVVGARSALFLPFRKLRLIVVDEEHDSSFKQE
EGFIYQARDLAVARAKIEGASVLLASATPSLESLYNAQTGRYRWLRLSARHGAAQLPDIGLVDMRQTPPEPGRWLSPPLI
KAMAVTLQRGEQAMLFLNRRGYAPLVLCKACGEKMKSPDTDSWLVEHRYTGRLVCHLTGFSMKKPEACPHCGAKDSLVSI
GPGVERVEEEARHIFPDARVAVFSSDTVMDAEGAKALVSSMAAGEIDILVATQAAAKGHNFPNLTLVGVVDADLSLRGGD
LRAGERTFQLLAQAAGRAGRHEKPGRALLQTYAPDHAVMRALAAQDRDAFVEAEMAMREDAGLPPFGRLAAVIASGPDGA
ALDAYVEALAAVIPNAEGVEVFGPADAPLALVRGRRRKRFLVRAERNVDLQGFMAAWRARAKVPNSVRVVIDVDPYSFL

Sequences:

>Translated_719_residues
MPRIASVLLPMPLPEAFDYAEPEGLALAVGDHVTVPLGPRVIRGVVTALRDGTGGNRPLKPVLERVDDPPLPPGALAFVE
WAARYSVDVPGWPLAMALRGLRHPPPKPDKVLVLTGVQPARVTPARLKVMAAAEGTKLSGAALASAAGVSAGVVKGLVDE
GVLAVDFVEPERGLPQPDLSLPARALNPGQAACVDVLKDMLESGGFQAALLDGVTGSGKTEVYLEAVAEALKDPETQVLV
LLPEIALTQAVMARFEQRFGALPAEWHSGVSPPRRRQVWEAVAGGNARIVVGARSALFLPFRKLRLIVVDEEHDSSFKQE
EGFIYQARDLAVARAKIEGASVLLASATPSLESLYNAQTGRYRWLRLSARHGAAQLPDIGLVDMRQTPPEPGRWLSPPLI
KAMAVTLQRGEQAMLFLNRRGYAPLVLCKACGEKMKSPDTDSWLVEHRYTGRLVCHLTGFSMKKPEACPHCGAKDSLVSI
GPGVERVEEEARHIFPDARVAVFSSDTVMDAEGAKALVSSMAAGEIDILVATQAAAKGHNFPNLTLVGVVDADLSLRGGD
LRAGERTFQLLAQAAGRAGRHEKPGRALLQTYAPDHAVMRALAAQDRDAFVEAEMAMREDAGLPPFGRLAAVIASGPDGA
ALDAYVEALAAVIPNAEGVEVFGPADAPLALVRGRRRKRFLVRAERNVDLQGFMAAWRARAKVPNSVRVVIDVDPYSFL
>Mature_718_residues
PRIASVLLPMPLPEAFDYAEPEGLALAVGDHVTVPLGPRVIRGVVTALRDGTGGNRPLKPVLERVDDPPLPPGALAFVEW
AARYSVDVPGWPLAMALRGLRHPPPKPDKVLVLTGVQPARVTPARLKVMAAAEGTKLSGAALASAAGVSAGVVKGLVDEG
VLAVDFVEPERGLPQPDLSLPARALNPGQAACVDVLKDMLESGGFQAALLDGVTGSGKTEVYLEAVAEALKDPETQVLVL
LPEIALTQAVMARFEQRFGALPAEWHSGVSPPRRRQVWEAVAGGNARIVVGARSALFLPFRKLRLIVVDEEHDSSFKQEE
GFIYQARDLAVARAKIEGASVLLASATPSLESLYNAQTGRYRWLRLSARHGAAQLPDIGLVDMRQTPPEPGRWLSPPLIK
AMAVTLQRGEQAMLFLNRRGYAPLVLCKACGEKMKSPDTDSWLVEHRYTGRLVCHLTGFSMKKPEACPHCGAKDSLVSIG
PGVERVEEEARHIFPDARVAVFSSDTVMDAEGAKALVSSMAAGEIDILVATQAAAKGHNFPNLTLVGVVDADLSLRGGDL
RAGERTFQLLAQAAGRAGRHEKPGRALLQTYAPDHAVMRALAAQDRDAFVEAEMAMREDAGLPPFGRLAAVIASGPDGAA
LDAYVEALAAVIPNAEGVEVFGPADAPLALVRGRRRKRFLVRAERNVDLQGFMAAWRARAKVPNSVRVVIDVDPYSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=739, Percent_Identity=36.2652232746955, Blast_Score=373, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 76821; Mature: 76690

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIASVLLPMPLPEAFDYAEPEGLALAVGDHVTVPLGPRVIRGVVTALRDGTGGNRPLK
CCCHHHHHCCCCCCHHCCCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHCCCCCCCCHH
PVLERVDDPPLPPGALAFVEWAARYSVDVPGWPLAMALRGLRHPPPKPDKVLVLTGVQPA
HHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCC
RVTPARLKVMAAAEGTKLSGAALASAAGVSAGVVKGLVDEGVLAVDFVEPERGLPQPDLS
CCCHHHEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCC
LPARALNPGQAACVDVLKDMLESGGFQAALLDGVTGSGKTEVYLEAVAEALKDPETQVLV
CCCHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEE
LLPEIALTQAVMARFEQRFGALPAEWHSGVSPPRRRQVWEAVAGGNARIVVGARSALFLP
EECHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHCC
FRKLRLIVVDEEHDSSFKQEEGFIYQARDLAVARAKIEGASVLLASATPSLESLYNAQTG
CEEEEEEEEECCCCCCCHHHCCCEEEHHHHHHHHHEECCCEEEEEECCCCHHHHHCCCCC
RYRWLRLSARHGAAQLPDIGLVDMRQTPPEPGRWLSPPLIKAMAVTLQRGEQAMLFLNRR
CEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
GYAPLVLCKACGEKMKSPDTDSWLVEHRYTGRLVCHLTGFSMKKPEACPHCGAKDSLVSI
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEC
GPGVERVEEEARHIFPDARVAVFSSDTVMDAEGAKALVSSMAAGEIDILVATQAAAKGHN
CCCHHHHHHHHHHCCCCCEEEEEECCCEECCCHHHHHHHHHCCCCEEEEEEEHHHHCCCC
FPNLTLVGVVDADLSLRGGDLRAGERTFQLLAQAAGRAGRHEKPGRALLQTYAPDHAVMR
CCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHH
ALAAQDRDAFVEAEMAMREDAGLPPFGRLAAVIASGPDGAALDAYVEALAAVIPNAEGVE
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCEE
VFGPADAPLALVRGRRRKRFLVRAERNVDLQGFMAAWRARAKVPNSVRVVIDVDPYSFL
EECCCCCCHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
PRIASVLLPMPLPEAFDYAEPEGLALAVGDHVTVPLGPRVIRGVVTALRDGTGGNRPLK
CCHHHHHCCCCCCHHCCCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHCCCCCCCCHH
PVLERVDDPPLPPGALAFVEWAARYSVDVPGWPLAMALRGLRHPPPKPDKVLVLTGVQPA
HHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCC
RVTPARLKVMAAAEGTKLSGAALASAAGVSAGVVKGLVDEGVLAVDFVEPERGLPQPDLS
CCCHHHEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCC
LPARALNPGQAACVDVLKDMLESGGFQAALLDGVTGSGKTEVYLEAVAEALKDPETQVLV
CCCHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEE
LLPEIALTQAVMARFEQRFGALPAEWHSGVSPPRRRQVWEAVAGGNARIVVGARSALFLP
EECHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHCC
FRKLRLIVVDEEHDSSFKQEEGFIYQARDLAVARAKIEGASVLLASATPSLESLYNAQTG
CEEEEEEEEECCCCCCCHHHCCCEEEHHHHHHHHHEECCCEEEEEECCCCHHHHHCCCCC
RYRWLRLSARHGAAQLPDIGLVDMRQTPPEPGRWLSPPLIKAMAVTLQRGEQAMLFLNRR
CEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
GYAPLVLCKACGEKMKSPDTDSWLVEHRYTGRLVCHLTGFSMKKPEACPHCGAKDSLVSI
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEC
GPGVERVEEEARHIFPDARVAVFSSDTVMDAEGAKALVSSMAAGEIDILVATQAAAKGHN
CCCHHHHHHHHHHCCCCCEEEEEECCCEECCCHHHHHHHHHCCCCEEEEEEEHHHHCCCC
FPNLTLVGVVDADLSLRGGDLRAGERTFQLLAQAAGRAGRHEKPGRALLQTYAPDHAVMR
CCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHH
ALAAQDRDAFVEAEMAMREDAGLPPFGRLAAVIASGPDGAALDAYVEALAAVIPNAEGVE
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCEE
VFGPADAPLALVRGRRRKRFLVRAERNVDLQGFMAAWRARAKVPNSVRVVIDVDPYSFL
EECCCCCCHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]