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The map label for this gene is sucB [H]

Identifier: 16124595

GI number: 16124595

Start: 357720

End: 358928

Strand: Direct

Name: sucB [H]

Synonym: CC_0340

Alternate gene names: 16124595

Gene position: 357720-358928 (Clockwise)

Preceding gene: 161485653

Following gene: 16124597

Centisome position: 8.91

GC content: 69.56

Gene sequence:

>1209_bases
ATGGCCGACATCAATACGCCCGCCCTCGGCGAATCCGTCACCGAAGCCACGGTGGCGCGCTGGACCAAGAAGGTCGGTGA
GGCCGTGAAGAAGGACGAGATCCTCGTCGAGCTGGAAACCGACAAGGTTTCGCTCGAGGTGGCCTCGCCCGCCGACGGCG
TGCTGTCGGCGATCGGCGCTGCGGAAGGCGCGACCGTCGTTCCGGGCACGGTGCTGGGCGTCGTCGCCGAAGGCGCGACC
GCCTCGGCCGCGCCGGCCGCCGCGCCCGCGCCCAAGGCTGAAGCGCCCAAGCCGGCTCCGGCTGCCCCGGCCCCGGCCGC
GGCTCCCGCCGCCGCCCCGGTCAGCCCGGCCCCGGCCCGCATCGCCGCCGAGAGCGGCCTTGACCTCTCCAAGGTCGCCG
GCACCGGCAAGGACGGCCGCGTGACCAAGGGCGACGCCCTGGCGGCTCTGGAAGCCCGCGCCTCGGCCCCGGCCCCGGCC
GCCGCTGCGGCCGCGCCGCGCGCCCTGCACGAGCGCGAAGAGCGCGTGAAGATGACGCGCCTGCGTCAGACGATCGCCCG
TCGCCTGAAGGAAGCCCAGAACAGCGCCGCCATGCTGACGACCTTCAACGAGGTCGACATGAGCGCGGTGATGGCCCTGC
GCGCCCAGTACAAGGACGTGTTCGAAAAGCAGCACGGCGTGAAGCTGGGCTTCATGTCGTTCTTCGTGAAGGCCGTCGTC
GCGGCCCTGAAGGCGATCCCGGACGTCAACGCCGAGATCGACGGTCAGGACGTCATCTACAAGAACCACTACGACATCGG
CGTCGCCGTCGGCACCGACAAGGGCCTGGTGGTTCCGGTCGTCCGTGACGCCGACGCCCTGAACCTGGCCGGTATCGAAA
AGACCATCGGCGACCTCGGCAAGCGCGCCCGCAACGGCCAGCTGGCCATCGAGGACATGCAGGGCGGCACCTTCACGATC
ACCAACGGCGGCATCTACGGCTCGCTGATGTCGACCCCGATCCTGAACGCGCCGCAGTCGGGGATCCTGGGCATGCACGC
CATCAAGGAACGCCCGATGGTCATCAACGGCAAGATCGAGATCCGCCCGATGATGTACCTGGCTCTGTCCTACGATCACC
GCATCGTCGACGGCGCCGGCGCCGTGACCTTCCTGGTGAAGGTCAAGGAAGCCATCGAAGACCCGCAGCGCCTGCTGCTG
GAGCTCTAG

Upstream 100 bases:

>100_bases
TGAAAGAGCTCGAAACCTTCCTCAACGAGGCCTTCGCGTAAGCGAAGGGCTCGTACCCAAGAAACCCGCCGGACCAAGCA
AGAGAGACTCGGAAAGCCCC

Downstream 100 bases:

>100_bases
GAGACCTTTCGGAAGCCCGCCTCACCCGGCGGGCTTCTTGATTTTGGCGGAGGCTCCCCCTGTCCAAGGATATGCTCTAC
CTGCTTCGGGCCGGGTTTCC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MADINTPALGESVTEATVARWTKKVGEAVKKDEILVELETDKVSLEVASPADGVLSAIGAAEGATVVPGTVLGVVAEGAT
ASAAPAAAPAPKAEAPKPAPAAPAPAAAPAAAPVSPAPARIAAESGLDLSKVAGTGKDGRVTKGDALAALEARASAPAPA
AAAAAPRALHEREERVKMTRLRQTIARRLKEAQNSAAMLTTFNEVDMSAVMALRAQYKDVFEKQHGVKLGFMSFFVKAVV
AALKAIPDVNAEIDGQDVIYKNHYDIGVAVGTDKGLVVPVVRDADALNLAGIEKTIGDLGKRARNGQLAIEDMQGGTFTI
TNGGIYGSLMSTPILNAPQSGILGMHAIKERPMVINGKIEIRPMMYLALSYDHRIVDGAGAVTFLVKVKEAIEDPQRLLL
EL

Sequences:

>Translated_402_residues
MADINTPALGESVTEATVARWTKKVGEAVKKDEILVELETDKVSLEVASPADGVLSAIGAAEGATVVPGTVLGVVAEGAT
ASAAPAAAPAPKAEAPKPAPAAPAPAAAPAAAPVSPAPARIAAESGLDLSKVAGTGKDGRVTKGDALAALEARASAPAPA
AAAAAPRALHEREERVKMTRLRQTIARRLKEAQNSAAMLTTFNEVDMSAVMALRAQYKDVFEKQHGVKLGFMSFFVKAVV
AALKAIPDVNAEIDGQDVIYKNHYDIGVAVGTDKGLVVPVVRDADALNLAGIEKTIGDLGKRARNGQLAIEDMQGGTFTI
TNGGIYGSLMSTPILNAPQSGILGMHAIKERPMVINGKIEIRPMMYLALSYDHRIVDGAGAVTFLVKVKEAIEDPQRLLL
EL
>Mature_401_residues
ADINTPALGESVTEATVARWTKKVGEAVKKDEILVELETDKVSLEVASPADGVLSAIGAAEGATVVPGTVLGVVAEGATA
SAAPAAAPAPKAEAPKPAPAAPAPAAAPAAAPVSPAPARIAAESGLDLSKVAGTGKDGRVTKGDALAALEARASAPAPAA
AAAAPRALHEREERVKMTRLRQTIARRLKEAQNSAAMLTTFNEVDMSAVMALRAQYKDVFEKQHGVKLGFMSFFVKAVVA
ALKAIPDVNAEIDGQDVIYKNHYDIGVAVGTDKGLVVPVVRDADALNLAGIEKTIGDLGKRARNGQLAIEDMQGGTFTIT
NGGIYGSLMSTPILNAPQSGILGMHAIKERPMVINGKIEIRPMMYLALSYDHRIVDGAGAVTFLVKVKEAIEDPQRLLLE
L

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=246, Percent_Identity=59.349593495935, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=30.4347826086957, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=28.8888888888889, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=28.8888888888889, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=27.9620853080569, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=36.5269461077844, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=55.8312655086849, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=31.6901408450704, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=420, Percent_Identity=44.7619047619048, Blast_Score=319, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=30.9468822170901, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=27.4418604651163, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=317, Percent_Identity=28.7066246056782, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=397, Percent_Identity=45.088161209068, Blast_Score=340, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=30.9576837416481, Blast_Score=170, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=58.2959641255605, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=29.4663573085847, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=31.1688311688312, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24582497, Length=290, Percent_Identity=30.6896551724138, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 41650; Mature: 41519

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADINTPALGESVTEATVARWTKKVGEAVKKDEILVELETDKVSLEVASPADGVLSAIGA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCHHHHHHHHC
AEGATVVPGTVLGVVAEGATASAAPAAAPAPKAEAPKPAPAAPAPAAAPAAAPVSPAPAR
CCCCEECCCHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
IAAESGLDLSKVAGTGKDGRVTKGDALAALEARASAPAPAAAAAAPRALHEREERVKMTR
HHHHCCCCHHHHCCCCCCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LRQTIARRLKEAQNSAAMLTTFNEVDMSAVMALRAQYKDVFEKQHGVKLGFMSFFVKAVV
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
AALKAIPDVNAEIDGQDVIYKNHYDIGVAVGTDKGLVVPVVRDADALNLAGIEKTIGDLG
HHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHH
KRARNGQLAIEDMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHAIKERPMVINGKIE
HHCCCCCEEEEECCCCEEEEECCCEEEHHHHCCCCCCCCCCCHHHHHHCCCCEEEECEEE
IRPMMYLALSYDHRIVDGAGAVTFLVKVKEAIEDPQRLLLEL
EEEEEEEEEECCCEEECCCCHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
ADINTPALGESVTEATVARWTKKVGEAVKKDEILVELETDKVSLEVASPADGVLSAIGA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCHHHHHHHHC
AEGATVVPGTVLGVVAEGATASAAPAAAPAPKAEAPKPAPAAPAPAAAPAAAPVSPAPAR
CCCCEECCCHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
IAAESGLDLSKVAGTGKDGRVTKGDALAALEARASAPAPAAAAAAPRALHEREERVKMTR
HHHHCCCCHHHHCCCCCCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LRQTIARRLKEAQNSAAMLTTFNEVDMSAVMALRAQYKDVFEKQHGVKLGFMSFFVKAVV
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
AALKAIPDVNAEIDGQDVIYKNHYDIGVAVGTDKGLVVPVVRDADALNLAGIEKTIGDLG
HHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHH
KRARNGQLAIEDMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHAIKERPMVINGKIE
HHCCCCCEEEEECCCCEEEEECCCEEEHHHHCCCCCCCCCCCHHHHHHCCCCEEEECEEE
IRPMMYLALSYDHRIVDGAGAVTFLVKVKEAIEDPQRLLLEL
EEEEEEEEEECCCEEECCCCHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]