Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 16124549

GI number: 16124549

Start: 310374

End: 311066

Strand: Direct

Name: phoB [H]

Synonym: CC_0294

Alternate gene names: 16124549

Gene position: 310374-311066 (Clockwise)

Preceding gene: 16124548

Following gene: 16124552

Centisome position: 7.73

GC content: 67.39

Gene sequence:

>693_bases
GTGACTCCCTACGTTTTGGTGGTCGAAGACGAAGACGCTCTGGCCACTCTGCTCCACTACAACCTCGACAAGGAGGGCTA
CCGCGTCGGCGTCGCCGGCGACGGTGAGGAAGCCCTGATCATGGCCAATGAGCGGGCCCCGGACCTGGTCATCCTCGACT
GGATGCTGCCCAAGGTCTCGGGCATCGAGGTCTGCCGCCGCCTGCGCGGCCGCGCCGAGACGCGCAATGTGCCGATCATC
ATGCTGACGGCCCGCGGCGAGGAAACTGATCGCATCCGCGGTCTCGACACCGGCGCCGACGACTATGTGGTCAAGCCGTT
CTCGATGGTCGAGCTGACCGCCCGGGTCCGCGCCGTGCTGCGCCGCATCCGCCCGGGTCTGGCCGATGACCGCATCACCG
TCGGCGACATCATCATCGACCGCGTCGCCCACAGGGTGAAGCGCAGCGGCAAGGAGATCCACCTTGGCCCGACCGAGTTC
CGTCTGCTCGACTACCTGATGCAGCACCCGGGCCGGGTGTTCAGCCGCGAGCAGCTGCTGGACGCGGTCTGGGGGTCGGA
CGTCTATGTCGAGGCCCGCACGGTCGACGTTCACATCGGCCGTCTTCGCAAGGCGCTGAACGGGACGACGGACGCCGATC
CCATCCGCACCGTGCGTTCGGCGGGCTACTCGCTGGACATGGATGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCTGAAATCATCCATTTCGAGCTGACGGGTGAGGAACTGACCTCGCAACGTCCCAAGCTCGACGTGCTGTCGCAATAAC
TCTGGAAGAGGCGGCCTGAA

Downstream 100 bases:

>100_bases
GAGTCTGAGGCCTGTCGACCGAGGCGTTTGAGCAACGCGTCGCGGTAGGTTCGGCTGGTCACGACCTCCGCGCCGCTGGC
CAGGGTCAGCCGCCAGGAGC

Product: phosphate regulon response regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTPYVLVVEDEDALATLLHYNLDKEGYRVGVAGDGEEALIMANERAPDLVILDWMLPKVSGIEVCRRLRGRAETRNVPII
MLTARGEETDRIRGLDTGADDYVVKPFSMVELTARVRAVLRRIRPGLADDRITVGDIIIDRVAHRVKRSGKEIHLGPTEF
RLLDYLMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHIGRLRKALNGTTDADPIRTVRSAGYSLDMDAA

Sequences:

>Translated_230_residues
MTPYVLVVEDEDALATLLHYNLDKEGYRVGVAGDGEEALIMANERAPDLVILDWMLPKVSGIEVCRRLRGRAETRNVPII
MLTARGEETDRIRGLDTGADDYVVKPFSMVELTARVRAVLRRIRPGLADDRITVGDIIIDRVAHRVKRSGKEIHLGPTEF
RLLDYLMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHIGRLRKALNGTTDADPIRTVRSAGYSLDMDAA
>Mature_229_residues
TPYVLVVEDEDALATLLHYNLDKEGYRVGVAGDGEEALIMANERAPDLVILDWMLPKVSGIEVCRRLRGRAETRNVPIIM
LTARGEETDRIRGLDTGADDYVVKPFSMVELTARVRAVLRRIRPGLADDRITVGDIIIDRVAHRVKRSGKEIHLGPTEFR
LLDYLMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHIGRLRKALNGTTDADPIRTVRSAGYSLDMDAA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=221, Percent_Identity=49.7737556561086, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=44, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=40.5172413793103, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=37.6681614349776, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786911, Length=213, Percent_Identity=41.7840375586854, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789809, Length=237, Percent_Identity=35.8649789029536, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=36.6515837104072, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI2367329, Length=227, Percent_Identity=40.0881057268723, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=34.5291479820628, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=35.4978354978355, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=33.4841628959276, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=37.0535714285714, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=34.5991561181435, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=32.3275862068966, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=34.1880341880342, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25775; Mature: 25644

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPYVLVVEDEDALATLLHYNLDKEGYRVGVAGDGEEALIMANERAPDLVILDWMLPKVS
CCCEEEEEECCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCCEEEEECCCCCCC
GIEVCRRLRGRAETRNVPIIMLTARGEETDRIRGLDTGADDYVVKPFSMVELTARVRAVL
HHHHHHHHHCCCHHCCCCEEEEEECCCCCHHEECCCCCCCCEEECCHHHHHHHHHHHHHH
RRIRPGLADDRITVGDIIIDRVAHRVKRSGKEIHLGPTEFRLLDYLMQHPGRVFSREQLL
HHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHH
DAVWGSDVYVEARTVDVHIGRLRKALNGTTDADPIRTVRSAGYSLDMDAA
HHHCCCCEEEEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
TPYVLVVEDEDALATLLHYNLDKEGYRVGVAGDGEEALIMANERAPDLVILDWMLPKVS
CCEEEEEECCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCCEEEEECCCCCCC
GIEVCRRLRGRAETRNVPIIMLTARGEETDRIRGLDTGADDYVVKPFSMVELTARVRAVL
HHHHHHHHHCCCHHCCCCEEEEEECCCCCHHEECCCCCCCCEEECCHHHHHHHHHHHHHH
RRIRPGLADDRITVGDIIIDRVAHRVKRSGKEIHLGPTEFRLLDYLMQHPGRVFSREQLL
HHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHH
DAVWGSDVYVEARTVDVHIGRLRKALNGTTDADPIRTVRSAGYSLDMDAA
HHHCCCCEEEEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]