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The map label for this gene is purD

Identifier: 16124552

GI number: 16124552

Start: 312678

End: 313961

Strand: Direct

Name: purD

Synonym: CC_0297

Alternate gene names: 16124552

Gene position: 312678-313961 (Clockwise)

Preceding gene: 16124549

Following gene: 16124554

Centisome position: 7.78

GC content: 70.4

Gene sequence:

>1284_bases
ATGGAAAAGCTGACCATCCTCCTCGTCGGGTCCGGCGGGCGCGAGCACGCCCTGGCCTGGAAGATCGCCCAGTCGCCGCT
GTGCGGCCGCCTTGTCGCGGCGCCCGGCAATCCCGGTATCGAAGCCGTGGCCGAGCTGCGTGCGGTCAAGGCGACCGACG
CCGATGGCCTCGTCGCCCTGGCTCAGGAGATCGGCGCGGATCTGGTGGTGGTCGGTCCGGAGTCGGCGCTGGAGGTCGGT
CTCGCCGATAAGCTGGCGGCGGTCGGTATCCCCTGTTTTGGCGGCAGCCAGCGCGCCGCGCAGCTGGAGACGTCCAAGGC
CTTCACCAAGGACTTCTGTCAGCGTCACGGCCTGCCGACGGCGGCCTATGGCGTGTTCGAGGACGCCGCCTCGGCCGGCG
CCTTCCTCGACACCCTGGAAGCGCCGTTCGTGATCAAGGCCGACGGTCTGGCGGCCGGCAAGGGCGTGGTCATCGCCCCC
GACCGCGCCGCAGCTGACGCCGCCGTGGTCGACATGTTGGGCGGCCGTTTCGGCTCGGCCGGCGCGCGGGTGGTGATCGA
GGAGTTCATGCACGGCGAGGAGGCCTCGCTGTTCGCGGTCTGCGACGGCAAGACCGCCGTGCTGTTCGGCGCGGCCCAGG
ACCACAAGCGCGCCTATGACGGCGACGAGGGCCCCAACACCGGCGGCATGGGCACCTATTCGCCGCCGCCCGTCCTGACC
GACGCCCTGATCGACCAGGCCTGGCGCGAGCTGATCGTGCCGACCGTCGAGGGCATGGCCGCCGAGGGCAATCCCTATGT
CGGCGTGCTCTATGCCGGGCTGATGCTGACCCCGACGGGGCCGAAGCTCGTGGAGTATAACGCCCGCTTCGGCGATCCCG
AGTGCCAGACCCTGATGCTGCGCCTGGATAGCGACATCGTCCCGATCCTGCTGGCCGCCGCCAAGGGCGAGCTGGCCAAC
GCCGAACCGCCGAAGTGGCGCGAGGAGGCGGCGATCTGCGTGGTGCTGGCCGCCGAGGGCTATCCTGACGCGCCGAAGAC
CGGCGGTCGCATCCAGGGCGCCGACGCCGATTTTGGCGACGACGTCGTGGTCTTCCACGCCGGTACGACGCGCGAGTTCG
AGGGACGTCTGGTGGCCTCGGGCGGTCGGGTGCTGAATGTCTGCGCCCTGGGCGCGACCCTGAGTGAGGCTCGCGAGGCC
GCCTATGGCGCGCTGGAGACGATCAGCCTGGAAGGCGGTTTCTATCGCACCGACATCGGTTGGCGGGCGCTGCAGCAGCG
CTGA

Upstream 100 bases:

>100_bases
GGACGGCCGACGTCGTCGCCAGCGCGATGGGGCCAATGGCGGCCTGCGGGGGCGAGAGCGCGCTTGACCCGATGCCGGTC
GCACGTCACACCCCGCGCTC

Downstream 100 bases:

>100_bases
GCGCCCGCCGCCGCCGTCAGGTCCGGTGCGCCACCTCCACCAGAATCTCGCCCGGCGCGGTCACATAGAAGCTGAACGCT
CCGCGCTGCACGCAGGCCTC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MEKLTILLVGSGGREHALAWKIAQSPLCGRLVAAPGNPGIEAVAELRAVKATDADGLVALAQEIGADLVVVGPESALEVG
LADKLAAVGIPCFGGSQRAAQLETSKAFTKDFCQRHGLPTAAYGVFEDAASAGAFLDTLEAPFVIKADGLAAGKGVVIAP
DRAAADAAVVDMLGGRFGSAGARVVIEEFMHGEEASLFAVCDGKTAVLFGAAQDHKRAYDGDEGPNTGGMGTYSPPPVLT
DALIDQAWRELIVPTVEGMAAEGNPYVGVLYAGLMLTPTGPKLVEYNARFGDPECQTLMLRLDSDIVPILLAAAKGELAN
AEPPKWREEAAICVVLAAEGYPDAPKTGGRIQGADADFGDDVVVFHAGTTREFEGRLVASGGRVLNVCALGATLSEAREA
AYGALETISLEGGFYRTDIGWRALQQR

Sequences:

>Translated_427_residues
MEKLTILLVGSGGREHALAWKIAQSPLCGRLVAAPGNPGIEAVAELRAVKATDADGLVALAQEIGADLVVVGPESALEVG
LADKLAAVGIPCFGGSQRAAQLETSKAFTKDFCQRHGLPTAAYGVFEDAASAGAFLDTLEAPFVIKADGLAAGKGVVIAP
DRAAADAAVVDMLGGRFGSAGARVVIEEFMHGEEASLFAVCDGKTAVLFGAAQDHKRAYDGDEGPNTGGMGTYSPPPVLT
DALIDQAWRELIVPTVEGMAAEGNPYVGVLYAGLMLTPTGPKLVEYNARFGDPECQTLMLRLDSDIVPILLAAAKGELAN
AEPPKWREEAAICVVLAAEGYPDAPKTGGRIQGADADFGDDVVVFHAGTTREFEGRLVASGGRVLNVCALGATLSEAREA
AYGALETISLEGGFYRTDIGWRALQQR
>Mature_427_residues
MEKLTILLVGSGGREHALAWKIAQSPLCGRLVAAPGNPGIEAVAELRAVKATDADGLVALAQEIGADLVVVGPESALEVG
LADKLAAVGIPCFGGSQRAAQLETSKAFTKDFCQRHGLPTAAYGVFEDAASAGAFLDTLEAPFVIKADGLAAGKGVVIAP
DRAAADAAVVDMLGGRFGSAGARVVIEEFMHGEEASLFAVCDGKTAVLFGAAQDHKRAYDGDEGPNTGGMGTYSPPPVLT
DALIDQAWRELIVPTVEGMAAEGNPYVGVLYAGLMLTPTGPKLVEYNARFGDPECQTLMLRLDSDIVPILLAAAKGELAN
AEPPKWREEAAICVVLAAEGYPDAPKTGGRIQGADADFGDDVVVFHAGTTREFEGRLVASGGRVLNVCALGATLSEAREA
AYGALETISLEGGFYRTDIGWRALQQR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=43.0260047281324, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=43.0260047281324, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=43.0260047281324, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=43.0260047281324, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=49.7663551401869, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=39.8104265402844, Blast_Score=271, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6321203, Length=441, Percent_Identity=40.1360544217687, Blast_Score=308, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=42.5581395348837, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=42.5581395348837, Blast_Score=301, Evalue=7e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_CAUCR (Q9ABD2)

Other databases:

- EMBL:   AE005673
- PIR:   H87285
- RefSeq:   NP_419116.1
- ProteinModelPortal:   Q9ABD2
- SMR:   Q9ABD2
- GeneID:   942342
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_0297
- NMPDR:   fig|190650.1.peg.296
- TIGR:   CC_0297
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 44266; Mature: 44266

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLTILLVGSGGREHALAWKIAQSPLCGRLVAAPGNPGIEAVAELRAVKATDADGLVAL
CCEEEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHH
AQEIGADLVVVGPESALEVGLADKLAAVGIPCFGGSQRAAQLETSKAFTKDFCQRHGLPT
HHHHCCCEEEECCCHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
AAYGVFEDAASAGAFLDTLEAPFVIKADGLAAGKGVVIAPDRAAADAAVVDMLGGRFGSA
HHHHHHHHHHHCCHHHHHCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCC
GARVVIEEFMHGEEASLFAVCDGKTAVLFGAAQDHKRAYDGDEGPNTGGMGTYSPPPVLT
CHHHHHHHHHCCCCCEEEEEECCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCHHH
DALIDQAWRELIVPTVEGMAAEGNPYVGVLYAGLMLTPTGPKLVEYNARFGDPECQTLML
HHHHHHHHHHHHCCCCCCEECCCCCEEEEEEECEEECCCCCCEEEECCCCCCCHHHEEEE
RLDSDIVPILLAAAKGELANAEPPKWREEAAICVVLAAEGYPDAPKTGGRIQGADADFGD
ECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCC
DVVVFHAGTTREFEGRLVASGGRVLNVCALGATLSEAREAAYGALETISLEGGFYRTDIG
CEEEEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHCCEEEEEECCCEEEECCC
WRALQQR
HHHHHCC
>Mature Secondary Structure
MEKLTILLVGSGGREHALAWKIAQSPLCGRLVAAPGNPGIEAVAELRAVKATDADGLVAL
CCEEEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHH
AQEIGADLVVVGPESALEVGLADKLAAVGIPCFGGSQRAAQLETSKAFTKDFCQRHGLPT
HHHHCCCEEEECCCHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
AAYGVFEDAASAGAFLDTLEAPFVIKADGLAAGKGVVIAPDRAAADAAVVDMLGGRFGSA
HHHHHHHHHHHCCHHHHHCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCC
GARVVIEEFMHGEEASLFAVCDGKTAVLFGAAQDHKRAYDGDEGPNTGGMGTYSPPPVLT
CHHHHHHHHHCCCCCEEEEEECCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCHHH
DALIDQAWRELIVPTVEGMAAEGNPYVGVLYAGLMLTPTGPKLVEYNARFGDPECQTLML
HHHHHHHHHHHHCCCCCCEECCCCCEEEEEEECEEECCCCCCEEEECCCCCCCHHHEEEE
RLDSDIVPILLAAAKGELANAEPPKWREEAAICVVLAAEGYPDAPKTGGRIQGADADFGD
ECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCC
DVVVFHAGTTREFEGRLVASGGRVLNVCALGATLSEAREAAYGALETISLEGGFYRTDIG
CEEEEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHCCEEEEEECCCEEEECCC
WRALQQR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647