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The map label for this gene is dapE

Identifier: 16124530

GI number: 16124530

Start: 290161

End: 291321

Strand: Direct

Name: dapE

Synonym: CC_0275

Alternate gene names: 16124530

Gene position: 290161-291321 (Clockwise)

Preceding gene: 16124527

Following gene: 16124531

Centisome position: 7.22

GC content: 69.42

Gene sequence:

>1161_bases
ATGACCAGCCCCGCGCCCGTTTCCGTCAGCATCGATCCCGTTGAGCTCGCTCAAGCCCTGATCCGGCGTCCGTCGGTGAC
GCCGGCCGACGCCGGCGCGATGGACACGCTGCAGCGCCAACTGGAGGCGCTCGGGTTCGCCTGCCGCCGGATGAAGTTCG
GCGAGATCGAGAACCTCTACGCCCGGCGCGGGACGGCGCGGCCGAACCTCTGTTTCGCCGGCCATACCGATGTGGTGCCG
GTGGGCGATGACGCGGCCTGGACCGCTGGGCCGTTCGAGGCCGAGATCAAGGAGGGCGTGCTCTATGGGCGCGGCGCGGT
CGACATGAAGTCGGCCATCGCCGCCTTCGTCGCCGCCGTGGCGAACGTCCCCGACCATCCCGGCTCGATCAGCTTCCTGA
TCACCGGCGACGAGGAAGGAGTGGCCGAGGACGGCACGGTCAAGGTCGTCGAGGCCCTGGCCGCCGAGGGCGAGATCATC
GACCACTGCATCGTCGGCGAGCCGACCTCGGCGAACCTCTTGGGCGACATGGTCAAGATCGGCCGGCGCGGCAGCATCAA
CGCCTGGATCACCGTCGAGGGCCGCCAGGGCCACGTGGCCTATCCGCATCGCGCGGCCAATCCGGTTCCGGTGCTGGTCG
ACATCCTGTCGGCCCTGAAGGCGCGGGTGCTGGACGACGGCTATACCGGCTTCCAGCCGTCGAACCTGGAGATCACCACG
ATCGACGTCGGCAACACCGCCACCAACGTCATCCCCGCCGCCGCCAAGGCGCGCGTGAACATCCGCTTCAACCCGGCCCA
CAAGGGCAAGGACCTGGCCGCCTGGATCGAGGGCGAGTGCGCCAAGGCTGCGGAAGGTTTCGACGGCGCAGCCACCGCGC
TGTGCAAGATCAGCGGCGAGGCGTTCTTGACCGAGCCGGGCGACTTCACCGACGTGATCGTGGCGGCCGTCACCGACGCC
ACCGGCCGCGCGCCGGAGCTGTCGACTACCGGCGGCACCAGCGACGCCCGCTTCATTCGCGCCCTGTGCCCCGTCGTCGA
GTTCGGCCTGGTCGGCTCGACCATGCACCAGGTCGATGAGCGGGTCCCGGTCGAGGAGGTTCGCCAGCTGGCGGGCGCCT
ATGAGGCGCTGATCCGCCGCTACTTCGCAGCATTCGCCTAA

Upstream 100 bases:

>100_bases
GCGGGTGGGGGCGGCGGCGAGATCGATCTTGGGCATGACCTACCTTGGAACAAGCGTGCGGGATTTGCGCGGACGCAAGG
GGAAGCTTAAAGCCTGCACC

Downstream 100 bases:

>100_bases
GCCGCGCGGGGACGAGGATGAAGGCGCTCTCGATCCCCGATGTGCTGGCCGAGGTGGCGGTGCTGGTCAGGCCGCACTTC
GGCAAAGGCAAGCCCGCCGA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTSPAPVSVSIDPVELAQALIRRPSVTPADAGAMDTLQRQLEALGFACRRMKFGEIENLYARRGTARPNLCFAGHTDVVP
VGDDAAWTAGPFEAEIKEGVLYGRGAVDMKSAIAAFVAAVANVPDHPGSISFLITGDEEGVAEDGTVKVVEALAAEGEII
DHCIVGEPTSANLLGDMVKIGRRGSINAWITVEGRQGHVAYPHRAANPVPVLVDILSALKARVLDDGYTGFQPSNLEITT
IDVGNTATNVIPAAAKARVNIRFNPAHKGKDLAAWIEGECAKAAEGFDGAATALCKISGEAFLTEPGDFTDVIVAAVTDA
TGRAPELSTTGGTSDARFIRALCPVVEFGLVGSTMHQVDERVPVEEVRQLAGAYEALIRRYFAAFA

Sequences:

>Translated_386_residues
MTSPAPVSVSIDPVELAQALIRRPSVTPADAGAMDTLQRQLEALGFACRRMKFGEIENLYARRGTARPNLCFAGHTDVVP
VGDDAAWTAGPFEAEIKEGVLYGRGAVDMKSAIAAFVAAVANVPDHPGSISFLITGDEEGVAEDGTVKVVEALAAEGEII
DHCIVGEPTSANLLGDMVKIGRRGSINAWITVEGRQGHVAYPHRAANPVPVLVDILSALKARVLDDGYTGFQPSNLEITT
IDVGNTATNVIPAAAKARVNIRFNPAHKGKDLAAWIEGECAKAAEGFDGAATALCKISGEAFLTEPGDFTDVIVAAVTDA
TGRAPELSTTGGTSDARFIRALCPVVEFGLVGSTMHQVDERVPVEEVRQLAGAYEALIRRYFAAFA
>Mature_385_residues
TSPAPVSVSIDPVELAQALIRRPSVTPADAGAMDTLQRQLEALGFACRRMKFGEIENLYARRGTARPNLCFAGHTDVVPV
GDDAAWTAGPFEAEIKEGVLYGRGAVDMKSAIAAFVAAVANVPDHPGSISFLITGDEEGVAEDGTVKVVEALAAEGEIID
HCIVGEPTSANLLGDMVKIGRRGSINAWITVEGRQGHVAYPHRAANPVPVLVDILSALKARVLDDGYTGFQPSNLEITTI
DVGNTATNVIPAAAKARVNIRFNPAHKGKDLAAWIEGECAKAAEGFDGAATALCKISGEAFLTEPGDFTDVIVAAVTDAT
GRAPELSTTGGTSDARFIRALCPVVEFGLVGSTMHQVDERVPVEEVRQLAGAYEALIRRYFAAFA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=43.7333333333333, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI1790395, Length=383, Percent_Identity=27.9373368146214, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_CAUCN (B8GYE7)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002515652.1
- ProteinModelPortal:   B8GYE7
- SMR:   B8GYE7
- MEROPS:   M20.010
- GeneID:   7330730
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_00277
- OMA:   ARNPVHQ
- ProtClustDB:   PRK13009
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40607; Mature: 40476

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 77-77 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPAPVSVSIDPVELAQALIRRPSVTPADAGAMDTLQRQLEALGFACRRMKFGEIENLY
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ARRGTARPNLCFAGHTDVVPVGDDAAWTAGPFEAEIKEGVLYGRGAVDMKSAIAAFVAAV
HHCCCCCCCEEECCCCCEEECCCCCCCCCCCCHHHHHCCEEECCCCHHHHHHHHHHHHHH
ANVPDHPGSISFLITGDEEGVAEDGTVKVVEALAAEGEIIDHCIVGEPTSANLLGDMVKI
HCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEHHEECCCCCCHHHHHHHHHH
GRRGSINAWITVEGRQGHVAYPHRAANPVPVLVDILSALKARVLDDGYTGFQPSNLEITT
CCCCCCEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
IDVGNTATNVIPAAAKARVNIRFNPAHKGKDLAAWIEGECAKAAEGFDGAATALCKISGE
EECCCCHHHHCCCCCEEEEEEEECCCCCCCCEEEHHCCCHHHHHCCCCHHHHEEEEECCC
AFLTEPGDFTDVIVAAVTDATGRAPELSTTGGTSDARFIRALCPVVEFGLVGSTMHQVDE
EEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVPVEEVRQLAGAYEALIRRYFAAFA
CCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSPAPVSVSIDPVELAQALIRRPSVTPADAGAMDTLQRQLEALGFACRRMKFGEIENLY
CCCCCEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ARRGTARPNLCFAGHTDVVPVGDDAAWTAGPFEAEIKEGVLYGRGAVDMKSAIAAFVAAV
HHCCCCCCCEEECCCCCEEECCCCCCCCCCCCHHHHHCCEEECCCCHHHHHHHHHHHHHH
ANVPDHPGSISFLITGDEEGVAEDGTVKVVEALAAEGEIIDHCIVGEPTSANLLGDMVKI
HCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEHHEECCCCCCHHHHHHHHHH
GRRGSINAWITVEGRQGHVAYPHRAANPVPVLVDILSALKARVLDDGYTGFQPSNLEITT
CCCCCCEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
IDVGNTATNVIPAAAKARVNIRFNPAHKGKDLAAWIEGECAKAAEGFDGAATALCKISGE
EECCCCHHHHCCCCCEEEEEEEECCCCCCCCEEEHHCCCHHHHHCCCCHHHHEEEEECCC
AFLTEPGDFTDVIVAAVTDATGRAPELSTTGGTSDARFIRALCPVVEFGLVGSTMHQVDE
EEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVPVEEVRQLAGAYEALIRRYFAAFA
CCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA