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The map label for this gene is def

Identifier: 16124527

GI number: 16124527

Start: 286774

End: 287295

Strand: Direct

Name: def

Synonym: CC_0272

Alternate gene names: 16124527

Gene position: 286774-287295 (Clockwise)

Preceding gene: 16124526

Following gene: 16124530

Centisome position: 7.14

GC content: 65.52

Gene sequence:

>522_bases
ATGGCTATCCGTCGCATCCTCACCGTCGATAACGCCGCCGACCTGGCGACGCTGAAGAAGATCTCTACGCCCGTGGAGGC
CGTCACCGATGAGCTGCGCGCTCTGATGGATGACATGTTGGAGACCATGTACGACGCCCCCGGCATCGGCCTGGCCGCCG
TTCAGGTCGGCGAGCCCGTGCGCGTGATCGTCATGGACCTGGCCCGCGAGGGCGAGGACAAGGCCCCGCGCTACTTCGTG
AACCCTGAGATCCTGGCCTCGTCCGAGGACCTGCAGGGCTATGAAGAAGGCTGCCTGTCGGTGCCCGAGTACTATGACGA
GGTCGAGCGCCCGTCCAAGGTCACCCTGCGCTATATGAACTACCAGGGCGAGACGGTCGTCGAGGAGGCCGAGGGCCTGT
TCGCCGTCTGCATCCAGCACGAGATGGACCACCTGGAAGGCGTGCTCTTCATCGACCACCTCTCGCGCCTGCGCCGCGAT
CGCGCCATGGCCAAGGTCAAGAAGGCGCGCCGGGCGGCTTAG

Upstream 100 bases:

>100_bases
CCATGGTCGATCAGGCGGTGCGTCCGTTGACCAAGCTGAAGGCCGTGGGACAATCCCCCACCTTGACGCTCGGAAATGAC
ACGCCTACCTGACGCTCATC

Downstream 100 bases:

>100_bases
GGGCTGCCGGCGCCTTAGATCCAACTGCCGCCGTCATCCCGCGCTTTATGCGCGGGACCCATCCGTCCGCCGCAGGTGCG
GAGCAGGATGGCGCTCTCAC

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MAIRRILTVDNAADLATLKKISTPVEAVTDELRALMDDMLETMYDAPGIGLAAVQVGEPVRVIVMDLAREGEDKAPRYFV
NPEILASSEDLQGYEEGCLSVPEYYDEVERPSKVTLRYMNYQGETVVEEAEGLFAVCIQHEMDHLEGVLFIDHLSRLRRD
RAMAKVKKARRAA

Sequences:

>Translated_173_residues
MAIRRILTVDNAADLATLKKISTPVEAVTDELRALMDDMLETMYDAPGIGLAAVQVGEPVRVIVMDLAREGEDKAPRYFV
NPEILASSEDLQGYEEGCLSVPEYYDEVERPSKVTLRYMNYQGETVVEEAEGLFAVCIQHEMDHLEGVLFIDHLSRLRRD
RAMAKVKKARRAA
>Mature_172_residues
AIRRILTVDNAADLATLKKISTPVEAVTDELRALMDDMLETMYDAPGIGLAAVQVGEPVRVIVMDLAREGEDKAPRYFVN
PEILASSEDLQGYEEGCLSVPEYYDEVERPSKVTLRYMNYQGETVVEEAEGLFAVCIQHEMDHLEGVLFIDHLSRLRRDR
AMAKVKKARRAA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=155, Percent_Identity=33.5483870967742, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1789682, Length=154, Percent_Identity=46.7532467532467, Blast_Score=133, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24645728, Length=146, Percent_Identity=33.5616438356164, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645726, Length=142, Percent_Identity=30.9859154929577, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CAUCN (B8GYE3)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002515648.1
- ProteinModelPortal:   B8GYE3
- SMR:   B8GYE3
- GeneID:   7330726
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_00273
- OMA:   MILPINI
- ProtClustDB:   PRK00150
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19446; Mature: 19314

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRRILTVDNAADLATLKKISTPVEAVTDELRALMDDMLETMYDAPGIGLAAVQVGEPV
CCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
RVIVMDLAREGEDKAPRYFVNPEILASSEDLQGYEEGCLSVPEYYDEVERPSKVTLRYMN
HHHHHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEC
YQGETVVEEAEGLFAVCIQHEMDHLEGVLFIDHLSRLRRDRAMAKVKKARRAA
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIRRILTVDNAADLATLKKISTPVEAVTDELRALMDDMLETMYDAPGIGLAAVQVGEPV
CCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
RVIVMDLAREGEDKAPRYFVNPEILASSEDLQGYEEGCLSVPEYYDEVERPSKVTLRYMN
HHHHHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEC
YQGETVVEEAEGLFAVCIQHEMDHLEGVLFIDHLSRLRRDRAMAKVKKARRAA
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA