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The map label for this gene is rlmN

Identifier: 16124389

GI number: 16124389

Start: 142680

End: 143894

Strand: Direct

Name: rlmN

Synonym: CC_0134

Alternate gene names: 16124389

Gene position: 142680-143894 (Clockwise)

Preceding gene: 16124388

Following gene: 16124390

Centisome position: 3.55

GC content: 67.74

Gene sequence:

>1215_bases
TTGAGCGTCACCCTCGACCTGTCGCGCGTCTCGTCCGACGCCGCGCCCGCCACCCCCGTCACCAAGCCCCTGATCAACCT
GTCGGGCCTGACGCGTCCGCAGCTGGTCGCGGCCCTGGTCGAGAGCGGCGTGGTCGAGCACGGCAAGGCCAAGATGCGGG
CCACCCAGATCTTCCGCTGGATGCACCATCGCGGCGTCACCGACTTCGCCGACATGAGCGACGTGGCCAAGGAAACCCGC
GCGCGTCTGGCTGAAGCCTTCACCATCGCCCGCCCCGAGATCGTCGAGCGCCAGGTGTCCAAGGACGGCACCCGCAAGTG
GCTGATCCGCATGGCCCCGGGCATCGAGGTCGAAAGCGTCTATATCCCCGGGGTCGGCCGCGCCGGCGCCCTGTGCGTGT
CCAGCCAGGTCGGCTGCACGCTGAACTGCAGCTTCTGCCACACCGGCACCCAGCCGCTGGTCCGCAACCTGACCGCGGCC
GAGATTGTCGCCCAGGTGCAGGTGGCCAAGGACGACCTGGCCGAATGGCCGTCCGACAAGGAAGACCGCCAGCTCTCGAA
CATCGTGTTCATGGGCATGGGCGAGCCGCTCTACAATCTGGGCCAGGTGGCCGACGCCATCGAGATCATCAGCGACAACG
AGGGCATCGCCATCTCGCGTCGCCGGATCACGGTCTCGACCTCGGGCGTGGTGCCGATGCTGGAAAAGCTGGGCTCGACC
ACCCAGGCCATGCTGGCGATCAGCCTGCACGCCACCAACGATCCGCTGCGCGACGTGCTGGTGCCGCTGAACAAGAAGTA
CCCGATCGCCGAGCTGATGGCCGGTATCCGCGCCTATCCGGGCCTGTCCAACGCCCGCCGCGTGACCTTCGAGTACGTGA
TGCTGAAGGGCGTCAACGACAGCCCCGAGGAAGCCCGCGCGCTCGTCAAGCTGATCAAGGGCATTCCGGCCAAGATCAAC
CTGATCCCGTTCAACCCCTGGCCCGGCAGCGATTACCAGTGCTCGGACTGGGCGACGATCGAGGCCTTCGCCGCGATCCT
CAACAAGGCCGGCTATTCCTCGCCGATCCGCACCCCGCGCGGCCGCGATATCCTCGCCGCCTGCGGCCAGCTGAAGAGCG
AGAGCGAGAAGGTCCGCGCCTCGGCCCTGCGCAAGCTGTCGCTGGCGGCGATGGCGGGCGTGCTGTCCGACGACGACGAG
GACGAGACGGCCTAA

Upstream 100 bases:

>100_bases
GCTGTGAGCTTGCAGGTCGGCCTGCGACCTGCGGTCCTTTTGGAAAACCGGGCCTGATCTGCTATAAGCCCGCCTCGACC
CCGAAGAGCCGGACATCCCC

Downstream 100 bases:

>100_bases
GGTCGCGTTCGCCGCGCACGGGCGCCCCCTACGCAAGCCTTGCTGACCCGTGTTAGTCAGGGGGCTGTAAGTCCACCAGG
GTTGATCCGCGTCCATGCCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSVTLDLSRVSSDAAPATPVTKPLINLSGLTRPQLVAALVESGVVEHGKAKMRATQIFRWMHHRGVTDFADMSDVAKETR
ARLAEAFTIARPEIVERQVSKDGTRKWLIRMAPGIEVESVYIPGVGRAGALCVSSQVGCTLNCSFCHTGTQPLVRNLTAA
EIVAQVQVAKDDLAEWPSDKEDRQLSNIVFMGMGEPLYNLGQVADAIEIISDNEGIAISRRRITVSTSGVVPMLEKLGST
TQAMLAISLHATNDPLRDVLVPLNKKYPIAELMAGIRAYPGLSNARRVTFEYVMLKGVNDSPEEARALVKLIKGIPAKIN
LIPFNPWPGSDYQCSDWATIEAFAAILNKAGYSSPIRTPRGRDILAACGQLKSESEKVRASALRKLSLAAMAGVLSDDDE
DETA

Sequences:

>Translated_404_residues
MSVTLDLSRVSSDAAPATPVTKPLINLSGLTRPQLVAALVESGVVEHGKAKMRATQIFRWMHHRGVTDFADMSDVAKETR
ARLAEAFTIARPEIVERQVSKDGTRKWLIRMAPGIEVESVYIPGVGRAGALCVSSQVGCTLNCSFCHTGTQPLVRNLTAA
EIVAQVQVAKDDLAEWPSDKEDRQLSNIVFMGMGEPLYNLGQVADAIEIISDNEGIAISRRRITVSTSGVVPMLEKLGST
TQAMLAISLHATNDPLRDVLVPLNKKYPIAELMAGIRAYPGLSNARRVTFEYVMLKGVNDSPEEARALVKLIKGIPAKIN
LIPFNPWPGSDYQCSDWATIEAFAAILNKAGYSSPIRTPRGRDILAACGQLKSESEKVRASALRKLSLAAMAGVLSDDDE
DETA
>Mature_403_residues
SVTLDLSRVSSDAAPATPVTKPLINLSGLTRPQLVAALVESGVVEHGKAKMRATQIFRWMHHRGVTDFADMSDVAKETRA
RLAEAFTIARPEIVERQVSKDGTRKWLIRMAPGIEVESVYIPGVGRAGALCVSSQVGCTLNCSFCHTGTQPLVRNLTAAE
IVAQVQVAKDDLAEWPSDKEDRQLSNIVFMGMGEPLYNLGQVADAIEIISDNEGIAISRRRITVSTSGVVPMLEKLGSTT
QAMLAISLHATNDPLRDVLVPLNKKYPIAELMAGIRAYPGLSNARRVTFEYVMLKGVNDSPEEARALVKLIKGIPAKINL
IPFNPWPGSDYQCSDWATIEAFAAILNKAGYSSPIRTPRGRDILAACGQLKSESEKVRASALRKLSLAAMAGVLSDDDED
ETA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=363, Percent_Identity=46.2809917355372, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CAUCN (B8GXM4)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002515508.1
- ProteinModelPortal:   B8GXM4
- GeneID:   7332387
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_00133
- OMA:   DINDQPW
- ProtClustDB:   CLSK889734
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 43737; Mature: 43606

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTLDLSRVSSDAAPATPVTKPLINLSGLTRPQLVAALVESGVVEHGKAKMRATQIFRW
CEEEEEHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCHHCCHHHHHHHHHHHH
MHHRGVTDFADMSDVAKETRARLAEAFTIARPEIVERQVSKDGTRKWLIRMAPGIEVESV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCEEEEE
YIPGVGRAGALCVSSQVGCTLNCSFCHTGTQPLVRNLTAAEIVAQVQVAKDDLAEWPSDK
EECCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EDRQLSNIVFMGMGEPLYNLGQVADAIEIISDNEGIAISRRRITVSTSGVVPMLEKLGST
HHHHHHCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHCCC
TQAMLAISLHATNDPLRDVLVPLNKKYPIAELMAGIRAYPGLSNARRVTFEYVMLKGVND
CEEEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCC
SPEEARALVKLIKGIPAKINLIPFNPWPGSDYQCSDWATIEAFAAILNKAGYSSPIRTPR
CHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
GRDILAACGQLKSESEKVRASALRKLSLAAMAGVLSDDDEDETA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVTLDLSRVSSDAAPATPVTKPLINLSGLTRPQLVAALVESGVVEHGKAKMRATQIFRW
EEEEEHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCHHCCHHHHHHHHHHHH
MHHRGVTDFADMSDVAKETRARLAEAFTIARPEIVERQVSKDGTRKWLIRMAPGIEVESV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCEEEEE
YIPGVGRAGALCVSSQVGCTLNCSFCHTGTQPLVRNLTAAEIVAQVQVAKDDLAEWPSDK
EECCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EDRQLSNIVFMGMGEPLYNLGQVADAIEIISDNEGIAISRRRITVSTSGVVPMLEKLGST
HHHHHHCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHCCC
TQAMLAISLHATNDPLRDVLVPLNKKYPIAELMAGIRAYPGLSNARRVTFEYVMLKGVND
CEEEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCC
SPEEARALVKLIKGIPAKINLIPFNPWPGSDYQCSDWATIEAFAAILNKAGYSSPIRTPR
CHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
GRDILAACGQLKSESEKVRASALRKLSLAAMAGVLSDDDEDETA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA