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The map label for this gene is phnH [H]

Identifier: 13488283

GI number: 13488283

Start: 254126

End: 254761

Strand: Direct

Name: phnH [H]

Synonym: mlr9279

Alternate gene names: 13488283

Gene position: 254126-254761 (Clockwise)

Preceding gene: 13488282

Following gene: 13488284

Centisome position: 72.21

GC content: 62.58

Gene sequence:

>636_bases
GTGACGGTAAAATCTTCAACATCATCTTTGCATGACCGATCCTCAACGAGCACTTCGCTGATGCCTGGCTTTGCCGATCC
GGTCTTCGATGCCCAGGCGGTGTTTCGCGCGAGCCTGCTTGCGACCGCCTATCCCGGCCGTGTCGTGCCAGTCGATCGCG
CATTGGCGTCGCCGCGGCCTTTTTCATCGGCAACCGCGGCACTGTGCCTGACGCTCATGGATTTCGAGACGCTGGCCTGG
CTCGACCGCCAGGCGGCATCCGGCGAGGCGGTTGCCTGGCTCCGCTTTCACTGCGGCCTGCCCGTCACAGACGATCCTAT
GAAGGCGCGTTTCGCTGTCGTGACCGATGCGCTGACAATGCCGCGCCTCGTCGAATTTCACCCAGGCGACGTGGAATACC
CTGATCGCTCGATGACGCTGATCATCCAGGTTCCGTCCTTCAACGAGGGGCCGGCGACCACCTGGACCGGCCCCGGCATC
AAGACGAGCGCTCGCGTAAGCATCGGCGGGTTGCCCTTCTGGTACTGGTCGGACTGGGAGATGAATAGAGAACTCTACCC
GCGCGGGATCGACGTCATCTTCACGAACGGCAATGCCCTGGTGGGCCTGCCGCGCACAATCGCGGTGGAGGCTTAA

Upstream 100 bases:

>100_bases
CGCTTCGGGAAAAAGCCGAAGTCCGGCGGCTCGAAAAAAGCCGCAAGGCGGCAGCCACCAAGGTCGAGTTCTTCACCATG
GTTCGCGGGGAGGACCCAGC

Downstream 100 bases:

>100_bases
ATGGCTTATGTTGCCGTCAAGGGTGGCGAACAGGCCATCCTCAACACCCTCAGGCTGATCTCGGAAACCCGTCGCGGCGA
TCCGGCCTTGGCCGAACTGA

Product: phosphonate metabolism protein PhnH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTVKSSTSSLHDRSSTSTSLMPGFADPVFDAQAVFRASLLATAYPGRVVPVDRALASPRPFSSATAALCLTLMDFETLAW
LDRQAASGEAVAWLRFHCGLPVTDDPMKARFAVVTDALTMPRLVEFHPGDVEYPDRSMTLIIQVPSFNEGPATTWTGPGI
KTSARVSIGGLPFWYWSDWEMNRELYPRGIDVIFTNGNALVGLPRTIAVEA

Sequences:

>Translated_211_residues
MTVKSSTSSLHDRSSTSTSLMPGFADPVFDAQAVFRASLLATAYPGRVVPVDRALASPRPFSSATAALCLTLMDFETLAW
LDRQAASGEAVAWLRFHCGLPVTDDPMKARFAVVTDALTMPRLVEFHPGDVEYPDRSMTLIIQVPSFNEGPATTWTGPGI
KTSARVSIGGLPFWYWSDWEMNRELYPRGIDVIFTNGNALVGLPRTIAVEA
>Mature_210_residues
TVKSSTSSLHDRSSTSTSLMPGFADPVFDAQAVFRASLLATAYPGRVVPVDRALASPRPFSSATAALCLTLMDFETLAWL
DRQAASGEAVAWLRFHCGLPVTDDPMKARFAVVTDALTMPRLVEFHPGDVEYPDRSMTLIIQVPSFNEGPATTWTGPGIK
TSARVSIGGLPFWYWSDWEMNRELYPRGIDVIFTNGNALVGLPRTIAVEA

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3625

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnH family [H]

Homologues:

Organism=Escherichia coli, GI1790538, Length=192, Percent_Identity=33.8541666666667, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008772 [H]

Pfam domain/function: PF05845 PhnH [H]

EC number: NA

Molecular weight: Translated: 22997; Mature: 22866

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKSSTSSLHDRSSTSTSLMPGFADPVFDAQAVFRASLLATAYPGRVVPVDRALASPRP
CCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCC
FSSATAALCLTLMDFETLAWLDRQAASGEAVAWLRFHCGLPVTDDPMKARFAVVTDALTM
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHEEEEEHHHHCC
PRLVEFHPGDVEYPDRSMTLIIQVPSFNEGPATTWTGPGIKTSARVSIGGLPFWYWSDWE
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCC
MNRELYPRGIDVIFTNGNALVGLPRTIAVEA
CCCEECCCCEEEEEECCCEEEECCCEEEECC
>Mature Secondary Structure 
TVKSSTSSLHDRSSTSTSLMPGFADPVFDAQAVFRASLLATAYPGRVVPVDRALASPRP
CCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCC
FSSATAALCLTLMDFETLAWLDRQAASGEAVAWLRFHCGLPVTDDPMKARFAVVTDALTM
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHEEEEEHHHHCC
PRLVEFHPGDVEYPDRSMTLIIQVPSFNEGPATTWTGPGIKTSARVSIGGLPFWYWSDWE
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCC
MNRELYPRGIDVIFTNGNALVGLPRTIAVEA
CCCEECCCCEEEEEECCCEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]