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The map label for this gene is phnI [H]

Identifier: 13488284

GI number: 13488284

Start: 254762

End: 255871

Strand: Direct

Name: phnI [H]

Synonym: mlr9280

Alternate gene names: 13488284

Gene position: 254762-255871 (Clockwise)

Preceding gene: 13488283

Following gene: 13488285

Centisome position: 72.39

GC content: 63.42

Gene sequence:

>1110_bases
ATGGCTTATGTTGCCGTCAAGGGTGGCGAACAGGCCATCCTCAACACCCTCAGGCTGATCTCGGAAACCCGTCGCGGCGA
TCCGGCCTTGGCCGAACTGACGCTCGCCCAGATCAGCGAACAGTTCGGCCTGGCCGTCGACCGGGTGATGACAGAGGGAT
CGGTCTACGACCGGGAACTCGCCGCACTTGCCCTCAAGCAGGCGCAGGGTGATGTGACCGAGGCGATCTTCCTGCTGCGC
GCCTACCGCACCACGCTGCCGCGCATCGCGCTGTCAGAACCGGTCGATACGTCGCGCATGACGATCCGCCGGCGCATCTC
GGCGACGTTCAAGGATATCCCGGGCGGCCAGGTGCTTGGGCCCACCTACGACTACACGCACCGGCTGCTCGATTTTGCCC
TCGCCGCCGAAGGCCTCCAGCGGCCCCAGGCAGGTTTGGCCGCCGAGCCGCTCGAACAAGATGTGCCGCAGGTCATCGAC
CTGCTCGACAGCGAAGGTCTGATCGAAGAGGAAAGCGACGAAGGTCAGGGCGAAATCTTCGATCTCACCCGCGAGCCGAT
GAGCTTTCCAGCAGGGCGCGACCAGCGCCTGCAGAACCTGGCGCGCGGCGACGAGGGTTTTCTGCTCGGGCTCGCTTATT
CGACAGTCCGTGGTTACGGCGGCGCGCATCCCTTCGTCGGCGAACTGAGATTGGGTGAAGTGACGGTCGAGATCGTCCCC
GAGGAGCTCGGTTTTCCGATTGTTGTCGCGGAAATCGCCGTCACCGAATGCCAGATGATCAACCAGTTTCGTGGCACGGT
TGCCCGACCGCCGCAGTTCACCCGCGGTTATGGCCTCACCTTCGGCCACAACGAGCGCAAGGCGATGGCCATGTCATTAG
TCGACCGGGCGATGCGCGCCCGCGAACTTGCCGAGGACGTACGCTACCCCGCCCAGGATGAGGAATTCGTCCTTGCCCAT
ACGGACAATGTCGAGACGAGCGGCATGGTCTCGCATTTCAAGCTGCCGCATTACGTCGATTTTCAGGCGGAACTGATGCT
TGTCCGACAACTGCGGGCCGAGCACCAGGTCGCCCGCGGGGCAGGACAGAAGAAGGAAGCCGCCGAATGA

Upstream 100 bases:

>100_bases
GGAGATGAATAGAGAACTCTACCCGCGCGGGATCGACGTCATCTTCACGAACGGCAATGCCCTGGTGGGCCTGCCGCGCA
CAATCGCGGTGGAGGCTTAA

Downstream 100 bases:

>100_bases
GCATTGGCACGACGACGAACCACAGCGATACAGGCTACAACTTCGCCTATCTCGATGAGCAGACGAAGCGGATGATCCGC
CGCTCGCTATTGAAGGCCCT

Product: phosphonate metabolism protein PhnI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MAYVAVKGGEQAILNTLRLISETRRGDPALAELTLAQISEQFGLAVDRVMTEGSVYDRELAALALKQAQGDVTEAIFLLR
AYRTTLPRIALSEPVDTSRMTIRRRISATFKDIPGGQVLGPTYDYTHRLLDFALAAEGLQRPQAGLAAEPLEQDVPQVID
LLDSEGLIEEESDEGQGEIFDLTREPMSFPAGRDQRLQNLARGDEGFLLGLAYSTVRGYGGAHPFVGELRLGEVTVEIVP
EELGFPIVVAEIAVTECQMINQFRGTVARPPQFTRGYGLTFGHNERKAMAMSLVDRAMRARELAEDVRYPAQDEEFVLAH
TDNVETSGMVSHFKLPHYVDFQAELMLVRQLRAEHQVARGAGQKKEAAE

Sequences:

>Translated_369_residues
MAYVAVKGGEQAILNTLRLISETRRGDPALAELTLAQISEQFGLAVDRVMTEGSVYDRELAALALKQAQGDVTEAIFLLR
AYRTTLPRIALSEPVDTSRMTIRRRISATFKDIPGGQVLGPTYDYTHRLLDFALAAEGLQRPQAGLAAEPLEQDVPQVID
LLDSEGLIEEESDEGQGEIFDLTREPMSFPAGRDQRLQNLARGDEGFLLGLAYSTVRGYGGAHPFVGELRLGEVTVEIVP
EELGFPIVVAEIAVTECQMINQFRGTVARPPQFTRGYGLTFGHNERKAMAMSLVDRAMRARELAEDVRYPAQDEEFVLAH
TDNVETSGMVSHFKLPHYVDFQAELMLVRQLRAEHQVARGAGQKKEAAE
>Mature_368_residues
AYVAVKGGEQAILNTLRLISETRRGDPALAELTLAQISEQFGLAVDRVMTEGSVYDRELAALALKQAQGDVTEAIFLLRA
YRTTLPRIALSEPVDTSRMTIRRRISATFKDIPGGQVLGPTYDYTHRLLDFALAAEGLQRPQAGLAAEPLEQDVPQVIDL
LDSEGLIEEESDEGQGEIFDLTREPMSFPAGRDQRLQNLARGDEGFLLGLAYSTVRGYGGAHPFVGELRLGEVTVEIVPE
ELGFPIVVAEIAVTECQMINQFRGTVARPPQFTRGYGLTFGHNERKAMAMSLVDRAMRARELAEDVRYPAQDEEFVLAHT
DNVETSGMVSHFKLPHYVDFQAELMLVRQLRAEHQVARGAGQKKEAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=354, Percent_Identity=61.2994350282486, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 40786; Mature: 40655

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYVAVKGGEQAILNTLRLISETRRGDPALAELTLAQISEQFGLAVDRVMTEGSVYDREL
CEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AALALKQAQGDVTEAIFLLRAYRTTLPRIALSEPVDTSRMTIRRRISATFKDIPGGQVLG
HHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEC
PTYDYTHRLLDFALAAEGLQRPQAGLAAEPLEQDVPQVIDLLDSEGLIEEESDEGQGEIF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
DLTREPMSFPAGRDQRLQNLARGDEGFLLGLAYSTVRGYGGAHPFVGELRLGEVTVEIVP
ECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCCCCEEECEEEEEEEH
EELGFPIVVAEIAVTECQMINQFRGTVARPPQFTRGYGLTFGHNERKAMAMSLVDRAMRA
HHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RELAEDVRYPAQDEEFVLAHTDNVETSGMVSHFKLPHYVDFQAELMLVRQLRAEHQVARG
HHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCEECHHHHHHHHHHHHHHHHHHHC
AGQKKEAAE
CCCCCCCCC
>Mature Secondary Structure 
AYVAVKGGEQAILNTLRLISETRRGDPALAELTLAQISEQFGLAVDRVMTEGSVYDREL
EEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AALALKQAQGDVTEAIFLLRAYRTTLPRIALSEPVDTSRMTIRRRISATFKDIPGGQVLG
HHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEC
PTYDYTHRLLDFALAAEGLQRPQAGLAAEPLEQDVPQVIDLLDSEGLIEEESDEGQGEIF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
DLTREPMSFPAGRDQRLQNLARGDEGFLLGLAYSTVRGYGGAHPFVGELRLGEVTVEIVP
ECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCCCCEEECEEEEEEEH
EELGFPIVVAEIAVTECQMINQFRGTVARPPQFTRGYGLTFGHNERKAMAMSLVDRAMRA
HHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RELAEDVRYPAQDEEFVLAHTDNVETSGMVSHFKLPHYVDFQAELMLVRQLRAEHQVARG
HHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCEECHHHHHHHHHHHHHHHHHHHC
AGQKKEAAE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]