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The map label for this gene is mcbR [H]

Identifier: 13488202

GI number: 13488202

Start: 45172

End: 45963

Strand: Direct

Name: mcbR [H]

Synonym: mlr9047

Alternate gene names: 13488202

Gene position: 45172-45963 (Clockwise)

Preceding gene: 13488344

Following gene: 13488203

Centisome position: 12.84

GC content: 53.03

Gene sequence:

>792_bases
GTGTTCACAGAGGCTTTCGAAGCACCCATTCCTGATGTCATCCCCAGTATTTGGAGATCATGCCAAATGGCGACAAAAAC
CTTGAAGTACCAGATACCACCATTGAACGCTTCCCCGAGTTCGGCGATTGAGCATGTCTATGCCGAACTGAAGAACGCTT
TGATGTCCGGGGAATTTTCGCCCGGGCAGCCGATGCGTCTCGGAGAACTGGCTGTCGCATTCGGCACAAGCCACATGCCG
ATCCGCGAGTCGCTCAACCGGCTCAGCGGCATCGACATCCTCGAACGGGCGCCGCGTCAGTCCGCGCGCGTTCCAATAAT
CACTGCTAAAGGCCTACGCGATCTTCTGGAAGTGCGCCTTCTGAATGAAAGGCAGGCCATCATCTGGGGGGCAAAGAAAT
CTACCGGGAAGAGCCTGAACTATATCAGAGAAATCAATGTCAAGATGGATCAACTCAATCTGGGGAAGAACGCTGAAGTG
AAGAAGTATCTGAAACTGAACCAACTGTTCCATTTTGCCGTTTACAACCTCTCTCAGAACAAAGTCTTGATGAACACGAT
CGAGACGCATTGGCTTCATGCGGGCCCGATCCTCAGCCTGAGGCGAAAGGAACCAAATTTTGCTCCGGGCCACCATAATC
ATCGCGAAATCATCGACCAGTTGGAGAAGGGCAACGGCTTGGCAGCCGCCGACGCCCTCGAGCGCAACATCATAGAAGCG
CATGAGCAGATTTTTGCGATTCTTGACAAGTCCGACCTGAAGGATCGTGCGGGAAATAGGGTCGGTGCCTAG

Upstream 100 bases:

>100_bases
GCTGTGCCACGCGTTTGCGATGCGGCCGCACTTCGTGCGTGGACCAGCGGACCAGGGTTCGGGTGGGCTGGTGCCCCGCC
GTTTTGATGCAGCGTGAAAC

Downstream 100 bases:

>100_bases
CTTAGGTAGGAACGCGGAAGCGTGCCTCGGGATGGTAACAGCTAGTATCCCTTGCTCGTCGAAGGCCATGCACGCAATTG
CGGCTGTCGAATGCGGCCGG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MFTEAFEAPIPDVIPSIWRSCQMATKTLKYQIPPLNASPSSAIEHVYAELKNALMSGEFSPGQPMRLGELAVAFGTSHMP
IRESLNRLSGIDILERAPRQSARVPIITAKGLRDLLEVRLLNERQAIIWGAKKSTGKSLNYIREINVKMDQLNLGKNAEV
KKYLKLNQLFHFAVYNLSQNKVLMNTIETHWLHAGPILSLRRKEPNFAPGHHNHREIIDQLEKGNGLAAADALERNIIEA
HEQIFAILDKSDLKDRAGNRVGA

Sequences:

>Translated_263_residues
MFTEAFEAPIPDVIPSIWRSCQMATKTLKYQIPPLNASPSSAIEHVYAELKNALMSGEFSPGQPMRLGELAVAFGTSHMP
IRESLNRLSGIDILERAPRQSARVPIITAKGLRDLLEVRLLNERQAIIWGAKKSTGKSLNYIREINVKMDQLNLGKNAEV
KKYLKLNQLFHFAVYNLSQNKVLMNTIETHWLHAGPILSLRRKEPNFAPGHHNHREIIDQLEKGNGLAAADALERNIIEA
HEQIFAILDKSDLKDRAGNRVGA
>Mature_263_residues
MFTEAFEAPIPDVIPSIWRSCQMATKTLKYQIPPLNASPSSAIEHVYAELKNALMSGEFSPGQPMRLGELAVAFGTSHMP
IRESLNRLSGIDILERAPRQSARVPIITAKGLRDLLEVRLLNERQAIIWGAKKSTGKSLNYIREINVKMDQLNLGKNAEV
KKYLKLNQLFHFAVYNLSQNKVLMNTIETHWLHAGPILSLRRKEPNFAPGHHNHREIIDQLEKGNGLAAADALERNIIEA
HEQIFAILDKSDLKDRAGNRVGA

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=152, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 29512; Mature: 29512

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTEAFEAPIPDVIPSIWRSCQMATKTLKYQIPPLNASPSSAIEHVYAELKNALMSGEFS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PGQPMRLGELAVAFGTSHMPIRESLNRLSGIDILERAPRQSARVPIITAKGLRDLLEVRL
CCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
LNERQAIIWGAKKSTGKSLNYIREINVKMDQLNLGKNAEVKKYLKLNQLFHFAVYNLSQN
HCCCEEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
KVLMNTIETHWLHAGPILSLRRKEPNFAPGHHNHREIIDQLEKGNGLAAADALERNIIEA
CHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
HEQIFAILDKSDLKDRAGNRVGA
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFTEAFEAPIPDVIPSIWRSCQMATKTLKYQIPPLNASPSSAIEHVYAELKNALMSGEFS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PGQPMRLGELAVAFGTSHMPIRESLNRLSGIDILERAPRQSARVPIITAKGLRDLLEVRL
CCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
LNERQAIIWGAKKSTGKSLNYIREINVKMDQLNLGKNAEVKKYLKLNQLFHFAVYNLSQN
HCCCEEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
KVLMNTIETHWLHAGPILSLRRKEPNFAPGHHNHREIIDQLEKGNGLAAADALERNIIEA
CHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
HEQIFAILDKSDLKDRAGNRVGA
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]