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The map label for this gene is grsB [H]

Identifier: 13488203

GI number: 13488203

Start: 46497

End: 48380

Strand: Direct

Name: grsB [H]

Synonym: mlr9052

Alternate gene names: 13488203

Gene position: 46497-48380 (Clockwise)

Preceding gene: 13488202

Following gene: 13488204

Centisome position: 13.21

GC content: 55.63

Gene sequence:

>1884_bases
GTGCTTTGGTATGCATCCGAATTTCCTCGTCGTCCTGCTGTCATTTTCGGAACAGAGGCGATCAACTACATGGATTTGTG
GCTGCGAGCCTGTGAGATAGCTCGTGCCCTAAAAACTGGACGTGAAGCGGGTTTGGACCGCGTGGCCCTTTATTTTCCCA
TCGGTATTGACCGGATCGCGGCGATCCTCTGCTGTCAAATCCTAGGTGTTTCCTACGTTCCTGTGGAACCAAATCTTCCA
GCGAGTCGCATTCGCGAGATGCTTGCGCAAGCGGATCCGAGCGTGCTCCTCAGTTCATCGGCCATCTGTTCGGCGATCCT
CAAGGAGGTCTCGTCGTGCCCGACAGTGATGGTCGAGGAAGTTGGAGTGGGCGCCGCTGATCGTGGATCGTTGAGCCGAG
ATTTGGCGCTGCCGCCGAAGTCCGGTGGCTACGTTATCTTTACCTCGGGTTCGACTGGCAAGCCGAAGGCAGTCAACATG
GGAGGAGCAGCACTTCAAAACCTCGTGGACTGGCAGATCGAACTTTCGACGCTGTCAGACAATGCGGCAACGGCTCAGTT
CGCCCCCATTTCGTTCGATGTATCGTTTCAGGAGATATTCTCCACGCTCTGTTCTGGTGGGAGTATCGTCCTCCTAGCGA
ATGAACAGCGGATCGACCCCGATTTGCTTTCGGACGAGATACTGCGTGCCCGGGTGGAGCGATTGTTTCTGCCATTTATC
GCTCTCCAACAATTGGCGTCGAATTGTGTGGAGCGCAATTTGTTTCCCGACAGTCTACGGGAGATCCACACCGCCGGCGA
ACAACTCGTGGTCTCTTCCGCTTTGCGCGAGTTTTTCATCAAGCTCCCCCAGTGTCGGCTCTTCAATCAATATGGTCCCT
CCGAGACACACGTCGTCACCTGTCACGAGCTTGATAGCAATCCAGCGGAATGGCCTAGGTTACCGCCTATCGGTCGGCCT
CTACCGAATGTCGTACTGTTCATTTTAGGTGAAGATGGCCGACCGGTGAGGTTAGGAGAGGTGGGAGAGTTGTATATTGG
TGGCGTCTGCCTCGCGCAGGGGTATTTCCAGGACAAGGAGCGAACAGACGAACGGTTCATGACTATAGATATTAACGGCA
CGCTAACGAGATTGTATCGGACCGGCGACTTTGCGACATCAGATGAAAGTGGTTGTTTTTTCTTCTGTGGTCGCCGAGAC
CACCAAATCAAGATCGATGGCTACAGGGTTGAACTTGGGGAAATTGAGAGCGTTATAGCGGATCACCCAGACGTAGCCGA
AGTCGCCGTGGTTTTCGATCGTGATGCAAATGGAACGGGACGACTGATCGCCTGCCTGACGAGCAAAGATGGGGCGCCAA
CCGACTGTCTCGAGACAGTAGTTCGCGCCCATGTCCGTGAGAAGCTCCCTGGCTACATGGCGCCAGACCGTGTCCAGATC
ATCGGCGCGATGCCTAAAACTGCGAGTGGCAAGGTCGATCGGAAATCAATCGCGGAAAGGCTGTGTGCCGAAAAGGTCAA
CCCCTCGGCACCGCCTGTCGAGAAGCCAGCCTGTGCCACTACGAGCGGATCGGTGAAGAACAGCGATCTGTGCGGCGCGA
TCACCTCTTACTGGCGGGAGCTCCTGGACCACCCCTTGCTCTCGGACTCGGATAATGTGTTCGATTTTGGCGCGCGTTCG
ATCATGGTCCCGGAGCTTCAACGCCGCCTTCGCCGTCAATTCGGGATTAGCATGCCCGCGATCTTGGTCTTCCGGCATCC
TTCGCCGAGGGAGCTTGCAGACTTTTTATCGCGAAGATCGGTGAAGGGCGATGCATTGAACACTACGTTGGCTGGTCTCG
CAAACCGGCGTGTGTCGACCAAAAGACGCGCTTCTCCGCGCTGA

Upstream 100 bases:

>100_bases
CCGAAGCAAAGCCGTTTGGCAGTGTCCTCGCATTCTGAGTGAAAAGATGCAGAGTTTAACAAGGGCCGCAGGTCCGTCAG
ATCGGACCATTGCAGCTTCC

Downstream 100 bases:

>100_bases
TCCTGGGCAGCGATGACCCCTCCAGCAGATCAGATTAGCGCGCTCCGGAAGCCAAAAAGCAGATAAACGGACCAGCGACG
AGGCAAAAATGAACGGCCCC

Product: peptide synthetase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: Gramicidin S synthase II; ATP-dependent proline adenylase; ProA; Proline activase; ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent ornithine adenylase; OrnA; Ornithine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MLWYASEFPRRPAVIFGTEAINYMDLWLRACEIARALKTGREAGLDRVALYFPIGIDRIAAILCCQILGVSYVPVEPNLP
ASRIREMLAQADPSVLLSSSAICSAILKEVSSCPTVMVEEVGVGAADRGSLSRDLALPPKSGGYVIFTSGSTGKPKAVNM
GGAALQNLVDWQIELSTLSDNAATAQFAPISFDVSFQEIFSTLCSGGSIVLLANEQRIDPDLLSDEILRARVERLFLPFI
ALQQLASNCVERNLFPDSLREIHTAGEQLVVSSALREFFIKLPQCRLFNQYGPSETHVVTCHELDSNPAEWPRLPPIGRP
LPNVVLFILGEDGRPVRLGEVGELYIGGVCLAQGYFQDKERTDERFMTIDINGTLTRLYRTGDFATSDESGCFFFCGRRD
HQIKIDGYRVELGEIESVIADHPDVAEVAVVFDRDANGTGRLIACLTSKDGAPTDCLETVVRAHVREKLPGYMAPDRVQI
IGAMPKTASGKVDRKSIAERLCAEKVNPSAPPVEKPACATTSGSVKNSDLCGAITSYWRELLDHPLLSDSDNVFDFGARS
IMVPELQRRLRRQFGISMPAILVFRHPSPRELADFLSRRSVKGDALNTTLAGLANRRVSTKRRASPR

Sequences:

>Translated_627_residues
MLWYASEFPRRPAVIFGTEAINYMDLWLRACEIARALKTGREAGLDRVALYFPIGIDRIAAILCCQILGVSYVPVEPNLP
ASRIREMLAQADPSVLLSSSAICSAILKEVSSCPTVMVEEVGVGAADRGSLSRDLALPPKSGGYVIFTSGSTGKPKAVNM
GGAALQNLVDWQIELSTLSDNAATAQFAPISFDVSFQEIFSTLCSGGSIVLLANEQRIDPDLLSDEILRARVERLFLPFI
ALQQLASNCVERNLFPDSLREIHTAGEQLVVSSALREFFIKLPQCRLFNQYGPSETHVVTCHELDSNPAEWPRLPPIGRP
LPNVVLFILGEDGRPVRLGEVGELYIGGVCLAQGYFQDKERTDERFMTIDINGTLTRLYRTGDFATSDESGCFFFCGRRD
HQIKIDGYRVELGEIESVIADHPDVAEVAVVFDRDANGTGRLIACLTSKDGAPTDCLETVVRAHVREKLPGYMAPDRVQI
IGAMPKTASGKVDRKSIAERLCAEKVNPSAPPVEKPACATTSGSVKNSDLCGAITSYWRELLDHPLLSDSDNVFDFGARS
IMVPELQRRLRRQFGISMPAILVFRHPSPRELADFLSRRSVKGDALNTTLAGLANRRVSTKRRASPR
>Mature_627_residues
MLWYASEFPRRPAVIFGTEAINYMDLWLRACEIARALKTGREAGLDRVALYFPIGIDRIAAILCCQILGVSYVPVEPNLP
ASRIREMLAQADPSVLLSSSAICSAILKEVSSCPTVMVEEVGVGAADRGSLSRDLALPPKSGGYVIFTSGSTGKPKAVNM
GGAALQNLVDWQIELSTLSDNAATAQFAPISFDVSFQEIFSTLCSGGSIVLLANEQRIDPDLLSDEILRARVERLFLPFI
ALQQLASNCVERNLFPDSLREIHTAGEQLVVSSALREFFIKLPQCRLFNQYGPSETHVVTCHELDSNPAEWPRLPPIGRP
LPNVVLFILGEDGRPVRLGEVGELYIGGVCLAQGYFQDKERTDERFMTIDINGTLTRLYRTGDFATSDESGCFFFCGRRD
HQIKIDGYRVELGEIESVIADHPDVAEVAVVFDRDANGTGRLIACLTSKDGAPTDCLETVVRAHVREKLPGYMAPDRVQI
IGAMPKTASGKVDRKSIAERLCAEKVNPSAPPVEKPACATTSGSVKNSDLCGAITSYWRELLDHPLLSDSDNVFDFGARS
IMVPELQRRLRRQFGISMPAILVFRHPSPRELADFLSRRSVKGDALNTTLAGLANRRVSTKRRASPR

Specific function: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Pro, Val, Orn and Leu. Activation sites for these AA consist of individual domains [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI28416953, Length=536, Percent_Identity=23.5074626865672, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI45580730, Length=453, Percent_Identity=24.0618101545254, Blast_Score=100, Evalue=8e-21,
Organism=Homo sapiens, GI115511026, Length=478, Percent_Identity=23.0125523012552, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI38505220, Length=474, Percent_Identity=22.9957805907173, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI156151445, Length=228, Percent_Identity=28.5087719298246, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI122937307, Length=487, Percent_Identity=22.9979466119097, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1786801, Length=595, Percent_Identity=29.9159663865546, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1790505, Length=530, Percent_Identity=24.1509433962264, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788107, Length=237, Percent_Identity=29.957805907173, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786810, Length=377, Percent_Identity=25.1989389920424, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786529, Length=524, Percent_Identity=25, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI221142682, Length=232, Percent_Identity=27.1551724137931, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=392, Percent_Identity=26.530612244898, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17556356, Length=458, Percent_Identity=24.4541484716157, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17550940, Length=504, Percent_Identity=24.2063492063492, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=492, Percent_Identity=25.2032520325203, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=388, Percent_Identity=25.5154639175258, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=376, Percent_Identity=24.468085106383, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=695, Percent_Identity=23.4532374100719, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=567, Percent_Identity=23.2804232804233, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=400, Percent_Identity=25.5, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648676, Length=460, Percent_Identity=29.5652173913043, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI62472339, Length=537, Percent_Identity=23.0912476722533, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24667955, Length=537, Percent_Identity=23.0912476722533, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24653035, Length=372, Percent_Identity=26.0752688172043, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355225, Length=381, Percent_Identity=26.509186351706, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24667959, Length=369, Percent_Identity=25.4742547425474, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI161076582, Length=379, Percent_Identity=24.802110817942, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130357, Length=232, Percent_Identity=29.7413793103448, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24581924, Length=377, Percent_Identity=23.6074270557029, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI18859661, Length=176, Percent_Identity=28.4090909090909, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68790; Mature: 68790

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWYASEFPRRPAVIFGTEAINYMDLWLRACEIARALKTGREAGLDRVALYFPIGIDRIA
CCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
AILCCQILGVSYVPVEPNLPASRIREMLAQADPSVLLSSSAICSAILKEVSSCPTVMVEE
HHHHHHHHCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEHHH
VGVGAADRGSLSRDLALPPKSGGYVIFTSGSTGKPKAVNMGGAALQNLVDWQIELSTLSD
HCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHHCEEEEEEECC
NAATAQFAPISFDVSFQEIFSTLCSGGSIVLLANEQRIDPDLLSDEILRARVERLFLPFI
CCCCEEECCEEECCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ALQQLASNCVERNLFPDSLREIHTAGEQLVVSSALREFFIKLPQCRLFNQYGPSETHVVT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEE
CHELDSNPAEWPRLPPIGRPLPNVVLFILGEDGRPVRLGEVGELYIGGVCLAQGYFQDKE
EEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEECHHHHHCCHHCCCC
RTDERFMTIDINGTLTRLYRTGDFATSDESGCFFFCGRRDHQIKIDGYRVELGEIESVIA
CCCCEEEEEECCCEEEEEEECCCCCCCCCCCEEEEECCCCCEEEEECEEEEHHHHHHHHC
DHPDVAEVAVVFDRDANGTGRLIACLTSKDGAPTDCLETVVRAHVREKLPGYMAPDRVQI
CCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEE
IGAMPKTASGKVDRKSIAERLCAEKVNPSAPPVEKPACATTSGSVKNSDLCGAITSYWRE
EECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LLDHPLLSDSDNVFDFGARSIMVPELQRRLRRQFGISMPAILVFRHPSPRELADFLSRRS
HHCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCC
VKGDALNTTLAGLANRRVSTKRRASPR
CCCCHHHHHHHHHHHCCCCHHHCCCCC
>Mature Secondary Structure
MLWYASEFPRRPAVIFGTEAINYMDLWLRACEIARALKTGREAGLDRVALYFPIGIDRIA
CCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
AILCCQILGVSYVPVEPNLPASRIREMLAQADPSVLLSSSAICSAILKEVSSCPTVMVEE
HHHHHHHHCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEHHH
VGVGAADRGSLSRDLALPPKSGGYVIFTSGSTGKPKAVNMGGAALQNLVDWQIELSTLSD
HCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHHCEEEEEEECC
NAATAQFAPISFDVSFQEIFSTLCSGGSIVLLANEQRIDPDLLSDEILRARVERLFLPFI
CCCCEEECCEEECCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ALQQLASNCVERNLFPDSLREIHTAGEQLVVSSALREFFIKLPQCRLFNQYGPSETHVVT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEE
CHELDSNPAEWPRLPPIGRPLPNVVLFILGEDGRPVRLGEVGELYIGGVCLAQGYFQDKE
EEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEECHHHHHCCHHCCCC
RTDERFMTIDINGTLTRLYRTGDFATSDESGCFFFCGRRDHQIKIDGYRVELGEIESVIA
CCCCEEEEEECCCEEEEEEECCCCCCCCCCCEEEEECCCCCEEEEECEEEEHHHHHHHHC
DHPDVAEVAVVFDRDANGTGRLIACLTSKDGAPTDCLETVVRAHVREKLPGYMAPDRVQI
CCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEE
IGAMPKTASGKVDRKSIAERLCAEKVNPSAPPVEKPACATTSGSVKNSDLCGAITSYWRE
EECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LLDHPLLSDSDNVFDFGARSIMVPELQRRLRRQFGISMPAILVFRHPSPRELADFLSRRS
HHCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCC
VKGDALNTTLAGLANRRVSTKRRASPR
CCCCHHHHHHHHHHHCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1560782; 2477357 [H]