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The map label for this gene is afaC [H]

Identifier: 13476410

GI number: 13476410

Start: 6395205

End: 6397628

Strand: Reverse

Name: afaC [H]

Synonym: mll7724

Alternate gene names: 13476410

Gene position: 6397628-6395205 (Counterclockwise)

Preceding gene: 13476411

Following gene: 13476409

Centisome position: 90.93

GC content: 61.22

Gene sequence:

>2424_bases
ATGCCAACGTTTCGATCAAGGGCCAGTAGGGTCGCGATCACCGCTGTCGTGACCGCATTTTCCACCATGCAGGTCAAAGC
ACAAGATGTGTTGCCGGCCCCCGCGGCAACGGATTCCGCGGCGGCTCCAGCCGACGGCCGCGATCTGTATCTGGAAGTCT
TCGTCAACGACGTCTCCACCGGGCTGATCGGCGCTTTCAGGCAGCTGTCGGATGGCGGCCTGGCTTCCACGCCAGACGAA
TTGCAGCAGGTTGGGCTGCAACCTGTCGAGGGTGCGATGGGTGCCGATCACCTGGTGCGGGTCGATCGGCTCCCGGACGT
CTCCTATCGTATCGATGAAGCGACCCAACGTCTCTATGTGACGGCCACCAATGCGGCACGCTCCGCCCACGTCATCGACA
TCGCTCCGCAGGGTAAGCAGGACAGGCTCAAGCCGCAATCCGGCTTTGGCGGCGTGCTGAACTATTCCCTGTTCGCCAGC
ATCAACGATCTGAAGGATCGTACCTCGCAGCTCTTTAGTGGCGTCTCAGGCGGTTTCGATGCGCGGCTTTTCAGCCCTTA
CGGAACGTTGAGCCAAGGCTTTATCGTCAGCCTGTCCGACGGCGAACTTGACGGCTTCACCCGGCTGAACACGACCTGGA
GTTATTCCGATCCCGAGCGGTTGCTGACCTACAAGGCGGGTGACTTCGTCTCGGGTGGGCTGGCATGGACAAGGTCCGTG
TATCTCGGCGGCTTTCAGGTCCAGCGCAATTTTTCCTTGCGTTCCGATCTCGTCACCTTGCCGCTTCCGAACTTCTCGGG
AACAGCTGCTGTCCCCTCCACGCTCGAAGTCTATACACAGAATGTGCGGACCTATACCAGCGACGTCCCGGCCGGGCCGT
TCCAGGTGACAAACCTGCCAATATTCACCGGAGCTGGCCAAGCCCAAATTGTCCTGCGCGATAGCCTCGGCCGCGAAACC
ACCGTGACGTTGCCGTTCTACAATTCCAGCATGCTTTTGAAACGAGGCCTGCTCGATTTCTCCGCCGAGGTCGGCTCTCC
GCGGCGCAATTTCGGTGTCGAATCGTATGATTATGACGGCGGCATCTATGGCTCGGCAAGTGCACGCTATGGGCTGACCA
ACCGGCTGACACTGGAAGGCCACGTCGAAGGCGGCGAAGATCTTGTGAATGGCGGCGCCGGCATAGCGTTTCCGCTCAGC
TATTTCGGCGCAGCCTCCATTGCTGCGGCCGGCAGTCGTCACAATGGCCAGACGGGCGCGCTCGTCAACGCCTCGCTTGA
GCTGAGCTACGATGGGTGGTCGCTCTATGGCCGCATGCAGCGCGCGTTCGCGGACTACGAGGACATTGCATCGGTCACCG
CCGAGATCAATGCTGTACCGGGTCCGAACGAGTTCCCGATCTTCACAGCCGGCGTTCCTCGCGCGCTCGACCAGGTAACG
CTGAGCGTGCCGATGCCGCTTGATTTTTCAAACCTCAACCTCTCCTACGCGCATCTTACGAGCGCCGAGGGAGAGAGCAG
CCAGATCGTCGGCGTTTCGTACAGCCAGACAGTGTTCAAGGACAGCAGCTTCTATGCCACCGCCTTCACCGACCTTGACG
ACAAAAACAGCTTTGGAGTGTTTGCGGGCATCTCCGTGCCACTCGGCGGTGATGTCACCGCCTCGACCGGGGTCGCAGAC
GGCCCTGACGGCGTGTCGGTGGTCGCCGACATCGCCAAGTCCGAACGGCTGGAGAACGGCAGCATCGGCTGGCGTGCGAG
GACGTCCGAGGGCGGCACGCCCAACCGGTCGGCAGCCGTCAGCTACCGTTCACCACGGGCACGAGTTGAGACCGGCATCC
AGCAATTCGACAAGAGCGTTCGTGGAACCGCAGAGGTCGATGGGGCAATCGCCGTCGCCGGCGGCGGCATTTTTGCCACC
AACCGCATCGACGACGCGTTTGCTGTTGTCGATGTCGGCGTTGCTGGCGTGGAGGTCCAGCACCAGAACAGGCCGGTCGG
CGTCAGCAACCGGCAGGGAAGGATCCTGGTTCCCGACCTGAACTCCTACGAAGACAATACCATTTCCATTGATCCAAGGA
ACTTGCCGGTCGATGCCGACGTGCCGGAGACCCGCAAAATCGTCGTGCCGGCCGGTCGAAGTGGCGTCGTCCTTGATTTC
GGCGTTTCAAAGGCTGCCGATGCGGCGCTTGTCTCCCTGGTCGACGGCAAAGGCACGCCGATCGCCGTCGGCGCCAAAGG
TCGTGTCGAGGGTGCCGACGAGGAATTCGTGATCGGCTATGACGGCGAGGCCTACATCCGCACCCTCCACCGACAAAATG
CCGTGGCCATTGAATTGCCGGACGGCACCTCGTGTCGAGCAGAATTCGCCTATCGAGCGATACCCGGCCAGCAGGTGGTC
ATCAAGCAGGTCATCTGTTCTTGA

Upstream 100 bases:

>100_bases
TATGTGCTCGGCGGTGCGACCATGCAATGGCCGATCGCGGGGAAGACGCTTTCGGGTGGGGCGGTTTTGCTGTCGGCCCA
GAGCGATTTTGGACCGTTCG

Downstream 100 bases:

>100_bases
GGCGAGGACGAACCATGCTCCGTTTCGCTATGCTGTCGGCCGCCCTGCTGTTGCCGACCGTTGCCTGGGCCCAAAGCTGC
AGCTTTGGCGTATCGGCGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MPTFRSRASRVAITAVVTAFSTMQVKAQDVLPAPAATDSAAAPADGRDLYLEVFVNDVSTGLIGAFRQLSDGGLASTPDE
LQQVGLQPVEGAMGADHLVRVDRLPDVSYRIDEATQRLYVTATNAARSAHVIDIAPQGKQDRLKPQSGFGGVLNYSLFAS
INDLKDRTSQLFSGVSGGFDARLFSPYGTLSQGFIVSLSDGELDGFTRLNTTWSYSDPERLLTYKAGDFVSGGLAWTRSV
YLGGFQVQRNFSLRSDLVTLPLPNFSGTAAVPSTLEVYTQNVRTYTSDVPAGPFQVTNLPIFTGAGQAQIVLRDSLGRET
TVTLPFYNSSMLLKRGLLDFSAEVGSPRRNFGVESYDYDGGIYGSASARYGLTNRLTLEGHVEGGEDLVNGGAGIAFPLS
YFGAASIAAAGSRHNGQTGALVNASLELSYDGWSLYGRMQRAFADYEDIASVTAEINAVPGPNEFPIFTAGVPRALDQVT
LSVPMPLDFSNLNLSYAHLTSAEGESSQIVGVSYSQTVFKDSSFYATAFTDLDDKNSFGVFAGISVPLGGDVTASTGVAD
GPDGVSVVADIAKSERLENGSIGWRARTSEGGTPNRSAAVSYRSPRARVETGIQQFDKSVRGTAEVDGAIAVAGGGIFAT
NRIDDAFAVVDVGVAGVEVQHQNRPVGVSNRQGRILVPDLNSYEDNTISIDPRNLPVDADVPETRKIVVPAGRSGVVLDF
GVSKAADAALVSLVDGKGTPIAVGAKGRVEGADEEFVIGYDGEAYIRTLHRQNAVAIELPDGTSCRAEFAYRAIPGQQVV
IKQVICS

Sequences:

>Translated_807_residues
MPTFRSRASRVAITAVVTAFSTMQVKAQDVLPAPAATDSAAAPADGRDLYLEVFVNDVSTGLIGAFRQLSDGGLASTPDE
LQQVGLQPVEGAMGADHLVRVDRLPDVSYRIDEATQRLYVTATNAARSAHVIDIAPQGKQDRLKPQSGFGGVLNYSLFAS
INDLKDRTSQLFSGVSGGFDARLFSPYGTLSQGFIVSLSDGELDGFTRLNTTWSYSDPERLLTYKAGDFVSGGLAWTRSV
YLGGFQVQRNFSLRSDLVTLPLPNFSGTAAVPSTLEVYTQNVRTYTSDVPAGPFQVTNLPIFTGAGQAQIVLRDSLGRET
TVTLPFYNSSMLLKRGLLDFSAEVGSPRRNFGVESYDYDGGIYGSASARYGLTNRLTLEGHVEGGEDLVNGGAGIAFPLS
YFGAASIAAAGSRHNGQTGALVNASLELSYDGWSLYGRMQRAFADYEDIASVTAEINAVPGPNEFPIFTAGVPRALDQVT
LSVPMPLDFSNLNLSYAHLTSAEGESSQIVGVSYSQTVFKDSSFYATAFTDLDDKNSFGVFAGISVPLGGDVTASTGVAD
GPDGVSVVADIAKSERLENGSIGWRARTSEGGTPNRSAAVSYRSPRARVETGIQQFDKSVRGTAEVDGAIAVAGGGIFAT
NRIDDAFAVVDVGVAGVEVQHQNRPVGVSNRQGRILVPDLNSYEDNTISIDPRNLPVDADVPETRKIVVPAGRSGVVLDF
GVSKAADAALVSLVDGKGTPIAVGAKGRVEGADEEFVIGYDGEAYIRTLHRQNAVAIELPDGTSCRAEFAYRAIPGQQVV
IKQVICS
>Mature_806_residues
PTFRSRASRVAITAVVTAFSTMQVKAQDVLPAPAATDSAAAPADGRDLYLEVFVNDVSTGLIGAFRQLSDGGLASTPDEL
QQVGLQPVEGAMGADHLVRVDRLPDVSYRIDEATQRLYVTATNAARSAHVIDIAPQGKQDRLKPQSGFGGVLNYSLFASI
NDLKDRTSQLFSGVSGGFDARLFSPYGTLSQGFIVSLSDGELDGFTRLNTTWSYSDPERLLTYKAGDFVSGGLAWTRSVY
LGGFQVQRNFSLRSDLVTLPLPNFSGTAAVPSTLEVYTQNVRTYTSDVPAGPFQVTNLPIFTGAGQAQIVLRDSLGRETT
VTLPFYNSSMLLKRGLLDFSAEVGSPRRNFGVESYDYDGGIYGSASARYGLTNRLTLEGHVEGGEDLVNGGAGIAFPLSY
FGAASIAAAGSRHNGQTGALVNASLELSYDGWSLYGRMQRAFADYEDIASVTAEINAVPGPNEFPIFTAGVPRALDQVTL
SVPMPLDFSNLNLSYAHLTSAEGESSQIVGVSYSQTVFKDSSFYATAFTDLDDKNSFGVFAGISVPLGGDVTASTGVADG
PDGVSVVADIAKSERLENGSIGWRARTSEGGTPNRSAAVSYRSPRARVETGIQQFDKSVRGTAEVDGAIAVAGGGIFATN
RIDDAFAVVDVGVAGVEVQHQNRPVGVSNRQGRILVPDLNSYEDNTISIDPRNLPVDADVPETRKIVVPAGRSGVVLDFG
VSKAADAALVSLVDGKGTPIAVGAKGRVEGADEEFVIGYDGEAYIRTLHRQNAVAIELPDGTSCRAEFAYRAIPGQQVVI
KQVICS

Specific function: Involved in the export and assembly of AFA-III afimbrial adhesin subunits across the outer membrane [H]

COG id: COG3188

COG function: function code NU; P pilus assembly protein, porin PapC

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fimbrial export usher family [H]

Homologues:

Organism=Escherichia coli, GI1789533, Length=272, Percent_Identity=25, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1786332, Length=620, Percent_Identity=22.741935483871, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000015
- InterPro:   IPR018030 [H]

Pfam domain/function: PF00577 Usher [H]

EC number: NA

Molecular weight: Translated: 85411; Mature: 85280

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTFRSRASRVAITAVVTAFSTMQVKAQDVLPAPAATDSAAAPADGRDLYLEVFVNDVST
CCCCHHHHHHEEEEEHHHHHHHEEEEHHHCCCCCCCCCCCCCCCCCCEEEEEEEECHHHH
GLIGAFRQLSDGGLASTPDELQQVGLQPVEGAMGADHLVRVDRLPDVSYRIDEATQRLYV
HHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCEEEEE
TATNAARSAHVIDIAPQGKQDRLKPQSGFGGVLNYSLFASINDLKDRTSQLFSGVSGGFD
EEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCC
ARLFSPYGTLSQGFIVSLSDGELDGFTRLNTTWSYSDPERLLTYKAGDFVSGGLAWTRSV
EEEECCCCCCCCCEEEEECCCCCCCEEEECCEECCCCHHHEEEEECCCCCCCCHHEEEEE
YLGGFQVQRNFSLRSDLVTLPLPNFSGTAAVPSTLEVYTQNVRTYTSDVPAGPFQVTNLP
EECCEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCEEEECEE
IFTGAGQAQIVLRDSLGRETTVTLPFYNSSMLLKRGLLDFSAEVGSPRRNFGVESYDYDG
EEECCCCEEEEEECCCCCCEEEEEEEECCHHHHHHCCHHHHHHCCCCHHHCCCCCCCCCC
GIYGSASARYGLTNRLTLEGHVEGGEDLVNGGAGIAFPLSYFGAASIAAAGSRHNGQTGA
CEEECCCCCCCCCEEEEEEEECCCCHHHHCCCCCEEEHHHHHCHHHHHHCCCCCCCCCCE
LVNASLELSYDGWSLYGRMQRAFADYEDIASVTAEINAVPGPNEFPIFTAGVPRALDQVT
EEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCCEEECCCCCCHHHEE
LSVPMPLDFSNLNLSYAHLTSAEGESSQIVGVSYSQTVFKDSSFYATAFTDLDDKNSFGV
EEECCCCCCCCCCEEEEEEECCCCCCCEEEEEECCHHEECCCCEEEEEEECCCCCCCCEE
FAGISVPLGGDVTASTGVADGPDGVSVVADIAKSERLENGSIGWRARTSEGGTPNRSAAV
EEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEE
SYRSPRARVETGIQQFDKSVRGTAEVDGAIAVAGGGIFATNRIDDAFAVVDVGVAGVEVQ
EECCCHHHHHHHHHHHHHHHCCCEECCCEEEEECCCEEEECCCCCEEEEEEECEEEEEEE
HQNRPVGVSNRQGRILVPDLNSYEDNTISIDPRNLPVDADVPETRKIVVPAGRSGVVLDF
CCCCCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEEC
GVSKAADAALVSLVDGKGTPIAVGAKGRVEGADEEFVIGYDGEAYIRTLHRQNAVAIELP
CCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHCCCEEEEECC
DGTSCRAEFAYRAIPGQQVVIKQVICS
CCCCCHHEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure 
PTFRSRASRVAITAVVTAFSTMQVKAQDVLPAPAATDSAAAPADGRDLYLEVFVNDVST
CCCHHHHHHEEEEEHHHHHHHEEEEHHHCCCCCCCCCCCCCCCCCCEEEEEEEECHHHH
GLIGAFRQLSDGGLASTPDELQQVGLQPVEGAMGADHLVRVDRLPDVSYRIDEATQRLYV
HHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCEEEEE
TATNAARSAHVIDIAPQGKQDRLKPQSGFGGVLNYSLFASINDLKDRTSQLFSGVSGGFD
EEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCC
ARLFSPYGTLSQGFIVSLSDGELDGFTRLNTTWSYSDPERLLTYKAGDFVSGGLAWTRSV
EEEECCCCCCCCCEEEEECCCCCCCEEEECCEECCCCHHHEEEEECCCCCCCCHHEEEEE
YLGGFQVQRNFSLRSDLVTLPLPNFSGTAAVPSTLEVYTQNVRTYTSDVPAGPFQVTNLP
EECCEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCEEEECEE
IFTGAGQAQIVLRDSLGRETTVTLPFYNSSMLLKRGLLDFSAEVGSPRRNFGVESYDYDG
EEECCCCEEEEEECCCCCCEEEEEEEECCHHHHHHCCHHHHHHCCCCHHHCCCCCCCCCC
GIYGSASARYGLTNRLTLEGHVEGGEDLVNGGAGIAFPLSYFGAASIAAAGSRHNGQTGA
CEEECCCCCCCCCEEEEEEEECCCCHHHHCCCCCEEEHHHHHCHHHHHHCCCCCCCCCCE
LVNASLELSYDGWSLYGRMQRAFADYEDIASVTAEINAVPGPNEFPIFTAGVPRALDQVT
EEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCCEEECCCCCCHHHEE
LSVPMPLDFSNLNLSYAHLTSAEGESSQIVGVSYSQTVFKDSSFYATAFTDLDDKNSFGV
EEECCCCCCCCCCEEEEEEECCCCCCCEEEEEECCHHEECCCCEEEEEEECCCCCCCCEE
FAGISVPLGGDVTASTGVADGPDGVSVVADIAKSERLENGSIGWRARTSEGGTPNRSAAV
EEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEE
SYRSPRARVETGIQQFDKSVRGTAEVDGAIAVAGGGIFATNRIDDAFAVVDVGVAGVEVQ
EECCCHHHHHHHHHHHHHHHCCCEECCCEEEEECCCEEEECCCCCEEEEEEECEEEEEEE
HQNRPVGVSNRQGRILVPDLNSYEDNTISIDPRNLPVDADVPETRKIVVPAGRSGVVLDF
CCCCCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEEC
GVSKAADAALVSLVDGKGTPIAVGAKGRVEGADEEFVIGYDGEAYIRTLHRQNAVAIELP
CCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHCCCEEEEECC
DGTSCRAEFAYRAIPGQQVVIKQVICS
CCCCCHHEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002584 [H]