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The map label for this gene is ccmE

Identifier: 13476385

GI number: 13476385

Start: 6364201

End: 6364644

Strand: Direct

Name: ccmE

Synonym: mlr7689

Alternate gene names: 13476385

Gene position: 6364201-6364644 (Clockwise)

Preceding gene: 13476384

Following gene: 13476386

Centisome position: 90.45

GC content: 63.06

Gene sequence:

>444_bases
ATGACGCGCAAGCAGAAACGACTGTCGGTCATTGTCGGCGGATTGGCTTTCCTTGGTGCCGCCACCGGGCTGACCTTCTA
CGCGCTCGGCCAGAAGGCTTCCTATTTCTACATGCCGGCCGACCTCACCACGGCGAGCGTCCAGCCCGGCCAGCGCATCA
GGCTCGGCGGTTTGGTCGAAAAAGGCACCATCCAGCGCGGGCAGGGCGCGACGGTCGCCTTTTCGGTCACCGACACGCAC
AAATCGGTCAAGGTCACCTATACCGGCATCCTGCCCGACCTCTTTCGCGAGGAGCAGGGCGTCATCACCGAGGGCAGTTT
CGGCCCGGACGGCGTCTTTGTTGCCGACAGCGTGCTGGCCAAGCATGATGAGCGCTACATGCCCAAGGAAGTGGCCGACG
GGCTGAAGGCCAAGGGCGTCTGGCAGGAGAGCAAAAGTGAGTAG

Upstream 100 bases:

>100_bases
GTCGCGCCATCGCCGTCTTTGGCGCGGACAGCGACGAAGCCAAGAAATTCACCGCCTTCGCCGCCTCGCTCGGCCTGACG
GCGACGGAGTAGACCTGGAT

Downstream 100 bases:

>100_bases
GTCTGGCAGAAGGGCAGGGGTCAATCATGGGCACAACCATGGTTGAAACCGGACATTTCGCCCTGGTCCTGGCGTTCGCG
CTTTCGCTGGTGCAGACGAT

Product: cytochrome c-type biogenesis protein CcmE

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MTRKQKRLSVIVGGLAFLGAATGLTFYALGQKASYFYMPADLTTASVQPGQRIRLGGLVEKGTIQRGQGATVAFSVTDTH
KSVKVTYTGILPDLFREEQGVITEGSFGPDGVFVADSVLAKHDERYMPKEVADGLKAKGVWQESKSE

Sequences:

>Translated_147_residues
MTRKQKRLSVIVGGLAFLGAATGLTFYALGQKASYFYMPADLTTASVQPGQRIRLGGLVEKGTIQRGQGATVAFSVTDTH
KSVKVTYTGILPDLFREEQGVITEGSFGPDGVFVADSVLAKHDERYMPKEVADGLKAKGVWQESKSE
>Mature_146_residues
TRKQKRLSVIVGGLAFLGAATGLTFYALGQKASYFYMPADLTTASVQPGQRIRLGGLVEKGTIQRGQGATVAFSVTDTHK
SVKVTYTGILPDLFREEQGVITEGSFGPDGVFVADSVLAKHDERYMPKEVADGLKAKGVWQESKSE

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family

Homologues:

Organism=Escherichia coli, GI1788525, Length=143, Percent_Identity=40.5594405594406, Blast_Score=107, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCME_RHILO (Q985G2)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107955.1
- ProteinModelPortal:   Q985G2
- SMR:   Q985G2
- GeneID:   1230610
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7689
- NMPDR:   fig|266835.1.peg.6056
- HOGENOM:   HBG296631
- OMA:   HVEFAVH
- ProtClustDB:   PRK13254
- HAMAP:   MF_01959
- InterPro:   IPR004329
- InterPro:   IPR012340
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF03100 CcmE; SSF82093 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 15830; Mature: 15699

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: BINDING 122-122

Signals:

None

Transmembrane regions:

HASH(0x177c6950)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKQKRLSVIVGGLAFLGAATGLTFYALGQKASYFYMPADLTTASVQPGQRIRLGGLVE
CCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCCEECCCCCCCEEEECCEEE
KGTIQRGQGATVAFSVTDTHKSVKVTYTGILPDLFREEQGVITEGSFGPDGVFVADSVLA
CCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHCCCCCEECCCCCCCCEEEHHHHHH
KHDERYMPKEVADGLKAKGVWQESKSE
HCCCCCCCHHHHCCCCCCCCCCCCCCH
>Mature Secondary Structure 
TRKQKRLSVIVGGLAFLGAATGLTFYALGQKASYFYMPADLTTASVQPGQRIRLGGLVE
CCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCCEECCCCCCCEEEECCEEE
KGTIQRGQGATVAFSVTDTHKSVKVTYTGILPDLFREEQGVITEGSFGPDGVFVADSVLA
CCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHCCCCCEECCCCCCCCEEEHHHHHH
KHDERYMPKEVADGLKAKGVWQESKSE
HCCCCCCCHHHHCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11214968