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The map label for this gene is potH [H]

Identifier: 13476376

GI number: 13476376

Start: 6356493

End: 6357326

Strand: Direct

Name: potH [H]

Synonym: mlr7677

Alternate gene names: 13476376

Gene position: 6356493-6357326 (Clockwise)

Preceding gene: 13476375

Following gene: 13476377

Centisome position: 90.34

GC content: 63.43

Gene sequence:

>834_bases
ATGAGAGGCGTTCTCTCCGACAGGATGGGCGCGGCGCTGTTGATGGCGCCGCTGCTCCTGTTCCTTGTGCTGGCCTATGC
CTGGCCGTTTCTCGGCGTGGTGAAGTGGAGTTTTACTCTGCCGACGCCGGGGCTTGGCCAATATGATGCCTTGCTTACCG
ACGATCTGGTCCAGTCGGTATTCATACGCACGCTGCGCATCGCGGCGATCGTCACGGTCGTGTCGGTCGCCGCCGCCTAT
GCCATCACGGTCGTCTGGGTGCGCGGCGGCCCGGCGCAGCGCCTCATCGCCGAATTCTGCATCCTGGTGCCGTTCTGGAT
CTCGGTGCTGACCCGCGCCTTCGGCTGGGTGGCGCTTTTGTCCAATCGCGGCCTGATCAACACCTGGCTGCAGTCGATCG
GTTTCATCTCCGAACCGCTGACCCTGGTGCGCAACGAGTTTGGCGTCGTCGTCGGCATGACGCATTTCCTCATCCCGTTC
GCGGTCTTTCCGCTGGCGTCCGCCATGCGCAGCCTAGACGACCGTGTGCTTCTGGCGGCGCGTGGGCTTGGCTCCAGCCG
CATGCGGACCTTCTGGACGGTGTTCGTACCGATGACACGCTCCGGCATCATCGGCTCGGCGCTGATCGTCTTCGTCTTCT
CACTCGGCTTCTTCGTCACGCCGGCGATCCTCGGTGGCGGCCGCAGCGTCATGATCGCCGAACTGATCTATCTCAGGATT
TTCCAGAGCCCGGACTGGGGGCTGGGAGCGGCGATTAGCGTCGTGCTGGTGCTGTTCGTCGGCGCGCTGATGGCGCTGCT
GTTCCGCTATGTCAGACCGAAGCAGTTGATCTGA

Upstream 100 bases:

>100_bases
CAGATCGCGCTCGACATGGATTGGTACGCCAAGAACTACGGCGCAGCGCTCGACGAATACACCAAGATCATCTCCGCCTG
ATCGGCAGCGATGATCTGAG

Downstream 100 bases:

>100_bases
TGCCGACCTATCGCCCCGGACCCGTCTCGCTGATCCTCGCCGCCCTTGTGGCGCTGTTCCTGCTGATGCCGCTGCTGGCG
GTCATCCCGGTGTCGCTGAC

Product: ABC transporter, permease protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MRGVLSDRMGAALLMAPLLLFLVLAYAWPFLGVVKWSFTLPTPGLGQYDALLTDDLVQSVFIRTLRIAAIVTVVSVAAAY
AITVVWVRGGPAQRLIAEFCILVPFWISVLTRAFGWVALLSNRGLINTWLQSIGFISEPLTLVRNEFGVVVGMTHFLIPF
AVFPLASAMRSLDDRVLLAARGLGSSRMRTFWTVFVPMTRSGIIGSALIVFVFSLGFFVTPAILGGGRSVMIAELIYLRI
FQSPDWGLGAAISVVLVLFVGALMALLFRYVRPKQLI

Sequences:

>Translated_277_residues
MRGVLSDRMGAALLMAPLLLFLVLAYAWPFLGVVKWSFTLPTPGLGQYDALLTDDLVQSVFIRTLRIAAIVTVVSVAAAY
AITVVWVRGGPAQRLIAEFCILVPFWISVLTRAFGWVALLSNRGLINTWLQSIGFISEPLTLVRNEFGVVVGMTHFLIPF
AVFPLASAMRSLDDRVLLAARGLGSSRMRTFWTVFVPMTRSGIIGSALIVFVFSLGFFVTPAILGGGRSVMIAELIYLRI
FQSPDWGLGAAISVVLVLFVGALMALLFRYVRPKQLI
>Mature_277_residues
MRGVLSDRMGAALLMAPLLLFLVLAYAWPFLGVVKWSFTLPTPGLGQYDALLTDDLVQSVFIRTLRIAAIVTVVSVAAAY
AITVVWVRGGPAQRLIAEFCILVPFWISVLTRAFGWVALLSNRGLINTWLQSIGFISEPLTLVRNEFGVVVGMTHFLIPF
AVFPLASAMRSLDDRVLLAARGLGSSRMRTFWTVFVPMTRSGIIGSALIVFVFSLGFFVTPAILGGGRSVMIAELIYLRI
FQSPDWGLGAAISVVLVLFVGALMALLFRYVRPKQLI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=237, Percent_Identity=31.2236286919831, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1787369, Length=239, Percent_Identity=31.7991631799163, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787713, Length=225, Percent_Identity=24, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30328; Mature: 30328

Theoretical pI: Translated: 11.46; Mature: 11.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]