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The map label for this gene is capD [H]

Identifier: 13476288

GI number: 13476288

Start: 6254075

End: 6256063

Strand: Direct

Name: capD [H]

Synonym: mlr7568

Alternate gene names: 13476288

Gene position: 6254075-6256063 (Clockwise)

Preceding gene: 13476286

Following gene: 13476289

Centisome position: 88.89

GC content: 61.24

Gene sequence:

>1989_bases
ATGACTTCCTATGCAGAAGCCGTGTTCGGATTTCGACCGAAAATGCGGCGCGTCATCATCATGATCCAGGATCTGCTCAT
GGTCCTGGTCGCGGTGGCGCTTAGTCTCGTCCTCTCCAGGTCAGACCTTTCGTTCGATGCATTCTCCTATGAAGGCCTGG
TCACCTGGGTCGGGATCGTTCTCGTCAGCCACTTGCTGTTCAGATATTGTGGTCTCTACACCACGATCTGGCGCTTTGCC
TCGACCCCGGACTTTTTCAACATTCTGAAAAGCTGTGCGATTCTGACGGTCGTGCTCTATACTCTTTCGCTGGTGTTTCG
TTTCTTCCAGCCCGTGGCTGGCCTCAACGAGCGCCAATTCATCGTCTTCTTCCTCGTTTCCTTCACCATCATATCGGCGC
CGAGGCTGTTCTACCGATTTCTTCGCGACGGCGCGAGCTGGGGCATCCTCACCCACAAGGCCAATAAAGTGCAGGCCAAG
CGGGCGCTGTTCGTCGGCCGGCTTGGCGAGGCCGACCTGATCATTCGCTTCACGCGCACGGCAGAGCCGGCCGACTATTC
CATTGCCGGCATCATGGCGACCGAGCGCGGCGCACCCGTGGGCACGCGGATTCAAGGTGTTGCCGTGGTGGCGTCCAGGC
CACGCCTGATCGATGTCCTGGAGGACTACGCGGCGGGCACCAAGAGCATCGACCTGCTTATTTTCGGCAGCGGCGTCGAG
CATGAGATCGAGGAGTATTCCGAGCTTGTGCGTGTCGCCCGGCACGGCGGCATAGCCGTCGCGCAGTTCTCGAGGCTTTC
GCAACTGGGGCAGGAGGGAAAGATCGTTCTCGACGAAGTCGAGATGGAAACGATCCTGCGCCGCCCGACCGTGCCGTCCG
ATATCGCGCGTATCGGTGCCTTCGTCGGCGGCAAGCGCGTCCTGGTGACGGGAGGCGCCGGATCTATCGGCCGGACCCTG
GTCAAGCGGTCGCTGGAGCTGGGGGCAGGGGCGGTGCTGGTCGCCGACAATTCCGAATTCGGCATCTTCCAGTTGAGCCA
GTATATCGACGAAAAAGACCATGATCGCCTGAAGGTCCGCATTGTCGATGTCGCGGACCGGCGCCAGATGACGCGAGTCG
TCACGGAATTCAAACCGGACATCATCTTCCATGCCGCGGCGCTGAAGCACGTTCCGCTGCTCGAGGAGAACTGGGAATCG
GCCATCCAGACCAATGTTTTCGGCACGCTTGTCTGCGCCGAGGTTGCCGCAAAATGTGGGGTTCCACAGTTCCTGCTGAT
ATCGAGCGACAAGGCCGTCGATCCGACCTCGGTGCTTGGCATCACAAAGAGGGCCGCCGAGCAGCTCGTCAGCGCCCTGC
ACGAAAGCCACGCAATCGCGCCGGACGGGCGCCGTTCCGGCACCAAGTTCATAGCCGTGCGGTTCGGCAATGTGTTTGGC
TCCAACGGCTCGGTGGCGACCATCTTCCAGGCACAGATCGAAGCCGGCGGTCCGGTCACGATTACCGATCGGCGCATGAC
CCGCTATTTCATGACGGTTGCGGAAGCCGTCGACCTCGTCATCATGGCGGCCGCCGACGCACAGTCGCGCAACGGCAAGG
ACGACTATGCCATCTACATGCTGGACATGGGCAAACCCGTGCCGATTCTCGAAGTCGCCGAAACCATGATCCGCATGGCC
GGCAAGACGCCCTATGCGGATATCCCGATCCGCTTCACCGGCATCAGGCCCGGTGAAAAGCTCCATGAGACGCTCCAGGG
CGCCAATGAGGAGATCGTCACGCTCGACATCGCCAAGATCTTTGGTCTCAGGACCGATGTGGTGGCGTGGCCGACGGTTC
AGGCAGCGCTGGCCGCGCTGCAGGTGGCCATGAAGAACCAGGACAAGGCTTCCGCCCTTGCGGTGTTGGCGCAACTCCCT
CGGCCGGAGACGCCAGCGCCCGACACGCTGCAGGAAACCGGCCCGAGAACGGTTGGGCAAGCAGGTTAG

Upstream 100 bases:

>100_bases
GAGGATTTGACGCTGAGAACAGGTGGTCGGTGACGGATGGTATTCGATCTGACGCCGCATTAGTGTTGAAGTTCTCACGC
CTGGAGTTCAATACTCGCCA

Downstream 100 bases:

>100_bases
ATACGTTGCAGCTGACGGTTGAATGGCGGGATTTTGCCCGGATATCGATCCCGCGGCCGGCGCCGTCGGAGCAATTCCCA
AGGGGAAAGCGCTACCGGCT

Product: lipopolysaccharide biosynthesis protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MTSYAEAVFGFRPKMRRVIIMIQDLLMVLVAVALSLVLSRSDLSFDAFSYEGLVTWVGIVLVSHLLFRYCGLYTTIWRFA
STPDFFNILKSCAILTVVLYTLSLVFRFFQPVAGLNERQFIVFFLVSFTIISAPRLFYRFLRDGASWGILTHKANKVQAK
RALFVGRLGEADLIIRFTRTAEPADYSIAGIMATERGAPVGTRIQGVAVVASRPRLIDVLEDYAAGTKSIDLLIFGSGVE
HEIEEYSELVRVARHGGIAVAQFSRLSQLGQEGKIVLDEVEMETILRRPTVPSDIARIGAFVGGKRVLVTGGAGSIGRTL
VKRSLELGAGAVLVADNSEFGIFQLSQYIDEKDHDRLKVRIVDVADRRQMTRVVTEFKPDIIFHAAALKHVPLLEENWES
AIQTNVFGTLVCAEVAAKCGVPQFLLISSDKAVDPTSVLGITKRAAEQLVSALHESHAIAPDGRRSGTKFIAVRFGNVFG
SNGSVATIFQAQIEAGGPVTITDRRMTRYFMTVAEAVDLVIMAAADAQSRNGKDDYAIYMLDMGKPVPILEVAETMIRMA
GKTPYADIPIRFTGIRPGEKLHETLQGANEEIVTLDIAKIFGLRTDVVAWPTVQAALAALQVAMKNQDKASALAVLAQLP
RPETPAPDTLQETGPRTVGQAG

Sequences:

>Translated_662_residues
MTSYAEAVFGFRPKMRRVIIMIQDLLMVLVAVALSLVLSRSDLSFDAFSYEGLVTWVGIVLVSHLLFRYCGLYTTIWRFA
STPDFFNILKSCAILTVVLYTLSLVFRFFQPVAGLNERQFIVFFLVSFTIISAPRLFYRFLRDGASWGILTHKANKVQAK
RALFVGRLGEADLIIRFTRTAEPADYSIAGIMATERGAPVGTRIQGVAVVASRPRLIDVLEDYAAGTKSIDLLIFGSGVE
HEIEEYSELVRVARHGGIAVAQFSRLSQLGQEGKIVLDEVEMETILRRPTVPSDIARIGAFVGGKRVLVTGGAGSIGRTL
VKRSLELGAGAVLVADNSEFGIFQLSQYIDEKDHDRLKVRIVDVADRRQMTRVVTEFKPDIIFHAAALKHVPLLEENWES
AIQTNVFGTLVCAEVAAKCGVPQFLLISSDKAVDPTSVLGITKRAAEQLVSALHESHAIAPDGRRSGTKFIAVRFGNVFG
SNGSVATIFQAQIEAGGPVTITDRRMTRYFMTVAEAVDLVIMAAADAQSRNGKDDYAIYMLDMGKPVPILEVAETMIRMA
GKTPYADIPIRFTGIRPGEKLHETLQGANEEIVTLDIAKIFGLRTDVVAWPTVQAALAALQVAMKNQDKASALAVLAQLP
RPETPAPDTLQETGPRTVGQAG
>Mature_661_residues
TSYAEAVFGFRPKMRRVIIMIQDLLMVLVAVALSLVLSRSDLSFDAFSYEGLVTWVGIVLVSHLLFRYCGLYTTIWRFAS
TPDFFNILKSCAILTVVLYTLSLVFRFFQPVAGLNERQFIVFFLVSFTIISAPRLFYRFLRDGASWGILTHKANKVQAKR
ALFVGRLGEADLIIRFTRTAEPADYSIAGIMATERGAPVGTRIQGVAVVASRPRLIDVLEDYAAGTKSIDLLIFGSGVEH
EIEEYSELVRVARHGGIAVAQFSRLSQLGQEGKIVLDEVEMETILRRPTVPSDIARIGAFVGGKRVLVTGGAGSIGRTLV
KRSLELGAGAVLVADNSEFGIFQLSQYIDEKDHDRLKVRIVDVADRRQMTRVVTEFKPDIIFHAAALKHVPLLEENWESA
IQTNVFGTLVCAEVAAKCGVPQFLLISSDKAVDPTSVLGITKRAAEQLVSALHESHAIAPDGRRSGTKFIAVRFGNVFGS
NGSVATIFQAQIEAGGPVTITDRRMTRYFMTVAEAVDLVIMAAADAQSRNGKDDYAIYMLDMGKPVPILEVAETMIRMAG
KTPYADIPIRFTGIRPGEKLHETLQGANEEIVTLDIAKIFGLRTDVVAWPTVQAALAALQVAMKNQDKASALAVLAQLPR
PETPAPDTLQETGPRTVGQAG

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI48994969, Length=112, Percent_Identity=33.9285714285714, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 72366; Mature: 72235

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYAEAVFGFRPKMRRVIIMIQDLLMVLVAVALSLVLSRSDLSFDAFSYEGLVTWVGIV
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LVSHLLFRYCGLYTTIWRFASTPDFFNILKSCAILTVVLYTLSLVFRFFQPVAGLNERQF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IVFFLVSFTIISAPRLFYRFLRDGASWGILTHKANKVQAKRALFVGRLGEADLIIRFTRT
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECC
AEPADYSIAGIMATERGAPVGTRIQGVAVVASRPRLIDVLEDYAAGTKSIDLLIFGSGVE
CCCCCCEEEEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCC
HEIEEYSELVRVARHGGIAVAQFSRLSQLGQEGKIVLDEVEMETILRRPTVPSDIARIGA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHH
FVGGKRVLVTGGAGSIGRTLVKRSLELGAGAVLVADNSEFGIFQLSQYIDEKDHDRLKVR
HCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEE
IVDVADRRQMTRVVTEFKPDIIFHAAALKHVPLLEENWESAIQTNVFGTLVCAEVAAKCG
EEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPQFLLISSDKAVDPTSVLGITKRAAEQLVSALHESHAIAPDGRRSGTKFIAVRFGNVFG
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCEEC
SNGSVATIFQAQIEAGGPVTITDRRMTRYFMTVAEAVDLVIMAAADAQSRNGKDDYAIYM
CCCCEEEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEE
LDMGKPVPILEVAETMIRMAGKTPYADIPIRFTGIRPGEKLHETLQGANEEIVTLDIAKI
EECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCEEEEEHHHH
FGLRTDVVAWPTVQAALAALQVAMKNQDKASALAVLAQLPRPETPAPDTLQETGPRTVGQ
HCCCCCCEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCC
AG
CC
>Mature Secondary Structure 
TSYAEAVFGFRPKMRRVIIMIQDLLMVLVAVALSLVLSRSDLSFDAFSYEGLVTWVGIV
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LVSHLLFRYCGLYTTIWRFASTPDFFNILKSCAILTVVLYTLSLVFRFFQPVAGLNERQF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IVFFLVSFTIISAPRLFYRFLRDGASWGILTHKANKVQAKRALFVGRLGEADLIIRFTRT
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECC
AEPADYSIAGIMATERGAPVGTRIQGVAVVASRPRLIDVLEDYAAGTKSIDLLIFGSGVE
CCCCCCEEEEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCC
HEIEEYSELVRVARHGGIAVAQFSRLSQLGQEGKIVLDEVEMETILRRPTVPSDIARIGA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHH
FVGGKRVLVTGGAGSIGRTLVKRSLELGAGAVLVADNSEFGIFQLSQYIDEKDHDRLKVR
HCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEE
IVDVADRRQMTRVVTEFKPDIIFHAAALKHVPLLEENWESAIQTNVFGTLVCAEVAAKCG
EEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPQFLLISSDKAVDPTSVLGITKRAAEQLVSALHESHAIAPDGRRSGTKFIAVRFGNVFG
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCEEC
SNGSVATIFQAQIEAGGPVTITDRRMTRYFMTVAEAVDLVIMAAADAQSRNGKDDYAIYM
CCCCEEEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEE
LDMGKPVPILEVAETMIRMAGKTPYADIPIRFTGIRPGEKLHETLQGANEEIVTLDIAKI
EECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCEEEEEHHHH
FGLRTDVVAWPTVQAALAALQVAMKNQDKASALAVLAQLPRPETPAPDTLQETGPRTVGQ
HCCCCCCEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCC
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]