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The map label for this gene is rfbB [H]

Identifier: 13476279

GI number: 13476279

Start: 6240408

End: 6241466

Strand: Direct

Name: rfbB [H]

Synonym: mlr7558

Alternate gene names: 13476279

Gene position: 6240408-6241466 (Clockwise)

Preceding gene: 13476278

Following gene: 13476280

Centisome position: 88.69

GC content: 57.13

Gene sequence:

>1059_bases
ATGTCTAAAACATGCGTGGTGACCGGCGGAGCTGGATTCATTGGATGCGCGTTGTCCACCAAATTGGCAAACCGCTTCGA
TCGCGTAGTTGTCATCGACAGTTTGCATCCGCAGATTCACGCAGAGCGAAAGCGTCCCGCCGACCTTGATCCTCGCGTTG
AACTCGTGGTTGCCGATGTGACCGAGGCCTCGACCTGGGTGCCGGCACTGGAAACCATCCGACCTGAAGTTGTGGTGCAT
CTGGCTGCCGAAACGGGCACGGGTCAGTCGCTCACGGAGGCCTCTCGCCACGCGATAGCAAATGTTCTTGGCACCACGCG
CATGTTGGATGCCTTTGCCACGGCATCGCACGTTCCTGAGCGTTTTGTCCTGGCTTCGAGTCGCGCCGTCTATGGAGAAG
GAGCATGGCGGTCCAGGAAAGCAGGCGAGATAACATATCCCGGTCAGAGATCGAAACAGCAGCTCGAACGCGCGGAGTGG
GATTTTCCCGGGCTCGAACCTCTACCGATGGACGCGGAAAAAACGATACCGAATCCAACCAGCATCTACGGAGCGACGAA
ACTAACCCAGGAACAGATATTGCGGGCTTGGGCTCTTTCGTTCGGAACAGCTGTCAACGTGCTTCGCCTTCAGAACGTAT
ATGGTCCTGGCCAATCGCTGACCAACAGCTACACGGGAATCGTGTCGTTGTTCATTAGGATGGCGAAGGAAGGCAAGAGC
ATCCCGATCTATGAGGACGGGGACATCGGACGCGACTTCGTCTTCATCGATGATGTGGCGTCGGCTCTCGACAAGGCTAC
GGCCACTGATCTGTCACAGTCGCTCGCCTATGATGTCGGCACTGGCTCCAAGACCACGGTTCTTGACCTGGCAAGGATCG
TTGCAAAAAGATACGAGGCCCCGGAACCGCACGTCAACGGCATGTTCCGCAACGGCGACGTTCGCTGCGCTGTCGCCAAC
ATCGACAGGACGGTGCGCGAACTGGAATGGCGACCTTTGAAAAGTGTAGACGAGGGGATCGGCCAGCTTTCCGAGTGGGT
CGATTCACAACTTGGCTAG

Upstream 100 bases:

>100_bases
TGAAATCCGATTCGATCGGAGCTGAACCTCCTGTGTCTATTCAAATGAGATCGAGGCTGATAACGAACGGCCAAGTCAAT
GAAACGGGAATGCTGCTTGA

Downstream 100 bases:

>100_bases
AATTATGACATCGTGACAACGGAAGCGTTCTGCCGTCGAGATGCCTCCATGGGAGACGTGGTCCCTATATCACGTGGATC
TGATTTTGCAGGGAATTGGC

Product: putative sugar nucleotide epimerase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSKTCVVTGGAGFIGCALSTKLANRFDRVVVIDSLHPQIHAERKRPADLDPRVELVVADVTEASTWVPALETIRPEVVVH
LAAETGTGQSLTEASRHAIANVLGTTRMLDAFATASHVPERFVLASSRAVYGEGAWRSRKAGEITYPGQRSKQQLERAEW
DFPGLEPLPMDAEKTIPNPTSIYGATKLTQEQILRAWALSFGTAVNVLRLQNVYGPGQSLTNSYTGIVSLFIRMAKEGKS
IPIYEDGDIGRDFVFIDDVASALDKATATDLSQSLAYDVGTGSKTTVLDLARIVAKRYEAPEPHVNGMFRNGDVRCAVAN
IDRTVRELEWRPLKSVDEGIGQLSEWVDSQLG

Sequences:

>Translated_352_residues
MSKTCVVTGGAGFIGCALSTKLANRFDRVVVIDSLHPQIHAERKRPADLDPRVELVVADVTEASTWVPALETIRPEVVVH
LAAETGTGQSLTEASRHAIANVLGTTRMLDAFATASHVPERFVLASSRAVYGEGAWRSRKAGEITYPGQRSKQQLERAEW
DFPGLEPLPMDAEKTIPNPTSIYGATKLTQEQILRAWALSFGTAVNVLRLQNVYGPGQSLTNSYTGIVSLFIRMAKEGKS
IPIYEDGDIGRDFVFIDDVASALDKATATDLSQSLAYDVGTGSKTTVLDLARIVAKRYEAPEPHVNGMFRNGDVRCAVAN
IDRTVRELEWRPLKSVDEGIGQLSEWVDSQLG
>Mature_351_residues
SKTCVVTGGAGFIGCALSTKLANRFDRVVVIDSLHPQIHAERKRPADLDPRVELVVADVTEASTWVPALETIRPEVVVHL
AAETGTGQSLTEASRHAIANVLGTTRMLDAFATASHVPERFVLASSRAVYGEGAWRSRKAGEITYPGQRSKQQLERAEWD
FPGLEPLPMDAEKTIPNPTSIYGATKLTQEQILRAWALSFGTAVNVLRLQNVYGPGQSLTNSYTGIVSLFIRMAKEGKSI
PIYEDGDIGRDFVFIDDVASALDKATATDLSQSLAYDVGTGSKTTVLDLARIVAKRYEAPEPHVNGMFRNGDVRCAVANI
DRTVRELEWRPLKSVDEGIGQLSEWVDSQLG

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=27.8551532033426, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI48994969, Length=365, Percent_Identity=25.7534246575342, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115532424, Length=352, Percent_Identity=25.8522727272727, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=298, Percent_Identity=24.8322147651007, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38367; Mature: 38236

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTCVVTGGAGFIGCALSTKLANRFDRVVVIDSLHPQIHAERKRPADLDPRVELVVADV
CCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCCCEEEEEEEC
TEASTWVPALETIRPEVVVHLAAETGTGQSLTEASRHAIANVLGTTRMLDAFATASHVPE
CCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RFVLASSRAVYGEGAWRSRKAGEITYPGQRSKQQLERAEWDFPGLEPLPMDAEKTIPNPT
HHEEECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCH
SIYGATKLTQEQILRAWALSFGTAVNVLRLQNVYGPGQSLTNSYTGIVSLFIRMAKEGKS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
IPIYEDGDIGRDFVFIDDVASALDKATATDLSQSLAYDVGTGSKTTVLDLARIVAKRYEA
CCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
PEPHVNGMFRNGDVRCAVANIDRTVRELEWRPLKSVDEGIGQLSEWVDSQLG
CCCCCCCEEECCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKTCVVTGGAGFIGCALSTKLANRFDRVVVIDSLHPQIHAERKRPADLDPRVELVVADV
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCCCEEEEEEEC
TEASTWVPALETIRPEVVVHLAAETGTGQSLTEASRHAIANVLGTTRMLDAFATASHVPE
CCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RFVLASSRAVYGEGAWRSRKAGEITYPGQRSKQQLERAEWDFPGLEPLPMDAEKTIPNPT
HHEEECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCH
SIYGATKLTQEQILRAWALSFGTAVNVLRLQNVYGPGQSLTNSYTGIVSLFIRMAKEGKS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
IPIYEDGDIGRDFVFIDDVASALDKATATDLSQSLAYDVGTGSKTTVLDLARIVAKRYEA
CCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
PEPHVNGMFRNGDVRCAVANIDRTVRELEWRPLKSVDEGIGQLSEWVDSQLG
CCCCCCCEEECCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]