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The map label for this gene is ispH

Identifier: 13476234

GI number: 13476234

Start: 6192527

End: 6193537

Strand: Direct

Name: ispH

Synonym: mlr7502

Alternate gene names: 13476234

Gene position: 6192527-6193537 (Clockwise)

Preceding gene: 13476233

Following gene: 13476235

Centisome position: 88.01

GC content: 65.18

Gene sequence:

>1011_bases
ATGCTTCAGACAACCAACAAGCCTCCGCTGACGATCCGGCTCTGCCAGCCGCGCGGTTTCTGCGCCGGCGTCGACCGTGC
CATCCAGATCGTCGTGCTGGCGCTGAAGAAGTACGGCGCGCCGGTCTATGTCCGCCACGAGATCGTGCACAACCGTTACG
TTGTCGAAGGGCTGCAGAGCCTCGGCGCCGTCTTCATCGAGGAACTCTCCGAGATTCCGGCTGAACACCGGCGGTCGCCG
GTTGTCTTCTCGGCGCACGGCGTGCCGAAATCAGTGCCGGCGGACGCGCAGGCGCGCAACCTGTTCTATCTTGACGCCAC
CTGTCCGCTGGTCTCCAAGGTGCACAAACAGGCAATGCGCCATCAGCGGCTTGGCCGCCATGTGCTGTTGATTGGCCATG
CCGGGCACCCCGAGGTGATCGGCACGATGGGGCAATTGCCGGAGGGCGCTGTAACGCTGGTCGAGACCGAGGCCGATGCC
GCCAGGCTCGTGCCCGCCGATCCGAAGGCGCTTGGCTTCGTCACCCAGACCACGCTGTCGGTCGAGGACACAGCCGGCAT
TATCCGTGCGCTGCGGGAGCGCTTTCCCGATCTGCAGGCGCCGGCGGCGGAATCGATCTGCTACGCCACCACCAACCGCC
AGGAGGCGGTCAAGGACACCGCCCCGGGCGCTGATCTCTATCTGATCGTCGGCGCCCCCAATTCCTCCAATTCGCGCCGC
CTCGTCGAAGTGGCGGAGCGTGCCGGCGCCACGATGTCGCTTCTCGTGCAGCGCGCCGCCGAAATTCCGTGGAATGACAT
CGGCACCATTTCGACGCTCGGCCTGTCGGCGGGGGCATCGGCGCCGGAGATCATCGTCGACGAGATCATCGACGCGTTCC
GCCAGCGCTTCGATGTCACCATCGACCTGGCCATCACGGCCACTGAAACAGAGGATTTTCCGGTCATGCGGGTACTGCGC
GATGTCGAACTGACGCCGGCCGACATGGCCTTCGTCAATGGGGCCGCGTAA

Upstream 100 bases:

>100_bases
AGCAGCGCCCCCCTTTCTACCGACACCCTTGACAGGGCCTGCTCTTGACAGAACGGGGGTAGGCACCTCATTTCGGCGCT
GACGAACCGGCCAGGATTTA

Downstream 100 bases:

>100_bases
GGCAGATGGCGGTCTATACCGACGTTGCGGAGGGCGAACTTGGCGCTTTCCTGAAACACTACCCCGTCGGCGATCTCCTG
TCCTACAAGGGCATCGCCGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MLQTTNKPPLTIRLCQPRGFCAGVDRAIQIVVLALKKYGAPVYVRHEIVHNRYVVEGLQSLGAVFIEELSEIPAEHRRSP
VVFSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVLLIGHAGHPEVIGTMGQLPEGAVTLVETEADA
ARLVPADPKALGFVTQTTLSVEDTAGIIRALRERFPDLQAPAAESICYATTNRQEAVKDTAPGADLYLIVGAPNSSNSRR
LVEVAERAGATMSLLVQRAAEIPWNDIGTISTLGLSAGASAPEIIVDEIIDAFRQRFDVTIDLAITATETEDFPVMRVLR
DVELTPADMAFVNGAA

Sequences:

>Translated_336_residues
MLQTTNKPPLTIRLCQPRGFCAGVDRAIQIVVLALKKYGAPVYVRHEIVHNRYVVEGLQSLGAVFIEELSEIPAEHRRSP
VVFSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVLLIGHAGHPEVIGTMGQLPEGAVTLVETEADA
ARLVPADPKALGFVTQTTLSVEDTAGIIRALRERFPDLQAPAAESICYATTNRQEAVKDTAPGADLYLIVGAPNSSNSRR
LVEVAERAGATMSLLVQRAAEIPWNDIGTISTLGLSAGASAPEIIVDEIIDAFRQRFDVTIDLAITATETEDFPVMRVLR
DVELTPADMAFVNGAA
>Mature_336_residues
MLQTTNKPPLTIRLCQPRGFCAGVDRAIQIVVLALKKYGAPVYVRHEIVHNRYVVEGLQSLGAVFIEELSEIPAEHRRSP
VVFSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVLLIGHAGHPEVIGTMGQLPEGAVTLVETEADA
ARLVPADPKALGFVTQTTLSVEDTAGIIRALRERFPDLQAPAAESICYATTNRQEAVKDTAPGADLYLIVGAPNSSNSRR
LVEVAERAGATMSLLVQRAAEIPWNDIGTISTLGLSAGASAPEIIVDEIIDAFRQRFDVTIDLAITATETEDFPVMRVLR
DVELTPADMAFVNGAA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=288, Percent_Identity=51.7361111111111, Blast_Score=295, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_RHILO (Q985W3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107804.1
- GeneID:   1230459
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7502
- NMPDR:   fig|266835.1.peg.5905
- HOGENOM:   HBG335228
- OMA:   WGAPVYV
- ProtClustDB:   PRK01045
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36290; Mature: 36290

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTTNKPPLTIRLCQPRGFCAGVDRAIQIVVLALKKYGAPVYVRHEIVHNRYVVEGLQS
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
LGAVFIEELSEIPAEHRRSPVVFSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMR
HHHHHHHHHHHCCHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
HQRLGRHVLLIGHAGHPEVIGTMGQLPEGAVTLVETEADAARLVPADPKALGFVTQTTLS
HHHCCCEEEEEECCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHEEEEEEEEC
VEDTAGIIRALRERFPDLQAPAAESICYATTNRQEAVKDTAPGADLYLIVGAPNSSNSRR
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCEEEEEECCCCCCCHH
LVEVAERAGATMSLLVQRAAEIPWNDIGTISTLGLSAGASAPEIIVDEIIDAFRQRFDVT
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHECCCCCCCCCCHHHHHHHHHHHHHHCCEE
IDLAITATETEDFPVMRVLRDVELTPADMAFVNGAA
EEEEEEECCCCCCHHHHHHHHCCCCCHHHHHCCCCC
>Mature Secondary Structure
MLQTTNKPPLTIRLCQPRGFCAGVDRAIQIVVLALKKYGAPVYVRHEIVHNRYVVEGLQS
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
LGAVFIEELSEIPAEHRRSPVVFSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMR
HHHHHHHHHHHCCHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
HQRLGRHVLLIGHAGHPEVIGTMGQLPEGAVTLVETEADAARLVPADPKALGFVTQTTLS
HHHCCCEEEEEECCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHEEEEEEEEC
VEDTAGIIRALRERFPDLQAPAAESICYATTNRQEAVKDTAPGADLYLIVGAPNSSNSRR
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCEEEEEECCCCCCCHH
LVEVAERAGATMSLLVQRAAEIPWNDIGTISTLGLSAGASAPEIIVDEIIDAFRQRFDVT
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHECCCCCCCCCCHHHHHHHHHHHHHHCCEE
IDLAITATETEDFPVMRVLRDVELTPADMAFVNGAA
EEEEEEECCCCCCHHHHHHHHCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968