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The map label for this gene is puuR [H]

Identifier: 13476145

GI number: 13476145

Start: 6108927

End: 6109610

Strand: Direct

Name: puuR [H]

Synonym: mlr7385

Alternate gene names: 13476145

Gene position: 6108927-6109610 (Clockwise)

Preceding gene: 13476144

Following gene: 13476146

Centisome position: 86.82

GC content: 65.35

Gene sequence:

>684_bases
TTGGCTTTTCGTTCGCTGCGCGAACCGTTTCTCACCCCACAAGGGGAGAAGGAAGAAGCAAACGAGGACCGACGCATGGA
CATGCTTGATGAGGCCGCCGAAAAACCCAAGGACGACGCCGATGTTCGCGTCGGCCGACGCGTGCGGGCGCTGCGGCTGG
AGCGCCGCCTGTCGCTGGCCGAACTGGCGGCAAAAGCCGGCGTGTCGATCGGTGCGCTGAGCCAGATCGAGCGCGGCATG
TCCTCGCTGCGCGTCAAGGTGATCTGGCCGCTCGCCGCCGCCCTCGACATCGAGCCGTCTGCGCTGATCGCCGACGGCAA
TGATGCCGTCAACGATCTCTATTGCGTGCGCGCCGACAAGCGGCGCTCCATTCCCGTCAAGTCGGAAGGCATCGCCAAGG
CGCTGCTGTCGCCGCCTGGCGCGACGCTGACCGGCATGCTGGTGGCGGTGGAAGCCGGCGGCGGCACGGCGGAAGCCTAC
GCCCATGCCGGCCATGAATTCGGCTATGTGCTGGCGGGCGAGGTCGAACTGGTGGTGGACTCGACAAAATACGGGCTGAA
GACCGGCGACAGTTTTGCCTTCAAGAGCACGCTGCTGCATGCCTTCCGCAATCCCGGCGCCGAGCAGTGCCAGATCCTCT
GGGTCAACACCACAAAACCCTCCGAGGTTCGCGATGGCGCCTGA

Upstream 100 bases:

>100_bases
CCGGGACGCTGCGATGCAGCGGGGACCCGGCAGGGTGGATCGGCTACCGCGCCCGGCCCTTCGCAGGCTCAGGGCGTTCG
AAACTCGAAAAGCCAAGCAA

Downstream 100 bases:

>100_bases
TCCACTGGTCCGGTTGCAGGCGGTCTCCAAGGTTTTTCCCGGCGGCATCGTCGGGCTCGATGCCGTCGATCTCGACATTG
TCAGCGGCGAGTTCATCACG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MAFRSLREPFLTPQGEKEEANEDRRMDMLDEAAEKPKDDADVRVGRRVRALRLERRLSLAELAAKAGVSIGALSQIERGM
SSLRVKVIWPLAAALDIEPSALIADGNDAVNDLYCVRADKRRSIPVKSEGIAKALLSPPGATLTGMLVAVEAGGGTAEAY
AHAGHEFGYVLAGEVELVVDSTKYGLKTGDSFAFKSTLLHAFRNPGAEQCQILWVNTTKPSEVRDGA

Sequences:

>Translated_227_residues
MAFRSLREPFLTPQGEKEEANEDRRMDMLDEAAEKPKDDADVRVGRRVRALRLERRLSLAELAAKAGVSIGALSQIERGM
SSLRVKVIWPLAAALDIEPSALIADGNDAVNDLYCVRADKRRSIPVKSEGIAKALLSPPGATLTGMLVAVEAGGGTAEAY
AHAGHEFGYVLAGEVELVVDSTKYGLKTGDSFAFKSTLLHAFRNPGAEQCQILWVNTTKPSEVRDGA
>Mature_226_residues
AFRSLREPFLTPQGEKEEANEDRRMDMLDEAAEKPKDDADVRVGRRVRALRLERRLSLAELAAKAGVSIGALSQIERGMS
SLRVKVIWPLAAALDIEPSALIADGNDAVNDLYCVRADKRRSIPVKSEGIAKALLSPPGATLTGMLVAVEAGGGTAEAYA
HAGHEFGYVLAGEVELVVDSTKYGLKTGDSFAFKSTLLHAFRNPGAEQCQILWVNTTKPSEVRDGA

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=181, Percent_Identity=25.9668508287293, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 24462; Mature: 24331

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFRSLREPFLTPQGEKEEANEDRRMDMLDEAAEKPKDDADVRVGRRVRALRLERRLSLA
CCCHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ELAAKAGVSIGALSQIERGMSSLRVKVIWPLAAALDIEPSALIADGNDAVNDLYCVRADK
HHHHHCCCCHHHHHHHHHHHHHCEEEEEEEEHHHCCCCCCEEEECCCCCCCCEEEEECCC
RRSIPVKSEGIAKALLSPPGATLTGMLVAVEAGGGTAEAYAHAGHEFGYVLAGEVELVVD
CCCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCEEEECEEEEEEE
STKYGLKTGDSFAFKSTLLHAFRNPGAEQCQILWVNTTKPSEVRDGA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AFRSLREPFLTPQGEKEEANEDRRMDMLDEAAEKPKDDADVRVGRRVRALRLERRLSLA
CCHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ELAAKAGVSIGALSQIERGMSSLRVKVIWPLAAALDIEPSALIADGNDAVNDLYCVRADK
HHHHHCCCCHHHHHHHHHHHHHCEEEEEEEEHHHCCCCCCEEEECCCCCCCCEEEEECCC
RRSIPVKSEGIAKALLSPPGATLTGMLVAVEAGGGTAEAYAHAGHEFGYVLAGEVELVVD
CCCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCEEEECEEEEEEE
STKYGLKTGDSFAFKSTLLHAFRNPGAEQCQILWVNTTKPSEVRDGA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]