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The map label for this gene is potD-A [H]

Identifier: 13476144

GI number: 13476144

Start: 6107669

End: 6108739

Strand: Direct

Name: potD-A [H]

Synonym: mlr7383

Alternate gene names: 13476144

Gene position: 6107669-6108739 (Clockwise)

Preceding gene: 13476143

Following gene: 13476145

Centisome position: 86.81

GC content: 61.25

Gene sequence:

>1071_bases
ATGACATTCCACCTGACATCGATAAGCGGACACAAGGCCCGCGCCGGCCTCGCCGTGCTGGCGCTCGCACTGTCTTCCAC
CGTGGCGCTTGCCGCCGAGAAGCTGCAATATTTCACCTGGTCGGGCTACGAACTGCCCGACTTCAACAAGAGCTTCCTCG
CCGCGCACCCCGACGGCGTCGAGGCCTCGATCTTCGGCGATGACGACGATGCCTTCACCAAGGTCAAGGCCGGCTTCCGG
CCCGACATCGCGCATCCCTGCTATGACAAGGTGGCGCGCTGGAACAAGGAAGGCCTGCTGCAGCCGATCGACACCAAGCG
CATCAAGAACTGGGATTCGATCTTCCCGGTGTTCAAGAACCTGCCCGATCTGCAGGCCGGCGACGGCAAGGTGTGGATGG
TGCCGTGGGATTGGGGCAACACTTCCATCCTCTACCGCACCGATCTGGTGAAGAACCCGGAAGCGAGCTGGAACCTGCTG
TGGGACAAGCAATATGCAGGCCGCATGGCGACCATCGACGCAGTGCACGACACGCCGATCGTTGCCGCGCTTCTGGCCGG
GGTGAACCCGTTCGATATGACCCCGGAGCAGATGGACAAGGTCGCGGCAAAACTGCGTGAACAGCGGCCGCTGCTGTCGA
GCTACACCACCGACATGACCTCGGTCGAACAGGCGCTTGCCAGCGGCCAGCTGGTCGCCGCCATGACCTGGAATGCGTCG
GCCACCTCGCTGAAGAAACAGGGCGTGCCGGTCGAGTTCATGAAGCCGAAAGAAGGCATGCTGACCTGGGCCTGCGGCTT
CGTCATGCTGAAAGACGCCAAGAATGTCGATCTCGCCTATGATTTCATCAACAGCCGGCTCGACGCCGATTCGGGCAAAT
TCCTGATCCAGTCCTACGGCTATGGCAGTTCGCTGAGCACTGCCTTTGCTGGCGTGGCGAAGGACGAGCTGGAGAAACTG
CAGCTGCCGTCCGATCCCGAGGTGATGCTGAAGAGCACCATCTTCACCGGGCCGATGAAGCAGAACGACGATGTGGCCAA
GATGTTCGAGAAAGTGAAGGCTGGCGGGTGA

Upstream 100 bases:

>100_bases
TCCTTCATCATCGCCGGCACCGCCGAAATCCTGCGCCATCGCGGGCTCGGCGCCGCGCGCCGCAATCCAGCCTGACCATA
ACAATGAGAGGAGAACGAAA

Downstream 100 bases:

>100_bases
GGCTCCCCTTCTCCCCTTGTGGGGTGAGCGGCCGGTTCGCGTAAGCGAATTCATCGTGCCAGTGGCACGATGAAAGGCCG
GCGAACGCCGGGACGCTGCG

Product: ABC transporter,binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTFHLTSISGHKARAGLAVLALALSSTVALAAEKLQYFTWSGYELPDFNKSFLAAHPDGVEASIFGDDDDAFTKVKAGFR
PDIAHPCYDKVARWNKEGLLQPIDTKRIKNWDSIFPVFKNLPDLQAGDGKVWMVPWDWGNTSILYRTDLVKNPEASWNLL
WDKQYAGRMATIDAVHDTPIVAALLAGVNPFDMTPEQMDKVAAKLREQRPLLSSYTTDMTSVEQALASGQLVAAMTWNAS
ATSLKKQGVPVEFMKPKEGMLTWACGFVMLKDAKNVDLAYDFINSRLDADSGKFLIQSYGYGSSLSTAFAGVAKDELEKL
QLPSDPEVMLKSTIFTGPMKQNDDVAKMFEKVKAGG

Sequences:

>Translated_356_residues
MTFHLTSISGHKARAGLAVLALALSSTVALAAEKLQYFTWSGYELPDFNKSFLAAHPDGVEASIFGDDDDAFTKVKAGFR
PDIAHPCYDKVARWNKEGLLQPIDTKRIKNWDSIFPVFKNLPDLQAGDGKVWMVPWDWGNTSILYRTDLVKNPEASWNLL
WDKQYAGRMATIDAVHDTPIVAALLAGVNPFDMTPEQMDKVAAKLREQRPLLSSYTTDMTSVEQALASGQLVAAMTWNAS
ATSLKKQGVPVEFMKPKEGMLTWACGFVMLKDAKNVDLAYDFINSRLDADSGKFLIQSYGYGSSLSTAFAGVAKDELEKL
QLPSDPEVMLKSTIFTGPMKQNDDVAKMFEKVKAGG
>Mature_355_residues
TFHLTSISGHKARAGLAVLALALSSTVALAAEKLQYFTWSGYELPDFNKSFLAAHPDGVEASIFGDDDDAFTKVKAGFRP
DIAHPCYDKVARWNKEGLLQPIDTKRIKNWDSIFPVFKNLPDLQAGDGKVWMVPWDWGNTSILYRTDLVKNPEASWNLLW
DKQYAGRMATIDAVHDTPIVAALLAGVNPFDMTPEQMDKVAAKLREQRPLLSSYTTDMTSVEQALASGQLVAAMTWNASA
TSLKKQGVPVEFMKPKEGMLTWACGFVMLKDAKNVDLAYDFINSRLDADSGKFLIQSYGYGSSLSTAFAGVAKDELEKLQ
LPSDPEVMLKSTIFTGPMKQNDDVAKMFEKVKAGG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=272, Percent_Identity=25, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787711, Length=202, Percent_Identity=29.2079207920792, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787078, Length=297, Percent_Identity=28.6195286195286, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39237; Mature: 39106

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFHLTSISGHKARAGLAVLALALSSTVALAAEKLQYFTWSGYELPDFNKSFLAAHPDGV
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHEEECCCCC
EASIFGDDDDAFTKVKAGFRPDIAHPCYDKVARWNKEGLLQPIDTKRIKNWDSIFPVFKN
EEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHC
LPDLQAGDGKVWMVPWDWGNTSILYRTDLVKNPEASWNLLWDKQYAGRMATIDAVHDTPI
CCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEECHHHCCCEEEEECCCCCHH
VAALLAGVNPFDMTPEQMDKVAAKLREQRPLLSSYTTDMTSVEQALASGQLVAAMTWNAS
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
ATSLKKQGVPVEFMKPKEGMLTWACGFVMLKDAKNVDLAYDFINSRLDADSGKFLIQSYG
HHHHHHCCCCEEEECCCCCCEEEEEHEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECC
YGSSLSTAFAGVAKDELEKLQLPSDPEVMLKSTIFTGPMKQNDDVAKMFEKVKAGG
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TFHLTSISGHKARAGLAVLALALSSTVALAAEKLQYFTWSGYELPDFNKSFLAAHPDGV
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHEEECCCCC
EASIFGDDDDAFTKVKAGFRPDIAHPCYDKVARWNKEGLLQPIDTKRIKNWDSIFPVFKN
EEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHC
LPDLQAGDGKVWMVPWDWGNTSILYRTDLVKNPEASWNLLWDKQYAGRMATIDAVHDTPI
CCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEECHHHCCCEEEEECCCCCHH
VAALLAGVNPFDMTPEQMDKVAAKLREQRPLLSSYTTDMTSVEQALASGQLVAAMTWNAS
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
ATSLKKQGVPVEFMKPKEGMLTWACGFVMLKDAKNVDLAYDFINSRLDADSGKFLIQSYG
HHHHHHCCCCEEEECCCCCCEEEEEHEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECC
YGSSLSTAFAGVAKDELEKLQLPSDPEVMLKSTIFTGPMKQNDDVAKMFEKVKAGG
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]