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The map label for this gene is puuA [H]

Identifier: 13476043

GI number: 13476043

Start: 5992543

End: 5993898

Strand: Reverse

Name: puuA [H]

Synonym: mll7254

Alternate gene names: 13476043

Gene position: 5993898-5992543 (Counterclockwise)

Preceding gene: 13476044

Following gene: 13476041

Centisome position: 85.19

GC content: 65.49

Gene sequence:

>1356_bases
TTGAGCGATGCAATTGCGGACGTTCTGAGCTGGCTTGAAAGCAGAAGCGACATACAGAGTCTGAGGGCGGCGGTGTGCGA
CCTCAACGGCGTCATGCGGGGAAAACGCATACCCGTGGAGCAGGCCCGCAAGGCGCTGGAAGGCAAGCTGCGCATGCCCT
ATTCGCTGATCGGGCTCGACATCTGGGGCGAGGACATCGAAGGCAACAGCCTGGTTTTCTCGACCGGCGATGCCGATGGG
CTCTGCCAATGGACGGGACGCGGCATCCTGCCGGTGGACTGGACGGCGCATCCGACGGCACTCGTTCCGCTCTGGCTCGC
CGACGAAAGCGGCGCGCCCTATCTCGGCGACCCCCGGCGGGCGCTGGCCCGCATCCTCGACCGCTACAAGGCGCTCGGCC
TGACCCCGGTCGCGGCGACGGAACTCGAATTCTATCTGGTCGATCCGCAATCGCAGCGGCCGGTCGGGCCGGTGTCACCG
GTCACCGGGCGGCGTCTCGATTCCGACGCGGCGCTGTCGATCGACGAGATCGACGATTTCGAGGCCTTCATCCACGACGT
CTATGAAGCCTGTCGGGCGCAAGGCATTCCCGTCGACACGGCGATTGCCGAAAACGGCGTCGGCCAGTTCGAGATCAATC
TGAACCATGTCGCCGACGCCCTGCGGGCGGCCGACGACGCGGTGCTGTTCAAGCGCACGGTGAAGGGCATTGCCCGCAAG
CACGGCTTTGCCGCCTGCTTCATGGCCAAACCCTATGGCGACCGGGCCGGCAACGGTTTTCACGTCCATTTCAGTCTGGT
CGACGCCGAAGGCCGCAACGTGTTCGACGATGGTACCGATCAGGGCTCGCAAACCATGCGCCACGCGGTCGGCGGGCTGC
TGGCGGCGATGGCCGAGAGCACGCTGGTGTTTGCGCCGCACTTCAATTCCTACCGCAGGCTTCGCCCGCGCTCCTATGCG
CCGACCGCGGTCGCCTGGGGCTACGAGAACCGCATGGTGGCGATCCGCATTCCCGGCGGTCCGGCCGGGGCGCGCCGGAT
CGAGCATCGCGTGTCGGGCGCTGACGCCAATCCCTATCTGGTGCTTGCCGCCATTTTGGGCGCCGCGCTGATCGGCATCG
AAAGGCAGATGTCGCCGGGCGAGCCGACCGGGAGCGACGGGCAAGGTGCAGTACCAGCCAAGCTGCCGCCGGACTGGGCA
TCCGCGATCGCCACCTTCGAAAGCGGCACGCATGTGGCGGAGATTTTCCCAGCGATCCTGCGCGACTCCTTCGTCGCCTG
CAAACGCCAGGAGTTGAACACCTTCGCGCTCAATGTCAGCGATTTCGAGATCGAGACCTATCTCGAAAGCGTCTGA

Upstream 100 bases:

>100_bases
GCAGCAGCCGGATAGGCTTTCCGAAAACTCACAAGAATTTTTATCAGGGATAGCGCAGCCGGCAGGGCTTTGGCACCTTC
GAAAACCAGAGGTTTTAAAA

Downstream 100 bases:

>100_bases
GACCTTACGAGGACCGCTCGGCCTTCAGCACCGAGACCGCCGCCTCGCTGTTGTGGACATAGTCGTTGGCGTCCGGCTGC
TTCTGCAGCAAAAGGATGAA

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSDAIADVLSWLESRSDIQSLRAAVCDLNGVMRGKRIPVEQARKALEGKLRMPYSLIGLDIWGEDIEGNSLVFSTGDADG
LCQWTGRGILPVDWTAHPTALVPLWLADESGAPYLGDPRRALARILDRYKALGLTPVAATELEFYLVDPQSQRPVGPVSP
VTGRRLDSDAALSIDEIDDFEAFIHDVYEACRAQGIPVDTAIAENGVGQFEINLNHVADALRAADDAVLFKRTVKGIARK
HGFAACFMAKPYGDRAGNGFHVHFSLVDAEGRNVFDDGTDQGSQTMRHAVGGLLAAMAESTLVFAPHFNSYRRLRPRSYA
PTAVAWGYENRMVAIRIPGGPAGARRIEHRVSGADANPYLVLAAILGAALIGIERQMSPGEPTGSDGQGAVPAKLPPDWA
SAIATFESGTHVAEIFPAILRDSFVACKRQELNTFALNVSDFEIETYLESV

Sequences:

>Translated_451_residues
MSDAIADVLSWLESRSDIQSLRAAVCDLNGVMRGKRIPVEQARKALEGKLRMPYSLIGLDIWGEDIEGNSLVFSTGDADG
LCQWTGRGILPVDWTAHPTALVPLWLADESGAPYLGDPRRALARILDRYKALGLTPVAATELEFYLVDPQSQRPVGPVSP
VTGRRLDSDAALSIDEIDDFEAFIHDVYEACRAQGIPVDTAIAENGVGQFEINLNHVADALRAADDAVLFKRTVKGIARK
HGFAACFMAKPYGDRAGNGFHVHFSLVDAEGRNVFDDGTDQGSQTMRHAVGGLLAAMAESTLVFAPHFNSYRRLRPRSYA
PTAVAWGYENRMVAIRIPGGPAGARRIEHRVSGADANPYLVLAAILGAALIGIERQMSPGEPTGSDGQGAVPAKLPPDWA
SAIATFESGTHVAEIFPAILRDSFVACKRQELNTFALNVSDFEIETYLESV
>Mature_450_residues
SDAIADVLSWLESRSDIQSLRAAVCDLNGVMRGKRIPVEQARKALEGKLRMPYSLIGLDIWGEDIEGNSLVFSTGDADGL
CQWTGRGILPVDWTAHPTALVPLWLADESGAPYLGDPRRALARILDRYKALGLTPVAATELEFYLVDPQSQRPVGPVSPV
TGRRLDSDAALSIDEIDDFEAFIHDVYEACRAQGIPVDTAIAENGVGQFEINLNHVADALRAADDAVLFKRTVKGIARKH
GFAACFMAKPYGDRAGNGFHVHFSLVDAEGRNVFDDGTDQGSQTMRHAVGGLLAAMAESTLVFAPHFNSYRRLRPRSYAP
TAVAWGYENRMVAIRIPGGPAGARRIEHRVSGADANPYLVLAAILGAALIGIERQMSPGEPTGSDGQGAVPAKLPPDWAS
AIATFESGTHVAEIFPAILRDSFVACKRQELNTFALNVSDFEIETYLESV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=429, Percent_Identity=24.9417249417249, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI87081870, Length=456, Percent_Identity=36.8421052631579, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1790301, Length=243, Percent_Identity=31.2757201646091, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 48755; Mature: 48623

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAIADVLSWLESRSDIQSLRAAVCDLNGVMRGKRIPVEQARKALEGKLRMPYSLIGLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEE
IWGEDIEGNSLVFSTGDADGLCQWTGRGILPVDWTAHPTALVPLWLADESGAPYLGDPRR
EECCCCCCCEEEEECCCCCCEEEECCCCCEECCCCCCCCEEEEEEEECCCCCCCCCCHHH
ALARILDRYKALGLTPVAATELEFYLVDPQSQRPVGPVSPVTGRRLDSDAALSIDEIDDF
HHHHHHHHHHHCCCCCEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHH
EAFIHDVYEACRAQGIPVDTAIAENGVGQFEINLNHVADALRAADDAVLFKRTVKGIARK
HHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGFAACFMAKPYGDRAGNGFHVHFSLVDAEGRNVFDDGTDQGSQTMRHAVGGLLAAMAES
CCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
TLVFAPHFNSYRRLRPRSYAPTAVAWGYENRMVAIRIPGGPAGARRIEHRVSGADANPYL
CEEECCCCCCHHHCCCCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHH
VLAAILGAALIGIERQMSPGEPTGSDGQGAVPAKLPPDWASAIATFESGTHVAEIFPAIL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
RDSFVACKRQELNTFALNVSDFEIETYLESV
HHHHHHHHHHCCCEEEEECCCHHHHHHHHCC
>Mature Secondary Structure 
SDAIADVLSWLESRSDIQSLRAAVCDLNGVMRGKRIPVEQARKALEGKLRMPYSLIGLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEE
IWGEDIEGNSLVFSTGDADGLCQWTGRGILPVDWTAHPTALVPLWLADESGAPYLGDPRR
EECCCCCCCEEEEECCCCCCEEEECCCCCEECCCCCCCCEEEEEEEECCCCCCCCCCHHH
ALARILDRYKALGLTPVAATELEFYLVDPQSQRPVGPVSPVTGRRLDSDAALSIDEIDDF
HHHHHHHHHHHCCCCCEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHH
EAFIHDVYEACRAQGIPVDTAIAENGVGQFEINLNHVADALRAADDAVLFKRTVKGIARK
HHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGFAACFMAKPYGDRAGNGFHVHFSLVDAEGRNVFDDGTDQGSQTMRHAVGGLLAAMAES
CCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
TLVFAPHFNSYRRLRPRSYAPTAVAWGYENRMVAIRIPGGPAGARRIEHRVSGADANPYL
CEEECCCCCCHHHCCCCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHH
VLAAILGAALIGIERQMSPGEPTGSDGQGAVPAKLPPDWASAIATFESGTHVAEIFPAIL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
RDSFVACKRQELNTFALNVSDFEIETYLESV
HHHHHHHHHHCCCEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]