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The map label for this gene is lip2 [H]

Identifier: 13476013

GI number: 13476013

Start: 5960155

End: 5961120

Strand: Reverse

Name: lip2 [H]

Synonym: mll7218

Alternate gene names: 13476013

Gene position: 5961120-5960155 (Counterclockwise)

Preceding gene: 13476014

Following gene: 13476011

Centisome position: 84.72

GC content: 67.08

Gene sequence:

>966_bases
ATGATGGAGATGGCGATGACGCTCGACCCTGATGCCTTGCGCGCCCTTGTAATCGGCCGGGTGGCCGGCGGTGAGCCGTT
CGAAAAGGGGAGCGTTGCGGCGGCGAGAGCCGCCTACAACGCCTCTTTCCCGACACAACAGGGCGAACATGAACCCGTCG
CCGCGAGCCAAGAACGCCAGACCGATGGGCCGAACGGCCCGGTCAAAATCCGCGTCCATCGCGGCATCGGCGCGCCGCGG
GCCGGGGCGCGCGCCGTGCTCTATCTGCATGGCGGCGGCTGGGTCATCGGCAATCTCTCCTCGCATGACGAGATCTGCCG
ATGGCTGGCCAATCTGAGCAACGCCGTCGTGGTCTACCCCGATTATCGACTGGCGCCGGAGCATAAATTTCCCGCCGGGC
TCGAGGATTGCACGGCAGCGCTCCGCTTCATGGCTGAGAATGCCGGCGAACTGGGCATCGACCCCGCCCGCATCAGCGTC
GCCGGCGACAGCGCCGGCGGCAACCTTGCCGCCGTCCTCGCATTGCTCGCCCGTGACAGGCTGGTACCCCGGCTTGCCGC
GCAGGTCCTGATCTATCCCAACACCGATGCCCGCCAGACGGCGGACAGCTATCGGCGTTTCGGCGACGGCTTCGGGCTCA
CCGCCGCCACCATGGCCTGGTTCCGCGACCACTATGTGCGGACGCCGAACGACATCACGGACTGGCGCGTCTCGCCGCTG
CTGGCATCAAGTCTTGCTGGCGCGGCACCTGCATTCGTCGTGATCGCCGGCCATGATATTCTTGCCGACGAAGGCACGGC
CTATGCCGAGCGTCTGCGGGTCGCAGGCGTGCCGCTGGTCCTGCGGCCCTGGCCGGGTCAAATCCACGGCTTCGTCTCGA
TGGGCCGCCATATACTGGCGGCGCGGCAAGCGGTGAGCGAGGCAGCGGCCTGGCTGCAATTGCAGACGCGTGTTCAGGCA
GACTGA

Upstream 100 bases:

>100_bases
CCGGCGAAACCAAAAGTGCCGTCGCCATTGCCGGCGATGTCGTCCAGCCGGCGCTGTTGACCGAGGACGAGGCAAACAGT
TTACTCGCCTAAGTGCGGCC

Downstream 100 bases:

>100_bases
CGCATCACCAGCGTCGCGCCGAGCTTGACGTTGCGCACCAGACCCTGGCCGGGTGGATCGGCCTCATCCAGCAAGGCCAG
CAGGATCTCGACGCTGCGGC

Product: lipase/esterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MMEMAMTLDPDALRALVIGRVAGGEPFEKGSVAAARAAYNASFPTQQGEHEPVAASQERQTDGPNGPVKIRVHRGIGAPR
AGARAVLYLHGGGWVIGNLSSHDEICRWLANLSNAVVVYPDYRLAPEHKFPAGLEDCTAALRFMAENAGELGIDPARISV
AGDSAGGNLAAVLALLARDRLVPRLAAQVLIYPNTDARQTADSYRRFGDGFGLTAATMAWFRDHYVRTPNDITDWRVSPL
LASSLAGAAPAFVVIAGHDILADEGTAYAERLRVAGVPLVLRPWPGQIHGFVSMGRHILAARQAVSEAAAWLQLQTRVQA
D

Sequences:

>Translated_321_residues
MMEMAMTLDPDALRALVIGRVAGGEPFEKGSVAAARAAYNASFPTQQGEHEPVAASQERQTDGPNGPVKIRVHRGIGAPR
AGARAVLYLHGGGWVIGNLSSHDEICRWLANLSNAVVVYPDYRLAPEHKFPAGLEDCTAALRFMAENAGELGIDPARISV
AGDSAGGNLAAVLALLARDRLVPRLAAQVLIYPNTDARQTADSYRRFGDGFGLTAATMAWFRDHYVRTPNDITDWRVSPL
LASSLAGAAPAFVVIAGHDILADEGTAYAERLRVAGVPLVLRPWPGQIHGFVSMGRHILAARQAVSEAAAWLQLQTRVQA
D
>Mature_321_residues
MMEMAMTLDPDALRALVIGRVAGGEPFEKGSVAAARAAYNASFPTQQGEHEPVAASQERQTDGPNGPVKIRVHRGIGAPR
AGARAVLYLHGGGWVIGNLSSHDEICRWLANLSNAVVVYPDYRLAPEHKFPAGLEDCTAALRFMAENAGELGIDPARISV
AGDSAGGNLAAVLALLARDRLVPRLAAQVLIYPNTDARQTADSYRRFGDGFGLTAATMAWFRDHYVRTPNDITDWRVSPL
LASSLAGAAPAFVVIAGHDILADEGTAYAERLRVAGVPLVLRPWPGQIHGFVSMGRHILAARQAVSEAAAWLQLQTRVQA
D

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=308, Percent_Identity=31.4935064935065, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI68299767, Length=332, Percent_Identity=28.3132530120482, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI157041239, Length=133, Percent_Identity=38.3458646616541, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI68051721, Length=153, Percent_Identity=37.2549019607843, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI61966717, Length=187, Percent_Identity=29.9465240641711, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI226423947, Length=161, Percent_Identity=35.4037267080745, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI157041237, Length=95, Percent_Identity=42.1052631578947, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI21328446, Length=100, Percent_Identity=40, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1786682, Length=259, Percent_Identity=32.046332046332, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=30.5660377358491, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI72001146, Length=154, Percent_Identity=39.6103896103896, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71996133, Length=145, Percent_Identity=40.6896551724138, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540028, Length=145, Percent_Identity=39.3103448275862, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34284; Mature: 34284

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEMAMTLDPDALRALVIGRVAGGEPFEKGSVAAARAAYNASFPTQQGEHEPVAASQERQ
CCCEEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TDGPNGPVKIRVHRGIGAPRAGARAVLYLHGGGWVIGNLSSHDEICRWLANLSNAVVVYP
CCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHHHCCCCCEEECC
DYRLAPEHKFPAGLEDCTAALRFMAENAGELGIDPARISVAGDSAGGNLAAVLALLARDR
CCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
LVPRLAAQVLIYPNTDARQTADSYRRFGDGFGLTAATMAWFRDHYVRTPNDITDWRVSPL
HHHHHHCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHH
LASSLAGAAPAFVVIAGHDILADEGTAYAERLRVAGVPLVLRPWPGQIHGFVSMGRHILA
HHHHHCCCCCEEEEEECCEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
ARQAVSEAAAWLQLQTRVQAD
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMEMAMTLDPDALRALVIGRVAGGEPFEKGSVAAARAAYNASFPTQQGEHEPVAASQERQ
CCCEEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TDGPNGPVKIRVHRGIGAPRAGARAVLYLHGGGWVIGNLSSHDEICRWLANLSNAVVVYP
CCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHHHCCCCCEEECC
DYRLAPEHKFPAGLEDCTAALRFMAENAGELGIDPARISVAGDSAGGNLAAVLALLARDR
CCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
LVPRLAAQVLIYPNTDARQTADSYRRFGDGFGLTAATMAWFRDHYVRTPNDITDWRVSPL
HHHHHHCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHH
LASSLAGAAPAFVVIAGHDILADEGTAYAERLRVAGVPLVLRPWPGQIHGFVSMGRHILA
HHHHHCCCCCEEEEEECCEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
ARQAVSEAAAWLQLQTRVQAD
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]