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The map label for this gene is afr [H]

Identifier: 13476014

GI number: 13476014

Start: 5961129

End: 5962277

Strand: Reverse

Name: afr [H]

Synonym: mll7219

Alternate gene names: 13476014

Gene position: 5962277-5961129 (Counterclockwise)

Preceding gene: 13476015

Following gene: 13476013

Centisome position: 84.74

GC content: 64.66

Gene sequence:

>1149_bases
ATGCAGGCAAAATCAGAGGCGAAGCTCGCAATTGGCGTCATCGGGTGTGGCAACATCTCAATGACCTACCTGCGCAATGC
AGCCCTCTTCGGTGGTATCGAACTGCGCGCCTGCGCCGATATCTCCGCCGACATGGCGGCGTTGCGGGCGAAGGAATATG
GCATCCAGGCGCTTGGCGTCGATGCGCTGCTTTCCGACCCCGAGATCGATCTCGTCCTCAACCTGACCATTCCGGCGGCG
CATTTCGATATTTCGTTGTCAGCCCTTTCGGCCGGAAAACACGTCTTCACCGAAAAGCCGCTGGCAACGTCGGCCGGCGA
CGGGCGACGGCTGGTGGCGGAGGCGGCCAAGCGCGGCGTGCTGCTCGGCTCGGCGCCCGACACGTTCCTCGGCGCCGCCG
GGCGCCGGGCGCGCCGCCTGATGGATGAGGGCGCCATCGGCCGCCCGGTCACCGGCACGGCCTTCATGATGGGCCGTGGC
ATGGAGCACTGGCACCCCAACCCGCAATTCTACTACCAGCCAGGCGGCGGCCCGGTGTTCGACATGGGTCCCTATTACCT
GACTATGCTGGTCAATCTGCTCGGCCCGGTCGCGCGCGTCATGGCGATGGCGACACGCGGCCAGGAGGAACGTCTGATCA
CTGCCGAAGGGCCGTTCAAGAACACGACATTCAAGGTCGGCACGCCGACCAATGTGCTGTCCCTGCTCGAATTCCGCTCT
GGCGCCACCGTTACTTTCGGCGCCTCATGGGACGTCTTCAAGCATTCCAACCATCCGATCGAATTGCATGGCACGGAGGG
CTCGCTGCGCCTGCCCGATCCCGACACGTTCGGCGGCACTGTCTCGCTGTCCGAACGCGGCGCGGACTGGAAGGATTTCG
CCGGCGAAGGCGAGCTCTACGGCGCCCGCAACTGGCCCTACGCCGCACCTGACCGCGCCAACTACCGCATGCTCGGCGTC
GCCGATCTGGTGCGCTCGCTGAAGACAGGTGCCGTGCCCCGCGCCTCCGGCAATCTGGCGCTGCATGTGCTGGAAATCAT
GGAGGCGATTCTGCATTCCGGCGAAACCAAAAGTGCCGTCGCCATTGCCGGCGATGTCGTCCAGCCGGCGCTGTTGACCG
AGGACGAGGCAAACAGTTTACTCGCCTAA

Upstream 100 bases:

>100_bases
CGTCAAGATTTAAAATGTTGTATAGCGCTCTTCCACCACGATCCGTTGCCGGCGCGGCGGATCAAGCAGAGCATGACCCT
ATTGGGAGGACCGATCAGAA

Downstream 100 bases:

>100_bases
GTGCGGCCATGATGGAGATGGCGATGACGCTCGACCCTGATGCCTTGCGCGCCCTTGTAATCGGCCGGGTGGCCGGCGGT
GAGCCGTTCGAAAAGGGGAG

Product: dehydrogenase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MQAKSEAKLAIGVIGCGNISMTYLRNAALFGGIELRACADISADMAALRAKEYGIQALGVDALLSDPEIDLVLNLTIPAA
HFDISLSALSAGKHVFTEKPLATSAGDGRRLVAEAAKRGVLLGSAPDTFLGAAGRRARRLMDEGAIGRPVTGTAFMMGRG
MEHWHPNPQFYYQPGGGPVFDMGPYYLTMLVNLLGPVARVMAMATRGQEERLITAEGPFKNTTFKVGTPTNVLSLLEFRS
GATVTFGASWDVFKHSNHPIELHGTEGSLRLPDPDTFGGTVSLSERGADWKDFAGEGELYGARNWPYAAPDRANYRMLGV
ADLVRSLKTGAVPRASGNLALHVLEIMEAILHSGETKSAVAIAGDVVQPALLTEDEANSLLA

Sequences:

>Translated_382_residues
MQAKSEAKLAIGVIGCGNISMTYLRNAALFGGIELRACADISADMAALRAKEYGIQALGVDALLSDPEIDLVLNLTIPAA
HFDISLSALSAGKHVFTEKPLATSAGDGRRLVAEAAKRGVLLGSAPDTFLGAAGRRARRLMDEGAIGRPVTGTAFMMGRG
MEHWHPNPQFYYQPGGGPVFDMGPYYLTMLVNLLGPVARVMAMATRGQEERLITAEGPFKNTTFKVGTPTNVLSLLEFRS
GATVTFGASWDVFKHSNHPIELHGTEGSLRLPDPDTFGGTVSLSERGADWKDFAGEGELYGARNWPYAAPDRANYRMLGV
ADLVRSLKTGAVPRASGNLALHVLEIMEAILHSGETKSAVAIAGDVVQPALLTEDEANSLLA
>Mature_382_residues
MQAKSEAKLAIGVIGCGNISMTYLRNAALFGGIELRACADISADMAALRAKEYGIQALGVDALLSDPEIDLVLNLTIPAA
HFDISLSALSAGKHVFTEKPLATSAGDGRRLVAEAAKRGVLLGSAPDTFLGAAGRRARRLMDEGAIGRPVTGTAFMMGRG
MEHWHPNPQFYYQPGGGPVFDMGPYYLTMLVNLLGPVARVMAMATRGQEERLITAEGPFKNTTFKVGTPTNVLSLLEFRS
GATVTFGASWDVFKHSNHPIELHGTEGSLRLPDPDTFGGTVSLSERGADWKDFAGEGELYGARNWPYAAPDRANYRMLGV
ADLVRSLKTGAVPRASGNLALHVLEIMEAILHSGETKSAVAIAGDVVQPALLTEDEANSLLA

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 40594; Mature: 40594

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAKSEAKLAIGVIGCGNISMTYLRNAALFGGIELRACADISADMAALRAKEYGIQALGV
CCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCEEECH
DALLSDPEIDLVLNLTIPAAHFDISLSALSAGKHVFTEKPLATSAGDGRRLVAEAAKRGV
HHHCCCCCEEEEEEEEECCEEEEEEEEHHHCCCEEECCCCCCCCCCCCHHHHHHHHHCCE
LLGSAPDTFLGAAGRRARRLMDEGAIGRPVTGTAFMMGRGMEHWHPNPQFYYQPGGGPVF
EECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHCCCHHHCCCCCCEEEECCCCCCC
DMGPYYLTMLVNLLGPVARVMAMATRGQEERLITAEGPFKNTTFKVGTPTNVLSLLEFRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHCC
GATVTFGASWDVFKHSNHPIELHGTEGSLRLPDPDTFGGTVSLSERGADWKDFAGEGELY
CCEEEECCCCCCEECCCCCEEEECCCCCEECCCCCCCCCEEEECCCCCCHHHCCCCCCEE
GARNWPYAAPDRANYRMLGVADLVRSLKTGAVPRASGNLALHVLEIMEAILHSGETKSAV
ECCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
AIAGDVVQPALLTEDEANSLLA
EEECCHHCCHHCCCCCHHCCCC
>Mature Secondary Structure
MQAKSEAKLAIGVIGCGNISMTYLRNAALFGGIELRACADISADMAALRAKEYGIQALGV
CCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCEEECH
DALLSDPEIDLVLNLTIPAAHFDISLSALSAGKHVFTEKPLATSAGDGRRLVAEAAKRGV
HHHCCCCCEEEEEEEEECCEEEEEEEEHHHCCCEEECCCCCCCCCCCCHHHHHHHHHCCE
LLGSAPDTFLGAAGRRARRLMDEGAIGRPVTGTAFMMGRGMEHWHPNPQFYYQPGGGPVF
EECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHCCCHHHCCCCCCEEEECCCCCCC
DMGPYYLTMLVNLLGPVARVMAMATRGQEERLITAEGPFKNTTFKVGTPTNVLSLLEFRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHCC
GATVTFGASWDVFKHSNHPIELHGTEGSLRLPDPDTFGGTVSLSERGADWKDFAGEGELY
CCEEEECCCCCCEECCCCCEEEECCCCCEECCCCCCCCCEEEECCCCCCHHHCCCCCCEE
GARNWPYAAPDRANYRMLGVADLVRSLKTGAVPRASGNLALHVLEIMEAILHSGETKSAV
ECCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
AIAGDVVQPALLTEDEANSLLA
EEECCHHCCHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA