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The map label for this gene is potB [H]

Identifier: 13475987

GI number: 13475987

Start: 5934351

End: 5935223

Strand: Reverse

Name: potB [H]

Synonym: mll7186

Alternate gene names: 13475987

Gene position: 5935223-5934351 (Counterclockwise)

Preceding gene: 13475988

Following gene: 13475986

Centisome position: 84.35

GC content: 62.54

Gene sequence:

>873_bases
ATGAGCGCGGGCGTCACGACACAGCCCGCGGAAAGGCGTTTGTCGCTGCTGGTGACCGTGCTGGCTTTCGCCCTGCCGGT
GATCTTCGTCCTGGTGCCGCTGGCGATCTTCCTGGTCTACAGTTTCTTCAGCGTCGACCAGGGCACGATCGTCTACACGC
CGACGCTCGGCAACTATGTCCGGTTCTTCACCGATCCGATCTTCCTGCCGGTGTTCTGGAACACCATCGTGCTGTGCGTG
TCGGTCGCCGTCATCTGCATCCTGCTCGCCTATCCCGCTGCCTATTTCCTGACGACGCTGATAGGGCGGTGGCGCTACGC
CTTGCTGATGCTGCTTCTGGTGCCGCTGCTGATGAGCTACGTCATCAAGATCTACGCGATCCGCAGCATCCTCGGCCTCA
ACGGCTTCCTCAACAAGGCGCTGGTGGCAATCGGCATCCTGCAGCAGCCGTCGACGCTTTTCGTGTTCAACATGAACGCC
ATCCTGTTGACGCTTAGCCTGCTTTTGATCCCCTTCGCGATCCTGCCGATCTTCCTGTCGCTGGAGCGGATACCGCAGAC
CTTGCTGCGTGCCTCCGACGATCTTGGCGCGAGCGGCTTGCAGACGTTCCTGCGCATCACCTTGCCGCTCAGCCTGCCCG
GCGTCGCGTCGGCGGCAAGCTTCGTCTTCGTGCTGGCGATCGGCGATTTCCTGACGCCGCAGATGGTCGGCGGAATCAGC
GGCTTCACCTTCGGCCGCATCCTCTATAGCCAGTTCGGCACGGCCTACAACTGGCCGTTCGGCGCCGCACTGTCGGTAGC
GCTGGCGATCGTGGTGATCTGCGCCATCCTCATCGGCGAACGGTTCGGGCGCAGCCGGGGGACGTCGGTATGA

Upstream 100 bases:

>100_bases
TGCGGCTGCGAACCCCAAGCCGGATCGACGGCGGCGCGCTGAGATCCGGCGAGACGGTTTCGCTTTCGTTTGCGCCGCAT
GAAGGCCATCTGGTGATGGC

Downstream 100 bases:

>100_bases
ACGACCTGCCCCGCCCCTCCACCATCTTCCTGGCCGCGATCACAACGCTGGTCGCGGTGCTGCTCTACAGCCCGCTCTTC
GTGCCGATCGTGTCGTCGTT

Product: ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSAGVTTQPAERRLSLLVTVLAFALPVIFVLVPLAIFLVYSFFSVDQGTIVYTPTLGNYVRFFTDPIFLPVFWNTIVLCV
SVAVICILLAYPAAYFLTTLIGRWRYALLMLLLVPLLMSYVIKIYAIRSILGLNGFLNKALVAIGILQQPSTLFVFNMNA
ILLTLSLLLIPFAILPIFLSLERIPQTLLRASDDLGASGLQTFLRITLPLSLPGVASAASFVFVLAIGDFLTPQMVGGIS
GFTFGRILYSQFGTAYNWPFGAALSVALAIVVICAILIGERFGRSRGTSV

Sequences:

>Translated_290_residues
MSAGVTTQPAERRLSLLVTVLAFALPVIFVLVPLAIFLVYSFFSVDQGTIVYTPTLGNYVRFFTDPIFLPVFWNTIVLCV
SVAVICILLAYPAAYFLTTLIGRWRYALLMLLLVPLLMSYVIKIYAIRSILGLNGFLNKALVAIGILQQPSTLFVFNMNA
ILLTLSLLLIPFAILPIFLSLERIPQTLLRASDDLGASGLQTFLRITLPLSLPGVASAASFVFVLAIGDFLTPQMVGGIS
GFTFGRILYSQFGTAYNWPFGAALSVALAIVVICAILIGERFGRSRGTSV
>Mature_289_residues
SAGVTTQPAERRLSLLVTVLAFALPVIFVLVPLAIFLVYSFFSVDQGTIVYTPTLGNYVRFFTDPIFLPVFWNTIVLCVS
VAVICILLAYPAAYFLTTLIGRWRYALLMLLLVPLLMSYVIKIYAIRSILGLNGFLNKALVAIGILQQPSTLFVFNMNAI
LLTLSLLLIPFAILPIFLSLERIPQTLLRASDDLGASGLQTFLRITLPLSLPGVASAASFVFVLAIGDFLTPQMVGGISG
FTFGRILYSQFGTAYNWPFGAALSVALAIVVICAILIGERFGRSRGTSV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=265, Percent_Identity=34.3396226415094, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787080, Length=244, Percent_Identity=30.327868852459, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787713, Length=257, Percent_Identity=32.6848249027237, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31559; Mature: 31428

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAGVTTQPAERRLSLLVTVLAFALPVIFVLVPLAIFLVYSFFSVDQGTIVYTPTLGNYV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHH
RFFTDPIFLPVFWNTIVLCVSVAVICILLAYPAAYFLTTLIGRWRYALLMLLLVPLLMSY
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIKIYAIRSILGLNGFLNKALVAIGILQQPSTLFVFNMNAILLTLSLLLIPFAILPIFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
LERIPQTLLRASDDLGASGLQTFLRITLPLSLPGVASAASFVFVLAIGDFLTPQMVGGIS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GFTFGRILYSQFGTAYNWPFGAALSVALAIVVICAILIGERFGRSRGTSV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]