Click here to switch to the map view.

The map label for this gene is mcbR [H]

Identifier: 13475972

GI number: 13475972

Start: 5922012

End: 5922668

Strand: Direct

Name: mcbR [H]

Synonym: mlr7171

Alternate gene names: 13475972

Gene position: 5922012-5922668 (Clockwise)

Preceding gene: 13475971

Following gene: 13475978

Centisome position: 84.17

GC content: 62.86

Gene sequence:

>657_bases
ATGCTGAAGCTCGAACATCAAACCCTCAACGATCGCGCCTATGGCGCGCTCAAGCAGGAATTGATTTCAGGCGGTTTCAA
CCCAGGCCAGACCCTGGTCATCCGCAAGCTTGCCGAAACCTTCGGCATCTCGACGACGCCGATCCGTGAGGCGCTGCAAA
GGCTGGTGGCAGAGCGGCTGCTCGAGATGCAGAACAATCGCTCGATCATTGTGCCGCTGCTGTCGGCGCCGGCCTTCGAG
GAACTGACCCACATCCGCATCGCCGTCGAGGGGTTGGCGGGTGAGATGGCCGCCACGCGGATAAACGAAAGCGGGCTGGT
CGAGATACAGGCGACGCTCGCCGGCATGCAGCGCGCCATCGAGGCCGGCGACGCCAGGGCCTATCATGCGCTGAACGAGG
CCTTCCACTTCGCAATCTACCAGCACGCCGGTGCGCCGATCCTGCTCAACATGATCCGCGATCTCTGGGGCCGGGTCGGG
CCCTATCTCAAGCTGCTGATGCAGGCCGACCGCTATATTCCGCAGTCGAACGACGCGCATCGCAGGATTGTCGCTGCGTT
GGAGCAGCGCGATGGCTCTGCTGTCCGGGTCTCCCTCGAGCAGGACATCGCCGTGGCGGCCGCGGTTCTTTCAGAAAATC
TTCGCACGACGACCTGA

Upstream 100 bases:

>100_bases
TGTGTTGAAGATCAGGCCGCCTCTGCCCTTTGCCAGGGACAATGCCGAGCAACTGGCCGAGACGCTCGATCGTGCTTTGT
CGAACTGGTGATCGGGACGC

Downstream 100 bases:

>100_bases
TCGACGGCAGGCTGGGTCGGGGCCTATGCCGTCTCGTCCAACCGGTCGCGCAGCATGCTCCAGCCAAGCAGCATGTTGGG
CGCGTGCCGCAACAAGGCAT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MLKLEHQTLNDRAYGALKQELISGGFNPGQTLVIRKLAETFGISTTPIREALQRLVAERLLEMQNNRSIIVPLLSAPAFE
ELTHIRIAVEGLAGEMAATRINESGLVEIQATLAGMQRAIEAGDARAYHALNEAFHFAIYQHAGAPILLNMIRDLWGRVG
PYLKLLMQADRYIPQSNDAHRRIVAALEQRDGSAVRVSLEQDIAVAAAVLSENLRTTT

Sequences:

>Translated_218_residues
MLKLEHQTLNDRAYGALKQELISGGFNPGQTLVIRKLAETFGISTTPIREALQRLVAERLLEMQNNRSIIVPLLSAPAFE
ELTHIRIAVEGLAGEMAATRINESGLVEIQATLAGMQRAIEAGDARAYHALNEAFHFAIYQHAGAPILLNMIRDLWGRVG
PYLKLLMQADRYIPQSNDAHRRIVAALEQRDGSAVRVSLEQDIAVAAAVLSENLRTTT
>Mature_218_residues
MLKLEHQTLNDRAYGALKQELISGGFNPGQTLVIRKLAETFGISTTPIREALQRLVAERLLEMQNNRSIIVPLLSAPAFE
ELTHIRIAVEGLAGEMAATRINESGLVEIQATLAGMQRAIEAGDARAYHALNEAFHFAIYQHAGAPILLNMIRDLWGRVG
PYLKLLMQADRYIPQSNDAHRRIVAALEQRDGSAVRVSLEQDIAVAAAVLSENLRTTT

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=209, Percent_Identity=33.0143540669856, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787821, Length=206, Percent_Identity=25.7281553398058, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082149, Length=187, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24016; Mature: 24016

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLEHQTLNDRAYGALKQELISGGFNPGQTLVIRKLAETFGISTTPIREALQRLVAERL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEMQNNRSIIVPLLSAPAFEELTHIRIAVEGLAGEMAATRINESGLVEIQATLAGMQRAI
HHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHH
EAGDARAYHALNEAFHFAIYQHAGAPILLNMIRDLWGRVGPYLKLLMQADRYIPQSNDAH
HHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
RRIVAALEQRDGSAVRVSLEQDIAVAAAVLSENLRTTT
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKLEHQTLNDRAYGALKQELISGGFNPGQTLVIRKLAETFGISTTPIREALQRLVAERL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEMQNNRSIIVPLLSAPAFEELTHIRIAVEGLAGEMAATRINESGLVEIQATLAGMQRAI
HHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHH
EAGDARAYHALNEAFHFAIYQHAGAPILLNMIRDLWGRVGPYLKLLMQADRYIPQSNDAH
HHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
RRIVAALEQRDGSAVRVSLEQDIAVAAAVLSENLRTTT
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]