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The map label for this gene is mcbR [H]

Identifier: 13475978

GI number: 13475978

Start: 5927489

End: 5928283

Strand: Direct

Name: mcbR [H]

Synonym: mlr7177

Alternate gene names: 13475978

Gene position: 5927489-5928283 (Clockwise)

Preceding gene: 13475972

Following gene: 13475979

Centisome position: 84.24

GC content: 62.77

Gene sequence:

>795_bases
TTGATCACGCTATTCGGCTTTAATTTCATCCGCAAGCCGAAAACGGGGCAAAAGCCGGGAATGCAGAAGGCTGCCATCGA
GCGAACCAACGAAACAATCGCAGCCCAGCTTACGCCGGTCGGCCGCGAGACCGTGCAGGATCGCGTCTATTCCGAATTGC
GCCGCGCCTTGATCGGCGGCCTGTTCGAGCCGAACCAGGTGCTGACCATCCGCGGCCTTGCCGATGCGCTGGTCACCTCG
ACCATGCCCGTGCGTGAAGCGCTCGGCCGGCTGATCACGGAAAAGGCGCTGGAGGCGCTGCCCAACCGCTCGGTGCGCGT
GCCGCCGATCACGCTGGAACGTATCGACGATCTCTTGCGTGCCCGCTCACTCATCGAAGGCGAAGCGATCGCGCTTGCCG
CCACGCGCATGAACCCGCGCCAGATCGCTTCGATTGAAGCTATGCTTGGCGAGTGGGATGAGATGCGGGCGCTGAAGCAC
AAGAAGGATATCGACCGCGAAGCGACGCTCAACCAGAGCTTCCATTTCGAGATCTACCGCTGCTGCGGTTCGGCCGTGCT
GATCCCGATGATCGAAAGCCTGTGGCTGCAATCGGGTCCCTGCATCCGCGTCGCCATCTATGCCTTTTCCGATGCCGGCG
AGGTCGACACCGCGCACTATCACCGTCGCATCGTCGCGGCGCTGGCCAAGCAGGATGCGCAAGCGGCGCGCGAGGCGCTG
GTGGCCGATATCAGCCGGCCTTTTGCCTTCCTGCGCGACAAGCTTCAATCCGCCGCCGCGAAAGACCACACATGA

Upstream 100 bases:

>100_bases
GGTCTCCTTCCCCTGCCGTTCGGTTGCCTCGCTCATCAAACCCTCTGGCCGAAGACGATCATCGCCGTCTCCTGATTTTT
GATTTGTGATCACACTTGCT

Downstream 100 bases:

>100_bases
ACAAGCCGGCTATCAGGATCACCGAACCGAGCTCGAAGCTTTCCGTGACGGCGCTGCTGCTGCCCGAAAAGGCGCCGGAA
AACGTGGCTTTCCTTTTGGC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MITLFGFNFIRKPKTGQKPGMQKAAIERTNETIAAQLTPVGRETVQDRVYSELRRALIGGLFEPNQVLTIRGLADALVTS
TMPVREALGRLITEKALEALPNRSVRVPPITLERIDDLLRARSLIEGEAIALAATRMNPRQIASIEAMLGEWDEMRALKH
KKDIDREATLNQSFHFEIYRCCGSAVLIPMIESLWLQSGPCIRVAIYAFSDAGEVDTAHYHRRIVAALAKQDAQAAREAL
VADISRPFAFLRDKLQSAAAKDHT

Sequences:

>Translated_264_residues
MITLFGFNFIRKPKTGQKPGMQKAAIERTNETIAAQLTPVGRETVQDRVYSELRRALIGGLFEPNQVLTIRGLADALVTS
TMPVREALGRLITEKALEALPNRSVRVPPITLERIDDLLRARSLIEGEAIALAATRMNPRQIASIEAMLGEWDEMRALKH
KKDIDREATLNQSFHFEIYRCCGSAVLIPMIESLWLQSGPCIRVAIYAFSDAGEVDTAHYHRRIVAALAKQDAQAAREAL
VADISRPFAFLRDKLQSAAAKDHT
>Mature_264_residues
MITLFGFNFIRKPKTGQKPGMQKAAIERTNETIAAQLTPVGRETVQDRVYSELRRALIGGLFEPNQVLTIRGLADALVTS
TMPVREALGRLITEKALEALPNRSVRVPPITLERIDDLLRARSLIEGEAIALAATRMNPRQIASIEAMLGEWDEMRALKH
KKDIDREATLNQSFHFEIYRCCGSAVLIPMIESLWLQSGPCIRVAIYAFSDAGEVDTAHYHRRIVAALAKQDAQAAREAL
VADISRPFAFLRDKLQSAAAKDHT

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=207, Percent_Identity=28.0193236714976, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 29387; Mature: 29387

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLFGFNFIRKPKTGQKPGMQKAAIERTNETIAAQLTPVGRETVQDRVYSELRRALIGG
CEEEEECHHHCCCCCCCCCCHHHHHHHHHCCHHEEHCCCCCHHHHHHHHHHHHHHHHHCC
LFEPNQVLTIRGLADALVTSTMPVREALGRLITEKALEALPNRSVRVPPITLERIDDLLR
CCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHH
ARSLIEGEAIALAATRMNPRQIASIEAMLGEWDEMRALKHKKDIDREATLNQSFHFEIYR
HHHHHCCCHHEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHH
CCGSAVLIPMIESLWLQSGPCIRVAIYAFSDAGEVDTAHYHRRIVAALAKQDAQAAREAL
HHCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VADISRPFAFLRDKLQSAAAKDHT
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MITLFGFNFIRKPKTGQKPGMQKAAIERTNETIAAQLTPVGRETVQDRVYSELRRALIGG
CEEEEECHHHCCCCCCCCCCHHHHHHHHHCCHHEEHCCCCCHHHHHHHHHHHHHHHHHCC
LFEPNQVLTIRGLADALVTSTMPVREALGRLITEKALEALPNRSVRVPPITLERIDDLLR
CCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHH
ARSLIEGEAIALAATRMNPRQIASIEAMLGEWDEMRALKHKKDIDREATLNQSFHFEIYR
HHHHHCCCHHEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHH
CCGSAVLIPMIESLWLQSGPCIRVAIYAFSDAGEVDTAHYHRRIVAALAKQDAQAAREAL
HHCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VADISRPFAFLRDKLQSAAAKDHT
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]