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The map label for this gene is budB [H]

Identifier: 13475967

GI number: 13475967

Start: 5915827

End: 5917473

Strand: Direct

Name: budB [H]

Synonym: mlr7164

Alternate gene names: 13475967

Gene position: 5915827-5917473 (Clockwise)

Preceding gene: 13475966

Following gene: 13475969

Centisome position: 84.08

GC content: 64.42

Gene sequence:

>1647_bases
ATGACCAAAGGTTCGGATCTGTTCGTCGCGGCCCTCGAGAATGAAGGGGTCGACCGGATCTTCGGCATTCCGGGCGAGGA
GAATCTCGACATCGTCGAATCCATCCGTCGCTCGTCGATCCAGTTGATCTTGACCCGCCACGAGCAGGCGGCCGCCTTCA
TGGCCGCCACCTATGGCAGGCTCACCGGCAAGGCAGGCGTGTGCATCACCACGCTTGGCCCCGGCGCGCTCAATCTGACG
ACCGGCGCGGCCTATGCGCTGCTCGGCGCGATGCCGATGATCATGATCACCGGTCAGAAGGGCATATTGTCATCGCGGCA
GGCGCGTTTCCAGATCGTCGACATCGTCGCGGCGATGAAGCCGCTGACCAAGCTGTCGCGCCAGATCGTCTCGCCGAAGA
TGATCCCCTCGCTGGTGCGCGAAGCCTTCCGCGTTGCCCAGGAAGAGCGGCCCGGCCCCGTCCATCTGGAACTGCCGGAG
GACATTGCGGCGGCCGAATGCGAGCCGGTGGCGCTGGTGCCGACACACCCGGTCGAACTGCCGTTGGCCAGCCCGGGCGC
GCTCGATCGCGCCGCCCGCATGATCATGGAGGCCAAACATCCGCTGCTGATGTTCGGCGCGGCGGCGTCGCGGCCGCGCG
TGACCCCCGATGTCGCCCAGTTCGTGCTGCGCACGCAAATACCCTATTTCACCACGCAGATGGGCAAGGGCACGGTGCCT
GGCGGCACCGAACTCTACATGGGAACAGCGGCGCTGTCGGAGCGCGACTATGTGCACGAGGCCATCGAACAGGCCGATCT
GATCATAACGATCGGCCACGACACGGTGGAGAAACCGCCCTTCATCATGGGCGCCAATGGGCCGAAGGTCATCCATGTCG
GCTACCACTCGGCCGATGTCGAGCAGGTCTATTTCCCGCAGGCCGAGATCGTCGGCGATCTCGGCCCGTCGCTGGCGCTG
CTGGCGGACCGTATCGAAGGCAAGATCCCCAACGCGCAGGCCTTGCTGCCGCTCAGGGAAAACATTCTGAGCCGGATCGC
CGCGCGCGCCACCGAAGACCGCTTTACGCCGCAGCGCATCGTGCATGACGTGCGCGCTGTGATGCCGGCGGACGGGATCC
TTGCCCTCGACAACGGCATGTACAAGATCTGGTTCGCGCGCAACTACCGCACGCGCATGGCAAACACGCTGCTACTCGAC
AATGCGCTGGCCACGATGGGCGCCGGCCTGCCGTCGGCGATGATGGCGTCGCTGCTCTTTCCCACGCGCCGCGTGATGGC
CATTTGCGGCGACGGTGGGTTCATGATGAACAGCCAGGAACTGGAGACCGCCGTCAGGCTGAAGCTCAACCTCGTCATCC
TGCTGCTGGAAGACCATGCCTATGGCATGATCCGCTGGAAGCAGGCGGTCGACGAATTCCCGGATTTCGGCATGACCTTC
GGCAATCCGGATTTCGTCAAATACGCCGAGGCTTATGGCGCCAAGGGAACCAGGGTTACGGAGATCGCCGATTTGCGGCC
GGCGCTGGAACGGGCCTTCACCGGCGGCGGCGTACATCTCGTCGTCGTGCCCATCGACTATTCCGAAAACACCAGGGTGC
TTGTCGACGAATTACGCGAGCGGCTGCCGGCGCCGCGTACGCCCTGA

Upstream 100 bases:

>100_bases
GCTGATCGCAAACAGAGCAGGCTTGCGCTCTTTGCGGCGTCGCCTTCCCTGCCATATTAAGGTGGCTGACTGAATGTGCG
CATTGCAGGAGAGGCGGTTC

Downstream 100 bases:

>100_bases
CGGCAGCGGCAAAGCCACTGTCAGGACCTCTTGGTTTCATCTGGCGCCGGGCACCCATTCCGTTTGGATCAACGGGATTT
GCGGGGCGGCGTTGCGCGGC

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTKGSDLFVAALENEGVDRIFGIPGEENLDIVESIRRSSIQLILTRHEQAAAFMAATYGRLTGKAGVCITTLGPGALNLT
TGAAYALLGAMPMIMITGQKGILSSRQARFQIVDIVAAMKPLTKLSRQIVSPKMIPSLVREAFRVAQEERPGPVHLELPE
DIAAAECEPVALVPTHPVELPLASPGALDRAARMIMEAKHPLLMFGAAASRPRVTPDVAQFVLRTQIPYFTTQMGKGTVP
GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTVEKPPFIMGANGPKVIHVGYHSADVEQVYFPQAEIVGDLGPSLAL
LADRIEGKIPNAQALLPLRENILSRIAARATEDRFTPQRIVHDVRAVMPADGILALDNGMYKIWFARNYRTRMANTLLLD
NALATMGAGLPSAMMASLLFPTRRVMAICGDGGFMMNSQELETAVRLKLNLVILLLEDHAYGMIRWKQAVDEFPDFGMTF
GNPDFVKYAEAYGAKGTRVTEIADLRPALERAFTGGGVHLVVVPIDYSENTRVLVDELRERLPAPRTP

Sequences:

>Translated_548_residues
MTKGSDLFVAALENEGVDRIFGIPGEENLDIVESIRRSSIQLILTRHEQAAAFMAATYGRLTGKAGVCITTLGPGALNLT
TGAAYALLGAMPMIMITGQKGILSSRQARFQIVDIVAAMKPLTKLSRQIVSPKMIPSLVREAFRVAQEERPGPVHLELPE
DIAAAECEPVALVPTHPVELPLASPGALDRAARMIMEAKHPLLMFGAAASRPRVTPDVAQFVLRTQIPYFTTQMGKGTVP
GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTVEKPPFIMGANGPKVIHVGYHSADVEQVYFPQAEIVGDLGPSLAL
LADRIEGKIPNAQALLPLRENILSRIAARATEDRFTPQRIVHDVRAVMPADGILALDNGMYKIWFARNYRTRMANTLLLD
NALATMGAGLPSAMMASLLFPTRRVMAICGDGGFMMNSQELETAVRLKLNLVILLLEDHAYGMIRWKQAVDEFPDFGMTF
GNPDFVKYAEAYGAKGTRVTEIADLRPALERAFTGGGVHLVVVPIDYSENTRVLVDELRERLPAPRTP
>Mature_547_residues
TKGSDLFVAALENEGVDRIFGIPGEENLDIVESIRRSSIQLILTRHEQAAAFMAATYGRLTGKAGVCITTLGPGALNLTT
GAAYALLGAMPMIMITGQKGILSSRQARFQIVDIVAAMKPLTKLSRQIVSPKMIPSLVREAFRVAQEERPGPVHLELPED
IAAAECEPVALVPTHPVELPLASPGALDRAARMIMEAKHPLLMFGAAASRPRVTPDVAQFVLRTQIPYFTTQMGKGTVPG
GTELYMGTAALSERDYVHEAIEQADLIITIGHDTVEKPPFIMGANGPKVIHVGYHSADVEQVYFPQAEIVGDLGPSLALL
ADRIEGKIPNAQALLPLRENILSRIAARATEDRFTPQRIVHDVRAVMPADGILALDNGMYKIWFARNYRTRMANTLLLDN
ALATMGAGLPSAMMASLLFPTRRVMAICGDGGFMMNSQELETAVRLKLNLVILLLEDHAYGMIRWKQAVDEFPDFGMTFG
NPDFVKYAEAYGAKGTRVTEIADLRPALERAFTGGGVHLVVVPIDYSENTRVLVDELRERLPAPRTP

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=543, Percent_Identity=24.8618784530387, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=24.777183600713, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI1790104, Length=546, Percent_Identity=30.4029304029304, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI87081685, Length=533, Percent_Identity=26.6416510318949, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786717, Length=542, Percent_Identity=28.0442804428044, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI1787096, Length=525, Percent_Identity=24.5714285714286, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=26.6187050359712, Blast_Score=122, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=539, Percent_Identity=25.7884972170686, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=539, Percent_Identity=25.7884972170686, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=551, Percent_Identity=23.5934664246824, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=28.8732394366197, Blast_Score=196, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6320816, Length=553, Percent_Identity=21.6998191681736, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323073, Length=494, Percent_Identity=25.3036437246964, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=497, Percent_Identity=24.748490945674, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=494, Percent_Identity=24.6963562753036, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922626, Length=546, Percent_Identity=26.5567765567766, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59667; Mature: 59536

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKGSDLFVAALENEGVDRIFGIPGEENLDIVESIRRSSIQLILTRHEQAAAFMAATYGR
CCCCCCEEEEEECCCCCHHEECCCCCCCCHHHHHHHHCCEEEEEECCHHHHHHHHHHHHH
LTGKAGVCITTLGPGALNLTTGAAYALLGAMPMIMITGQKGILSSRQARFQIVDIVAAMK
CCCCCCEEEEECCCCCEEECHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
PLTKLSRQIVSPKMIPSLVREAFRVAQEERPGPVHLELPEDIAAAECEPVALVPTHPVEL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCEEEECCCCCCC
PLASPGALDRAARMIMEAKHPLLMFGAAASRPRVTPDVAQFVLRTQIPYFTTQMGKGTVP
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHEECCCCCCCC
GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTVEKPPFIMGANGPKVIHVGYHSADV
CCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCC
EQVYFPQAEIVGDLGPSLALLADRIEGKIPNAQALLPLRENILSRIAARATEDRFTPQRI
HHEECCHHHHHHCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHH
VHDVRAVMPADGILALDNGMYKIWFARNYRTRMANTLLLDNALATMGAGLPSAMMASLLF
HHHHHHHCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
PTRRVMAICGDGGFMMNSQELETAVRLKLNLVILLLEDHAYGMIRWKQAVDEFPDFGMTF
CCCEEEEEECCCCEEECHHHHHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
GNPDFVKYAEAYGAKGTRVTEIADLRPALERAFTGGGVHLVVVPIDYSENTRVLVDELRE
CCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHH
RLPAPRTP
HCCCCCCC
>Mature Secondary Structure 
TKGSDLFVAALENEGVDRIFGIPGEENLDIVESIRRSSIQLILTRHEQAAAFMAATYGR
CCCCCEEEEEECCCCCHHEECCCCCCCCHHHHHHHHCCEEEEEECCHHHHHHHHHHHHH
LTGKAGVCITTLGPGALNLTTGAAYALLGAMPMIMITGQKGILSSRQARFQIVDIVAAMK
CCCCCCEEEEECCCCCEEECHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
PLTKLSRQIVSPKMIPSLVREAFRVAQEERPGPVHLELPEDIAAAECEPVALVPTHPVEL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCEEEECCCCCCC
PLASPGALDRAARMIMEAKHPLLMFGAAASRPRVTPDVAQFVLRTQIPYFTTQMGKGTVP
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHEECCCCCCCC
GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTVEKPPFIMGANGPKVIHVGYHSADV
CCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCC
EQVYFPQAEIVGDLGPSLALLADRIEGKIPNAQALLPLRENILSRIAARATEDRFTPQRI
HHEECCHHHHHHCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHH
VHDVRAVMPADGILALDNGMYKIWFARNYRTRMANTLLLDNALATMGAGLPSAMMASLLF
HHHHHHHCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
PTRRVMAICGDGGFMMNSQELETAVRLKLNLVILLLEDHAYGMIRWKQAVDEFPDFGMTF
CCCEEEEEECCCCEEECHHHHHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
GNPDFVKYAEAYGAKGTRVTEIADLRPALERAFTGGGVHLVVVPIDYSENTRVLVDELRE
CCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHH
RLPAPRTP
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]