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The map label for this gene is alkJ [H]

Identifier: 13475914

GI number: 13475914

Start: 5858250

End: 5859863

Strand: Direct

Name: alkJ [H]

Synonym: mlr7098

Alternate gene names: 13475914

Gene position: 5858250-5859863 (Clockwise)

Preceding gene: 13475913

Following gene: 13475915

Centisome position: 83.26

GC content: 64.81

Gene sequence:

>1614_bases
GTGAGCGAAACTTCCCCTTTCGGCGATTACGACTACGTCATCGTCGGTGCCGGCTCCGCCGGCTGCGTGCTCGCCAACCG
GCTCTCGGCCGATCCGCGCAACCGGGTGCTGCTCCTCGAAGCCGGCGGCAGCGACCGCTATCACTGGGTCGATATCCCGA
TCGGCTACCTCTTCTGCATGGGCAATCCGCGCACCGACTGGATGATGAAGACCGAGCCGGAAGCCGGTCTCAACGGACGC
AGCCTCAACTATCCGCGTGGCAAGGTGCTTGGCGGCTGTTCGTCGGTCAACGGCATGATCTACATGCGCGGCCAGGCAGC
CGACTATGACGGCTGGCGACAGGCCGGCAATGTCGGCTGGAGCTGGGACGATGTGCTGCCGTATTTCCGCAAGTCGGAAG
ACTATCACGGTGGCAAGAGCGCCATGCACGGCAGCGGCGGCGAGTGGCGGGTCGAACGCCAGCGCCTGTCCTGGCCGATC
CTCGACGCGTTCCGGGACGCCGCCGAGGAACTCGGCATTCCCCGCACAGGGGATTTCAACACCGGCACCAATGAAGGCTC
GGGCTATTTCGAGGTCAACCAGCGGCTGGGCGTGCGCTGGAACACATCGAAGGCGTTTTTGCGCGGCATTGCGGCGCGTC
AGAATCTCCGCGTGGTGACGGGGGCAGAGACGGAACGGCTCGAATTCGACGGCCGGCGCGTCACCGGACTCCTCTTTCGC
CAGGGCGACCGCCTGCACAGGGTCAGCGCCGGACAGACGATCCTTGCGGCCGGTGCCATCAACTCGCCGAAGATCCTCGA
ACTGTCGGGGGTGGGGCGACCCGACCTGCTTTCGAGCATTGGCGTGGATGTTCGACACGCCTTGCCCGGCGTTGGCGAAA
ACCTGCAGGACCATCTGCAGATACGCACGGTTTTCAAGGTCGCCAATGCCGCGACGCTCAACGAGCGCTATCACAATCTG
ATCAGCCGCGCGGCGATGGGTGTGGAATACGCGCTCAAACGCAGCGGGCCGCTGTCAATGGCGCCGAGCCAGCTCGGCAT
CTTCGCCAGAAGCGATGCTCGGCTGGCAACACCTGATCTGGAATACCACGTGCAACCGCTGAGCACCGACCGTCTGGGCG
AGCCGCTGCATCGCTTTCCCGCGGTCACCGTGTCGGTCTGCAATCTGCGCCCGGAAAGTCGGGGCACCGTGCATATGGCA
TCGTCCGATCCGCGCGAGGCGACGAAGATCCGGCCGAACTATCTCTCCACCGCGGGAGACCGCAGCGTCGCGGTCGCATC
GCTGCGCCATGTCCGCAGCCTGATGAAGGCGCGGGCCGTGGCGCGCTTTGCGCCCGAAGAGATGCTGCCCGGCACGCAGT
ATGACAGCGACGAAGACCTCATCCGCAAGGCCGGCGATATCGGCACGACGATCTTCCACCCCGTCGGCACCTGCAAGATG
GGTTCGGACTCGATGGCGGTGGTCGACGACCGGCTACGCGTTCACGGGCTTGCCGGACTGCGCGTGGTCGATGCCTCGAT
CATGCCGACAATCGTCTCGGGCAACACCAACTCTCCGGTCGTCATGATCGCCGAGAAGGCAGCGGAGATGATCCTGCTTG
ATCCGCGCCGATAA

Upstream 100 bases:

>100_bases
CGGTTGCCGAGGCTTACGCGGGAGGGTATCGCCTGTATGGCGAGATCAACCAGGCGATGATTCCTATGTGGGGGCGCATC
GCCGCACGGCAGGCACGCAC

Downstream 100 bases:

>100_bases
TGTCGTGGCGATATGGGGCGTCGTCGGATGCCGAAGCCCCCGCCATCGTTTCGCGCGCGAGCTGGACGATCACGATATCG
GTGATGCACGGCCGTTCCGG

Product: GMC-type oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSETSPFGDYDYVIVGAGSAGCVLANRLSADPRNRVLLLEAGGSDRYHWVDIPIGYLFCMGNPRTDWMMKTEPEAGLNGR
SLNYPRGKVLGGCSSVNGMIYMRGQAADYDGWRQAGNVGWSWDDVLPYFRKSEDYHGGKSAMHGSGGEWRVERQRLSWPI
LDAFRDAAEELGIPRTGDFNTGTNEGSGYFEVNQRLGVRWNTSKAFLRGIAARQNLRVVTGAETERLEFDGRRVTGLLFR
QGDRLHRVSAGQTILAAGAINSPKILELSGVGRPDLLSSIGVDVRHALPGVGENLQDHLQIRTVFKVANAATLNERYHNL
ISRAAMGVEYALKRSGPLSMAPSQLGIFARSDARLATPDLEYHVQPLSTDRLGEPLHRFPAVTVSVCNLRPESRGTVHMA
SSDPREATKIRPNYLSTAGDRSVAVASLRHVRSLMKARAVARFAPEEMLPGTQYDSDEDLIRKAGDIGTTIFHPVGTCKM
GSDSMAVVDDRLRVHGLAGLRVVDASIMPTIVSGNTNSPVVMIAEKAAEMILLDPRR

Sequences:

>Translated_537_residues
MSETSPFGDYDYVIVGAGSAGCVLANRLSADPRNRVLLLEAGGSDRYHWVDIPIGYLFCMGNPRTDWMMKTEPEAGLNGR
SLNYPRGKVLGGCSSVNGMIYMRGQAADYDGWRQAGNVGWSWDDVLPYFRKSEDYHGGKSAMHGSGGEWRVERQRLSWPI
LDAFRDAAEELGIPRTGDFNTGTNEGSGYFEVNQRLGVRWNTSKAFLRGIAARQNLRVVTGAETERLEFDGRRVTGLLFR
QGDRLHRVSAGQTILAAGAINSPKILELSGVGRPDLLSSIGVDVRHALPGVGENLQDHLQIRTVFKVANAATLNERYHNL
ISRAAMGVEYALKRSGPLSMAPSQLGIFARSDARLATPDLEYHVQPLSTDRLGEPLHRFPAVTVSVCNLRPESRGTVHMA
SSDPREATKIRPNYLSTAGDRSVAVASLRHVRSLMKARAVARFAPEEMLPGTQYDSDEDLIRKAGDIGTTIFHPVGTCKM
GSDSMAVVDDRLRVHGLAGLRVVDASIMPTIVSGNTNSPVVMIAEKAAEMILLDPRR
>Mature_536_residues
SETSPFGDYDYVIVGAGSAGCVLANRLSADPRNRVLLLEAGGSDRYHWVDIPIGYLFCMGNPRTDWMMKTEPEAGLNGRS
LNYPRGKVLGGCSSVNGMIYMRGQAADYDGWRQAGNVGWSWDDVLPYFRKSEDYHGGKSAMHGSGGEWRVERQRLSWPIL
DAFRDAAEELGIPRTGDFNTGTNEGSGYFEVNQRLGVRWNTSKAFLRGIAARQNLRVVTGAETERLEFDGRRVTGLLFRQ
GDRLHRVSAGQTILAAGAINSPKILELSGVGRPDLLSSIGVDVRHALPGVGENLQDHLQIRTVFKVANAATLNERYHNLI
SRAAMGVEYALKRSGPLSMAPSQLGIFARSDARLATPDLEYHVQPLSTDRLGEPLHRFPAVTVSVCNLRPESRGTVHMAS
SDPREATKIRPNYLSTAGDRSVAVASLRHVRSLMKARAVARFAPEEMLPGTQYDSDEDLIRKAGDIGTTIFHPVGTCKMG
SDSMAVVDDRLRVHGLAGLRVVDASIMPTIVSGNTNSPVVMIAEKAAEMILLDPRR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=540, Percent_Identity=39.8148148148148, Blast_Score=371, Evalue=1e-102,
Organism=Escherichia coli, GI1786503, Length=530, Percent_Identity=39.2452830188679, Blast_Score=347, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17532301, Length=545, Percent_Identity=36.697247706422, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24642055, Length=573, Percent_Identity=37.696335078534, Blast_Score=307, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24642048, Length=577, Percent_Identity=36.0485268630849, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI45551458, Length=569, Percent_Identity=36.2038664323374, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI45549471, Length=569, Percent_Identity=36.2038664323374, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI24642059, Length=576, Percent_Identity=35.5902777777778, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24642042, Length=579, Percent_Identity=35.405872193437, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI18859995, Length=573, Percent_Identity=36.1256544502618, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24642051, Length=571, Percent_Identity=34.6760070052539, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI17137792, Length=570, Percent_Identity=35.9649122807018, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642039, Length=579, Percent_Identity=35.405872193437, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24650267, Length=565, Percent_Identity=33.9823008849557, Blast_Score=265, Evalue=8e-71,
Organism=Drosophila melanogaster, GI18859993, Length=568, Percent_Identity=32.2183098591549, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24642037, Length=572, Percent_Identity=31.8181818181818, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24642035, Length=305, Percent_Identity=38.0327868852459, Blast_Score=195, Evalue=6e-50,
Organism=Drosophila melanogaster, GI24642057, Length=584, Percent_Identity=28.7671232876712, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24645930, Length=576, Percent_Identity=28.8194444444444, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58747; Mature: 58616

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETSPFGDYDYVIVGAGSAGCVLANRLSADPRNRVLLLEAGGSDRYHWVDIPIGYLFCM
CCCCCCCCCCCEEEEECCCCCEEEEHHCCCCCCCCEEEEECCCCCCEEEEEECEEEEEEC
GNPRTDWMMKTEPEAGLNGRSLNYPRGKVLGGCSSVNGMIYMRGQAADYDGWRQAGNVGW
CCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHCCCCCC
SWDDVLPYFRKSEDYHGGKSAMHGSGGEWRVERQRLSWPILDAFRDAAEELGIPRTGDFN
CHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHCCCCCCCCCC
TGTNEGSGYFEVNQRLGVRWNTSKAFLRGIAARQNLRVVTGAETERLEFDGRRVTGLLFR
CCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCCCEEEEECCCCEEEEECCCEEEEEEEC
QGDRLHRVSAGQTILAAGAINSPKILELSGVGRPDLLSSIGVDVRHALPGVGENLQDHLQ
CCCCEEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHHCCCHHHHHCCCCCCCHHHHHH
IRTVFKVANAATLNERYHNLISRAAMGVEYALKRSGPLSMAPSQLGIFARSDARLATPDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEECCCEEECCCC
EYHVQPLSTDRLGEPLHRFPAVTVSVCNLRPESRGTVHMASSDPREATKIRPNYLSTAGD
EEEEEECCCHHCCCHHHHCCCEEEEEEECCCCCCCEEEECCCCCHHHHHCCCCHHCCCCC
RSVAVASLRHVRSLMKARAVARFAPEEMLPGTQYDSDEDLIRKAGDIGTTIFHPVGTCKM
CHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCCCEEE
GSDSMAVVDDRLRVHGLAGLRVVDASIMPTIVSGNTNSPVVMIAEKAAEMILLDPRR
CCCCEEEECCCCEEECCCCEEEECCCCCCEEEECCCCCCEEEEEECCCCEEEECCCC
>Mature Secondary Structure 
SETSPFGDYDYVIVGAGSAGCVLANRLSADPRNRVLLLEAGGSDRYHWVDIPIGYLFCM
CCCCCCCCCCEEEEECCCCCEEEEHHCCCCCCCCEEEEECCCCCCEEEEEECEEEEEEC
GNPRTDWMMKTEPEAGLNGRSLNYPRGKVLGGCSSVNGMIYMRGQAADYDGWRQAGNVGW
CCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHCCCCCC
SWDDVLPYFRKSEDYHGGKSAMHGSGGEWRVERQRLSWPILDAFRDAAEELGIPRTGDFN
CHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHCCCCCCCCCC
TGTNEGSGYFEVNQRLGVRWNTSKAFLRGIAARQNLRVVTGAETERLEFDGRRVTGLLFR
CCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCCCEEEEECCCCEEEEECCCEEEEEEEC
QGDRLHRVSAGQTILAAGAINSPKILELSGVGRPDLLSSIGVDVRHALPGVGENLQDHLQ
CCCCEEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHHCCCHHHHHCCCCCCCHHHHHH
IRTVFKVANAATLNERYHNLISRAAMGVEYALKRSGPLSMAPSQLGIFARSDARLATPDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEECCCEEECCCC
EYHVQPLSTDRLGEPLHRFPAVTVSVCNLRPESRGTVHMASSDPREATKIRPNYLSTAGD
EEEEEECCCHHCCCHHHHCCCEEEEEEECCCCCCCEEEECCCCCHHHHHCCCCHHCCCCC
RSVAVASLRHVRSLMKARAVARFAPEEMLPGTQYDSDEDLIRKAGDIGTTIFHPVGTCKM
CHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCCCEEE
GSDSMAVVDDRLRVHGLAGLRVVDASIMPTIVSGNTNSPVVMIAEKAAEMILLDPRR
CCCCEEEECCCCEEECCCCEEEECCCCCCEEEECCCCCCEEEEEECCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]