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The map label for this gene is apbE [H]

Identifier: 13475525

GI number: 13475525

Start: 5421311

End: 5422054

Strand: Reverse

Name: apbE [H]

Synonym: mll6620

Alternate gene names: 13475525

Gene position: 5422054-5421311 (Counterclockwise)

Preceding gene: 13475526

Following gene: 13475524

Centisome position: 77.06

GC content: 60.48

Gene sequence:

>744_bases
ATGCGCGACACGCGAATTTTGATGGGCATGCCCATCACAGTCGACATCGGTGGCACGTCGACCGATGATCTGATCGACAC
GGTCTTCGGCTATTTCGAACATATCGACCGGCGCTTCAGCACGTACCGGGCCGACAGCGAGATCGCGGCCATCAACCGCG
GCGACGTTCCGGTCGTCGACTGGAGCGGCGAGATGATGGAAGTATTCGCTCTCGCCCAACAGACGAAAGACCAGACGGAC
GGATATTTCGACATTCGCAAGCCCGACGGGTCGCTCGATCCTTCCGGCATCGTCAAGGGCTGGGCCATCCGCAATGCGGC
AGGGATTGTTCAACGGGCCGGCATCAGTGATTTCTTCATCGAGGCGGGCGGCGATATCCAGTCCTGCGGCAAGAATGCAT
CGGGCCATGACTGGAGCGTCGGCATCCGCAATCCCTTCAATACGGACGAGATCGTAAAGATCGTGTATCCCCGCGGTCGC
GGTGTCGCAACCTCCGGCACCTATGTCCGCGGTCAACACATTTACAATCCGCATGGAATTGGCCATCCGATCCAGGACGT
CGTCAGCCTGACCGTGATCGGCGCCGATGTGCTCGAAGCCGATCGTTTCGCCACCGCGGCCTTCGCCATGGGGCGGGATG
GCATTCTCTTCATCGAGCAGACGCTAGGCCTTGAGGGCTATCTCGTCGACGCCAATGGCCGCGCTACGCCGACAACCGGG
TTCGGAGCCCTTTGCCTGCCATGA

Upstream 100 bases:

>100_bases
CAGAGCGCCGATGTCGATATTATTTCGGGCGCGACGCTGACCAGCAGGGCTTTCATCCAGTCGCTGCGCGGCGCGCTGAA
GAAAGCGTCGTCGTAGGTCC

Downstream 100 bases:

>100_bases
TCAAGACCATCGACCGATTTCTCGATCACCTGACGATGTACAGGCTGGTCCTCTACTATCTGATGGCGCTGGTCGGCGCG
GCGTTCGTTCTCGGCTTCGT

Product: thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRDTRILMGMPITVDIGGTSTDDLIDTVFGYFEHIDRRFSTYRADSEIAAINRGDVPVVDWSGEMMEVFALAQQTKDQTD
GYFDIRKPDGSLDPSGIVKGWAIRNAAGIVQRAGISDFFIEAGGDIQSCGKNASGHDWSVGIRNPFNTDEIVKIVYPRGR
GVATSGTYVRGQHIYNPHGIGHPIQDVVSLTVIGADVLEADRFATAAFAMGRDGILFIEQTLGLEGYLVDANGRATPTTG
FGALCLP

Sequences:

>Translated_247_residues
MRDTRILMGMPITVDIGGTSTDDLIDTVFGYFEHIDRRFSTYRADSEIAAINRGDVPVVDWSGEMMEVFALAQQTKDQTD
GYFDIRKPDGSLDPSGIVKGWAIRNAAGIVQRAGISDFFIEAGGDIQSCGKNASGHDWSVGIRNPFNTDEIVKIVYPRGR
GVATSGTYVRGQHIYNPHGIGHPIQDVVSLTVIGADVLEADRFATAAFAMGRDGILFIEQTLGLEGYLVDANGRATPTTG
FGALCLP
>Mature_247_residues
MRDTRILMGMPITVDIGGTSTDDLIDTVFGYFEHIDRRFSTYRADSEIAAINRGDVPVVDWSGEMMEVFALAQQTKDQTD
GYFDIRKPDGSLDPSGIVKGWAIRNAAGIVQRAGISDFFIEAGGDIQSCGKNASGHDWSVGIRNPFNTDEIVKIVYPRGR
GVATSGTYVRGQHIYNPHGIGHPIQDVVSLTVIGADVLEADRFATAAFAMGRDGILFIEQTLGLEGYLVDANGRATPTTG
FGALCLP

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=162, Percent_Identity=30.8641975308642, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 26678; Mature: 26678

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDTRILMGMPITVDIGGTSTDDLIDTVFGYFEHIDRRFSTYRADSEIAAINRGDVPVVD
CCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEE
WSGEMMEVFALAQQTKDQTDGYFDIRKPDGSLDPSGIVKGWAIRNAAGIVQRAGISDFFI
CCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEECHHHHHHHHHHHHCCCCEEEE
EAGGDIQSCGKNASGHDWSVGIRNPFNTDEIVKIVYPRGRGVATSGTYVRGQHIYNPHGI
ECCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCEECCCCEEECCEEECCCCC
GHPIQDVVSLTVIGADVLEADRFATAAFAMGRDGILFIEQTLGLEGYLVDANGRATPTTG
CCCHHHHHEEHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCC
FGALCLP
CCEEECC
>Mature Secondary Structure
MRDTRILMGMPITVDIGGTSTDDLIDTVFGYFEHIDRRFSTYRADSEIAAINRGDVPVVD
CCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEE
WSGEMMEVFALAQQTKDQTDGYFDIRKPDGSLDPSGIVKGWAIRNAAGIVQRAGISDFFI
CCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEECHHHHHHHHHHHHCCCCEEEE
EAGGDIQSCGKNASGHDWSVGIRNPFNTDEIVKIVYPRGRGVATSGTYVRGQHIYNPHGI
ECCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCEECCCCEEECCEEECCCCC
GHPIQDVVSLTVIGADVLEADRFATAAFAMGRDGILFIEQTLGLEGYLVDANGRATPTTG
CCCHHHHHEEHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCC
FGALCLP
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]