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The map label for this gene is phoQ [H]

Identifier: 13475523

GI number: 13475523

Start: 5418452

End: 5419795

Strand: Direct

Name: phoQ [H]

Synonym: mlr6618

Alternate gene names: 13475523

Gene position: 5418452-5419795 (Clockwise)

Preceding gene: 13475522

Following gene: 13475527

Centisome position: 77.01

GC content: 61.9

Gene sequence:

>1344_bases
ATGGGCAACTCGATCCGCTTCAGGCTCTGGTTGGCCGCAACGATCTCGATCCTTGTTGCTCTTGCCATCGCCGGTGTCGG
ACTGCGCTATCTGTTCGAGCTGAACGTCGAGCGGCGCGTTGTCAGCGAGCTGACAGTCGACCTGAACGACCTTATCGGCG
CGACCAGTTTCGCCGCCGATGGCCGTCTTTCGGTCCAGGCGGCTTTGACCGACCCACGGTTCTCCATTCCCTTTTCCGGC
CACTACTGGCAGGTGCAGGATACTGGTTCCGGCAACCTTGTCCGCTCGCGTTCATTGTGGGATGCGACGCTTGCCTTGCC
TGATCACGCAACCAACGGCGAACTCAGGGAGATCAAGGAATTCAAGGGGCCACAAGGGGAACTCACCATCGCCGTCGAGC
GAACCATCATCGACGCCAGTGGCCGATCGTTTCGCGCCGTCGTGGCTGAAGACCATCAAACGGTGAAGGAATCCGTCGAC
GGGTATGTCCGCGACCTTGCGCCAGCCCTCATTTTGCTCGCCATTGCTCTCATGGCGGCCTTCTTCATCCAGATCACCGT
CGGTCTCGCGCCTCTGGAAACCTTGCGGGTCTCGGTGAGAAACGTGATTGCACAGCGAACGGCACGGCTCGAGGTGGCGG
CACCAAGCGAAGTCCAGCCGCTCGCGGACGAAATCAATCGCCTTCTCGATGCCCAGGAGAAGGCACTTGCTCGAGCCCGA
TCGCGCGCCACAGATCTGGCGCATGGTCTCAAGACACCGCTGCAAGTGCTGTCGGCCGATATCCGCGCGTTGAGAAAAAA
AGGCGAAACGGGGCTGGCCGATGAGATCGAGAAGAGCGCCGCTGCGATCCGGCGCCATGTCGAGCGGGAGCTTGCGCGCG
CCCGACTCGCGCCCGGCGTCTCCGGCAAGGCATCCTGCCTTGTGGCGGACGTCGCGGCTGGCGTCATTGCCGTCGTCAAG
CGGACACCAAGCGGCAAGCAGCTTTCATTCAATATGGATGTTGCGGAAGATGCGACGGCGCCAATCGACGAAGGCGATCT
GTCCGAGATCCTCGGCAATCTGGTCGAGAACGCCGCCCGCTACGCAAAATCGTCAGTCCGTGTCGGTGCATCCGCCGCCG
CAGGCGAGGTGGTTATCACCGTCACCGATGATGGCCCAGGCATACCGGATGCCGACCGGGAGTTCGCGCTGTCACGTGGT
GTCCAGCTCGACAGCAAGGGCGGCAGCAGCGGTCTTGGCCTGGCCATCGTATCCGATATCGTCGAAGCCTATGGTGGACG
CCTTGCCATGGCCAACGCCGATCCGGGTCTGGTCGTGACCATTTCCTTGCCCCGACACAGTTGA

Upstream 100 bases:

>100_bases
CGGTCGAAGTGATCGTCGCTCGCTTGCGTCGCAAGCTTGGGAGTGACGTCATCGAAACGCGGCGCGGATTCGGGTACTTC
GTCCCAGACGATTTCAGCTG

Downstream 100 bases:

>100_bases
GCATGCTTCAGGCAAAACCCGGGAAATAGTCGACCTTTATCTTCGGGCGGCGAACGCCCATGGCCAGCAGCTTCTGGCGC
AGCGCCTTGACCATCGCCTG

Product: two-component sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MGNSIRFRLWLAATISILVALAIAGVGLRYLFELNVERRVVSELTVDLNDLIGATSFAADGRLSVQAALTDPRFSIPFSG
HYWQVQDTGSGNLVRSRSLWDATLALPDHATNGELREIKEFKGPQGELTIAVERTIIDASGRSFRAVVAEDHQTVKESVD
GYVRDLAPALILLAIALMAAFFIQITVGLAPLETLRVSVRNVIAQRTARLEVAAPSEVQPLADEINRLLDAQEKALARAR
SRATDLAHGLKTPLQVLSADIRALRKKGETGLADEIEKSAAAIRRHVERELARARLAPGVSGKASCLVADVAAGVIAVVK
RTPSGKQLSFNMDVAEDATAPIDEGDLSEILGNLVENAARYAKSSVRVGASAAAGEVVITVTDDGPGIPDADREFALSRG
VQLDSKGGSSGLGLAIVSDIVEAYGGRLAMANADPGLVVTISLPRHS

Sequences:

>Translated_447_residues
MGNSIRFRLWLAATISILVALAIAGVGLRYLFELNVERRVVSELTVDLNDLIGATSFAADGRLSVQAALTDPRFSIPFSG
HYWQVQDTGSGNLVRSRSLWDATLALPDHATNGELREIKEFKGPQGELTIAVERTIIDASGRSFRAVVAEDHQTVKESVD
GYVRDLAPALILLAIALMAAFFIQITVGLAPLETLRVSVRNVIAQRTARLEVAAPSEVQPLADEINRLLDAQEKALARAR
SRATDLAHGLKTPLQVLSADIRALRKKGETGLADEIEKSAAAIRRHVERELARARLAPGVSGKASCLVADVAAGVIAVVK
RTPSGKQLSFNMDVAEDATAPIDEGDLSEILGNLVENAARYAKSSVRVGASAAAGEVVITVTDDGPGIPDADREFALSRG
VQLDSKGGSSGLGLAIVSDIVEAYGGRLAMANADPGLVVTISLPRHS
>Mature_446_residues
GNSIRFRLWLAATISILVALAIAGVGLRYLFELNVERRVVSELTVDLNDLIGATSFAADGRLSVQAALTDPRFSIPFSGH
YWQVQDTGSGNLVRSRSLWDATLALPDHATNGELREIKEFKGPQGELTIAVERTIIDASGRSFRAVVAEDHQTVKESVDG
YVRDLAPALILLAIALMAAFFIQITVGLAPLETLRVSVRNVIAQRTARLEVAAPSEVQPLADEINRLLDAQEKALARARS
RATDLAHGLKTPLQVLSADIRALRKKGETGLADEIEKSAAAIRRHVERELARARLAPGVSGKASCLVADVAAGVIAVVKR
TPSGKQLSFNMDVAEDATAPIDEGDLSEILGNLVENAARYAKSSVRVGASAAAGEVVITVTDDGPGIPDADREFALSRGV
QLDSKGGSSGLGLAIVSDIVEAYGGRLAMANADPGLVVTISLPRHS

Specific function: Member of the two-component regulatory system phoQ/phoP which regulates the expression of genes involved in virulence and resistance to host defense antimicrobial peptides. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=341, Percent_Identity=27.2727272727273, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=27.4021352313167, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790346, Length=246, Percent_Identity=28.4552845528455, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786783, Length=304, Percent_Identity=25, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=298, Percent_Identity=26.8456375838926, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790551, Length=241, Percent_Identity=29.4605809128631, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47383; Mature: 47251

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSIRFRLWLAATISILVALAIAGVGLRYLFELNVERRVVSELTVDLNDLIGATSFAAD
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHCCCC
GRLSVQAALTDPRFSIPFSGHYWQVQDTGSGNLVRSRSLWDATLALPDHATNGELREIKE
CCEEEEEEECCCCEECCCCCCEEEEEECCCCCEEECCCCCHHEEECCCCCCCCHHHHHHH
FKGPQGELTIAVERTIIDASGRSFRAVVAEDHQTVKESVDGYVRDLAPALILLAIALMAA
HCCCCCCEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFIQITVGLAPLETLRVSVRNVIAQRTARLEVAAPSEVQPLADEINRLLDAQEKALARAR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRATDLAHGLKTPLQVLSADIRALRKKGETGLADEIEKSAAAIRRHVERELARARLAPGV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SGKASCLVADVAAGVIAVVKRTPSGKQLSFNMDVAEDATAPIDEGDLSEILGNLVENAAR
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YAKSSVRVGASAAAGEVVITVTDDGPGIPDADREFALSRGVQLDSKGGSSGLGLAIVSDI
HHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHHHH
VEAYGGRLAMANADPGLVVTISLPRHS
HHHCCCEEEEECCCCCEEEEEECCCCC
>Mature Secondary Structure 
GNSIRFRLWLAATISILVALAIAGVGLRYLFELNVERRVVSELTVDLNDLIGATSFAAD
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHCCCC
GRLSVQAALTDPRFSIPFSGHYWQVQDTGSGNLVRSRSLWDATLALPDHATNGELREIKE
CCEEEEEEECCCCEECCCCCCEEEEEECCCCCEEECCCCCHHEEECCCCCCCCHHHHHHH
FKGPQGELTIAVERTIIDASGRSFRAVVAEDHQTVKESVDGYVRDLAPALILLAIALMAA
HCCCCCCEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFIQITVGLAPLETLRVSVRNVIAQRTARLEVAAPSEVQPLADEINRLLDAQEKALARAR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRATDLAHGLKTPLQVLSADIRALRKKGETGLADEIEKSAAAIRRHVERELARARLAPGV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SGKASCLVADVAAGVIAVVKRTPSGKQLSFNMDVAEDATAPIDEGDLSEILGNLVENAAR
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YAKSSVRVGASAAAGEVVITVTDDGPGIPDADREFALSRGVQLDSKGGSSGLGLAIVSDI
HHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHHHH
VEAYGGRLAMANADPGLVVTISLPRHS
HHHCCCEEEEECCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA