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The map label for this gene is fixJ [H]

Identifier: 13475513

GI number: 13475513

Start: 5408739

End: 5409353

Strand: Reverse

Name: fixJ [H]

Synonym: mll6606

Alternate gene names: 13475513

Gene position: 5409353-5408739 (Counterclockwise)

Preceding gene: 13475514

Following gene: 13475510

Centisome position: 76.88

GC content: 60.65

Gene sequence:

>615_bases
ATGGACGGCAGTGATCTGGTGCACGTGGTCGACGACGACGTGGACGTTCGCAAGTCACTCGGTTTTCTTCTGGCGACAGC
GGATTTTGCGGTGCGCTTGTACGAATCGGCGACGGCTTTTCTATCGACAGCGACGGGCAAGCTCGAGGGCTGCATCGTCA
CCGACGTACGCATGCCGGGCATAGACGGTATCGAGTTCCTGCGGCAGCTCAGAGCCAGCGGCCATACGATTCCGGTTATT
GTCATGACAGGCCATGCCGATGTGGCCCTCGCCGTCCAGGCGATGAAGGAGGGAGCCGCCGATTTCATCGAGAAGCCGTT
CGATGACGAGATGCTGATCGAAGCGATCCGTTCCGCATTGGCCAACCGCAACCAGGCACATGCGGCGCATCCCCAGTCCG
CGGATATCCGCGATCGTCTGTCCACCTTGTCGGAACGCGAGCGGCAGGTGCTGGATGGGCTCGTGTCTGGCCTGCCGAAC
AAGACGATCGCCTATGATCTGGGCATCAGCCCGCGCACCGTCGAAATCCACCGGGCCAATGTCATGAGCAAGATGGCAGC
AGGCAGCCTGTCACACCTCGTGCGCATGGCGCTTATCGTGGAGTCCAAATCCTAG

Upstream 100 bases:

>100_bases
GTTGAATCGCATGGCGGTCACATATGGGCCGAGGCAATCCCGGCCGGCGGGACGGCTTTCCGGTTCACCTTGCGCGTCGT
GGAGAAGGAAGAGGTCGCAA

Downstream 100 bases:

>100_bases
ACTTATCGGCCAGGCTGAAGTCCGTCGTCGTCGTTGAGCGTTCTCTCCATCGCCCAATCGCGCCAATGGGTCGGTCGGAT
ATCGACACGCGCGCCAGTGG

Product: response regulator FixJ

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MDGSDLVHVVDDDVDVRKSLGFLLATADFAVRLYESATAFLSTATGKLEGCIVTDVRMPGIDGIEFLRQLRASGHTIPVI
VMTGHADVALAVQAMKEGAADFIEKPFDDEMLIEAIRSALANRNQAHAAHPQSADIRDRLSTLSERERQVLDGLVSGLPN
KTIAYDLGISPRTVEIHRANVMSKMAAGSLSHLVRMALIVESKS

Sequences:

>Translated_204_residues
MDGSDLVHVVDDDVDVRKSLGFLLATADFAVRLYESATAFLSTATGKLEGCIVTDVRMPGIDGIEFLRQLRASGHTIPVI
VMTGHADVALAVQAMKEGAADFIEKPFDDEMLIEAIRSALANRNQAHAAHPQSADIRDRLSTLSERERQVLDGLVSGLPN
KTIAYDLGISPRTVEIHRANVMSKMAAGSLSHLVRMALIVESKS
>Mature_204_residues
MDGSDLVHVVDDDVDVRKSLGFLLATADFAVRLYESATAFLSTATGKLEGCIVTDVRMPGIDGIEFLRQLRASGHTIPVI
VMTGHADVALAVQAMKEGAADFIEKPFDDEMLIEAIRSALANRNQAHAAHPQSADIRDRLSTLSERERQVLDGLVSGLPN
KTIAYDLGISPRTVEIHRANVMSKMAAGSLSHLVRMALIVESKS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=120, Percent_Identity=34.1666666666667, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=32.6530612244898, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1786747, Length=160, Percent_Identity=28.75, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790437, Length=123, Percent_Identity=34.9593495934959, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788550, Length=112, Percent_Identity=33.9285714285714, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788521, Length=143, Percent_Identity=35.6643356643357, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788222, Length=142, Percent_Identity=27.4647887323944, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=32.2314049586777, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1790860, Length=125, Percent_Identity=26.4, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22036; Mature: 22036

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGSDLVHVVDDDVDVRKSLGFLLATADFAVRLYESATAFLSTATGKLEGCIVTDVRMPG
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
IDGIEFLRQLRASGHTIPVIVMTGHADVALAVQAMKEGAADFIEKPFDDEMLIEAIRSAL
CCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ANRNQAHAAHPQSADIRDRLSTLSERERQVLDGLVSGLPNKTIAYDLGISPRTVEIHRAN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEHHHHH
VMSKMAAGSLSHLVRMALIVESKS
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDGSDLVHVVDDDVDVRKSLGFLLATADFAVRLYESATAFLSTATGKLEGCIVTDVRMPG
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
IDGIEFLRQLRASGHTIPVIVMTGHADVALAVQAMKEGAADFIEKPFDDEMLIEAIRSAL
CCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ANRNQAHAAHPQSADIRDRLSTLSERERQVLDGLVSGLPNKTIAYDLGISPRTVEIHRAN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEHHHHH
VMSKMAAGSLSHLVRMALIVESKS
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]