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The map label for this gene is fixL [H]

Identifier: 13475514

GI number: 13475514

Start: 5409340

End: 5410938

Strand: Reverse

Name: fixL [H]

Synonym: mll6607

Alternate gene names: 13475514

Gene position: 5410938-5409340 (Counterclockwise)

Preceding gene: 13475517

Following gene: 13475513

Centisome position: 76.9

GC content: 58.72

Gene sequence:

>1599_bases
ATGATAGGTAGCCCGGTAGCGTCTCGAACCGGGGAATTTCAGGTGATCAAAGACGAAGCGCTCAGTTTGTCGCCCGGCGG
GACAACACTCAAATCATTGCTGTCGCGAATGTTCGATCAGGCTCCGGGATTTATAGCGCTCATGCGCGAGCCAGGTCACG
TTTTCGAACTGACCAATGCCGCCTACCAGCAACTGATCGGCAACAGGCAAGTGATTGGAAAGCCCGTGCGTGATGCCTTT
CCCGAGCTCGAAGGACATGAGTTTTTCGACCATCTGGACCAAGTGTACACGACCGGCGAGCCCTATAGGGGCCACGGGGT
GAAGGTTGGCCTTCGCAACACAACCGACGGCCCTGTCGAAGAGCGCATCCTTGATTTTGTCTATCAGCCGATCAAGGCTG
ACGATGGCCGTATCACCGCGATCTTCATTGAAGGCACGGATGTCAGCGAACTCTCATTTGCAAACGCCGCACTTCGGCTG
CGCGAGGACCATTTGCGATCCATCCTGGCAACAGTGCCGGACGCAATGGTCGTCATTGACGAACAGGGCCGGATCCAGTC
CTTCAGCACCGCTGCCGAAACGCTGTTCGGCTATCAGTCAAGCGAGGTCATTGGTCAAAACGTGAAAATGCTGATGCCCT
CGCCCTACCGCGACGAGCATGATGCCTATCTGCTTCGCTATCTGACGACGGGAGAGCGCCGGATCATCGGGCTCGGCCGC
ACCGTTACCGGCGCGCGCAAGGATGGCACGACATTCCCGATGGAGCTTTCCGTCGGCGAGATGCACCCGGGAACGGGACG
TTTCTTTACCGGCTTCTGCCGGGACCTCACCGAACGCCACCGGGCAGAAGCAAGAATCCAGGAGCAGCAGCAGGAACTGC
TGCACATGGCGCGGTTTACAGCACTCGGCGAAATGGCTTCGACGCTTGCTCATGAGATCAACCAGCCACTGACGGCGATC
ACGAACTACCTCAAAGGCAGTCGTCGACTGCTCGAGAAAAGCCAGGACGAGAATGCGCCTATGCTTCGGGATGCTGTCGA
AAGGGCAGCTGAGCAGGCATTGCGAGCAGGCGACGTCATCCGTCATCTGAGGGATTTTGTGGCCAGGGGCGAAAGCGAGC
GCCAGGTCGAACGATTGCCGGTTCTCATCGAGGAGGCCGCATCCCTGGCCCTGGTCGGCGCGAGGGAGATAAATCTCCTT
GTCAGCTACAAGCTTGACCCTGCCGCCGAACTGGTCTTGACCGATCGCATCCAGATCCAGCAGGTTCTGCTCAATCTGAT
GCGCAATGCGGTAGAGGCAATGCAAGGCTCGCCTCGCCGCGAATTGAAGGTCACAACTGTCGCCCGTGATGATGGAATGG
CCGAGGTGAGCGTGATCGATACCGGCCCAGGCCTTGCCCCGGAGGTCTCGGCGCAACTCTTCCAGCCATTTGTGACCACC
AAGAAGCACGGCATGGGTGTCGGGCTTTCGATTTGCCGTACCATCGTTGAATCGCATGGCGGTCACATATGGGCCGAGGC
AATCCCGGCCGGCGGGACGGCTTTCCGGTTCACCTTGCGCGTCGTGGAGAAGGAAGAGGTCGCAAATGGACGGCAGTGA

Upstream 100 bases:

>100_bases
GCATTCGCCAGCGTCCAATGCCTTGATGCGCGTCAAAGATCGCCAACTGCGTCTGCGCACGAAAACGTCGGCTCGATCCG
TTTTGCGTGTGCGGCGAGAA

Downstream 100 bases:

>100_bases
TCTGGTGCACGTGGTCGACGACGACGTGGACGTTCGCAAGTCACTCGGTTTTCTTCTGGCGACAGCGGATTTTGCGGTGC
GCTTGTACGAATCGGCGACG

Product: two-component, nitrogen fixation sensor protein fixL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MIGSPVASRTGEFQVIKDEALSLSPGGTTLKSLLSRMFDQAPGFIALMREPGHVFELTNAAYQQLIGNRQVIGKPVRDAF
PELEGHEFFDHLDQVYTTGEPYRGHGVKVGLRNTTDGPVEERILDFVYQPIKADDGRITAIFIEGTDVSELSFANAALRL
REDHLRSILATVPDAMVVIDEQGRIQSFSTAAETLFGYQSSEVIGQNVKMLMPSPYRDEHDAYLLRYLTTGERRIIGLGR
TVTGARKDGTTFPMELSVGEMHPGTGRFFTGFCRDLTERHRAEARIQEQQQELLHMARFTALGEMASTLAHEINQPLTAI
TNYLKGSRRLLEKSQDENAPMLRDAVERAAEQALRAGDVIRHLRDFVARGESERQVERLPVLIEEAASLALVGAREINLL
VSYKLDPAAELVLTDRIQIQQVLLNLMRNAVEAMQGSPRRELKVTTVARDDGMAEVSVIDTGPGLAPEVSAQLFQPFVTT
KKHGMGVGLSICRTIVESHGGHIWAEAIPAGGTAFRFTLRVVEKEEVANGRQ

Sequences:

>Translated_532_residues
MIGSPVASRTGEFQVIKDEALSLSPGGTTLKSLLSRMFDQAPGFIALMREPGHVFELTNAAYQQLIGNRQVIGKPVRDAF
PELEGHEFFDHLDQVYTTGEPYRGHGVKVGLRNTTDGPVEERILDFVYQPIKADDGRITAIFIEGTDVSELSFANAALRL
REDHLRSILATVPDAMVVIDEQGRIQSFSTAAETLFGYQSSEVIGQNVKMLMPSPYRDEHDAYLLRYLTTGERRIIGLGR
TVTGARKDGTTFPMELSVGEMHPGTGRFFTGFCRDLTERHRAEARIQEQQQELLHMARFTALGEMASTLAHEINQPLTAI
TNYLKGSRRLLEKSQDENAPMLRDAVERAAEQALRAGDVIRHLRDFVARGESERQVERLPVLIEEAASLALVGAREINLL
VSYKLDPAAELVLTDRIQIQQVLLNLMRNAVEAMQGSPRRELKVTTVARDDGMAEVSVIDTGPGLAPEVSAQLFQPFVTT
KKHGMGVGLSICRTIVESHGGHIWAEAIPAGGTAFRFTLRVVEKEEVANGRQ
>Mature_532_residues
MIGSPVASRTGEFQVIKDEALSLSPGGTTLKSLLSRMFDQAPGFIALMREPGHVFELTNAAYQQLIGNRQVIGKPVRDAF
PELEGHEFFDHLDQVYTTGEPYRGHGVKVGLRNTTDGPVEERILDFVYQPIKADDGRITAIFIEGTDVSELSFANAALRL
REDHLRSILATVPDAMVVIDEQGRIQSFSTAAETLFGYQSSEVIGQNVKMLMPSPYRDEHDAYLLRYLTTGERRIIGLGR
TVTGARKDGTTFPMELSVGEMHPGTGRFFTGFCRDLTERHRAEARIQEQQQELLHMARFTALGEMASTLAHEINQPLTAI
TNYLKGSRRLLEKSQDENAPMLRDAVERAAEQALRAGDVIRHLRDFVARGESERQVERLPVLIEEAASLALVGAREINLL
VSYKLDPAAELVLTDRIQIQQVLLNLMRNAVEAMQGSPRRELKVTTVARDDGMAEVSVIDTGPGLAPEVSAQLFQPFVTT
KKHGMGVGLSICRTIVESHGGHIWAEAIPAGGTAFRFTLRVVEKEEVANGRQ

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=385, Percent_Identity=30.3896103896104, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=30.2127659574468, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790300, Length=243, Percent_Identity=31.2757201646091, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788713, Length=387, Percent_Identity=22.4806201550388, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=28.0542986425339, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58856; Mature: 58856

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGSPVASRTGEFQVIKDEALSLSPGGTTLKSLLSRMFDQAPGFIALMREPGHVFELTNA
CCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHH
AYQQLIGNRQVIGKPVRDAFPELEGHEFFDHLDQVYTTGEPYRGHGVKVGLRNTTDGPVE
HHHHHHCCCHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHH
ERILDFVYQPIKADDGRITAIFIEGTDVSELSFANAALRLREDHLRSILATVPDAMVVID
HHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
EQGRIQSFSTAAETLFGYQSSEVIGQNVKMLMPSPYRDEHDAYLLRYLTTGERRIIGLGR
CCCCCHHHHHHHHHHHCCCCHHHHCCCCCEECCCCCCCCCHHHHHHHHHCCCEEEEEECC
TVTGARKDGTTFPMELSVGEMHPGTGRFFTGFCRDLTERHRAEARIQEQQQELLHMARFT
CCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGEMASTLAHEINQPLTAITNYLKGSRRLLEKSQDENAPMLRDAVERAAEQALRAGDVI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RHLRDFVARGESERQVERLPVLIEEAASLALVGAREINLLVSYKLDPAAELVLTDRIQIQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEEEEECCCCHHHHHHHHHHHHH
QVLLNLMRNAVEAMQGSPRRELKVTTVARDDGMAEVSVIDTGPGLAPEVSAQLFQPFVTT
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
KKHGMGVGLSICRTIVESHGGHIWAEAIPAGGTAFRFTLRVVEKEEVANGRQ
HCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEHHHHHHCCCCC
>Mature Secondary Structure
MIGSPVASRTGEFQVIKDEALSLSPGGTTLKSLLSRMFDQAPGFIALMREPGHVFELTNA
CCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHH
AYQQLIGNRQVIGKPVRDAFPELEGHEFFDHLDQVYTTGEPYRGHGVKVGLRNTTDGPVE
HHHHHHCCCHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHH
ERILDFVYQPIKADDGRITAIFIEGTDVSELSFANAALRLREDHLRSILATVPDAMVVID
HHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
EQGRIQSFSTAAETLFGYQSSEVIGQNVKMLMPSPYRDEHDAYLLRYLTTGERRIIGLGR
CCCCCHHHHHHHHHHHCCCCHHHHCCCCCEECCCCCCCCCHHHHHHHHHCCCEEEEEECC
TVTGARKDGTTFPMELSVGEMHPGTGRFFTGFCRDLTERHRAEARIQEQQQELLHMARFT
CCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGEMASTLAHEINQPLTAITNYLKGSRRLLEKSQDENAPMLRDAVERAAEQALRAGDVI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RHLRDFVARGESERQVERLPVLIEEAASLALVGAREINLLVSYKLDPAAELVLTDRIQIQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEEEEECCCCHHHHHHHHHHHHH
QVLLNLMRNAVEAMQGSPRRELKVTTVARDDGMAEVSVIDTGPGLAPEVSAQLFQPFVTT
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
KKHGMGVGLSICRTIVESHGGHIWAEAIPAGGTAFRFTLRVVEKEEVANGRQ
HCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]