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The map label for this gene is puuA [H]

Identifier: 13475449

GI number: 13475449

Start: 5329845

End: 5331281

Strand: Reverse

Name: puuA [H]

Synonym: mll6521

Alternate gene names: 13475449

Gene position: 5331281-5329845 (Counterclockwise)

Preceding gene: 13475451

Following gene: 13475448

Centisome position: 75.77

GC content: 62.98

Gene sequence:

>1437_bases
ATGATGCCGCCTGCAAAAAAGGAAGTCCGTCCGTCGAGCCGCGGAGGACGGGCGCGTACGCCTGCCTTCGTGAAGAACCT
GCGTGGTGTGAAGAACTGGAAGGAAGTCAGCGAGTGGCTGGAATGGCGCGGCATCGAGGACATCGAATGCATCACGCCTG
ATCAGGCGGGCGTGGCCCGCGGCAAGATGATGCCGTCGAAAAAATTCACCTCCAACACCTCGCTGGCGCTGCCTTCGGCC
GTTTTCATGACGACGATTTCCGGCGGCTATCCCGAAGACGGCAACGGCTTCCACTATCCCGAGGACGACGGCGACCTCAA
GCTGATGCCGGACCTGTCGACGCTGACAGTGGTGCCCTGGGAAGAAGACCCGACGGCCGCCGTCATCTGCGACCTCGTCC
ACCAGGACGGCCGCTCGGTAGAATTCACGCCGCGCAATGTGTTGAAGCGCGTGCTTGCCGCCTATGACAAGCTCGGGCTG
AGGCCGGTGGTGGCGCCCGAGATCGAGTTCTACCTGGTGCGCAAGAATCCGGACCCGGACTATCCGCTCACCCCGCCCGT
CGGTCGCTCGGGCCGTGCGATCGGCGGCGGCGCCGGCTATTCGATCGCCGGCGTCAACGAGTTCGACGAACTGATCGACG
ACATCTACCACTTCTCCGAAAGCCAGGGCCTGGAAATCGACACGCTCATCCATGAAGAGGGCGCCGGCCAGCTCGAGATC
AATCTGCGCCACGGCGACCCGATCGAGCTCGCCGACCAGGTGTTCATGTTCAAGCGCACCATCCGTGAAGCCGCGCTCAA
GCACGAAATCTACGCCACCTTCATGGCCAAGCCGATCCAGGGCCAGCCGGGTTCGGCCATGCATATCCACCAGTCGATCA
TCGACAAGAAGACCGGCAAGAATATCTTCTCGGCCGAGGACGGCTCGGAGACCGAGGCGTTCTTCCATTTCATCGGCGGC
ATGCAGAAACACGTGCCTAACGCGCTGGTGATGTTCGCGCCCTATGTCAATTCCTACCGGCGGCTGACGCAGGCGGCCTC
GGCTCCGGTCAACAACAAATGGGGCTATGACAACCGCACCACGGCGTTCCGTGTGCCGCGTTCGGATCCGGCGGCGCGGC
GTGTCGAAAACCGCATCCCGTCCTCCGATGCCAATCCCTACCTGGCGCTGGCGGCTTCGCTTGCCTGCGGGCTGATCGGC
ATCACCAGGAAGATCAAGGCCGAACCGCCGGTCCTGACGACCGCCAATGCGGACGAGATCGACCTGCCGCGCAGCCTGCT
CGAAGCCGTCGACCTGTTCGAAGGCGACGAGGAACTCTGCGCGCTGCTGGGCAAATCCTTCGCCGCCACCTATGCGGCGA
TCAAGCGGGCGGAATTCGAGACCTTCATGGAAGTGATCAGCCCGTGGGAGCGGGAGTACCTTCTGCTCAACGTGTGA

Upstream 100 bases:

>100_bases
GACGTGATTTTCAACCGGAACGAGGAATGCCGGCAGCACCTCCCTTCCCTTACACAGTGGCGCACGGCTATACTCCGAAA
TCCGCAACCCAATCTGGTGA

Downstream 100 bases:

>100_bases
GCGGCGGCTTCGGCGCGTGAATGGTGAACATTGAATGGTGAGTAGCTTCATACTGGATGGATGAGCGCAGACGGCGCGCT
TTCTCGCGGCCATTCACCAT

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MMPPAKKEVRPSSRGGRARTPAFVKNLRGVKNWKEVSEWLEWRGIEDIECITPDQAGVARGKMMPSKKFTSNTSLALPSA
VFMTTISGGYPEDGNGFHYPEDDGDLKLMPDLSTLTVVPWEEDPTAAVICDLVHQDGRSVEFTPRNVLKRVLAAYDKLGL
RPVVAPEIEFYLVRKNPDPDYPLTPPVGRSGRAIGGGAGYSIAGVNEFDELIDDIYHFSESQGLEIDTLIHEEGAGQLEI
NLRHGDPIELADQVFMFKRTIREAALKHEIYATFMAKPIQGQPGSAMHIHQSIIDKKTGKNIFSAEDGSETEAFFHFIGG
MQKHVPNALVMFAPYVNSYRRLTQAASAPVNNKWGYDNRTTAFRVPRSDPAARRVENRIPSSDANPYLALAASLACGLIG
ITRKIKAEPPVLTTANADEIDLPRSLLEAVDLFEGDEELCALLGKSFAATYAAIKRAEFETFMEVISPWEREYLLLNV

Sequences:

>Translated_478_residues
MMPPAKKEVRPSSRGGRARTPAFVKNLRGVKNWKEVSEWLEWRGIEDIECITPDQAGVARGKMMPSKKFTSNTSLALPSA
VFMTTISGGYPEDGNGFHYPEDDGDLKLMPDLSTLTVVPWEEDPTAAVICDLVHQDGRSVEFTPRNVLKRVLAAYDKLGL
RPVVAPEIEFYLVRKNPDPDYPLTPPVGRSGRAIGGGAGYSIAGVNEFDELIDDIYHFSESQGLEIDTLIHEEGAGQLEI
NLRHGDPIELADQVFMFKRTIREAALKHEIYATFMAKPIQGQPGSAMHIHQSIIDKKTGKNIFSAEDGSETEAFFHFIGG
MQKHVPNALVMFAPYVNSYRRLTQAASAPVNNKWGYDNRTTAFRVPRSDPAARRVENRIPSSDANPYLALAASLACGLIG
ITRKIKAEPPVLTTANADEIDLPRSLLEAVDLFEGDEELCALLGKSFAATYAAIKRAEFETFMEVISPWEREYLLLNV
>Mature_478_residues
MMPPAKKEVRPSSRGGRARTPAFVKNLRGVKNWKEVSEWLEWRGIEDIECITPDQAGVARGKMMPSKKFTSNTSLALPSA
VFMTTISGGYPEDGNGFHYPEDDGDLKLMPDLSTLTVVPWEEDPTAAVICDLVHQDGRSVEFTPRNVLKRVLAAYDKLGL
RPVVAPEIEFYLVRKNPDPDYPLTPPVGRSGRAIGGGAGYSIAGVNEFDELIDDIYHFSESQGLEIDTLIHEEGAGQLEI
NLRHGDPIELADQVFMFKRTIREAALKHEIYATFMAKPIQGQPGSAMHIHQSIIDKKTGKNIFSAEDGSETEAFFHFIGG
MQKHVPNALVMFAPYVNSYRRLTQAASAPVNNKWGYDNRTTAFRVPRSDPAARRVENRIPSSDANPYLALAASLACGLIG
ITRKIKAEPPVLTTANADEIDLPRSLLEAVDLFEGDEELCALLGKSFAATYAAIKRAEFETFMEVISPWEREYLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=422, Percent_Identity=27.2511848341232, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI87081870, Length=458, Percent_Identity=32.9694323144105, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1790301, Length=406, Percent_Identity=29.8029556650246, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 52926; Mature: 52926

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPPAKKEVRPSSRGGRARTPAFVKNLRGVKNWKEVSEWLEWRGIEDIECITPDQAGVAR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCHHHHHH
GKMMPSKKFTSNTSLALPSAVFMTTISGGYPEDGNGFHYPEDDGDLKLMPDLSTLTVVPW
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEC
EEDPTAAVICDLVHQDGRSVEFTPRNVLKRVLAAYDKLGLRPVVAPEIEFYLVRKNPDPD
CCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCC
YPLTPPVGRSGRAIGGGAGYSIAGVNEFDELIDDIYHFSESQGLEIDTLIHEEGAGQLEI
CCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEE
NLRHGDPIELADQVFMFKRTIREAALKHEIYATFMAKPIQGQPGSAMHIHQSIIDKKTGK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NIFSAEDGSETEAFFHFIGGMQKHVPNALVMFAPYVNSYRRLTQAASAPVNNKWGYDNRT
CCCCCCCCCHHHHHHHHHCCHHHHCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCC
TAFRVPRSDPAARRVENRIPSSDANPYLALAASLACGLIGITRKIKAEPPVLTTANADEI
EEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
DLPRSLLEAVDLFEGDEELCALLGKSFAATYAAIKRAEFETFMEVISPWEREYLLLNV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MMPPAKKEVRPSSRGGRARTPAFVKNLRGVKNWKEVSEWLEWRGIEDIECITPDQAGVAR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCHHHHHH
GKMMPSKKFTSNTSLALPSAVFMTTISGGYPEDGNGFHYPEDDGDLKLMPDLSTLTVVPW
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEC
EEDPTAAVICDLVHQDGRSVEFTPRNVLKRVLAAYDKLGLRPVVAPEIEFYLVRKNPDPD
CCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCC
YPLTPPVGRSGRAIGGGAGYSIAGVNEFDELIDDIYHFSESQGLEIDTLIHEEGAGQLEI
CCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEE
NLRHGDPIELADQVFMFKRTIREAALKHEIYATFMAKPIQGQPGSAMHIHQSIIDKKTGK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NIFSAEDGSETEAFFHFIGGMQKHVPNALVMFAPYVNSYRRLTQAASAPVNNKWGYDNRT
CCCCCCCCCHHHHHHHHHCCHHHHCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCC
TAFRVPRSDPAARRVENRIPSSDANPYLALAASLACGLIGITRKIKAEPPVLTTANADEI
EEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
DLPRSLLEAVDLFEGDEELCALLGKSFAATYAAIKRAEFETFMEVISPWEREYLLLNV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]