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The map label for this gene is metH [H]

Identifier: 13475184

GI number: 13475184

Start: 5023438

End: 5023656

Strand: Reverse

Name: metH [H]

Synonym: msl6205

Alternate gene names: 13475184

Gene position: 5023656-5023438 (Counterclockwise)

Preceding gene: 13475185

Following gene: 13475181

Centisome position: 71.4

GC content: 63.01

Gene sequence:

>219_bases
GTGAGCCTGACCGAAAGCTATGCGATGTGGCCCGGCTCGTCGGTCTCGGGGATCTACCTGTCACATCCCGAAAGCTATTA
TTTCGGCGTCGCCAAGGTCGAACGCGACCAGGTCGAGGACTATGCGCGCCGCAAGGCCATGCCGTTGGCCGAGGTCGAGC
GCTGGCTCGGCCCCATTCTCAACTGTGTGCCGGCACACTATGCCGAGGCGGCGGAATAG

Upstream 100 bases:

>100_bases
ACCTATCGCGGCATCCGCCCGGCTCCGGCTATCCGGCACAGCCCGACCACACCGAGAAGGCGACACTGTTCCGGCTGCTC
GACGGCGAGGCAATGCGGGC

Downstream 100 bases:

>100_bases
GGGCGGCAAGCTCTGTCGTCTCTTCGCTTTAACAGTGCTGGTGTTTTGCGCGCTACACTGTCATGCACGGCAAAGCCAGA
CCTGTCGAATCCTCATGTCG

Product: 5-methyltetrahydrofolate-homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS [H]

Number of amino acids: Translated: 72; Mature: 71

Protein sequence:

>72_residues
MSLTESYAMWPGSSVSGIYLSHPESYYFGVAKVERDQVEDYARRKAMPLAEVERWLGPILNCVPAHYAEAAE

Sequences:

>Translated_72_residues
MSLTESYAMWPGSSVSGIYLSHPESYYFGVAKVERDQVEDYARRKAMPLAEVERWLGPILNCVPAHYAEAAE
>Mature_71_residues
SLTESYAMWPGSSVSGIYLSHPESYYFGVAKVERDQVEDYARRKAMPLAEVERWLGPILNCVPAHYAEAAE

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=60, Percent_Identity=61.6666666666667, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790450, Length=60, Percent_Identity=70, Blast_Score=97, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17535405, Length=60, Percent_Identity=58.3333333333333, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 8125; Mature: 7994

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50974 ADOMET_ACTIVATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTESYAMWPGSSVSGIYLSHPESYYFGVAKVERDQVEDYARRKAMPLAEVERWLGPIL
CCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NCVPAHYAEAAE
HHHHHHHHCCCC
>Mature Secondary Structure 
SLTESYAMWPGSSVSGIYLSHPESYYFGVAKVERDQVEDYARRKAMPLAEVERWLGPIL
CCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NCVPAHYAEAAE
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185137; 2668277; 8369296; 8265357; 9278503; 1593636; 7992050; 8939751 [H]