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The map label for this gene is metH [H]

Identifier: 13475185

GI number: 13475185

Start: 5023653

End: 5024924

Strand: Reverse

Name: metH [H]

Synonym: mll6206

Alternate gene names: 13475185

Gene position: 5024924-5023653 (Counterclockwise)

Preceding gene: 13475186

Following gene: 13475184

Centisome position: 71.42

GC content: 62.11

Gene sequence:

>1272_bases
ATGTGGGGCTTCGGAACAATGGAGTTCCTTGTTCTTCAAGGTCTTGGCGACCTTCTGGCCATTCGAATTCGCATCCGCGG
CGGTTCGATTGGCAATGTTCAGTTCGTCTGCCGTTTTGTGAATTTCTGGAAGATGGCTCAGGATAATAACCGCTTTGCCG
AACTTCTGGATCGCGTGGTAGCCAACCGGCTCGCAGATGCGGTCCAGCCAGATCTTGCATTTCCGTCGCTGCGTCATGCC
GGCGGCATCAATCTGGTAATATTTCCGGCTAAAGTTGTCGAAAGCGATAGCATCGAGGAGCACGACAATTACGGCGTCGA
CTTCATCGAGGCGACCGGCGAGATCACCGCGACGCTGCGCATGTCCATATTTCCGGCGGCGTCTCGAACCTCTCCTTCTC
GTTCCGCGCGCGAGGCCATGCACGCCGTGTTCCTCTACCATGCCATCCAGCACGGCATGGACATGGGCATCGTCAATGCC
GGCCAGCTTGCGGTCTACGACACGATCGAGCCGGAACTGCGGGAGGCCTGCGAGGATGTCGTGCTCAACCGCGTCCCCAA
GGCCGGCGGTACCGCCACCGAACGCATGCTGGAGATCGCCGAACGCTTCAAGGGCACCGCAGGCCAGGAGGCCAGGGAGC
GCGATCTCGCCTGGCGCGAATTGCCGGTCGAACAGCGCATCAGCCATGCGCTGGTCAATGGCATCACCGAATTCATCGAC
GCCGACACCGAGGAAGCGCGGCTGGCGGCCGAGCGCCCGCTATTCCAGGATGCGCAGGCGATGCTGAAGAAGATCATCGC
CGAGAAATGGTTCGCGCCACGCGGCGTCATCGGCTTCTGGCCGGCCAATGCCGTCGGTGACGATATCAGGCTGTTTACGG
ACGAGGCGCCATCGCAGGAATTGGCGACGTTCTTCACCTTGCGCCAGCAACTGACCAAGCGCGACGGCAAGGCCAATGTC
GCGCTGTCGGATTTCGTTGCCCCGTCGGACGGCGGCAAGCCCGACTACATCAGCGGCTTCATCGTCACCGCCGGTATCGA
GGTGGCGATCGCCGAGCGTTTTCGAACGGGCGCACGACGATTATTCCTCGATCATGGTCAAGGCGCTGGCCGACCGCTTC
GCCGAGGCCTTCGCCGAGCGCCTGGCGCCAGACGAACTGATCGGCGAGACCTATCGCGGCATCCGCCCGGCTCCGGCTAT
CCGGCACAGCCCGACCACACCGAGAAGGCGACACTGTTCCGGCTGCTCGACGGCGAGGCAATGCGGGCGTGA

Upstream 100 bases:

>100_bases
AACAGAACGGTTGGGTCCAAGCCATGCAGATGCATCATTAAGGCTGGTGATGGCGCACTTCCCCTTCTGCTGCTTGTAGA
GGTTGGCATGATGAGCTTTC

Downstream 100 bases:

>100_bases
GCCTGACCGAAAGCTATGCGATGTGGCCCGGCTCGTCGGTCTCGGGGATCTACCTGTCACATCCCGAAAGCTATTATTTC
GGCGTCGCCAAGGTCGAACG

Product: 5-methyltetrahydrofolate-homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MWGFGTMEFLVLQGLGDLLAIRIRIRGGSIGNVQFVCRFVNFWKMAQDNNRFAELLDRVVANRLADAVQPDLAFPSLRHA
GGINLVIFPAKVVESDSIEEHDNYGVDFIEATGEITATLRMSIFPAASRTSPSRSAREAMHAVFLYHAIQHGMDMGIVNA
GQLAVYDTIEPELREACEDVVLNRVPKAGGTATERMLEIAERFKGTAGQEARERDLAWRELPVEQRISHALVNGITEFID
ADTEEARLAAERPLFQDAQAMLKKIIAEKWFAPRGVIGFWPANAVGDDIRLFTDEAPSQELATFFTLRQQLTKRDGKANV
ALSDFVAPSDGGKPDYISGFIVTAGIEVAIAERFRTGARRLFLDHGQGAGRPLRRGLRRAPGARRTDRRDLSRHPPGSGY
PAQPDHTEKATLFRLLDGEAMRA

Sequences:

>Translated_423_residues
MWGFGTMEFLVLQGLGDLLAIRIRIRGGSIGNVQFVCRFVNFWKMAQDNNRFAELLDRVVANRLADAVQPDLAFPSLRHA
GGINLVIFPAKVVESDSIEEHDNYGVDFIEATGEITATLRMSIFPAASRTSPSRSAREAMHAVFLYHAIQHGMDMGIVNA
GQLAVYDTIEPELREACEDVVLNRVPKAGGTATERMLEIAERFKGTAGQEARERDLAWRELPVEQRISHALVNGITEFID
ADTEEARLAAERPLFQDAQAMLKKIIAEKWFAPRGVIGFWPANAVGDDIRLFTDEAPSQELATFFTLRQQLTKRDGKANV
ALSDFVAPSDGGKPDYISGFIVTAGIEVAIAERFRTGARRLFLDHGQGAGRPLRRGLRRAPGARRTDRRDLSRHPPGSGY
PAQPDHTEKATLFRLLDGEAMRA
>Mature_423_residues
MWGFGTMEFLVLQGLGDLLAIRIRIRGGSIGNVQFVCRFVNFWKMAQDNNRFAELLDRVVANRLADAVQPDLAFPSLRHA
GGINLVIFPAKVVESDSIEEHDNYGVDFIEATGEITATLRMSIFPAASRTSPSRSAREAMHAVFLYHAIQHGMDMGIVNA
GQLAVYDTIEPELREACEDVVLNRVPKAGGTATERMLEIAERFKGTAGQEARERDLAWRELPVEQRISHALVNGITEFID
ADTEEARLAAERPLFQDAQAMLKKIIAEKWFAPRGVIGFWPANAVGDDIRLFTDEAPSQELATFFTLRQQLTKRDGKANV
ALSDFVAPSDGGKPDYISGFIVTAGIEVAIAERFRTGARRLFLDHGQGAGRPLRRGLRRAPGARRTDRRDLSRHPPGSGY
PAQPDHTEKATLFRLLDGEAMRA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=180, Percent_Identity=46.6666666666667, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1790450, Length=177, Percent_Identity=46.3276836158192, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17535405, Length=208, Percent_Identity=40.8653846153846, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 46852; Mature: 46852

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50974 ADOMET_ACTIVATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGFGTMEFLVLQGLGDLLAIRIRIRGGSIGNVQFVCRFVNFWKMAQDNNRFAELLDRVV
CCCCHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ANRLADAVQPDLAFPSLRHAGGINLVIFPAKVVESDSIEEHDNYGVDFIEATGEITATLR
HHHHHHHHCCCCCCCCHHHCCCCEEEEECHHHHCCCCCCCCCCCCCEEEECCCCEEEEEE
MSIFPAASRTSPSRSAREAMHAVFLYHAIQHGMDMGIVNAGQLAVYDTIEPELREACEDV
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHH
VLNRVPKAGGTATERMLEIAERFKGTAGQEARERDLAWRELPVEQRISHALVNGITEFID
HHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHC
ADTEEARLAAERPLFQDAQAMLKKIIAEKWFAPRGVIGFWPANAVGDDIRLFTDEAPSQE
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCHHH
LATFFTLRQQLTKRDGKANVALSDFVAPSDGGKPDYISGFIVTAGIEVAIAERFRTGARR
HHHHHHHHHHHHHCCCCCCEEHHHCCCCCCCCCCCHHCCEEEECCHHHHHHHHHHHHHHH
LFLDHGQGAGRPLRRGLRRAPGARRTDRRDLSRHPPGSGYPAQPDHTEKATLFRLLDGEA
EEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHH
MRA
HCC
>Mature Secondary Structure
MWGFGTMEFLVLQGLGDLLAIRIRIRGGSIGNVQFVCRFVNFWKMAQDNNRFAELLDRVV
CCCCHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ANRLADAVQPDLAFPSLRHAGGINLVIFPAKVVESDSIEEHDNYGVDFIEATGEITATLR
HHHHHHHHCCCCCCCCHHHCCCCEEEEECHHHHCCCCCCCCCCCCCEEEECCCCEEEEEE
MSIFPAASRTSPSRSAREAMHAVFLYHAIQHGMDMGIVNAGQLAVYDTIEPELREACEDV
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHH
VLNRVPKAGGTATERMLEIAERFKGTAGQEARERDLAWRELPVEQRISHALVNGITEFID
HHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHC
ADTEEARLAAERPLFQDAQAMLKKIIAEKWFAPRGVIGFWPANAVGDDIRLFTDEAPSQE
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCHHH
LATFFTLRQQLTKRDGKANVALSDFVAPSDGGKPDYISGFIVTAGIEVAIAERFRTGARR
HHHHHHHHHHHHHCCCCCCEEHHHCCCCCCCCCCCHHCCEEEECCHHHHHHHHHHHHHHH
LFLDHGQGAGRPLRRGLRRAPGARRTDRRDLSRHPPGSGYPAQPDHTEKATLFRLLDGEA
EEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHH
MRA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA