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The map label for this gene is nepI [H]

Identifier: 13475166

GI number: 13475166

Start: 5004175

End: 5005602

Strand: Reverse

Name: nepI [H]

Synonym: mll6183

Alternate gene names: 13475166

Gene position: 5005602-5004175 (Counterclockwise)

Preceding gene: 13475174

Following gene: 13475163

Centisome position: 71.14

GC content: 62.46

Gene sequence:

>1428_bases
ATGACGCGCTTTCCGACGACGACATCGTCGTCCGGCCCAAGGAAAGGGCCATCGATTTGCGCGCCGCACCGTCACAAAAT
ATCGCGAAGCGATGAACATCCCCTCGTCCGTGCAATGCCGCAGGGAATGGCGCTTCTGGCGTTAATCCGGGTCAACGCAG
CAACCCGAGTTTGCAGCACATCCAAACTGGAGACCCTGTTGATGACCGAATCCGCTACGGGCTTCATGGACGGAGTTCTG
CATGACCCAAAACAGCCAGATCAACGGTCTGGTCCCGCATGGGGTGCAGTCATTTCGCTTGCCTTAGGCACCTTTGGGCT
TGTGACGGCAGAGTTTCTGCCTGCCAGCGTGCTGACGCCGCTCGCGCATGATCTCTCTATCACCGAGGGTGCAGTCGGAC
AGGCGCTGACAACGACCGCCATCGTGGGGGCGATCTCGGCGCCGACGATGGCCGTCATCACAAGGCCCCTGGACCGCAGG
ATCGTCATATGGGCGATGACGCTGCTGCTGATCCTGTCGAACGTTCTATCGGCCGTCGCGGGGTCGCTGCCGGTTCTCCT
GGCCGCCCGCGTCGTGCTCGGCATAGCACTCGGAGGGTTCTGGTCGATTTCGGCAGCGCTGGCGATGCGGCTCGTTCCAA
GTCACCTCCTGCGGCGCGCCATGTCGATCATCCTCACCGGCGTTACCTGCGCTACCGTTTGCGCGGCTCCAATCGGCGCC
TATGTCGGCGATATCTGGGGATGGCGAACCGCCTTCATGATCGCTGCGGTCGTCGGCGCCGTGACGCTGGTAGTGCAACT
CATAACCATTCCGAGGCTGCCTCCGATTGGAGTGGCCAGCTTCCGCAGTCTGCTGGATGTGGCCAAGAATCCGATGATCA
AAGTCGCGATGTTGGTCGTCCTGCTGGTCGCTTCCGGGCACTTCGCCGGCTTCACCTATATCCGCGCCTTCCTTGAGAAG
TTTCCCGCGCTTGATATCGAGACGATCTCGCTGGTGTTGCTCGTCTTTGGCATCGGCGGCTTCTTCGGCAATTTGGCCGG
CGGATTCCTGGCCGAGCACAGCCTCAAGGCAGCCGCGGCCCTGCCTCCCCTGTTCATAGCGATGGCCACTGTCTCGCTGC
TGACGCTGGGAGCATCGGCTTGGGCCACGGCGATTGCGGTTACAGTATGGGGCTTTGCCTTCGGCGCGGTGCCGGTGGGA
CTGCAGACATGGATGGTGCTGCACGCCGCTCCCGAGCAGGCCGAAAGCGCCGGTGGGCTGATGGCCGCAACGTTCCAGGT
AGCCATCGCAGCGGGCGCGATTTTCGGCGGACTACTGATGGACAATGCCGGCGTCGCCAGTTCCCTTGCCTACAGCGCCG
TTGCCTCGTTCCTCGGCGCCCTGACAGTGTTCTTGCTCGGCCCGAAGCGCGAACCTCAGACCACCTGA

Upstream 100 bases:

>100_bases
TTTCACCGTCGCGAGCGCGTCCTCCCAAGGTGGCGACGCGCGGTCCCCCCCGAAGCTGTCCGCCATCGGATCAAGGCGAC
AATCGCCGACGAATCGCAAG

Downstream 100 bases:

>100_bases
CAGAGAGGCCATCAGTGCAGCGCGGGAAAGATGCAATCATCAGCCGCGCGGTGCGATCCTACGCACGATTTTTGGCGGTT
GCAGGCCTCGTCTCCGGGTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTRFPTTTSSSGPRKGPSICAPHRHKISRSDEHPLVRAMPQGMALLALIRVNAATRVCSTSKLETLLMTESATGFMDGVL
HDPKQPDQRSGPAWGAVISLALGTFGLVTAEFLPASVLTPLAHDLSITEGAVGQALTTTAIVGAISAPTMAVITRPLDRR
IVIWAMTLLLILSNVLSAVAGSLPVLLAARVVLGIALGGFWSISAALAMRLVPSHLLRRAMSIILTGVTCATVCAAPIGA
YVGDIWGWRTAFMIAAVVGAVTLVVQLITIPRLPPIGVASFRSLLDVAKNPMIKVAMLVVLLVASGHFAGFTYIRAFLEK
FPALDIETISLVLLVFGIGGFFGNLAGGFLAEHSLKAAAALPPLFIAMATVSLLTLGASAWATAIAVTVWGFAFGAVPVG
LQTWMVLHAAPEQAESAGGLMAATFQVAIAAGAIFGGLLMDNAGVASSLAYSAVASFLGALTVFLLGPKREPQTT

Sequences:

>Translated_475_residues
MTRFPTTTSSSGPRKGPSICAPHRHKISRSDEHPLVRAMPQGMALLALIRVNAATRVCSTSKLETLLMTESATGFMDGVL
HDPKQPDQRSGPAWGAVISLALGTFGLVTAEFLPASVLTPLAHDLSITEGAVGQALTTTAIVGAISAPTMAVITRPLDRR
IVIWAMTLLLILSNVLSAVAGSLPVLLAARVVLGIALGGFWSISAALAMRLVPSHLLRRAMSIILTGVTCATVCAAPIGA
YVGDIWGWRTAFMIAAVVGAVTLVVQLITIPRLPPIGVASFRSLLDVAKNPMIKVAMLVVLLVASGHFAGFTYIRAFLEK
FPALDIETISLVLLVFGIGGFFGNLAGGFLAEHSLKAAAALPPLFIAMATVSLLTLGASAWATAIAVTVWGFAFGAVPVG
LQTWMVLHAAPEQAESAGGLMAATFQVAIAAGAIFGGLLMDNAGVASSLAYSAVASFLGALTVFLLGPKREPQTT
>Mature_474_residues
TRFPTTTSSSGPRKGPSICAPHRHKISRSDEHPLVRAMPQGMALLALIRVNAATRVCSTSKLETLLMTESATGFMDGVLH
DPKQPDQRSGPAWGAVISLALGTFGLVTAEFLPASVLTPLAHDLSITEGAVGQALTTTAIVGAISAPTMAVITRPLDRRI
VIWAMTLLLILSNVLSAVAGSLPVLLAARVVLGIALGGFWSISAALAMRLVPSHLLRRAMSIILTGVTCATVCAAPIGAY
VGDIWGWRTAFMIAAVVGAVTLVVQLITIPRLPPIGVASFRSLLDVAKNPMIKVAMLVVLLVASGHFAGFTYIRAFLEKF
PALDIETISLVLLVFGIGGFFGNLAGGFLAEHSLKAAAALPPLFIAMATVSLLTLGASAWATAIAVTVWGFAFGAVPVGL
QTWMVLHAAPEQAESAGGLMAATFQVAIAAGAIFGGLLMDNAGVASSLAYSAVASFLGALTVFLLGPKREPQTT

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=389, Percent_Identity=37.0179948586118, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1787808, Length=345, Percent_Identity=28.695652173913, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787947, Length=322, Percent_Identity=26.3975155279503, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1786595, Length=384, Percent_Identity=23.4375, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49203; Mature: 49072

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFPTTTSSSGPRKGPSICAPHRHKISRSDEHPLVRAMPQGMALLALIRVNAATRVCST
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
SKLETLLMTESATGFMDGVLHDPKQPDQRSGPAWGAVISLALGTFGLVTAEFLPASVLTP
HHHHHHHEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAHDLSITEGAVGQALTTTAIVGAISAPTMAVITRPLDRRIVIWAMTLLLILSNVLSAVA
HHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
GSLPVLLAARVVLGIALGGFWSISAALAMRLVPSHLLRRAMSIILTGVTCATVCAAPIGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVGDIWGWRTAFMIAAVVGAVTLVVQLITIPRLPPIGVASFRSLLDVAKNPMIKVAMLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
LLVASGHFAGFTYIRAFLEKFPALDIETISLVLLVFGIGGFFGNLAGGFLAEHSLKAAAA
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPPLFIAMATVSLLTLGASAWATAIAVTVWGFAFGAVPVGLQTWMVLHAAPEQAESAGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCH
MAATFQVAIAAGAIFGGLLMDNAGVASSLAYSAVASFLGALTVFLLGPKREPQTT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TRFPTTTSSSGPRKGPSICAPHRHKISRSDEHPLVRAMPQGMALLALIRVNAATRVCST
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
SKLETLLMTESATGFMDGVLHDPKQPDQRSGPAWGAVISLALGTFGLVTAEFLPASVLTP
HHHHHHHEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAHDLSITEGAVGQALTTTAIVGAISAPTMAVITRPLDRRIVIWAMTLLLILSNVLSAVA
HHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
GSLPVLLAARVVLGIALGGFWSISAALAMRLVPSHLLRRAMSIILTGVTCATVCAAPIGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVGDIWGWRTAFMIAAVVGAVTLVVQLITIPRLPPIGVASFRSLLDVAKNPMIKVAMLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
LLVASGHFAGFTYIRAFLEKFPALDIETISLVLLVFGIGGFFGNLAGGFLAEHSLKAAAA
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPPLFIAMATVSLLTLGASAWATAIAVTVWGFAFGAVPVGLQTWMVLHAAPEQAESAGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCH
MAATFQVAIAAGAIFGGLLMDNAGVASSLAYSAVASFLGALTVFLLGPKREPQTT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA