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The map label for this gene is rhtB [H]

Identifier: 13475161

GI number: 13475161

Start: 4997763

End: 4998413

Strand: Direct

Name: rhtB [H]

Synonym: mlr6177

Alternate gene names: 13475161

Gene position: 4997763-4998413 (Clockwise)

Preceding gene: 13475160

Following gene: 13475162

Centisome position: 71.03

GC content: 65.9

Gene sequence:

>651_bases
ATGCCAGAACTGACCCCACTCGCCCTGTACCTCGCCGCGGCCTCCGTGCTCGCCATCACCCCCGGTCCGGGCATCTTCTA
CGTTGCCGCGCGCACGCTCGCCGGTGGTCGTCGGGAAGGTATCGCTTCCAGCTTCGGCACGGGGTTGGGGGGCATGATCC
ACGTCCTCGCCGCAAGCTTAGGTGTTTCCGCGATCGTGCTCGAGAGCTCGGAACTGTTCTCCGCATTAAAGCTGTTCGGC
GCGGTCTACCTCGTCTGGCTCGGCTTTCGCACCTTTCAAGCCGCCCGCCTCAAGGGAGCGACTGGCGGCGATGACGGCGC
CGCAGTGGGTCCAGTCGGACCCCGGCGGGCGTTTCGTGAAGGGGTACTGGTCGAGGCGCTCAACCCGAAGACGGCAGTCT
TTTTCCTCGCCTTCATTCCGCAGTTCGTGAATCCGAGCGCAGGGCTCGTAGCTCTGCAATTCGTGGTGCTTGGCTCCGTG
TCCATTGCGCTCAACACGCTCGCCGACATCGTGGTTGCCGTCGTGGCCAGCGGCATTCAAGACGGCGCGGCAGCGCGTCC
CGGGTTGATCCGCCGCCTGAGGGAGGCGTCAGGTGGCGCGATGATCGCGCTGGGCGTCGGCCTCGCTCTGGCGAAACACC
CTGCTGCCTGA

Upstream 100 bases:

>100_bases
TGGCGTGGCGGCGGGCCTTGCGGCGAGCGAGCGGGCATCTGACCGCGACGTCGCCGCTCTCATCCCAAAATAATCATGAA
CTTCGTCGGAATCGACCATC

Downstream 100 bases:

>100_bases
CTTTGGCGGGTACCTGGCGGAAGCAGTTGGATGGCTGCGAGCGAGTTCGGTCATTGCACCTATTTAACCATGTCTCAGGA
GGCGCGGCACCGCCGTTTGG

Product: hypothetical protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MPELTPLALYLAAASVLAITPGPGIFYVAARTLAGGRREGIASSFGTGLGGMIHVLAASLGVSAIVLESSELFSALKLFG
AVYLVWLGFRTFQAARLKGATGGDDGAAVGPVGPRRAFREGVLVEALNPKTAVFFLAFIPQFVNPSAGLVALQFVVLGSV
SIALNTLADIVVAVVASGIQDGAAARPGLIRRLREASGGAMIALGVGLALAKHPAA

Sequences:

>Translated_216_residues
MPELTPLALYLAAASVLAITPGPGIFYVAARTLAGGRREGIASSFGTGLGGMIHVLAASLGVSAIVLESSELFSALKLFG
AVYLVWLGFRTFQAARLKGATGGDDGAAVGPVGPRRAFREGVLVEALNPKTAVFFLAFIPQFVNPSAGLVALQFVVLGSV
SIALNTLADIVVAVVASGIQDGAAARPGLIRRLREASGGAMIALGVGLALAKHPAA
>Mature_215_residues
PELTPLALYLAAASVLAITPGPGIFYVAARTLAGGRREGIASSFGTGLGGMIHVLAASLGVSAIVLESSELFSALKLFGA
VYLVWLGFRTFQAARLKGATGGDDGAAVGPVGPRRAFREGVLVEALNPKTAVFFLAFIPQFVNPSAGLVALQFVVLGSVS
IALNTLADIVVAVVASGIQDGAAARPGLIRRLREASGGAMIALGVGLALAKHPAA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=205, Percent_Identity=30.7317073170732, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788099, Length=149, Percent_Identity=24.8322147651007, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21820; Mature: 21689

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELTPLALYLAAASVLAITPGPGIFYVAARTLAGGRREGIASSFGTGLGGMIHVLAASL
CCCCHHHHHHHHHHHHEEECCCCCEEHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
GVSAIVLESSELFSALKLFGAVYLVWLGFRTFQAARLKGATGGDDGAAVGPVGPRRAFRE
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHC
GVLVEALNPKTAVFFLAFIPQFVNPSAGLVALQFVVLGSVSIALNTLADIVVAVVASGIQ
CCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGAAARPGLIRRLREASGGAMIALGVGLALAKHPAA
CCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHCCCCC
>Mature Secondary Structure 
PELTPLALYLAAASVLAITPGPGIFYVAARTLAGGRREGIASSFGTGLGGMIHVLAASL
CCCHHHHHHHHHHHHEEECCCCCEEHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
GVSAIVLESSELFSALKLFGAVYLVWLGFRTFQAARLKGATGGDDGAAVGPVGPRRAFRE
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHC
GVLVEALNPKTAVFFLAFIPQFVNPSAGLVALQFVVLGSVSIALNTLADIVVAVVASGIQ
CCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGAAARPGLIRRLREASGGAMIALGVGLALAKHPAA
CCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]