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The map label for this gene is metK

Identifier: 13475107

GI number: 13475107

Start: 4948938

End: 4950113

Strand: Direct

Name: metK

Synonym: mlr6115

Alternate gene names: 13475107

Gene position: 4948938-4950113 (Clockwise)

Preceding gene: 13475106

Following gene: 13475108

Centisome position: 70.34

GC content: 59.1

Gene sequence:

>1176_bases
ATGACCAGGCAGTTCGTGTTCTCTTCCGAATCCGTCGGCGCGGGACACCCCGATAAGATGGCAGATAACATCTCGGATGC
CATCCTCGATGCCATCCTCCGCACCGACCCGAAGGCGCGCGTCGCCTGTGAAACGTTGGTGAAGACAGGGATGGTCGTTC
TGGCTGGAGAGATCACCAGCCACGCGGTGATCGATTATACTCAAGTTGCCCGCGATACGATCCTCGATATTGGCTACGAC
GACGATGGCATCGGCTTTGATGGTCGACGTTGCGCCGTCGTTCTGGCTCTCACCGAGCAGTCGCCCGACATAAGCCAGGG
CGTTGATGAGGGACGAGGGCAGGATCTCGAGCAGGGGGCGGGGGATCAGGGCCTCATGTTTGGATTCGCATGCAACGAGA
CGGATACATTGATGCCCTTGCCGATCCAACTCGCTCATCATTTGACGAAAAGACAGGCTGAGGTCCGGAAAACGGGACAG
CTTGGCTGGCTGCGTCCGGATGTGAAATCTCAAGTGTCCGTACGCTACGAGGGTTTGCGCCCGGTGGCGCTGGATACCAT
TGTCCTGTCGACGCAGCATGATGAGGCGGTCTCACAAGCCACGGTCCGGGAAGGGGTCATTGAGGAGATCATAAAACCGG
TCCTGCCGACCCACCTGGATACGACAGGGATCAGATTTCTGGTCAACCCAACAGGGCGGTTCGTGGTCGGTGGGCCTGCC
GGCGACTGCGGCCTCACTGGACGCAAGATCATCGTCGATTCCTACGGTGGAACGGGGCGTCACGGCGGCGGCGCCTTTTC
GGGCAAGGACCCATCCAAGGTTGACCGCTCGGCCGCCTATGCCGCCCGGTATGTCGCGAAGAATATCGTTGCCAGCGGCC
TTGCCGAGGTCTGCGAGGTTCAACTCGCCTACGCGATCGGCGTGGCCAGTCCGGTTTCTGTCATGGTCAACACCTTCGGA
ACTGCAAGGATCGAGGAAAAAAGGATCGAGTGCCTTGTTCTTGAGCTTTTCGATCTCCGACCGAAAGGCATCATCAAGAT
GCTTGATCTCGTACGCCCGATATACCGCAACACGGCGACATACGGACACTTCGGCCGTGAAGAGCCTGAGTTCACCTGGG
AGAAGACGGACAGAGCCGACGACTTGCTGCGTGAAGCAGGACCGGCAGCTGCGTGA

Upstream 100 bases:

>100_bases
CTTCTGCACTGCGTTCGACGTCCCCGGCATCAGCGACCGGTAAGACCGCCCCAGTCCAGAAACTCCCATCCAACTCCAAC
ACTTGAAGCGAGGACATTCC

Downstream 100 bases:

>100_bases
GGGCCGCTGGATCAAGCGCATGCGGCAACCCTTTTCCTCGCTAGACCCCCGCCCGGCCGGCATAGCAGAAAGCCGCTGGG
TAGTAGGCGGAGACATTGAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTRQFVFSSESVGAGHPDKMADNISDAILDAILRTDPKARVACETLVKTGMVVLAGEITSHAVIDYTQVARDTILDIGYD
DDGIGFDGRRCAVVLALTEQSPDISQGVDEGRGQDLEQGAGDQGLMFGFACNETDTLMPLPIQLAHHLTKRQAEVRKTGQ
LGWLRPDVKSQVSVRYEGLRPVALDTIVLSTQHDEAVSQATVREGVIEEIIKPVLPTHLDTTGIRFLVNPTGRFVVGGPA
GDCGLTGRKIIVDSYGGTGRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVASGLAEVCEVQLAYAIGVASPVSVMVNTFG
TARIEEKRIECLVLELFDLRPKGIIKMLDLVRPIYRNTATYGHFGREEPEFTWEKTDRADDLLREAGPAAA

Sequences:

>Translated_391_residues
MTRQFVFSSESVGAGHPDKMADNISDAILDAILRTDPKARVACETLVKTGMVVLAGEITSHAVIDYTQVARDTILDIGYD
DDGIGFDGRRCAVVLALTEQSPDISQGVDEGRGQDLEQGAGDQGLMFGFACNETDTLMPLPIQLAHHLTKRQAEVRKTGQ
LGWLRPDVKSQVSVRYEGLRPVALDTIVLSTQHDEAVSQATVREGVIEEIIKPVLPTHLDTTGIRFLVNPTGRFVVGGPA
GDCGLTGRKIIVDSYGGTGRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVASGLAEVCEVQLAYAIGVASPVSVMVNTFG
TARIEEKRIECLVLELFDLRPKGIIKMLDLVRPIYRNTATYGHFGREEPEFTWEKTDRADDLLREAGPAAA
>Mature_390_residues
TRQFVFSSESVGAGHPDKMADNISDAILDAILRTDPKARVACETLVKTGMVVLAGEITSHAVIDYTQVARDTILDIGYDD
DGIGFDGRRCAVVLALTEQSPDISQGVDEGRGQDLEQGAGDQGLMFGFACNETDTLMPLPIQLAHHLTKRQAEVRKTGQL
GWLRPDVKSQVSVRYEGLRPVALDTIVLSTQHDEAVSQATVREGVIEEIIKPVLPTHLDTTGIRFLVNPTGRFVVGGPAG
DCGLTGRKIIVDSYGGTGRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVASGLAEVCEVQLAYAIGVASPVSVMVNTFGT
ARIEEKRIECLVLELFDLRPKGIIKMLDLVRPIYRNTATYGHFGREEPEFTWEKTDRADDLLREAGPAAA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=55.3475935828877, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=55.7333333333333, Blast_Score=394, Evalue=1e-109,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=64.490861618799, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17538498, Length=395, Percent_Identity=53.6708860759494, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=54.9071618037135, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551082, Length=380, Percent_Identity=53.4210526315789, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25145633, Length=396, Percent_Identity=52.7777777777778, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565911, Length=347, Percent_Identity=51.8731988472622, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=48.5411140583554, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=348, Percent_Identity=50.8620689655172, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=52.6490066225166, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=59.3023255813954, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320710, Length=382, Percent_Identity=52.6178010471204, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323209, Length=380, Percent_Identity=53.1578947368421, Blast_Score=391, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45552159, Length=375, Percent_Identity=54.1333333333333, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580529, Length=375, Percent_Identity=54.1333333333333, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580537, Length=375, Percent_Identity=54.1333333333333, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580533, Length=375, Percent_Identity=54.1333333333333, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580535, Length=375, Percent_Identity=54.1333333333333, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580531, Length=375, Percent_Identity=54.1333333333333, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580527, Length=375, Percent_Identity=52.8, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580525, Length=375, Percent_Identity=52.8, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580541, Length=362, Percent_Identity=51.9337016574586, Blast_Score=346, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=57.9831932773109, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_RHILO (Q98A80)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106671.1
- ProteinModelPortal:   Q98A80
- SMR:   Q98A80
- GeneID:   1229326
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr6115
- NMPDR:   fig|266835.1.peg.4772
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK12459
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42195; Mature: 42064

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQFVFSSESVGAGHPDKMADNISDAILDAILRTDPKARVACETLVKTGMVVLAGEITS
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCC
HAVIDYTQVARDTILDIGYDDDGIGFDGRRCAVVLALTEQSPDISQGVDEGRGQDLEQGA
CHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEEEEEECCCCCHHHCCCCCCCCCHHHCC
GDQGLMFGFACNETDTLMPLPIQLAHHLTKRQAEVRKTGQLGWLRPDVKSQVSVRYEGLR
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEECCCC
PVALDTIVLSTQHDEAVSQATVREGVIEEIIKPVLPTHLDTTGIRFLVNPTGRFVVGGPA
CEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCC
GDCGLTGRKIIVDSYGGTGRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVASGLAEVCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLAYAIGVASPVSVMVNTFGTARIEEKRIECLVLELFDLRPKGIIKMLDLVRPIYRNTAT
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHEEHHHCCCCHHHHHHHHHHHHHHHCCCC
YGHFGREEPEFTWEKTDRADDLLREAGPAAA
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
TRQFVFSSESVGAGHPDKMADNISDAILDAILRTDPKARVACETLVKTGMVVLAGEITS
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCC
HAVIDYTQVARDTILDIGYDDDGIGFDGRRCAVVLALTEQSPDISQGVDEGRGQDLEQGA
CHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEEEEEECCCCCHHHCCCCCCCCCHHHCC
GDQGLMFGFACNETDTLMPLPIQLAHHLTKRQAEVRKTGQLGWLRPDVKSQVSVRYEGLR
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEECCCC
PVALDTIVLSTQHDEAVSQATVREGVIEEIIKPVLPTHLDTTGIRFLVNPTGRFVVGGPA
CEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCC
GDCGLTGRKIIVDSYGGTGRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVASGLAEVCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLAYAIGVASPVSVMVNTFGTARIEEKRIECLVLELFDLRPKGIIKMLDLVRPIYRNTAT
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHEEHHHCCCCHHHHHHHHHHHHHHHCCCC
YGHFGREEPEFTWEKTDRADDLLREAGPAAA
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968