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The map label for this gene is ptrB [C]

Identifier: 13475081

GI number: 13475081

Start: 4924742

End: 4926859

Strand: Direct

Name: ptrB [C]

Synonym: mlr6087

Alternate gene names: 13475081

Gene position: 4924742-4926859 (Clockwise)

Preceding gene: 13475080

Following gene: 13475087

Centisome position: 69.99

GC content: 58.83

Gene sequence:

>2118_bases
ATGCGGAACATACGCCTGCAGCCACCGCTTCCTCGTGCCGAGTCTCGGATCAGAGTTTTTCACGACGACGTCACGATCGA
TTCGTATCGTTGGTTTCGCGACCGAGAGAATCCCGATGTCGGCGCGTATCTGGAAGCCGAGAACAGCTACACAGAGCAAG
CGACAGCTCACCTTTCAGCGCTTAAGGCGGAGATCATCGCCGAGATCGAAGGGCGTCAGCCTCGCGAGTACACTTCACCA
GCGTTGCAAGTTGGGCCTTTCGAGTACTTCCAAAGGCGCGAGCCGAACTTGGCTCATCCGGCCTGGTGGCGTCGCCCAGT
AACTGGCGGCTCGGAGGAACAAGTTCTTGACCCGAACGCGATTCCCGGTGCGGAGGTCTTCTACTCACTCGGTGTGTTCG
AACCGTGTGACGACGGGCGTTACGTGGCCTTCAGCTTCGATGTCATTGGGAACGAGAATTACGAGCTTAGGGTTCGGGAC
ATGAAGACCGGCCAAGAGGTCTGGCAGGGATCGAAGAAGGTTGCGATGGTGGCTTGGGCCGCGGATGGTAACACTTTGTT
TTTCACGAGCGAGCGACCCAGCCGACAACAATGTCAGGAGCTTGTCCGCCTGAATATAGCAACCGGGATTTCTGAGGTAG
TATTTGAGGAAAGCAATGAGCAATTAAAAGTTCAGGTTCGCCGTTCTGATAGTGGCGCCTGGTTGTTCCTCGACGTGACT
GCTTTCTCGGGCCATTCCTCTTTTCCCGCTCATTTCGGTGCTGAAGAGGTGTGGTGTCTTCCTGCCGACGAGCCGGGAGG
CAACTGGCGTCGGATTGTGAAGCGGGGAAACGGACACGAGATTTATGCAGAGCATTGGGGCGATAGTTTCCTGTTTCGAG
TGAACGACGCTGGACCGTATTGCCGGCTCGTGTGTGCGCCGATAGATGACCCGTCACCTTCGTCTTGGGAGGAGGTAATC
CCCCATCGTGAAGGCGCGACACTCGAAGAAGTGCACATCTTCGAGAAGCACTTGGTTGTGCTGGAACGAGAAGGCCTGCG
TCCTCGCCTGGTCGCACGTGACAGAAGCGGGCGTGTTGGAACCACGATCGATCCCGAGGAGTCGAGCTGCACCCTTAAGG
TCGGCTCGTCAGCAGGGGGGACGCATTCGGTGCCACGGCATTTGTTCCGAAGCTCCAAGTTGGTTTACTCAATTAGCTCG
TTTGTGACACCCGACACCTTCGTCGAGCATGATCTCGCCGACGACCGTTCGGTGACCTTGTGCCGGGCCCACATACCGGG
GTTTGACCCTGCTTTATACGTCTCGCAGGTTGTGATGGTGGAGGCGGACGACGGGGTTGAGATACCGATTTCGCTTGTTA
CACGTCGCGACCGGGCTAACCCTGGGCCGGTCGTACTCCGCGTCTACGGGTGTTACGGGTCACCAAGATCTCCGTCGTTC
AAGGATTGGCCTTCATTCATGACTGGTCGCTTAAGCCTGCTCGACCGAGGGGTTGCTTTCGGTTGGGCTCACATACGTGG
TGGCGGTGAACTCGGTCGCCCCTCGCACGAGGCAGCTATGGCTGAACGAAAACGCACTACCCACACAGATCTGATCGCCG
CTGCCGAGGGACTTGTTAAGCAGGGCTTCGCCACCCGCGACGGGATCGCCATCGAGGGAGCGAGCGCTGGCGGCGGCGTT
GTGCTTGCTACGGCCGCCTTGCGGCCGGACTTGTTCCGCGCCGTGATTGCCGAGGTTCCGCTCGCCGACATCCTGGATAC
TGAAATGGACGCCGCAATGCCGTGGGGTCTATGGGAGAGGGCGGAGTATGGAAATCCCCACATCGCCAACGAATATCGGT
ACCTGCTCAGCTACGACCCCTACTACAACCTCAGCGCCGATCGCCCACTTCCGCCGACATATGTCGACGCCGCGCTCGAT
GACGGCCAGGTGCTGTATTATCAGCCCGCTCGTTATGTGGCGCAGCGTCGATCCTGTGCTGCCGATCGCGATCCCAAGCT
GGTTTTCCGCACGCGGATGGTCGGCGGCCACAGCGGCGCTTCACATGGGCCTGGGGTAGCTGAGGAAAGAGCATTTCGCA
TGGCCTGGGCGCTCGACCAGCTCCGCACGATCAAATAG

Upstream 100 bases:

>100_bases
AGAGATGAGGGGCTTCCCACGCTGTCTCTCTGGAGGGAATACTAATGCACAAACTATATTGTTTGCTGATTGCTGCGACT
TTTCAGACGAGGGTCTTCTG

Downstream 100 bases:

>100_bases
CTCAGCTCATTGTCATCTATCTCACGTGGTGGCGGTCCCGGCCGAGAGATCACCACTCGCTCCCGGAACTTGCTACTTGA
GCTGAAGGCCGACGGCCACG

Product: dipeptidyl aminopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MRNIRLQPPLPRAESRIRVFHDDVTIDSYRWFRDRENPDVGAYLEAENSYTEQATAHLSALKAEIIAEIEGRQPREYTSP
ALQVGPFEYFQRREPNLAHPAWWRRPVTGGSEEQVLDPNAIPGAEVFYSLGVFEPCDDGRYVAFSFDVIGNENYELRVRD
MKTGQEVWQGSKKVAMVAWAADGNTLFFTSERPSRQQCQELVRLNIATGISEVVFEESNEQLKVQVRRSDSGAWLFLDVT
AFSGHSSFPAHFGAEEVWCLPADEPGGNWRRIVKRGNGHEIYAEHWGDSFLFRVNDAGPYCRLVCAPIDDPSPSSWEEVI
PHREGATLEEVHIFEKHLVVLEREGLRPRLVARDRSGRVGTTIDPEESSCTLKVGSSAGGTHSVPRHLFRSSKLVYSISS
FVTPDTFVEHDLADDRSVTLCRAHIPGFDPALYVSQVVMVEADDGVEIPISLVTRRDRANPGPVVLRVYGCYGSPRSPSF
KDWPSFMTGRLSLLDRGVAFGWAHIRGGGELGRPSHEAAMAERKRTTHTDLIAAAEGLVKQGFATRDGIAIEGASAGGGV
VLATAALRPDLFRAVIAEVPLADILDTEMDAAMPWGLWERAEYGNPHIANEYRYLLSYDPYYNLSADRPLPPTYVDAALD
DGQVLYYQPARYVAQRRSCAADRDPKLVFRTRMVGGHSGASHGPGVAEERAFRMAWALDQLRTIK

Sequences:

>Translated_705_residues
MRNIRLQPPLPRAESRIRVFHDDVTIDSYRWFRDRENPDVGAYLEAENSYTEQATAHLSALKAEIIAEIEGRQPREYTSP
ALQVGPFEYFQRREPNLAHPAWWRRPVTGGSEEQVLDPNAIPGAEVFYSLGVFEPCDDGRYVAFSFDVIGNENYELRVRD
MKTGQEVWQGSKKVAMVAWAADGNTLFFTSERPSRQQCQELVRLNIATGISEVVFEESNEQLKVQVRRSDSGAWLFLDVT
AFSGHSSFPAHFGAEEVWCLPADEPGGNWRRIVKRGNGHEIYAEHWGDSFLFRVNDAGPYCRLVCAPIDDPSPSSWEEVI
PHREGATLEEVHIFEKHLVVLEREGLRPRLVARDRSGRVGTTIDPEESSCTLKVGSSAGGTHSVPRHLFRSSKLVYSISS
FVTPDTFVEHDLADDRSVTLCRAHIPGFDPALYVSQVVMVEADDGVEIPISLVTRRDRANPGPVVLRVYGCYGSPRSPSF
KDWPSFMTGRLSLLDRGVAFGWAHIRGGGELGRPSHEAAMAERKRTTHTDLIAAAEGLVKQGFATRDGIAIEGASAGGGV
VLATAALRPDLFRAVIAEVPLADILDTEMDAAMPWGLWERAEYGNPHIANEYRYLLSYDPYYNLSADRPLPPTYVDAALD
DGQVLYYQPARYVAQRRSCAADRDPKLVFRTRMVGGHSGASHGPGVAEERAFRMAWALDQLRTIK
>Mature_705_residues
MRNIRLQPPLPRAESRIRVFHDDVTIDSYRWFRDRENPDVGAYLEAENSYTEQATAHLSALKAEIIAEIEGRQPREYTSP
ALQVGPFEYFQRREPNLAHPAWWRRPVTGGSEEQVLDPNAIPGAEVFYSLGVFEPCDDGRYVAFSFDVIGNENYELRVRD
MKTGQEVWQGSKKVAMVAWAADGNTLFFTSERPSRQQCQELVRLNIATGISEVVFEESNEQLKVQVRRSDSGAWLFLDVT
AFSGHSSFPAHFGAEEVWCLPADEPGGNWRRIVKRGNGHEIYAEHWGDSFLFRVNDAGPYCRLVCAPIDDPSPSSWEEVI
PHREGATLEEVHIFEKHLVVLEREGLRPRLVARDRSGRVGTTIDPEESSCTLKVGSSAGGTHSVPRHLFRSSKLVYSISS
FVTPDTFVEHDLADDRSVTLCRAHIPGFDPALYVSQVVMVEADDGVEIPISLVTRRDRANPGPVVLRVYGCYGSPRSPSF
KDWPSFMTGRLSLLDRGVAFGWAHIRGGGELGRPSHEAAMAERKRTTHTDLIAAAEGLVKQGFATRDGIAIEGASAGGGV
VLATAALRPDLFRAVIAEVPLADILDTEMDAAMPWGLWERAEYGNPHIANEYRYLLSYDPYYNLSADRPLPPTYVDAALD
DGQVLYYQPARYVAQRRSCAADRDPKLVFRTRMVGGHSGASHGPGVAEERAFRMAWALDQLRTIK

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1770

COG function: function code E; Protease II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9A family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=737, Percent_Identity=22.9308005427408, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI284172420, Length=474, Percent_Identity=25.3164556962025, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI284172413, Length=474, Percent_Identity=25.3164556962025, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI70778815, Length=474, Percent_Identity=25.3164556962025, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI284172438, Length=474, Percent_Identity=25.3164556962025, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI284172431, Length=474, Percent_Identity=25.3164556962025, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI108860686, Length=221, Percent_Identity=32.1266968325792, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI108860692, Length=204, Percent_Identity=31.8627450980392, Blast_Score=90, Evalue=6e-18,
Organism=Escherichia coli, GI1788150, Length=710, Percent_Identity=31.5492957746479, Blast_Score=283, Evalue=2e-77,
Organism=Drosophila melanogaster, GI221510989, Length=737, Percent_Identity=24.6947082767978, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24583414, Length=714, Percent_Identity=23.249299719888, Blast_Score=162, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 78675; Mature: 78675

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIRLQPPLPRAESRIRVFHDDVTIDSYRWFRDRENPDVGAYLEAENSYTEQATAHLSA
CCCCCCCCCCCCCCCCEEEEECCEEECCCHHHCCCCCCCCCEEEECCCCCHHHHHHHHHH
LKAEIIAEIEGRQPREYTSPALQVGPFEYFQRREPNLAHPAWWRRPVTGGSEEQVLDPNA
HHHHHHHHCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCHHHCCCCCCCCCCCEECCCC
IPGAEVFYSLGVFEPCDDGRYVAFSFDVIGNENYELRVRDMKTGQEVWQGSKKVAMVAWA
CCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHCCCCEEEEEEEE
ADGNTLFFTSERPSRQQCQELVRLNIATGISEVVFEESNEQLKVQVRRSDSGAWLFLDVT
CCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEEECCCCCEEEEEEE
AFSGHSSFPAHFGAEEVWCLPADEPGGNWRRIVKRGNGHEIYAEHWGDSFLFRVNDAGPY
EECCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCEEEEEECCCCCE
CRLVCAPIDDPSPSSWEEVIPHREGATLEEVHIFEKHLVVLEREGLRPRLVARDRSGRVG
EEEEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCC
TTIDPEESSCTLKVGSSAGGTHSVPRHLFRSSKLVYSISSFVTPDTFVEHDLADDRSVTL
CEECCCCCCEEEEECCCCCCCCHHHHHHHHCCHHEEEHHHHCCCCHHHHHCCCCCCCEEE
CRAHIPGFDPALYVSQVVMVEADDGVEIPISLVTRRDRANPGPVVLRVYGCYGSPRSPSF
EEECCCCCCHHHHEEEEEEEECCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
KDWPSFMTGRLSLLDRGVAFGWAHIRGGGELGRPSHEAAMAERKRTTHTDLIAAAEGLVK
CCCHHHHHHHHHHHHCCHHEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
QGFATRDGIAIEGASAGGGVVLATAALRPDLFRAVIAEVPLADILDTEMDAAMPWGLWER
CCCCCCCCEEEECCCCCCCEEEEEEHHCHHHHHHHHHHCCHHHHHCCHHHHCCCCCCHHH
AEYGNPHIANEYRYLLSYDPYYNLSADRPLPPTYVDAALDDGQVLYYQPARYVAQRRSCA
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCHHHHHHHHHHC
ADRDPKLVFRTRMVGGHSGASHGPGVAEERAFRMAWALDQLRTIK
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRNIRLQPPLPRAESRIRVFHDDVTIDSYRWFRDRENPDVGAYLEAENSYTEQATAHLSA
CCCCCCCCCCCCCCCCEEEEECCEEECCCHHHCCCCCCCCCEEEECCCCCHHHHHHHHHH
LKAEIIAEIEGRQPREYTSPALQVGPFEYFQRREPNLAHPAWWRRPVTGGSEEQVLDPNA
HHHHHHHHCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCHHHCCCCCCCCCCCEECCCC
IPGAEVFYSLGVFEPCDDGRYVAFSFDVIGNENYELRVRDMKTGQEVWQGSKKVAMVAWA
CCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHCCCCEEEEEEEE
ADGNTLFFTSERPSRQQCQELVRLNIATGISEVVFEESNEQLKVQVRRSDSGAWLFLDVT
CCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEEECCCCCEEEEEEE
AFSGHSSFPAHFGAEEVWCLPADEPGGNWRRIVKRGNGHEIYAEHWGDSFLFRVNDAGPY
EECCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCEEEEEECCCCCE
CRLVCAPIDDPSPSSWEEVIPHREGATLEEVHIFEKHLVVLEREGLRPRLVARDRSGRVG
EEEEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCC
TTIDPEESSCTLKVGSSAGGTHSVPRHLFRSSKLVYSISSFVTPDTFVEHDLADDRSVTL
CEECCCCCCEEEEECCCCCCCCHHHHHHHHCCHHEEEHHHHCCCCHHHHHCCCCCCCEEE
CRAHIPGFDPALYVSQVVMVEADDGVEIPISLVTRRDRANPGPVVLRVYGCYGSPRSPSF
EEECCCCCCHHHHEEEEEEEECCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
KDWPSFMTGRLSLLDRGVAFGWAHIRGGGELGRPSHEAAMAERKRTTHTDLIAAAEGLVK
CCCHHHHHHHHHHHHCCHHEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
QGFATRDGIAIEGASAGGGVVLATAALRPDLFRAVIAEVPLADILDTEMDAAMPWGLWER
CCCCCCCCEEEECCCCCCCEEEEEEHHCHHHHHHHHHHCCHHHHHCCHHHHCCCCCCHHH
AEYGNPHIANEYRYLLSYDPYYNLSADRPLPPTYVDAALDDGQVLYYQPARYVAQRRSCA
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCHHHHHHHHHHC
ADRDPKLVFRTRMVGGHSGASHGPGVAEERAFRMAWALDQLRTIK
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]