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The map label for this gene is citE [H]

Identifier: 13475020

GI number: 13475020

Start: 4857786

End: 4858655

Strand: Reverse

Name: citE [H]

Synonym: mll6008

Alternate gene names: 13475020

Gene position: 4858655-4857786 (Counterclockwise)

Preceding gene: 13475023

Following gene: 13475019

Centisome position: 69.05

GC content: 62.76

Gene sequence:

>870_bases
ATGCGCTCACTGCTTTTCGTTCCGGGCGACTCGGCCCGCAAGCAGGACAAGGCGATGGAGACGGGCGCCGACCTCCTGAT
CCTCGACCTCGAAGATTCGGTCGCGCTGTCCGCAAAGGAAGACGCGCGCAAGCTCACCCGCGCCTTCGTCGAACGCAACA
AGGGCGTCGGACCGCTTCTCTACGTCCGCGTCAACGACCTCTCGACCGCTCTTACCGACGCTGATCTGGAGGCAGTGATG
CCTGCTGCGCCCGACGGCATCATGCTGCCGAAGGCCAATCATGCCGATCATGTCACGGAACTCAGCGCGCGTCTGTGCTT
GCTCGAAGCTCGTCATCGCATTGCCGAGGGCGAGACACGCATCCTGCCGCTCATCACGGAAACCGCCGAAGGTGTACTTA
ACGCGCCGACACACGCCCGCCCGAACACCCGCCTGTCTGGCTTGACCTGGGGCGCCGAGGATCTTTCTGCCTCGGTTGGA
GCACTAGTCGCGCGCGACTATCGAGGCTGCTATACGGCGCTCTTCCAACACGCCCGAACGATGGCAATCCTTGCGGCGGC
CGCTGCCGGCGTGCCTGCGGTCGATACCGTTTATCCCGATTTCCGCAACGAGGAAGGCTTTGCCAGCGATTGCGCCGAGG
CAGTCCGCGACGGGTTCACCGCCAAAATGGCGATCCATCCGGGGCAGGTTTCCGTCATCAACGCCGTGTTCACGCCCTCG
CCTGAAGCTGTGGCCCAAGCGAAGGAGGTGGTTGAGGCCTTCGCTAGGTCAGTCGGTGCTGGTGTCATCTCGCTCAACGG
CAAGATGTACGATCGTCCCCATTTGCGTCTGGCTGAGCGGCTTCTTGCACGGCTGACCTTGGCTTTATGA

Upstream 100 bases:

>100_bases
GCGGCATCGTGGAGTTTGAGCACCGCGCTTATAACCAGAACGACGTTCTGGTCGCGAAGACTATCCGGCAGGCCATGATC
CGGAAGAGGCACGCTTGACC

Downstream 100 bases:

>100_bases
CCGGCATTTCCCGATGTGTTGGAGCGCATGTCCCGACCACCCACATCCTCCCAAGATTGGTCACCTAGCGGGCTCACAAA
GAAGAAGGCTACCAAGTTGC

Product: citrate lyase beta-subunit

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRSLLFVPGDSARKQDKAMETGADLLILDLEDSVALSAKEDARKLTRAFVERNKGVGPLLYVRVNDLSTALTDADLEAVM
PAAPDGIMLPKANHADHVTELSARLCLLEARHRIAEGETRILPLITETAEGVLNAPTHARPNTRLSGLTWGAEDLSASVG
ALVARDYRGCYTALFQHARTMAILAAAAAGVPAVDTVYPDFRNEEGFASDCAEAVRDGFTAKMAIHPGQVSVINAVFTPS
PEAVAQAKEVVEAFARSVGAGVISLNGKMYDRPHLRLAERLLARLTLAL

Sequences:

>Translated_289_residues
MRSLLFVPGDSARKQDKAMETGADLLILDLEDSVALSAKEDARKLTRAFVERNKGVGPLLYVRVNDLSTALTDADLEAVM
PAAPDGIMLPKANHADHVTELSARLCLLEARHRIAEGETRILPLITETAEGVLNAPTHARPNTRLSGLTWGAEDLSASVG
ALVARDYRGCYTALFQHARTMAILAAAAAGVPAVDTVYPDFRNEEGFASDCAEAVRDGFTAKMAIHPGQVSVINAVFTPS
PEAVAQAKEVVEAFARSVGAGVISLNGKMYDRPHLRLAERLLARLTLAL
>Mature_289_residues
MRSLLFVPGDSARKQDKAMETGADLLILDLEDSVALSAKEDARKLTRAFVERNKGVGPLLYVRVNDLSTALTDADLEAVM
PAAPDGIMLPKANHADHVTELSARLCLLEARHRIAEGETRILPLITETAEGVLNAPTHARPNTRLSGLTWGAEDLSASVG
ALVARDYRGCYTALFQHARTMAILAAAAAGVPAVDTVYPDFRNEEGFASDCAEAVRDGFTAKMAIHPGQVSVINAVFTPS
PEAVAQAKEVVEAFARSVGAGVISLNGKMYDRPHLRLAERLLARLTLAL

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=291, Percent_Identity=31.6151202749141, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI87081764, Length=289, Percent_Identity=33.9100346020761, Blast_Score=124, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17557458, Length=290, Percent_Identity=32.0689655172414, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 30921; Mature: 30921

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLLFVPGDSARKQDKAMETGADLLILDLEDSVALSAKEDARKLTRAFVERNKGVGPLL
CCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHCCCCCCEE
YVRVNDLSTALTDADLEAVMPAAPDGIMLPKANHADHVTELSARLCLLEARHRIAEGETR
EEEECCHHHHHHHCCHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
ILPLITETAEGVLNAPTHARPNTRLSGLTWGAEDLSASVGALVARDYRGCYTALFQHART
EEEEEHHHHHHHCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAILAAAAAGVPAVDTVYPDFRNEEGFASDCAEAVRDGFTAKMAIHPGQVSVINAVFTPS
HHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCC
PEAVAQAKEVVEAFARSVGAGVISLNGKMYDRPHLRLAERLLARLTLAL
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSLLFVPGDSARKQDKAMETGADLLILDLEDSVALSAKEDARKLTRAFVERNKGVGPLL
CCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHCCCCCCEE
YVRVNDLSTALTDADLEAVMPAAPDGIMLPKANHADHVTELSARLCLLEARHRIAEGETR
EEEECCHHHHHHHCCHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
ILPLITETAEGVLNAPTHARPNTRLSGLTWGAEDLSASVGALVARDYRGCYTALFQHART
EEEEEHHHHHHHCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAILAAAAAGVPAVDTVYPDFRNEEGFASDCAEAVRDGFTAKMAIHPGQVSVINAVFTPS
HHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCC
PEAVAQAKEVVEAFARSVGAGVISLNGKMYDRPHLRLAERLLARLTLAL
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2205500; 7830578 [H]