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The map label for this gene is bioB [H]

Identifier: 13475019

GI number: 13475019

Start: 4856277

End: 4857545

Strand: Reverse

Name: bioB [H]

Synonym: mll6007

Alternate gene names: 13475019

Gene position: 4857545-4856277 (Counterclockwise)

Preceding gene: 13475020

Following gene: 13475018

Centisome position: 69.04

GC content: 64.22

Gene sequence:

>1269_bases
ATGCCTGAAGGTCACACCTTCGATGGCCACCAGATGTGGACCCTGGAGAAGGCTCGGGCTCTACACGACGCTCCCTTCAA
CAATCTCCTCTTCCTGGCGCAAACCGTGCACCGCGAGAACTTCGATCCCAACAAGGTCCAGCTGAGCCGGCTTCTCAGCA
TCAAGACGGGCGGCTGCCCGGAGGATTGCGGCTATTGCAGCCAGTCGGCGCATCACGAAAGCGGGTTGAAGGCGTCGAAG
CTGATGGAGGTCCGGCGCGTCATCGCCGAGGCGACCAAGGCACGCGATGCCGGCGCCACCCGCTATTGCATGGGCGCGGC
CTGGCGAAACCCGAAGGCGCGCGACATGGACGCGGTGGTGGCGATGGTCGAGGGTGTCAAGGCACTCGGCATGGAGACCT
GCATGACGCTCGGCATGCTCGATCTTGAGCAGGCCGCTCGTCTGAAACAGGCCGGTCTCGACTACTACAACCACAACATC
GACACCTCCGAGCGCTATTACTCAGAGATCATCTCGACCCGGACATTTGCCGACCGGCTGGACACGCTCGCCAATGTCCG
CGACAGCGGCATCAAGGTCTGTTGCGGCGGCATTGTCGGCATGGGCGAAGAGCCGGTGGACCGGATCGACATGCTGGTGA
CGCTGGCCAACCTGCCGGAGCATCCCGAAAGCGTTCCGATCAACATGCTGATCCCGATCGAGGGCACCCCGCTTGCCGAA
GCCAAGCCCATCGAGCCGATCGAATTCGTGCGCGTGATCGCCCTGGCGCGCATCATGATGCCGAAATCGCATGTGCGTCT
TTCCGCCGGCCGCACCGCCATGACCGACGAGATGCAGGCACTGTGTTTCTTTGCCGGCGCCAATTCGATCTTCGTCGGCG
ACACGCTGCTGACGGCGGACAATCCCGGCGAGGACAAGGACATTCTGCTGTTTCGGCGCCTCGGCATGGAGCCAATGGGC
GCAATGAACGGGGCGCTTCTTGCCCGGTATGGCCGGTCCTTGCGCGGACTGGCGCGCAAGGACCGGCTGCGGACGCTGGC
ACCGCGCGCCGGGCTCGACTTCTCGTCGAACGACTATCTCGGACTTGCCTCCTGCAAAAGACTTGGCGAGGCGGTTGCGG
CGGCGATTGCGCGGGGCACGCCGGTGGGCGCCACCGGATCGGCAACGCGCCGGAACACGAGGCGCTGGAGTCGGACGCTG
CGGCGTTTTTCGGAGCCGATCGCGCCCTGTTCTTCGGCAGCGGCTACATCGCCAACTTCGCTCTGCTGA

Upstream 100 bases:

>100_bases
TCGCCGAGGAGAAGGAGGATGGCTGATATTTCGTCACTGACAGCAGTTCGCCACAACACGGAACAGGATGGAGATGCACG
CAGCGATTTCAGAAAAGACC

Downstream 100 bases:

>100_bases
CGGCCCTGCCGCAGAAGGGCGACCTGCTGGTGCTCGACCAGCTCGCCCATGCCAGCATGCATGAGGGCGCGCAAGCCGGA
CGCGCCGAGTTCAAGCTCGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPEGHTFDGHQMWTLEKARALHDAPFNNLLFLAQTVHRENFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHESGLKASK
LMEVRRVIAEATKARDAGATRYCMGAAWRNPKARDMDAVVAMVEGVKALGMETCMTLGMLDLEQAARLKQAGLDYYNHNI
DTSERYYSEIISTRTFADRLDTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPIEGTPLAE
AKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDKDILLFRRLGMEPMG
AMNGALLARYGRSLRGLARKDRLRTLAPRAGLDFSSNDYLGLASCKRLGEAVAAAIARGTPVGATGSATRRNTRRWSRTL
RRFSEPIAPCSSAAATSPTSLC

Sequences:

>Translated_422_residues
MPEGHTFDGHQMWTLEKARALHDAPFNNLLFLAQTVHRENFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHESGLKASK
LMEVRRVIAEATKARDAGATRYCMGAAWRNPKARDMDAVVAMVEGVKALGMETCMTLGMLDLEQAARLKQAGLDYYNHNI
DTSERYYSEIISTRTFADRLDTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPIEGTPLAE
AKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDKDILLFRRLGMEPMG
AMNGALLARYGRSLRGLARKDRLRTLAPRAGLDFSSNDYLGLASCKRLGEAVAAAIARGTPVGATGSATRRNTRRWSRTL
RRFSEPIAPCSSAAATSPTSLC
>Mature_421_residues
PEGHTFDGHQMWTLEKARALHDAPFNNLLFLAQTVHRENFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHESGLKASKL
MEVRRVIAEATKARDAGATRYCMGAAWRNPKARDMDAVVAMVEGVKALGMETCMTLGMLDLEQAARLKQAGLDYYNHNID
TSERYYSEIISTRTFADRLDTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPIEGTPLAEA
KPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDKDILLFRRLGMEPMGA
MNGALLARYGRSLRGLARKDRLRTLAPRAGLDFSSNDYLGLASCKRLGEAVAAAIARGTPVGATGSATRRNTRRWSRTLR
RFSEPIAPCSSAAATSPTSLC

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=60.4575163398693, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321725, Length=329, Percent_Identity=48.0243161094225, Blast_Score=325, Evalue=6e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 46047; Mature: 45915

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGHTFDGHQMWTLEKARALHDAPFNNLLFLAQTVHRENFDPNKVQLSRLLSIKTGGCP
CCCCCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
EDCGYCSQSAHHESGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKARDMDAVV
HHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHH
AMVEGVKALGMETCMTLGMLDLEQAARLKQAGLDYYNHNIDTSERYYSEIISTRTFADRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
DTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPIEGTPLAE
HHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCC
AKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTAD
CCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHCCCCEEEECCEEEECC
NPGEDKDILLFRRLGMEPMGAMNGALLARYGRSLRGLARKDRLRTLAPRAGLDFSSNDYL
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GLASCKRLGEAVAAAIARGTPVGATGSATRRNTRRWSRTLRRFSEPIAPCSSAAATSPTS
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LC
CC
>Mature Secondary Structure 
PEGHTFDGHQMWTLEKARALHDAPFNNLLFLAQTVHRENFDPNKVQLSRLLSIKTGGCP
CCCCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
EDCGYCSQSAHHESGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKARDMDAVV
HHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHH
AMVEGVKALGMETCMTLGMLDLEQAARLKQAGLDYYNHNIDTSERYYSEIISTRTFADRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
DTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPIEGTPLAE
HHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCC
AKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTAD
CCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHCCCCEEEECCEEEECC
NPGEDKDILLFRRLGMEPMGAMNGALLARYGRSLRGLARKDRLRTLAPRAGLDFSSNDYL
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GLASCKRLGEAVAAAIARGTPVGATGSATRRNTRRWSRTLRRFSEPIAPCSSAAATSPTS
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LC
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]